Amino acid dipepetide frequency for Escherichia phage mEpX1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.031AlaAla: 10.031 ± 1.604
0.557AlaCys: 0.557 ± 0.225
6.21AlaAsp: 6.21 ± 0.78
6.767AlaGlu: 6.767 ± 0.804
3.344AlaPhe: 3.344 ± 0.502
7.245AlaGly: 7.245 ± 0.841
1.672AlaHis: 1.672 ± 0.329
7.165AlaIle: 7.165 ± 0.79
4.617AlaLys: 4.617 ± 0.617
7.085AlaLeu: 7.085 ± 0.863
3.344AlaMet: 3.344 ± 0.534
4.538AlaAsn: 4.538 ± 0.71
1.274AlaPro: 1.274 ± 0.298
4.617AlaGln: 4.617 ± 0.621
4.617AlaArg: 4.617 ± 0.641
7.006AlaSer: 7.006 ± 0.94
5.414AlaThr: 5.414 ± 0.814
5.334AlaVal: 5.334 ± 0.727
1.433AlaTrp: 1.433 ± 0.31
2.627AlaTyr: 2.627 ± 0.475
0.0AlaXaa: 0.0 ± 0.0
Cys
1.115CysAla: 1.115 ± 0.318
0.318CysCys: 0.318 ± 0.161
1.115CysAsp: 1.115 ± 0.318
0.796CysGlu: 0.796 ± 0.24
0.318CysPhe: 0.318 ± 0.184
1.274CysGly: 1.274 ± 0.396
0.398CysHis: 0.398 ± 0.224
0.557CysIle: 0.557 ± 0.198
0.955CysLys: 0.955 ± 0.282
0.796CysLeu: 0.796 ± 0.282
0.159CysMet: 0.159 ± 0.128
0.557CysAsn: 0.557 ± 0.166
0.478CysPro: 0.478 ± 0.204
0.318CysGln: 0.318 ± 0.166
0.876CysArg: 0.876 ± 0.322
0.478CysSer: 0.478 ± 0.191
0.796CysThr: 0.796 ± 0.241
0.796CysVal: 0.796 ± 0.243
0.239CysTrp: 0.239 ± 0.194
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.493AspAla: 5.493 ± 0.72
0.637AspCys: 0.637 ± 0.281
3.981AspAsp: 3.981 ± 0.526
3.662AspGlu: 3.662 ± 0.543
2.309AspPhe: 2.309 ± 0.495
4.617AspGly: 4.617 ± 0.728
0.478AspHis: 0.478 ± 0.229
3.025AspIle: 3.025 ± 0.463
3.264AspLys: 3.264 ± 0.537
5.095AspLeu: 5.095 ± 0.656
1.513AspMet: 1.513 ± 0.308
2.866AspAsn: 2.866 ± 0.392
1.672AspPro: 1.672 ± 0.366
2.15AspGln: 2.15 ± 0.371
3.344AspArg: 3.344 ± 0.63
3.821AspSer: 3.821 ± 0.639
1.99AspThr: 1.99 ± 0.413
4.14AspVal: 4.14 ± 0.737
0.796AspTrp: 0.796 ± 0.239
2.548AspTyr: 2.548 ± 0.498
0.0AspXaa: 0.0 ± 0.0
Glu
4.936GluAla: 4.936 ± 0.728
0.876GluCys: 0.876 ± 0.31
2.468GluAsp: 2.468 ± 0.494
4.06GluGlu: 4.06 ± 0.722
1.99GluPhe: 1.99 ± 0.383
3.821GluGly: 3.821 ± 0.588
0.876GluHis: 0.876 ± 0.294
4.06GluIle: 4.06 ± 0.435
4.379GluLys: 4.379 ± 0.551
5.414GluLeu: 5.414 ± 0.596
1.831GluMet: 1.831 ± 0.383
3.025GluAsn: 3.025 ± 0.443
2.388GluPro: 2.388 ± 0.456
4.219GluGln: 4.219 ± 0.512
3.821GluArg: 3.821 ± 0.664
3.821GluSer: 3.821 ± 0.585
2.946GluThr: 2.946 ± 0.613
3.662GluVal: 3.662 ± 0.552
0.796GluTrp: 0.796 ± 0.274
2.15GluTyr: 2.15 ± 0.503
0.0GluXaa: 0.0 ± 0.0
Phe
3.264PheAla: 3.264 ± 0.519
0.637PheCys: 0.637 ± 0.223
1.99PheAsp: 1.99 ± 0.377
1.831PheGlu: 1.831 ± 0.345
0.637PhePhe: 0.637 ± 0.235
2.786PheGly: 2.786 ± 0.415
0.557PheHis: 0.557 ± 0.205
2.229PheIle: 2.229 ± 0.492
1.99PheLys: 1.99 ± 0.42
1.433PheLeu: 1.433 ± 0.367
0.796PheMet: 0.796 ± 0.274
1.672PheAsn: 1.672 ± 0.315
1.353PhePro: 1.353 ± 0.343
0.876PheGln: 0.876 ± 0.248
1.751PheArg: 1.751 ± 0.458
3.105PheSer: 3.105 ± 0.4
3.105PheThr: 3.105 ± 0.485
1.831PheVal: 1.831 ± 0.387
0.557PheTrp: 0.557 ± 0.181
0.955PheTyr: 0.955 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
5.732GlyAla: 5.732 ± 1.006
1.433GlyCys: 1.433 ± 0.402
4.06GlyAsp: 4.06 ± 0.626
4.219GlyGlu: 4.219 ± 0.548
2.786GlyPhe: 2.786 ± 0.521
5.095GlyGly: 5.095 ± 0.66
1.035GlyHis: 1.035 ± 0.275
3.981GlyIle: 3.981 ± 0.594
4.856GlyLys: 4.856 ± 0.602
6.13GlyLeu: 6.13 ± 0.683
2.866GlyMet: 2.866 ± 0.397
4.14GlyAsn: 4.14 ± 0.621
1.592GlyPro: 1.592 ± 0.328
3.184GlyGln: 3.184 ± 0.461
4.219GlyArg: 4.219 ± 0.636
3.264GlySer: 3.264 ± 0.569
5.095GlyThr: 5.095 ± 0.866
4.777GlyVal: 4.777 ± 0.66
1.035GlyTrp: 1.035 ± 0.225
2.548GlyTyr: 2.548 ± 0.397
0.0GlyXaa: 0.0 ± 0.0
His
1.274HisAla: 1.274 ± 0.406
0.159HisCys: 0.159 ± 0.096
0.637HisAsp: 0.637 ± 0.196
0.717HisGlu: 0.717 ± 0.286
0.159HisPhe: 0.159 ± 0.119
1.035HisGly: 1.035 ± 0.269
0.398HisHis: 0.398 ± 0.203
1.035HisIle: 1.035 ± 0.316
1.035HisLys: 1.035 ± 0.342
1.513HisLeu: 1.513 ± 0.424
0.159HisMet: 0.159 ± 0.109
0.478HisAsn: 0.478 ± 0.205
0.796HisPro: 0.796 ± 0.284
0.876HisGln: 0.876 ± 0.308
0.955HisArg: 0.955 ± 0.264
1.194HisSer: 1.194 ± 0.411
0.478HisThr: 0.478 ± 0.178
0.717HisVal: 0.717 ± 0.259
0.478HisTrp: 0.478 ± 0.184
0.478HisTyr: 0.478 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
6.369IleAla: 6.369 ± 0.578
0.557IleCys: 0.557 ± 0.229
5.016IleAsp: 5.016 ± 0.532
3.264IleGlu: 3.264 ± 0.459
2.229IlePhe: 2.229 ± 0.429
3.901IleGly: 3.901 ± 0.599
1.035IleHis: 1.035 ± 0.259
3.901IleIle: 3.901 ± 0.506
3.025IleLys: 3.025 ± 0.523
4.14IleLeu: 4.14 ± 0.658
1.194IleMet: 1.194 ± 0.326
3.821IleAsn: 3.821 ± 0.464
2.388IlePro: 2.388 ± 0.459
2.707IleGln: 2.707 ± 0.616
3.264IleArg: 3.264 ± 0.566
5.175IleSer: 5.175 ± 0.706
3.423IleThr: 3.423 ± 0.429
3.264IleVal: 3.264 ± 0.674
0.796IleTrp: 0.796 ± 0.237
1.433IleTyr: 1.433 ± 0.275
0.0IleXaa: 0.0 ± 0.0
Lys
5.652LysAla: 5.652 ± 0.65
0.478LysCys: 0.478 ± 0.231
3.264LysAsp: 3.264 ± 0.55
3.742LysGlu: 3.742 ± 0.638
1.513LysPhe: 1.513 ± 0.391
3.503LysGly: 3.503 ± 0.401
0.796LysHis: 0.796 ± 0.319
3.981LysIle: 3.981 ± 0.746
3.423LysLys: 3.423 ± 0.685
5.254LysLeu: 5.254 ± 0.592
1.672LysMet: 1.672 ± 0.381
2.309LysAsn: 2.309 ± 0.337
2.707LysPro: 2.707 ± 0.527
3.742LysGln: 3.742 ± 0.688
3.583LysArg: 3.583 ± 0.657
4.14LysSer: 4.14 ± 0.512
4.06LysThr: 4.06 ± 0.523
3.981LysVal: 3.981 ± 0.527
1.115LysTrp: 1.115 ± 0.247
2.15LysTyr: 2.15 ± 0.41
0.0LysXaa: 0.0 ± 0.0
Leu
7.324LeuAla: 7.324 ± 0.879
1.353LeuCys: 1.353 ± 0.359
4.697LeuAsp: 4.697 ± 0.548
5.652LeuGlu: 5.652 ± 0.724
1.751LeuPhe: 1.751 ± 0.31
3.821LeuGly: 3.821 ± 0.623
0.796LeuHis: 0.796 ± 0.292
5.016LeuIle: 5.016 ± 0.693
5.334LeuLys: 5.334 ± 0.675
6.289LeuLeu: 6.289 ± 0.812
1.513LeuMet: 1.513 ± 0.374
3.901LeuAsn: 3.901 ± 0.583
3.344LeuPro: 3.344 ± 0.564
3.184LeuGln: 3.184 ± 0.487
5.334LeuArg: 5.334 ± 0.573
6.449LeuSer: 6.449 ± 0.676
4.777LeuThr: 4.777 ± 0.63
5.016LeuVal: 5.016 ± 0.615
0.637LeuTrp: 0.637 ± 0.25
1.99LeuTyr: 1.99 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
3.742MetAla: 3.742 ± 0.617
0.557MetCys: 0.557 ± 0.213
1.831MetAsp: 1.831 ± 0.382
1.115MetGlu: 1.115 ± 0.292
0.796MetPhe: 0.796 ± 0.271
1.592MetGly: 1.592 ± 0.272
0.318MetHis: 0.318 ± 0.148
1.115MetIle: 1.115 ± 0.31
2.229MetLys: 2.229 ± 0.356
2.15MetLeu: 2.15 ± 0.356
0.717MetMet: 0.717 ± 0.273
0.955MetAsn: 0.955 ± 0.332
1.353MetPro: 1.353 ± 0.326
1.433MetGln: 1.433 ± 0.268
1.592MetArg: 1.592 ± 0.362
2.309MetSer: 2.309 ± 0.453
1.513MetThr: 1.513 ± 0.362
1.115MetVal: 1.115 ± 0.264
0.318MetTrp: 0.318 ± 0.159
0.557MetTyr: 0.557 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
4.617AsnAla: 4.617 ± 0.833
0.398AsnCys: 0.398 ± 0.185
2.627AsnAsp: 2.627 ± 0.327
2.468AsnGlu: 2.468 ± 0.461
1.99AsnPhe: 1.99 ± 0.445
4.777AsnGly: 4.777 ± 0.782
1.035AsnHis: 1.035 ± 0.334
3.184AsnIle: 3.184 ± 0.425
2.548AsnLys: 2.548 ± 0.459
2.946AsnLeu: 2.946 ± 0.491
0.876AsnMet: 0.876 ± 0.229
1.751AsnAsn: 1.751 ± 0.408
2.786AsnPro: 2.786 ± 0.597
2.229AsnGln: 2.229 ± 0.371
1.513AsnArg: 1.513 ± 0.319
2.309AsnSer: 2.309 ± 0.451
3.025AsnThr: 3.025 ± 0.466
1.99AsnVal: 1.99 ± 0.548
0.796AsnTrp: 0.796 ± 0.217
1.911AsnTyr: 1.911 ± 0.465
0.0AsnXaa: 0.0 ± 0.0
Pro
3.583ProAla: 3.583 ± 0.626
0.239ProCys: 0.239 ± 0.14
1.751ProAsp: 1.751 ± 0.408
2.388ProGlu: 2.388 ± 0.465
1.513ProPhe: 1.513 ± 0.362
3.423ProGly: 3.423 ± 0.441
0.637ProHis: 0.637 ± 0.203
1.513ProIle: 1.513 ± 0.435
2.07ProLys: 2.07 ± 0.395
2.707ProLeu: 2.707 ± 0.628
1.672ProMet: 1.672 ± 0.371
1.115ProAsn: 1.115 ± 0.293
1.99ProPro: 1.99 ± 0.352
1.751ProGln: 1.751 ± 0.401
1.911ProArg: 1.911 ± 0.382
2.946ProSer: 2.946 ± 0.582
1.353ProThr: 1.353 ± 0.376
3.184ProVal: 3.184 ± 0.51
0.478ProTrp: 0.478 ± 0.253
0.955ProTyr: 0.955 ± 0.257
0.0ProXaa: 0.0 ± 0.0
Gln
5.095GlnAla: 5.095 ± 0.883
0.478GlnCys: 0.478 ± 0.19
1.115GlnAsp: 1.115 ± 0.315
2.309GlnGlu: 2.309 ± 0.325
1.751GlnPhe: 1.751 ± 0.317
2.309GlnGly: 2.309 ± 0.569
0.478GlnHis: 0.478 ± 0.194
2.468GlnIle: 2.468 ± 0.363
3.344GlnLys: 3.344 ± 0.403
4.299GlnLeu: 4.299 ± 0.651
1.274GlnMet: 1.274 ± 0.293
2.786GlnAsn: 2.786 ± 0.417
1.672GlnPro: 1.672 ± 0.406
3.025GlnGln: 3.025 ± 0.556
3.344GlnArg: 3.344 ± 0.571
3.264GlnSer: 3.264 ± 0.574
3.184GlnThr: 3.184 ± 0.704
3.264GlnVal: 3.264 ± 0.488
0.955GlnTrp: 0.955 ± 0.336
1.194GlnTyr: 1.194 ± 0.369
0.0GlnXaa: 0.0 ± 0.0
Arg
4.777ArgAla: 4.777 ± 0.784
0.637ArgCys: 0.637 ± 0.273
3.423ArgAsp: 3.423 ± 0.598
3.583ArgGlu: 3.583 ± 0.674
2.07ArgPhe: 2.07 ± 0.44
3.503ArgGly: 3.503 ± 0.505
1.194ArgHis: 1.194 ± 0.266
3.821ArgIle: 3.821 ± 0.592
4.936ArgLys: 4.936 ± 0.616
4.856ArgLeu: 4.856 ± 0.472
1.672ArgMet: 1.672 ± 0.384
2.627ArgAsn: 2.627 ± 0.428
1.194ArgPro: 1.194 ± 0.351
3.344ArgGln: 3.344 ± 0.525
3.344ArgArg: 3.344 ± 0.6
3.184ArgSer: 3.184 ± 0.551
3.184ArgThr: 3.184 ± 0.459
3.662ArgVal: 3.662 ± 0.653
1.274ArgTrp: 1.274 ± 0.308
2.309ArgTyr: 2.309 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
6.687SerAla: 6.687 ± 0.931
0.478SerCys: 0.478 ± 0.19
4.14SerAsp: 4.14 ± 0.47
4.777SerGlu: 4.777 ± 0.598
2.07SerPhe: 2.07 ± 0.339
5.493SerGly: 5.493 ± 0.706
0.876SerHis: 0.876 ± 0.31
3.423SerIle: 3.423 ± 0.461
3.742SerLys: 3.742 ± 0.879
5.812SerLeu: 5.812 ± 0.653
1.831SerMet: 1.831 ± 0.369
2.707SerAsn: 2.707 ± 0.445
2.866SerPro: 2.866 ± 0.53
3.742SerGln: 3.742 ± 0.848
4.856SerArg: 4.856 ± 0.574
4.617SerSer: 4.617 ± 0.83
3.742SerThr: 3.742 ± 0.642
5.334SerVal: 5.334 ± 0.783
1.592SerTrp: 1.592 ± 0.291
1.513SerTyr: 1.513 ± 0.443
0.0SerXaa: 0.0 ± 0.0
Thr
6.05ThrAla: 6.05 ± 0.822
0.717ThrCys: 0.717 ± 0.219
3.184ThrAsp: 3.184 ± 0.466
3.025ThrGlu: 3.025 ± 0.543
2.946ThrPhe: 2.946 ± 0.501
5.493ThrGly: 5.493 ± 0.689
0.717ThrHis: 0.717 ± 0.207
3.901ThrIle: 3.901 ± 0.632
2.548ThrLys: 2.548 ± 0.418
3.503ThrLeu: 3.503 ± 0.525
1.353ThrMet: 1.353 ± 0.319
2.07ThrAsn: 2.07 ± 0.486
2.707ThrPro: 2.707 ± 0.624
2.15ThrGln: 2.15 ± 0.518
2.866ThrArg: 2.866 ± 0.404
4.458ThrSer: 4.458 ± 0.63
2.786ThrThr: 2.786 ± 0.517
4.219ThrVal: 4.219 ± 0.541
1.513ThrTrp: 1.513 ± 0.284
1.592ThrTyr: 1.592 ± 0.404
0.0ThrXaa: 0.0 ± 0.0
Val
5.175ValAla: 5.175 ± 0.745
0.717ValCys: 0.717 ± 0.235
3.264ValAsp: 3.264 ± 0.472
3.981ValGlu: 3.981 ± 0.407
1.911ValPhe: 1.911 ± 0.323
4.617ValGly: 4.617 ± 0.69
0.796ValHis: 0.796 ± 0.236
3.742ValIle: 3.742 ± 0.676
4.617ValLys: 4.617 ± 0.671
5.334ValLeu: 5.334 ± 0.711
1.751ValMet: 1.751 ± 0.323
3.025ValAsn: 3.025 ± 0.627
2.627ValPro: 2.627 ± 0.475
1.751ValGln: 1.751 ± 0.309
3.423ValArg: 3.423 ± 0.433
5.652ValSer: 5.652 ± 0.834
4.06ValThr: 4.06 ± 0.609
4.14ValVal: 4.14 ± 0.635
1.115ValTrp: 1.115 ± 0.279
1.274ValTyr: 1.274 ± 0.306
0.0ValXaa: 0.0 ± 0.0
Trp
1.035TrpAla: 1.035 ± 0.245
0.637TrpCys: 0.637 ± 0.235
1.115TrpAsp: 1.115 ± 0.275
1.115TrpGlu: 1.115 ± 0.298
0.557TrpPhe: 0.557 ± 0.223
1.194TrpGly: 1.194 ± 0.323
0.398TrpHis: 0.398 ± 0.158
0.796TrpIle: 0.796 ± 0.257
0.717TrpLys: 0.717 ± 0.203
1.513TrpLeu: 1.513 ± 0.462
0.398TrpMet: 0.398 ± 0.179
0.318TrpAsn: 0.318 ± 0.142
0.557TrpPro: 0.557 ± 0.196
0.876TrpGln: 0.876 ± 0.238
1.353TrpArg: 1.353 ± 0.317
1.194TrpSer: 1.194 ± 0.317
1.115TrpThr: 1.115 ± 0.416
1.194TrpVal: 1.194 ± 0.246
0.398TrpTrp: 0.398 ± 0.172
0.478TrpTyr: 0.478 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.946TyrAla: 2.946 ± 0.476
0.478TyrCys: 0.478 ± 0.21
1.513TyrAsp: 1.513 ± 0.292
2.07TyrGlu: 2.07 ± 0.509
0.717TyrPhe: 0.717 ± 0.3
2.548TyrGly: 2.548 ± 0.428
0.08TyrHis: 0.08 ± 0.076
1.99TyrIle: 1.99 ± 0.381
1.513TyrLys: 1.513 ± 0.383
1.911TyrLeu: 1.911 ± 0.313
0.637TyrMet: 0.637 ± 0.217
1.115TyrAsn: 1.115 ± 0.237
1.433TyrPro: 1.433 ± 0.411
1.433TyrGln: 1.433 ± 0.355
2.627TyrArg: 2.627 ± 0.463
1.911TyrSer: 1.911 ± 0.461
1.751TyrThr: 1.751 ± 0.405
1.353TyrVal: 1.353 ± 0.263
0.637TyrTrp: 0.637 ± 0.237
0.557TyrTyr: 0.557 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12562 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski