Amino acid dipepetide frequency for Pirellula sp. SH-Sr6A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.956AlaAla: 8.956 ± 0.099
1.201AlaCys: 1.201 ± 0.029
4.631AlaAsp: 4.631 ± 0.053
5.366AlaGlu: 5.366 ± 0.074
3.351AlaPhe: 3.351 ± 0.044
6.613AlaGly: 6.613 ± 0.071
1.475AlaHis: 1.475 ± 0.031
6.135AlaIle: 6.135 ± 0.062
4.646AlaLys: 4.646 ± 0.064
8.024AlaLeu: 8.024 ± 0.063
2.706AlaMet: 2.706 ± 0.041
3.297AlaAsn: 3.297 ± 0.046
4.293AlaPro: 4.293 ± 0.061
3.071AlaGln: 3.071 ± 0.045
5.159AlaArg: 5.159 ± 0.062
6.952AlaSer: 6.952 ± 0.067
5.341AlaThr: 5.341 ± 0.089
5.887AlaVal: 5.887 ± 0.063
1.599AlaTrp: 1.599 ± 0.035
2.007AlaTyr: 2.007 ± 0.033
0.0AlaXaa: 0.0 ± 0.0
Cys
0.787CysAla: 0.787 ± 0.021
0.264CysCys: 0.264 ± 0.015
0.764CysAsp: 0.764 ± 0.024
0.777CysGlu: 0.777 ± 0.021
0.5CysPhe: 0.5 ± 0.017
0.934CysGly: 0.934 ± 0.028
0.425CysHis: 0.425 ± 0.017
0.603CysIle: 0.603 ± 0.018
0.464CysLys: 0.464 ± 0.017
1.192CysLeu: 1.192 ± 0.031
0.239CysMet: 0.239 ± 0.01
0.397CysAsn: 0.397 ± 0.016
0.57CysPro: 0.57 ± 0.018
0.422CysGln: 0.422 ± 0.016
0.798CysArg: 0.798 ± 0.021
0.861CysSer: 0.861 ± 0.021
0.578CysThr: 0.578 ± 0.016
0.837CysVal: 0.837 ± 0.021
0.205CysTrp: 0.205 ± 0.011
0.314CysTyr: 0.314 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
5.574AspAla: 5.574 ± 0.062
0.678AspCys: 0.678 ± 0.019
2.901AspAsp: 2.901 ± 0.046
3.654AspGlu: 3.654 ± 0.052
2.255AspPhe: 2.255 ± 0.033
4.706AspGly: 4.706 ± 0.064
1.194AspHis: 1.194 ± 0.024
2.541AspIle: 2.541 ± 0.037
1.815AspLys: 1.815 ± 0.039
5.561AspLeu: 5.561 ± 0.062
0.972AspMet: 0.972 ± 0.023
1.45AspAsn: 1.45 ± 0.036
3.309AspPro: 3.309 ± 0.049
2.148AspGln: 2.148 ± 0.034
4.42AspArg: 4.42 ± 0.049
3.921AspSer: 3.921 ± 0.051
2.395AspThr: 2.395 ± 0.049
3.511AspVal: 3.511 ± 0.057
1.197AspTrp: 1.197 ± 0.027
1.434AspTyr: 1.434 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
5.444GluAla: 5.444 ± 0.057
0.656GluCys: 0.656 ± 0.022
2.86GluAsp: 2.86 ± 0.043
3.932GluGlu: 3.932 ± 0.068
2.393GluPhe: 2.393 ± 0.037
4.017GluGly: 4.017 ± 0.048
1.33GluHis: 1.33 ± 0.029
3.678GluIle: 3.678 ± 0.049
3.123GluLys: 3.123 ± 0.046
6.816GluLeu: 6.816 ± 0.076
1.569GluMet: 1.569 ± 0.032
2.144GluAsn: 2.144 ± 0.037
2.824GluPro: 2.824 ± 0.045
3.03GluGln: 3.03 ± 0.049
4.451GluArg: 4.451 ± 0.064
5.081GluSer: 5.081 ± 0.063
3.339GluThr: 3.339 ± 0.048
3.972GluVal: 3.972 ± 0.048
1.038GluTrp: 1.038 ± 0.025
1.478GluTyr: 1.478 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
3.681PheAla: 3.681 ± 0.044
0.507PheCys: 0.507 ± 0.017
2.568PheAsp: 2.568 ± 0.044
2.498PheGlu: 2.498 ± 0.033
1.476PhePhe: 1.476 ± 0.029
3.261PheGly: 3.261 ± 0.044
0.856PheHis: 0.856 ± 0.025
1.47PheIle: 1.47 ± 0.032
1.035PheLys: 1.035 ± 0.025
3.68PheLeu: 3.68 ± 0.048
0.648PheMet: 0.648 ± 0.019
1.099PheAsn: 1.099 ± 0.029
1.768PhePro: 1.768 ± 0.032
1.671PheGln: 1.671 ± 0.03
2.514PheArg: 2.514 ± 0.037
2.588PheSer: 2.588 ± 0.042
1.895PheThr: 1.895 ± 0.041
2.819PheVal: 2.819 ± 0.04
0.582PheTrp: 0.582 ± 0.015
0.944PheTyr: 0.944 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
6.014GlyAla: 6.014 ± 0.085
1.02GlyCys: 1.02 ± 0.026
3.891GlyAsp: 3.891 ± 0.049
4.362GlyGlu: 4.362 ± 0.054
3.018GlyPhe: 3.018 ± 0.039
6.129GlyGly: 6.129 ± 0.105
1.442GlyHis: 1.442 ± 0.033
4.332GlyIle: 4.332 ± 0.047
3.868GlyLys: 3.868 ± 0.055
6.938GlyLeu: 6.938 ± 0.06
1.995GlyMet: 1.995 ± 0.04
2.704GlyAsn: 2.704 ± 0.05
3.0GlyPro: 3.0 ± 0.061
2.584GlyGln: 2.584 ± 0.041
4.576GlyArg: 4.576 ± 0.057
5.336GlySer: 5.336 ± 0.078
4.53GlyThr: 4.53 ± 0.093
5.23GlyVal: 5.23 ± 0.06
1.406GlyTrp: 1.406 ± 0.031
2.117GlyTyr: 2.117 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
1.943HisAla: 1.943 ± 0.035
0.374HisCys: 0.374 ± 0.013
1.104HisAsp: 1.104 ± 0.024
1.199HisGlu: 1.199 ± 0.031
0.936HisPhe: 0.936 ± 0.022
1.687HisGly: 1.687 ± 0.032
0.58HisHis: 0.58 ± 0.018
0.877HisIle: 0.877 ± 0.021
0.565HisLys: 0.565 ± 0.018
2.156HisLeu: 2.156 ± 0.041
0.36HisMet: 0.36 ± 0.014
0.553HisAsn: 0.553 ± 0.018
1.5HisPro: 1.5 ± 0.03
0.859HisGln: 0.859 ± 0.025
1.779HisArg: 1.779 ± 0.038
1.428HisSer: 1.428 ± 0.028
0.896HisThr: 0.896 ± 0.022
1.347HisVal: 1.347 ± 0.025
0.504HisTrp: 0.504 ± 0.019
0.602HisTyr: 0.602 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.404IleAla: 6.404 ± 0.073
0.625IleCys: 0.625 ± 0.019
4.033IleAsp: 4.033 ± 0.055
4.242IleGlu: 4.242 ± 0.052
1.461IlePhe: 1.461 ± 0.03
4.33IleGly: 4.33 ± 0.052
1.245IleHis: 1.245 ± 0.027
1.881IleIle: 1.881 ± 0.036
1.578IleLys: 1.578 ± 0.029
4.895IleLeu: 4.895 ± 0.052
0.682IleMet: 0.682 ± 0.021
1.489IleAsn: 1.489 ± 0.034
3.138IlePro: 3.138 ± 0.042
2.333IleGln: 2.333 ± 0.039
3.864IleArg: 3.864 ± 0.047
3.307IleSer: 3.307 ± 0.039
2.534IleThr: 2.534 ± 0.061
4.018IleVal: 4.018 ± 0.05
0.67IleTrp: 0.67 ± 0.02
1.204IleTyr: 1.204 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
3.484LysAla: 3.484 ± 0.048
0.35LysCys: 0.35 ± 0.016
1.993LysAsp: 1.993 ± 0.039
2.706LysGlu: 2.706 ± 0.043
1.191LysPhe: 1.191 ± 0.03
2.597LysGly: 2.597 ± 0.042
0.892LysHis: 0.892 ± 0.021
2.212LysIle: 2.212 ± 0.037
2.252LysLys: 2.252 ± 0.045
4.322LysLeu: 4.322 ± 0.057
0.991LysMet: 0.991 ± 0.025
1.399LysAsn: 1.399 ± 0.031
2.581LysPro: 2.581 ± 0.037
1.886LysGln: 1.886 ± 0.04
2.969LysArg: 2.969 ± 0.044
3.018LysSer: 3.018 ± 0.047
2.363LysThr: 2.363 ± 0.035
2.658LysVal: 2.658 ± 0.047
0.684LysTrp: 0.684 ± 0.024
0.907LysTyr: 0.907 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
10.054LeuAla: 10.054 ± 0.082
1.188LeuCys: 1.188 ± 0.03
5.815LeuAsp: 5.815 ± 0.061
6.447LeuGlu: 6.447 ± 0.076
3.612LeuPhe: 3.612 ± 0.053
7.227LeuGly: 7.227 ± 0.067
2.168LeuHis: 2.168 ± 0.036
4.707LeuIle: 4.707 ± 0.046
3.781LeuLys: 3.781 ± 0.051
9.95LeuLeu: 9.95 ± 0.094
1.947LeuMet: 1.947 ± 0.032
2.855LeuAsn: 2.855 ± 0.043
5.556LeuPro: 5.556 ± 0.062
4.294LeuGln: 4.294 ± 0.06
6.746LeuArg: 6.746 ± 0.072
7.002LeuSer: 7.002 ± 0.07
5.034LeuThr: 5.034 ± 0.058
6.786LeuVal: 6.786 ± 0.059
1.324LeuTrp: 1.324 ± 0.03
2.124LeuTyr: 2.124 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
2.134MetAla: 2.134 ± 0.035
0.193MetCys: 0.193 ± 0.01
1.179MetAsp: 1.179 ± 0.029
1.386MetGlu: 1.386 ± 0.029
0.727MetPhe: 0.727 ± 0.021
1.637MetGly: 1.637 ± 0.038
0.484MetHis: 0.484 ± 0.016
1.191MetIle: 1.191 ± 0.023
1.073MetLys: 1.073 ± 0.023
2.282MetLeu: 2.282 ± 0.04
0.572MetMet: 0.572 ± 0.019
0.853MetAsn: 0.853 ± 0.019
1.227MetPro: 1.227 ± 0.027
1.04MetGln: 1.04 ± 0.024
1.53MetArg: 1.53 ± 0.031
1.57MetSer: 1.57 ± 0.029
1.32MetThr: 1.32 ± 0.022
1.549MetVal: 1.549 ± 0.032
0.215MetTrp: 0.215 ± 0.012
0.352MetTyr: 0.352 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.17AsnAla: 3.17 ± 0.05
0.351AsnCys: 0.351 ± 0.013
1.847AsnAsp: 1.847 ± 0.044
1.989AsnGlu: 1.989 ± 0.036
1.006AsnPhe: 1.006 ± 0.025
2.833AsnGly: 2.833 ± 0.053
0.762AsnHis: 0.762 ± 0.019
1.447AsnIle: 1.447 ± 0.029
1.115AsnLys: 1.115 ± 0.029
3.034AsnLeu: 3.034 ± 0.039
0.625AsnMet: 0.625 ± 0.016
1.122AsnAsn: 1.122 ± 0.04
2.279AsnPro: 2.279 ± 0.04
1.454AsnGln: 1.454 ± 0.03
2.553AsnArg: 2.553 ± 0.04
2.181AsnSer: 2.181 ± 0.045
1.655AsnThr: 1.655 ± 0.047
2.208AsnVal: 2.208 ± 0.044
0.611AsnTrp: 0.611 ± 0.019
0.889AsnTyr: 0.889 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
4.599ProAla: 4.599 ± 0.069
0.446ProCys: 0.446 ± 0.016
3.168ProAsp: 3.168 ± 0.045
3.653ProGlu: 3.653 ± 0.047
2.041ProPhe: 2.041 ± 0.036
3.688ProGly: 3.688 ± 0.061
1.1ProHis: 1.1 ± 0.024
3.047ProIle: 3.047 ± 0.044
2.364ProLys: 2.364 ± 0.039
4.416ProLeu: 4.416 ± 0.053
1.196ProMet: 1.196 ± 0.024
2.204ProAsn: 2.204 ± 0.037
2.851ProPro: 2.851 ± 0.052
1.787ProGln: 1.787 ± 0.033
2.75ProArg: 2.75 ± 0.041
4.429ProSer: 4.429 ± 0.059
3.273ProThr: 3.273 ± 0.044
3.606ProVal: 3.606 ± 0.053
0.853ProTrp: 0.853 ± 0.025
1.205ProTyr: 1.205 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.652GlnAla: 3.652 ± 0.053
0.469GlnCys: 0.469 ± 0.018
1.778GlnAsp: 1.778 ± 0.03
2.182GlnGlu: 2.182 ± 0.035
1.575GlnPhe: 1.575 ± 0.029
2.594GlnGly: 2.594 ± 0.045
0.848GlnHis: 0.848 ± 0.022
2.345GlnIle: 2.345 ± 0.036
1.71GlnLys: 1.71 ± 0.035
4.232GlnLeu: 4.232 ± 0.051
0.92GlnMet: 0.92 ± 0.022
1.289GlnAsn: 1.289 ± 0.028
2.142GlnPro: 2.142 ± 0.04
1.916GlnGln: 1.916 ± 0.045
2.912GlnArg: 2.912 ± 0.047
3.458GlnSer: 3.458 ± 0.051
2.164GlnThr: 2.164 ± 0.039
2.704GlnVal: 2.704 ± 0.035
0.769GlnTrp: 0.769 ± 0.022
0.939GlnTyr: 0.939 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
4.915ArgAla: 4.915 ± 0.057
0.883ArgCys: 0.883 ± 0.021
3.683ArgAsp: 3.683 ± 0.052
4.412ArgGlu: 4.412 ± 0.06
3.063ArgPhe: 3.063 ± 0.037
4.235ArgGly: 4.235 ± 0.048
1.267ArgHis: 1.267 ± 0.026
4.209ArgIle: 4.209 ± 0.042
2.797ArgLys: 2.797 ± 0.045
7.059ArgLeu: 7.059 ± 0.07
1.857ArgMet: 1.857 ± 0.032
2.278ArgAsn: 2.278 ± 0.035
2.88ArgPro: 2.88 ± 0.05
2.482ArgGln: 2.482 ± 0.038
4.51ArgArg: 4.51 ± 0.061
4.901ArgSer: 4.901 ± 0.055
3.418ArgThr: 3.418 ± 0.045
4.519ArgVal: 4.519 ± 0.055
1.449ArgTrp: 1.449 ± 0.032
1.891ArgTyr: 1.891 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
5.519SerAla: 5.519 ± 0.057
0.715SerCys: 0.715 ± 0.019
4.201SerAsp: 4.201 ± 0.053
4.402SerGlu: 4.402 ± 0.053
2.77SerPhe: 2.77 ± 0.043
5.672SerGly: 5.672 ± 0.078
1.566SerHis: 1.566 ± 0.028
4.408SerIle: 4.408 ± 0.052
3.158SerLys: 3.158 ± 0.05
7.51SerLeu: 7.51 ± 0.072
1.799SerMet: 1.799 ± 0.03
2.709SerAsn: 2.709 ± 0.052
4.013SerPro: 4.013 ± 0.051
3.034SerGln: 3.034 ± 0.042
4.298SerArg: 4.298 ± 0.05
5.424SerSer: 5.424 ± 0.076
3.982SerThr: 3.982 ± 0.063
4.942SerVal: 4.942 ± 0.058
1.109SerTrp: 1.109 ± 0.023
1.636SerTyr: 1.636 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
4.665ThrAla: 4.665 ± 0.067
0.52ThrCys: 0.52 ± 0.017
2.873ThrAsp: 2.873 ± 0.053
3.002ThrGlu: 3.002 ± 0.038
1.963ThrPhe: 1.963 ± 0.043
4.437ThrGly: 4.437 ± 0.088
1.136ThrHis: 1.136 ± 0.029
3.267ThrIle: 3.267 ± 0.059
2.216ThrLys: 2.216 ± 0.04
5.39ThrLeu: 5.39 ± 0.059
1.103ThrMet: 1.103 ± 0.024
1.861ThrAsn: 1.861 ± 0.053
3.371ThrPro: 3.371 ± 0.047
1.97ThrGln: 1.97 ± 0.032
3.048ThrArg: 3.048 ± 0.046
3.7ThrSer: 3.7 ± 0.067
3.058ThrThr: 3.058 ± 0.066
3.763ThrVal: 3.763 ± 0.08
0.855ThrTrp: 0.855 ± 0.024
1.307ThrTyr: 1.307 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
6.633ValAla: 6.633 ± 0.07
0.908ValCys: 0.908 ± 0.029
4.103ValAsp: 4.103 ± 0.05
4.555ValGlu: 4.555 ± 0.05
2.506ValPhe: 2.506 ± 0.039
4.912ValGly: 4.912 ± 0.058
1.537ValHis: 1.537 ± 0.03
3.44ValIle: 3.44 ± 0.042
2.267ValLys: 2.267 ± 0.032
6.775ValLeu: 6.775 ± 0.054
1.351ValMet: 1.351 ± 0.029
1.995ValAsn: 1.995 ± 0.043
3.475ValPro: 3.475 ± 0.045
2.751ValGln: 2.751 ± 0.042
4.602ValArg: 4.602 ± 0.047
4.83ValSer: 4.83 ± 0.066
3.671ValThr: 3.671 ± 0.075
5.227ValVal: 5.227 ± 0.061
1.106ValTrp: 1.106 ± 0.024
1.603ValTyr: 1.603 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.07TrpAla: 1.07 ± 0.026
0.209TrpCys: 0.209 ± 0.011
0.89TrpAsp: 0.89 ± 0.023
0.916TrpGlu: 0.916 ± 0.025
0.652TrpPhe: 0.652 ± 0.019
1.07TrpGly: 1.07 ± 0.027
0.366TrpHis: 0.366 ± 0.016
1.253TrpIle: 1.253 ± 0.025
0.911TrpLys: 0.911 ± 0.025
1.87TrpLeu: 1.87 ± 0.035
0.548TrpMet: 0.548 ± 0.016
0.739TrpAsn: 0.739 ± 0.02
0.683TrpPro: 0.683 ± 0.022
0.736TrpGln: 0.736 ± 0.02
1.058TrpArg: 1.058 ± 0.029
1.251TrpSer: 1.251 ± 0.024
0.933TrpThr: 0.933 ± 0.025
1.088TrpVal: 1.088 ± 0.027
0.292TrpTrp: 0.292 ± 0.014
0.389TrpTyr: 0.389 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.011TyrAla: 2.011 ± 0.036
0.376TyrCys: 0.376 ± 0.013
1.355TyrAsp: 1.355 ± 0.034
1.498TyrGlu: 1.498 ± 0.03
1.064TyrPhe: 1.064 ± 0.024
1.911TyrGly: 1.911 ± 0.035
0.575TyrHis: 0.575 ± 0.016
0.89TyrIle: 0.89 ± 0.022
0.718TyrLys: 0.718 ± 0.023
2.517TyrLeu: 2.517 ± 0.041
0.434TyrMet: 0.434 ± 0.018
0.695TyrAsn: 0.695 ± 0.021
1.216TyrPro: 1.216 ± 0.03
1.13TyrGln: 1.13 ± 0.027
2.172TyrArg: 2.172 ± 0.035
1.629TyrSer: 1.629 ± 0.037
1.125TyrThr: 1.125 ± 0.032
1.574TyrVal: 1.574 ± 0.028
0.455TyrTrp: 0.455 ± 0.015
0.731TyrTyr: 0.731 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5207 proteins (1912954 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski