Escherichia phage mEpX1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hendrixvirinae; Cuauhtlivirus; Escherichia virus mEpX1

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PLU7|K7PLU7_9CAUD Late gene regulator Q OS=Escherichia phage mEpX1 OX=1147153 GN=mEpX1_057 PE=3 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.4PNRR6 pKa = 11.84SYY8 pKa = 10.61EE9 pKa = 3.97QEE11 pKa = 4.12SVEE14 pKa = 5.1RR15 pKa = 11.84ALTCANCGQKK25 pKa = 10.32LHH27 pKa = 5.86VLEE30 pKa = 4.31VHH32 pKa = 5.94VCEE35 pKa = 4.54HH36 pKa = 6.71CCAEE40 pKa = 4.99LMSDD44 pKa = 4.35PNSSMYY50 pKa = 10.6EE51 pKa = 3.98EE52 pKa = 5.0EE53 pKa = 4.99DD54 pKa = 4.39DD55 pKa = 4.1EE56 pKa = 4.75

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PGT4|K7PGT4_9CAUD KilA-N domain-containing protein OS=Escherichia phage mEpX1 OX=1147153 GN=mEpX1_061 PE=4 SV=1
MM1 pKa = 7.47NATIQTIPEE10 pKa = 4.26LLIQTRR16 pKa = 11.84GNQTEE21 pKa = 4.29VARR24 pKa = 11.84MLSCARR30 pKa = 11.84GTVLKK35 pKa = 10.59YY36 pKa = 10.74NRR38 pKa = 11.84DD39 pKa = 3.73SKK41 pKa = 11.2GEE43 pKa = 3.67RR44 pKa = 11.84HH45 pKa = 6.06VIVNGVLMVKK55 pKa = 9.6QGKK58 pKa = 9.51RR59 pKa = 11.84GRR61 pKa = 11.84PP62 pKa = 3.31

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12562

45

1183

190.3

21.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.489 ± 0.629

1.234 ± 0.165

5.74 ± 0.274

6.153 ± 0.302

3.335 ± 0.181

7.204 ± 0.339

1.457 ± 0.232

6.09 ± 0.29

6.114 ± 0.366

7.817 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.157

4.577 ± 0.221

3.718 ± 0.282

4.76 ± 0.346

5.859 ± 0.331

7.029 ± 0.325

5.779 ± 0.342

6.297 ± 0.269

1.696 ± 0.142

2.922 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski