Rosellinia necatrix partitivirus 1-W8
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q50LH7|Q50LH7_9VIRU Putative RNA-dependent RNA polymerase OS=Rosellinia necatrix partitivirus 1-W8 OX=235994 GN=RnPV1-W8 RdRp PE=4 SV=1
MM1 pKa = 7.34 VLTIIRR7 pKa = 11.84 DD8 pKa = 3.96 YY9 pKa = 11.31 LHH11 pKa = 6.83 EE12 pKa = 4.47 AQLRR16 pKa = 11.84 LKK18 pKa = 10.78 KK19 pKa = 9.93 EE20 pKa = 3.53 WQTFQKK26 pKa = 10.51 SDD28 pKa = 3.56 QEE30 pKa = 4.22 SGYY33 pKa = 10.67 SDD35 pKa = 4.73 KK36 pKa = 11.48 LPTDD40 pKa = 3.48 YY41 pKa = 11.28 DD42 pKa = 3.29 LRR44 pKa = 11.84 RR45 pKa = 11.84 YY46 pKa = 9.56 YY47 pKa = 11.05 DD48 pKa = 3.05 SARR51 pKa = 11.84 DD52 pKa = 3.49 YY53 pKa = 11.28 DD54 pKa = 4.02 AEE56 pKa = 4.03 KK57 pKa = 10.76 HH58 pKa = 4.73 KK59 pKa = 9.98 TEE61 pKa = 4.36 EE62 pKa = 4.19 YY63 pKa = 9.47 QHH65 pKa = 6.39 NFALTHH71 pKa = 5.53 EE72 pKa = 5.67 RR73 pKa = 11.84 YY74 pKa = 7.78 TQMNADD80 pKa = 3.74 RR81 pKa = 11.84 NEE83 pKa = 3.72 PFEE86 pKa = 4.16 FYY88 pKa = 10.62 RR89 pKa = 11.84 PLEE92 pKa = 4.55 DD93 pKa = 5.31 NEE95 pKa = 4.58 LPDD98 pKa = 4.08 IRR100 pKa = 11.84 FPAPGITVLPFRR112 pKa = 11.84 YY113 pKa = 8.4 HH114 pKa = 6.28 TGQIVEE120 pKa = 4.6 TTDD123 pKa = 3.36 EE124 pKa = 4.64 LPDD127 pKa = 3.61 SGFSLHH133 pKa = 6.89 PLIDD137 pKa = 3.89 YY138 pKa = 7.55 LTKK141 pKa = 10.54 TKK143 pKa = 9.2 WLHH146 pKa = 4.22 YY147 pKa = 9.92 RR148 pKa = 11.84 PYY150 pKa = 9.33 IDD152 pKa = 4.32 KK153 pKa = 10.25 YY154 pKa = 10.58 CRR156 pKa = 11.84 PLGTTNATFSDD167 pKa = 4.5 FNRR170 pKa = 11.84 EE171 pKa = 4.17 QIPSAPIDD179 pKa = 3.58 EE180 pKa = 4.57 TRR182 pKa = 11.84 KK183 pKa = 10.54 NMVLPLVIYY192 pKa = 8.67 FLNALPFLPIHH203 pKa = 6.13 FVDD206 pKa = 3.87 TRR208 pKa = 11.84 FCGTPKK214 pKa = 9.21 HH215 pKa = 5.49 TATGYY220 pKa = 7.82 FQRR223 pKa = 11.84 FSTFFRR229 pKa = 11.84 THH231 pKa = 6.45 AYY233 pKa = 8.47 YY234 pKa = 10.86 ARR236 pKa = 11.84 NKK238 pKa = 10.3 LYY240 pKa = 10.82 ALRR243 pKa = 11.84 PTSKK247 pKa = 10.62 GYY249 pKa = 10.42 FFNTVYY255 pKa = 10.41 EE256 pKa = 5.0 FSRR259 pKa = 11.84 TWMHH263 pKa = 6.86 HH264 pKa = 5.15 IKK266 pKa = 10.1 EE267 pKa = 4.11 HH268 pKa = 6.63 GYY270 pKa = 9.81 PFVPSHH276 pKa = 7.33 DD277 pKa = 3.92 ALDD280 pKa = 3.48 NARR283 pKa = 11.84 QYY285 pKa = 11.11 RR286 pKa = 11.84 IFMQKK291 pKa = 9.53 HH292 pKa = 3.35 VTMLFTRR299 pKa = 11.84 NHH301 pKa = 5.6 ISDD304 pKa = 3.41 RR305 pKa = 11.84 DD306 pKa = 3.63 GFLKK310 pKa = 10.19 QRR312 pKa = 11.84 PVYY315 pKa = 10.56 AVDD318 pKa = 5.14 DD319 pKa = 4.05 FFILCEE325 pKa = 3.98 LMISFPLHH333 pKa = 5.26 VMARR337 pKa = 11.84 YY338 pKa = 8.26 PINGIKK344 pKa = 9.75 SCIMYY349 pKa = 10.29 SFEE352 pKa = 4.6 TIRR355 pKa = 11.84 GSNRR359 pKa = 11.84 YY360 pKa = 8.76 LDD362 pKa = 4.17 SIARR366 pKa = 11.84 DD367 pKa = 4.32 FISFFTIDD375 pKa = 3.27 WSSFDD380 pKa = 3.25 QRR382 pKa = 11.84 VPRR385 pKa = 11.84 VITDD389 pKa = 3.53 IFWTDD394 pKa = 3.68 FLRR397 pKa = 11.84 QLIVINHH404 pKa = 7.12 GYY406 pKa = 9.18 QPTYY410 pKa = 10.22 EE411 pKa = 4.15 YY412 pKa = 10.0 PAYY415 pKa = 9.4 PDD417 pKa = 4.18 LSEE420 pKa = 4.84 HH421 pKa = 7.21 DD422 pKa = 3.85 LYY424 pKa = 11.69 KK425 pKa = 10.87 RR426 pKa = 11.84 MNNLLHH432 pKa = 7.08 FLHH435 pKa = 6.25 TWYY438 pKa = 11.15 NNMVFVTADD447 pKa = 3.04 GFAYY451 pKa = 10.19 LRR453 pKa = 11.84 SAAGVPSGLLNTQYY467 pKa = 11.47 LDD469 pKa = 3.57 SFCNLFLIIDD479 pKa = 3.89 GLFEE483 pKa = 4.64 FGFTQAEE490 pKa = 4.07 ILSIVFFIMGDD501 pKa = 3.72 DD502 pKa = 3.58 NSGFTMMDD510 pKa = 3.42 IEE512 pKa = 5.1 RR513 pKa = 11.84 LTQFIEE519 pKa = 4.52 FFEE522 pKa = 4.57 SYY524 pKa = 10.53 ALKK527 pKa = 10.14 RR528 pKa = 11.84 YY529 pKa = 10.52 NMVLSKK535 pKa = 9.11 TKK537 pKa = 10.71 SVITTLRR544 pKa = 11.84 SRR546 pKa = 11.84 IEE548 pKa = 3.99 TLSYY552 pKa = 10.03 QCNGGNPKK560 pKa = 10.24 RR561 pKa = 11.84 PLGKK565 pKa = 10.38 LIAQLCYY572 pKa = 9.87 PEE574 pKa = 5.68 HH575 pKa = 6.29 GPKK578 pKa = 10.01 DD579 pKa = 3.65 KK580 pKa = 11.28 YY581 pKa = 10.25 MSARR585 pKa = 11.84 AIGIAYY591 pKa = 8.94 AAAAMDD597 pKa = 3.93 EE598 pKa = 4.46 EE599 pKa = 4.37 FHH601 pKa = 6.37 EE602 pKa = 5.0 FCRR605 pKa = 11.84 DD606 pKa = 2.95 IYY608 pKa = 10.72 HH609 pKa = 6.68 TFLPYY614 pKa = 10.13 AAPIDD619 pKa = 3.91 EE620 pKa = 5.12 HH621 pKa = 5.82 TLSMATKK628 pKa = 9.94 HH629 pKa = 5.69 LPGYY633 pKa = 10.69 FKK635 pKa = 10.56 MLDD638 pKa = 3.7 NIASEE643 pKa = 4.35 IKK645 pKa = 10.13 FDD647 pKa = 4.07 SFPTLEE653 pKa = 4.26 MVQDD657 pKa = 4.38 KK658 pKa = 10.37 YY659 pKa = 11.7 SRR661 pKa = 11.84 WQGYY665 pKa = 9.52 LSHH668 pKa = 6.66 KK669 pKa = 9.6 PKK671 pKa = 10.51 WNDD674 pKa = 2.64 AHH676 pKa = 6.93 FKK678 pKa = 10.16 FLPEE682 pKa = 3.98 TVPNNIKK689 pKa = 9.76 TMTDD693 pKa = 3.15 YY694 pKa = 11.13 QLEE697 pKa = 4.57 HH698 pKa = 7.24 KK699 pKa = 10.49 LDD701 pKa = 3.74 TPVPHH706 pKa = 7.14 SLFF709 pKa = 3.96
Molecular weight: 83.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.033
IPC2_protein 6.02
IPC_protein 6.122
Toseland 6.59
ProMoST 6.517
Dawson 6.453
Bjellqvist 6.44
Wikipedia 6.491
Rodwell 6.453
Grimsley 6.781
Solomon 6.453
Lehninger 6.453
Nozaki 6.737
DTASelect 6.927
Thurlkill 6.956
EMBOSS 6.927
Sillero 6.839
Patrickios 3.745
IPC_peptide 6.478
IPC2_peptide 6.781
IPC2.peptide.svr19 6.762
Protein with the highest isoelectric point:
>tr|Q50LH7|Q50LH7_9VIRU Putative RNA-dependent RNA polymerase OS=Rosellinia necatrix partitivirus 1-W8 OX=235994 GN=RnPV1-W8 RdRp PE=4 SV=1
MM1 pKa = 7.7 SSSRR5 pKa = 11.84 YY6 pKa = 8.7 NAAARR11 pKa = 11.84 AATIAQKK18 pKa = 10.68 SSSEE22 pKa = 3.92 APKK25 pKa = 10.29 EE26 pKa = 4.24 SKK28 pKa = 11.08 VPDD31 pKa = 3.5 NSVDD35 pKa = 3.84 FLTSYY40 pKa = 10.59 KK41 pKa = 10.18 AASSARR47 pKa = 11.84 LSTANLFTVDD57 pKa = 3.9 VLPDD61 pKa = 3.62 FTPILMFIMFHH72 pKa = 7.03 AIQLSSDD79 pKa = 2.73 IDD81 pKa = 3.64 NRR83 pKa = 11.84 KK84 pKa = 8.73 HH85 pKa = 6.2 PKK87 pKa = 8.08 ITTPTFVVYY96 pKa = 10.35 CLSLVYY102 pKa = 10.25 GYY104 pKa = 11.07 FLLSDD109 pKa = 3.74 MYY111 pKa = 11.17 VRR113 pKa = 11.84 PSASAPAQDD122 pKa = 5.69 YY123 pKa = 10.81 FDD125 pKa = 3.79 QPYY128 pKa = 10.25 KK129 pKa = 10.83 KK130 pKa = 10.05 QFSDD134 pKa = 3.55 MLLGLYY140 pKa = 9.4 VPKK143 pKa = 10.41 FLEE146 pKa = 4.7 PIIKK150 pKa = 10.35 NFTATMHH157 pKa = 6.5 EE158 pKa = 4.23 KK159 pKa = 9.47 MPNVVFCASAAGFNIKK175 pKa = 9.48 NHH177 pKa = 6.76 FGRR180 pKa = 11.84 FYY182 pKa = 10.34 PINAFANIHH191 pKa = 6.61 DD192 pKa = 4.02 SAARR196 pKa = 11.84 LDD198 pKa = 4.44 SRR200 pKa = 11.84 TKK202 pKa = 10.41 PNNASATILLQRR214 pKa = 11.84 IFTITDD220 pKa = 3.53 FHH222 pKa = 7.59 SAGNDD227 pKa = 3.01 NMHH230 pKa = 7.01 FGLHH234 pKa = 6.0 HH235 pKa = 7.04 LLGAFASTTRR245 pKa = 11.84 IDD247 pKa = 3.68 PANATNNRR255 pKa = 11.84 QFAHH259 pKa = 7.84 PLRR262 pKa = 11.84 QMFDD266 pKa = 3.31 SLFNPVLSRR275 pKa = 11.84 DD276 pKa = 3.49 YY277 pKa = 10.77 TRR279 pKa = 11.84 RR280 pKa = 11.84 STLAPLNLEE289 pKa = 4.08 SPVFKK294 pKa = 10.36 SYY296 pKa = 11.03 RR297 pKa = 11.84 LSFYY301 pKa = 11.2 DD302 pKa = 4.83 LVFGLTKK309 pKa = 10.82 YY310 pKa = 9.84 NAHH313 pKa = 7.01 EE314 pKa = 4.04 LTIVLQSISSNLNGAIPLSGDD335 pKa = 3.41 LASMFKK341 pKa = 10.55 DD342 pKa = 3.3 ASGDD346 pKa = 3.32 QILRR350 pKa = 11.84 HH351 pKa = 6.29 GYY353 pKa = 10.69 AVMEE357 pKa = 4.36 QPLHH361 pKa = 6.25 HH362 pKa = 6.96 CADD365 pKa = 3.5 NMPTDD370 pKa = 3.53 EE371 pKa = 4.79 FDD373 pKa = 3.64 VFEE376 pKa = 4.39 STVTVTTRR384 pKa = 11.84 HH385 pKa = 6.31 PEE387 pKa = 3.55 QAAADD392 pKa = 3.86 HH393 pKa = 6.86 KK394 pKa = 11.52 YY395 pKa = 10.64 LVLPNKK401 pKa = 9.68 PNTGTTHH408 pKa = 6.7 HH409 pKa = 6.92 LASAVCDD416 pKa = 3.86 TDD418 pKa = 3.49 PVHH421 pKa = 6.66 PVTVTQPSADD431 pKa = 3.51 FVLLRR436 pKa = 11.84 DD437 pKa = 3.64 VTRR440 pKa = 11.84 TFPIADD446 pKa = 3.24 QHH448 pKa = 6.76 LIPRR452 pKa = 11.84 DD453 pKa = 3.95 TEE455 pKa = 3.83 FLLYY459 pKa = 10.91 DD460 pKa = 4.13 EE461 pKa = 5.97 DD462 pKa = 5.58 RR463 pKa = 11.84 DD464 pKa = 3.84 FAPTVLVLDD473 pKa = 3.88 PTTNSTVSAYY483 pKa = 8.29 MATLTGMVIYY493 pKa = 10.29 SHH495 pKa = 7.08 EE496 pKa = 4.18 IDD498 pKa = 3.68 ASAISFPNTEE508 pKa = 5.25 DD509 pKa = 3.22 INGLHH514 pKa = 6.26 NCQFADD520 pKa = 3.26 SATPYY525 pKa = 9.44 TMVYY529 pKa = 9.85 RR530 pKa = 11.84 PSHH533 pKa = 5.06 YY534 pKa = 9.58 TLGTTAHH541 pKa = 7.63 IARR544 pKa = 11.84 KK545 pKa = 9.79 RR546 pKa = 11.84 IRR548 pKa = 11.84 VKK550 pKa = 10.79 DD551 pKa = 3.67 NRR553 pKa = 11.84 FKK555 pKa = 10.93 SALYY559 pKa = 10.37 LVDD562 pKa = 4.01 FAKK565 pKa = 10.28 IYY567 pKa = 10.09 IPRR570 pKa = 11.84 PILTTIDD577 pKa = 3.47 TLVSHH582 pKa = 6.77 GFPGFTFRR590 pKa = 11.84 DD591 pKa = 3.34 NVTFIGATTSALAQRR606 pKa = 11.84 VVSTRR611 pKa = 11.84 SNNDD615 pKa = 3.17 ALPPHH620 pKa = 6.94 CNFGTISLWSPFSFTPKK637 pKa = 9.97 AHH639 pKa = 6.76 AFWNEE644 pKa = 3.36 KK645 pKa = 10.72 GIFGYY650 pKa = 10.05 YY651 pKa = 9.35 EE652 pKa = 3.96 EE653 pKa = 5.76 HH654 pKa = 6.28 VDD656 pKa = 4.81 AYY658 pKa = 10.56 FIANPRR664 pKa = 11.84 TLFGTTAALVEE675 pKa = 4.53 VKK677 pKa = 10.02 HH678 pKa = 5.78 YY679 pKa = 9.24 RR680 pKa = 11.84 ASMPLPP686 pKa = 3.96
Molecular weight: 76.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.37
IPC2_protein 6.275
IPC_protein 6.415
Toseland 6.839
ProMoST 6.854
Dawson 6.781
Bjellqvist 6.751
Wikipedia 6.81
Rodwell 6.781
Grimsley 7.029
Solomon 6.781
Lehninger 6.781
Nozaki 7.059
DTASelect 7.234
Thurlkill 7.278
EMBOSS 7.249
Sillero 7.176
Patrickios 4.024
IPC_peptide 6.795
IPC2_peptide 7.029
IPC2.peptide.svr19 6.93
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1395
686
709
697.5
80.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.527 ± 1.46
1.004 ± 0.084
6.595 ± 0.118
3.871 ± 0.813
7.527 ± 0.345
3.584 ± 0.151
4.229 ± 0.001
5.663 ± 0.366
4.229 ± 0.381
8.746 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.652 ± 0.209
4.659 ± 0.384
6.165 ± 0.257
2.796 ± 0.302
5.376 ± 0.369
6.81 ± 0.882
7.885 ± 0.561
4.588 ± 0.81
0.717 ± 0.277
5.376 ± 0.844
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here