Circovirus-like genome DCCV-11
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A190WHL5|A0A190WHL5_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-11 OX=1788439 PE=4 SV=1
MM1 pKa = 7.65 CKK3 pKa = 10.17 GVNNFLQVGKK13 pKa = 10.37 QNSVDD18 pKa = 3.55 THH20 pKa = 6.11 SKK22 pKa = 10.2 KK23 pKa = 10.16 HH24 pKa = 4.92 MLEE27 pKa = 4.44 HH28 pKa = 6.88 GEE30 pKa = 4.36 SKK32 pKa = 10.52 HH33 pKa = 4.62 GTEE36 pKa = 4.12 VFPVNILQSDD46 pKa = 4.1 VSSQRR51 pKa = 11.84 RR52 pKa = 11.84 VEE54 pKa = 4.17 TGVGISLIIKK64 pKa = 10.27 RR65 pKa = 11.84 LDD67 pKa = 3.24 TLEE70 pKa = 4.78 RR71 pKa = 11.84 SVSYY75 pKa = 10.49 LVEE78 pKa = 4.57 LSQKK82 pKa = 10.57 LYY84 pKa = 11.13 EE85 pKa = 4.33 EE86 pKa = 5.16 DD87 pKa = 4.27 SYY89 pKa = 12.24 SSDD92 pKa = 3.29 EE93 pKa = 4.81 DD94 pKa = 4.22 SDD96 pKa = 4.02 FQEE99 pKa = 4.72 SKK101 pKa = 11.26
Molecular weight: 11.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.202
IPC2_protein 5.232
IPC_protein 5.13
Toseland 5.194
ProMoST 5.321
Dawson 5.181
Bjellqvist 5.283
Wikipedia 5.067
Rodwell 5.118
Grimsley 5.156
Solomon 5.181
Lehninger 5.143
Nozaki 5.334
DTASelect 5.474
Thurlkill 5.219
EMBOSS 5.169
Sillero 5.41
Patrickios 4.202
IPC_peptide 5.194
IPC2_peptide 5.41
IPC2.peptide.svr19 5.404
Protein with the highest isoelectric point:
>tr|A0A190WHL5|A0A190WHL5_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-11 OX=1788439 PE=4 SV=1
MM1 pKa = 7.75 PPLRR5 pKa = 11.84 LPFGSRR11 pKa = 11.84 PGLHH15 pKa = 6.37 WPQSSVDD22 pKa = 3.44 HH23 pKa = 5.13 YY24 pKa = 9.28 VQRR27 pKa = 11.84 RR28 pKa = 11.84 KK29 pKa = 9.9 QLLASGKK36 pKa = 10.34 AKK38 pKa = 9.74 FRR40 pKa = 11.84 RR41 pKa = 11.84 YY42 pKa = 9.43 ALKK45 pKa = 10.3 KK46 pKa = 9.99 AYY48 pKa = 10.05 VGAWRR53 pKa = 11.84 KK54 pKa = 5.86 QARR57 pKa = 11.84 YY58 pKa = 9.78 GGFSGKK64 pKa = 9.99 YY65 pKa = 7.09 PPKK68 pKa = 10.18 RR69 pKa = 11.84 RR70 pKa = 11.84 LFPAQGRR77 pKa = 11.84 NRR79 pKa = 11.84 SRR81 pKa = 11.84 DD82 pKa = 3.37 QSHH85 pKa = 5.7 NQALRR90 pKa = 11.84 HH91 pKa = 5.34 LRR93 pKa = 11.84 ALSLLFGRR101 pKa = 11.84 TKK103 pKa = 10.58 SKK105 pKa = 10.59 AVRR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 QLQLGRR116 pKa = 11.84 RR117 pKa = 11.84 LGLSGKK123 pKa = 9.73 QVDD126 pKa = 4.14 SYY128 pKa = 11.85 SRR130 pKa = 11.84 NANLL134 pKa = 4.26
Molecular weight: 15.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.213
IPC2_protein 10.789
IPC_protein 11.959
Toseland 12.106
ProMoST 12.588
Dawson 12.106
Bjellqvist 12.091
Wikipedia 12.574
Rodwell 11.871
Grimsley 12.149
Solomon 12.588
Lehninger 12.486
Nozaki 12.091
DTASelect 12.091
Thurlkill 12.106
EMBOSS 12.588
Sillero 12.106
Patrickios 11.579
IPC_peptide 12.588
IPC2_peptide 11.579
IPC2.peptide.svr19 9.042
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1309
101
466
261.8
29.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.889 ± 0.817
1.833 ± 0.42
5.806 ± 0.77
5.118 ± 1.017
2.827 ± 0.561
6.723 ± 0.34
1.757 ± 0.513
4.125 ± 1.138
6.494 ± 0.652
8.174 ± 1.175
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.063 ± 0.473
4.66 ± 1.229
4.889 ± 0.817
3.743 ± 0.73
9.167 ± 1.18
9.473 ± 2.099
7.716 ± 1.393
4.813 ± 0.662
1.451 ± 0.385
4.278 ± 0.958
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here