Rosellinia necatrix partitivirus 1-W8

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Betapartitivirus; Rosellinia necatrix virus 1

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q50LH7|Q50LH7_9VIRU Putative RNA-dependent RNA polymerase OS=Rosellinia necatrix partitivirus 1-W8 OX=235994 GN=RnPV1-W8 RdRp PE=4 SV=1
MM1 pKa = 7.34VLTIIRR7 pKa = 11.84DD8 pKa = 3.96YY9 pKa = 11.31LHH11 pKa = 6.83EE12 pKa = 4.47AQLRR16 pKa = 11.84LKK18 pKa = 10.78KK19 pKa = 9.93EE20 pKa = 3.53WQTFQKK26 pKa = 10.51SDD28 pKa = 3.56QEE30 pKa = 4.22SGYY33 pKa = 10.67SDD35 pKa = 4.73KK36 pKa = 11.48LPTDD40 pKa = 3.48YY41 pKa = 11.28DD42 pKa = 3.29LRR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 9.56YY47 pKa = 11.05DD48 pKa = 3.05SARR51 pKa = 11.84DD52 pKa = 3.49YY53 pKa = 11.28DD54 pKa = 4.02AEE56 pKa = 4.03KK57 pKa = 10.76HH58 pKa = 4.73KK59 pKa = 9.98TEE61 pKa = 4.36EE62 pKa = 4.19YY63 pKa = 9.47QHH65 pKa = 6.39NFALTHH71 pKa = 5.53EE72 pKa = 5.67RR73 pKa = 11.84YY74 pKa = 7.78TQMNADD80 pKa = 3.74RR81 pKa = 11.84NEE83 pKa = 3.72PFEE86 pKa = 4.16FYY88 pKa = 10.62RR89 pKa = 11.84PLEE92 pKa = 4.55DD93 pKa = 5.31NEE95 pKa = 4.58LPDD98 pKa = 4.08IRR100 pKa = 11.84FPAPGITVLPFRR112 pKa = 11.84YY113 pKa = 8.4HH114 pKa = 6.28TGQIVEE120 pKa = 4.6TTDD123 pKa = 3.36EE124 pKa = 4.64LPDD127 pKa = 3.61SGFSLHH133 pKa = 6.89PLIDD137 pKa = 3.89YY138 pKa = 7.55LTKK141 pKa = 10.54TKK143 pKa = 9.2WLHH146 pKa = 4.22YY147 pKa = 9.92RR148 pKa = 11.84PYY150 pKa = 9.33IDD152 pKa = 4.32KK153 pKa = 10.25YY154 pKa = 10.58CRR156 pKa = 11.84PLGTTNATFSDD167 pKa = 4.5FNRR170 pKa = 11.84EE171 pKa = 4.17QIPSAPIDD179 pKa = 3.58EE180 pKa = 4.57TRR182 pKa = 11.84KK183 pKa = 10.54NMVLPLVIYY192 pKa = 8.67FLNALPFLPIHH203 pKa = 6.13FVDD206 pKa = 3.87TRR208 pKa = 11.84FCGTPKK214 pKa = 9.21HH215 pKa = 5.49TATGYY220 pKa = 7.82FQRR223 pKa = 11.84FSTFFRR229 pKa = 11.84THH231 pKa = 6.45AYY233 pKa = 8.47YY234 pKa = 10.86ARR236 pKa = 11.84NKK238 pKa = 10.3LYY240 pKa = 10.82ALRR243 pKa = 11.84PTSKK247 pKa = 10.62GYY249 pKa = 10.42FFNTVYY255 pKa = 10.41EE256 pKa = 5.0FSRR259 pKa = 11.84TWMHH263 pKa = 6.86HH264 pKa = 5.15IKK266 pKa = 10.1EE267 pKa = 4.11HH268 pKa = 6.63GYY270 pKa = 9.81PFVPSHH276 pKa = 7.33DD277 pKa = 3.92ALDD280 pKa = 3.48NARR283 pKa = 11.84QYY285 pKa = 11.11RR286 pKa = 11.84IFMQKK291 pKa = 9.53HH292 pKa = 3.35VTMLFTRR299 pKa = 11.84NHH301 pKa = 5.6ISDD304 pKa = 3.41RR305 pKa = 11.84DD306 pKa = 3.63GFLKK310 pKa = 10.19QRR312 pKa = 11.84PVYY315 pKa = 10.56AVDD318 pKa = 5.14DD319 pKa = 4.05FFILCEE325 pKa = 3.98LMISFPLHH333 pKa = 5.26VMARR337 pKa = 11.84YY338 pKa = 8.26PINGIKK344 pKa = 9.75SCIMYY349 pKa = 10.29SFEE352 pKa = 4.6TIRR355 pKa = 11.84GSNRR359 pKa = 11.84YY360 pKa = 8.76LDD362 pKa = 4.17SIARR366 pKa = 11.84DD367 pKa = 4.32FISFFTIDD375 pKa = 3.27WSSFDD380 pKa = 3.25QRR382 pKa = 11.84VPRR385 pKa = 11.84VITDD389 pKa = 3.53IFWTDD394 pKa = 3.68FLRR397 pKa = 11.84QLIVINHH404 pKa = 7.12GYY406 pKa = 9.18QPTYY410 pKa = 10.22EE411 pKa = 4.15YY412 pKa = 10.0PAYY415 pKa = 9.4PDD417 pKa = 4.18LSEE420 pKa = 4.84HH421 pKa = 7.21DD422 pKa = 3.85LYY424 pKa = 11.69KK425 pKa = 10.87RR426 pKa = 11.84MNNLLHH432 pKa = 7.08FLHH435 pKa = 6.25TWYY438 pKa = 11.15NNMVFVTADD447 pKa = 3.04GFAYY451 pKa = 10.19LRR453 pKa = 11.84SAAGVPSGLLNTQYY467 pKa = 11.47LDD469 pKa = 3.57SFCNLFLIIDD479 pKa = 3.89GLFEE483 pKa = 4.64FGFTQAEE490 pKa = 4.07ILSIVFFIMGDD501 pKa = 3.72DD502 pKa = 3.58NSGFTMMDD510 pKa = 3.42IEE512 pKa = 5.1RR513 pKa = 11.84LTQFIEE519 pKa = 4.52FFEE522 pKa = 4.57SYY524 pKa = 10.53ALKK527 pKa = 10.14RR528 pKa = 11.84YY529 pKa = 10.52NMVLSKK535 pKa = 9.11TKK537 pKa = 10.71SVITTLRR544 pKa = 11.84SRR546 pKa = 11.84IEE548 pKa = 3.99TLSYY552 pKa = 10.03QCNGGNPKK560 pKa = 10.24RR561 pKa = 11.84PLGKK565 pKa = 10.38LIAQLCYY572 pKa = 9.87PEE574 pKa = 5.68HH575 pKa = 6.29GPKK578 pKa = 10.01DD579 pKa = 3.65KK580 pKa = 11.28YY581 pKa = 10.25MSARR585 pKa = 11.84AIGIAYY591 pKa = 8.94AAAAMDD597 pKa = 3.93EE598 pKa = 4.46EE599 pKa = 4.37FHH601 pKa = 6.37EE602 pKa = 5.0FCRR605 pKa = 11.84DD606 pKa = 2.95IYY608 pKa = 10.72HH609 pKa = 6.68TFLPYY614 pKa = 10.13AAPIDD619 pKa = 3.91EE620 pKa = 5.12HH621 pKa = 5.82TLSMATKK628 pKa = 9.94HH629 pKa = 5.69LPGYY633 pKa = 10.69FKK635 pKa = 10.56MLDD638 pKa = 3.7NIASEE643 pKa = 4.35IKK645 pKa = 10.13FDD647 pKa = 4.07SFPTLEE653 pKa = 4.26MVQDD657 pKa = 4.38KK658 pKa = 10.37YY659 pKa = 11.7SRR661 pKa = 11.84WQGYY665 pKa = 9.52LSHH668 pKa = 6.66KK669 pKa = 9.6PKK671 pKa = 10.51WNDD674 pKa = 2.64AHH676 pKa = 6.93FKK678 pKa = 10.16FLPEE682 pKa = 3.98TVPNNIKK689 pKa = 9.76TMTDD693 pKa = 3.15YY694 pKa = 11.13QLEE697 pKa = 4.57HH698 pKa = 7.24KK699 pKa = 10.49LDD701 pKa = 3.74TPVPHH706 pKa = 7.14SLFF709 pKa = 3.96

Molecular weight:
83.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q50LH7|Q50LH7_9VIRU Putative RNA-dependent RNA polymerase OS=Rosellinia necatrix partitivirus 1-W8 OX=235994 GN=RnPV1-W8 RdRp PE=4 SV=1
MM1 pKa = 7.7SSSRR5 pKa = 11.84YY6 pKa = 8.7NAAARR11 pKa = 11.84AATIAQKK18 pKa = 10.68SSSEE22 pKa = 3.92APKK25 pKa = 10.29EE26 pKa = 4.24SKK28 pKa = 11.08VPDD31 pKa = 3.5NSVDD35 pKa = 3.84FLTSYY40 pKa = 10.59KK41 pKa = 10.18AASSARR47 pKa = 11.84LSTANLFTVDD57 pKa = 3.9VLPDD61 pKa = 3.62FTPILMFIMFHH72 pKa = 7.03AIQLSSDD79 pKa = 2.73IDD81 pKa = 3.64NRR83 pKa = 11.84KK84 pKa = 8.73HH85 pKa = 6.2PKK87 pKa = 8.08ITTPTFVVYY96 pKa = 10.35CLSLVYY102 pKa = 10.25GYY104 pKa = 11.07FLLSDD109 pKa = 3.74MYY111 pKa = 11.17VRR113 pKa = 11.84PSASAPAQDD122 pKa = 5.69YY123 pKa = 10.81FDD125 pKa = 3.79QPYY128 pKa = 10.25KK129 pKa = 10.83KK130 pKa = 10.05QFSDD134 pKa = 3.55MLLGLYY140 pKa = 9.4VPKK143 pKa = 10.41FLEE146 pKa = 4.7PIIKK150 pKa = 10.35NFTATMHH157 pKa = 6.5EE158 pKa = 4.23KK159 pKa = 9.47MPNVVFCASAAGFNIKK175 pKa = 9.48NHH177 pKa = 6.76FGRR180 pKa = 11.84FYY182 pKa = 10.34PINAFANIHH191 pKa = 6.61DD192 pKa = 4.02SAARR196 pKa = 11.84LDD198 pKa = 4.44SRR200 pKa = 11.84TKK202 pKa = 10.41PNNASATILLQRR214 pKa = 11.84IFTITDD220 pKa = 3.53FHH222 pKa = 7.59SAGNDD227 pKa = 3.01NMHH230 pKa = 7.01FGLHH234 pKa = 6.0HH235 pKa = 7.04LLGAFASTTRR245 pKa = 11.84IDD247 pKa = 3.68PANATNNRR255 pKa = 11.84QFAHH259 pKa = 7.84PLRR262 pKa = 11.84QMFDD266 pKa = 3.31SLFNPVLSRR275 pKa = 11.84DD276 pKa = 3.49YY277 pKa = 10.77TRR279 pKa = 11.84RR280 pKa = 11.84STLAPLNLEE289 pKa = 4.08SPVFKK294 pKa = 10.36SYY296 pKa = 11.03RR297 pKa = 11.84LSFYY301 pKa = 11.2DD302 pKa = 4.83LVFGLTKK309 pKa = 10.82YY310 pKa = 9.84NAHH313 pKa = 7.01EE314 pKa = 4.04LTIVLQSISSNLNGAIPLSGDD335 pKa = 3.41LASMFKK341 pKa = 10.55DD342 pKa = 3.3ASGDD346 pKa = 3.32QILRR350 pKa = 11.84HH351 pKa = 6.29GYY353 pKa = 10.69AVMEE357 pKa = 4.36QPLHH361 pKa = 6.25HH362 pKa = 6.96CADD365 pKa = 3.5NMPTDD370 pKa = 3.53EE371 pKa = 4.79FDD373 pKa = 3.64VFEE376 pKa = 4.39STVTVTTRR384 pKa = 11.84HH385 pKa = 6.31PEE387 pKa = 3.55QAAADD392 pKa = 3.86HH393 pKa = 6.86KK394 pKa = 11.52YY395 pKa = 10.64LVLPNKK401 pKa = 9.68PNTGTTHH408 pKa = 6.7HH409 pKa = 6.92LASAVCDD416 pKa = 3.86TDD418 pKa = 3.49PVHH421 pKa = 6.66PVTVTQPSADD431 pKa = 3.51FVLLRR436 pKa = 11.84DD437 pKa = 3.64VTRR440 pKa = 11.84TFPIADD446 pKa = 3.24QHH448 pKa = 6.76LIPRR452 pKa = 11.84DD453 pKa = 3.95TEE455 pKa = 3.83FLLYY459 pKa = 10.91DD460 pKa = 4.13EE461 pKa = 5.97DD462 pKa = 5.58RR463 pKa = 11.84DD464 pKa = 3.84FAPTVLVLDD473 pKa = 3.88PTTNSTVSAYY483 pKa = 8.29MATLTGMVIYY493 pKa = 10.29SHH495 pKa = 7.08EE496 pKa = 4.18IDD498 pKa = 3.68ASAISFPNTEE508 pKa = 5.25DD509 pKa = 3.22INGLHH514 pKa = 6.26NCQFADD520 pKa = 3.26SATPYY525 pKa = 9.44TMVYY529 pKa = 9.85RR530 pKa = 11.84PSHH533 pKa = 5.06YY534 pKa = 9.58TLGTTAHH541 pKa = 7.63IARR544 pKa = 11.84KK545 pKa = 9.79RR546 pKa = 11.84IRR548 pKa = 11.84VKK550 pKa = 10.79DD551 pKa = 3.67NRR553 pKa = 11.84FKK555 pKa = 10.93SALYY559 pKa = 10.37LVDD562 pKa = 4.01FAKK565 pKa = 10.28IYY567 pKa = 10.09IPRR570 pKa = 11.84PILTTIDD577 pKa = 3.47TLVSHH582 pKa = 6.77GFPGFTFRR590 pKa = 11.84DD591 pKa = 3.34NVTFIGATTSALAQRR606 pKa = 11.84VVSTRR611 pKa = 11.84SNNDD615 pKa = 3.17ALPPHH620 pKa = 6.94CNFGTISLWSPFSFTPKK637 pKa = 9.97AHH639 pKa = 6.76AFWNEE644 pKa = 3.36KK645 pKa = 10.72GIFGYY650 pKa = 10.05YY651 pKa = 9.35EE652 pKa = 3.96EE653 pKa = 5.76HH654 pKa = 6.28VDD656 pKa = 4.81AYY658 pKa = 10.56FIANPRR664 pKa = 11.84TLFGTTAALVEE675 pKa = 4.53VKK677 pKa = 10.02HH678 pKa = 5.78YY679 pKa = 9.24RR680 pKa = 11.84ASMPLPP686 pKa = 3.96

Molecular weight:
76.61 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1395

686

709

697.5

80.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.527 ± 1.46

1.004 ± 0.084

6.595 ± 0.118

3.871 ± 0.813

7.527 ± 0.345

3.584 ± 0.151

4.229 ± 0.001

5.663 ± 0.366

4.229 ± 0.381

8.746 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.209

4.659 ± 0.384

6.165 ± 0.257

2.796 ± 0.302

5.376 ± 0.369

6.81 ± 0.882

7.885 ± 0.561

4.588 ± 0.81

0.717 ± 0.277

5.376 ± 0.844

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski