Collinsella sp. An2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; unclassified Collinsella

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2074 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y4HNC3|A0A1Y4HNC3_9ACTN Uncharacterized protein OS=Collinsella sp. An2 OX=1965585 GN=B5F33_04960 PE=4 SV=1
MM1 pKa = 7.75AFNAQQTGRR10 pKa = 11.84ARR12 pKa = 11.84IAVIIVALVAVAGIAVALVLQLATPATTGSADD44 pKa = 3.65ASAQNAAQAQSDD56 pKa = 4.35GSQDD60 pKa = 3.56AQDD63 pKa = 3.85EE64 pKa = 4.6DD65 pKa = 4.23VMEE68 pKa = 4.58TVDD71 pKa = 3.58AQYY74 pKa = 9.14GTAAQTLRR82 pKa = 11.84TQYY85 pKa = 10.58EE86 pKa = 4.12ADD88 pKa = 3.71PSNPSALLNLANGYY102 pKa = 9.81FDD104 pKa = 3.6WGVAALNHH112 pKa = 6.59SDD114 pKa = 3.34GTEE117 pKa = 4.14DD118 pKa = 3.33EE119 pKa = 4.16AHH121 pKa = 6.25ARR123 pKa = 11.84EE124 pKa = 4.34IFTNAIEE131 pKa = 4.5YY132 pKa = 10.0YY133 pKa = 10.56DD134 pKa = 4.68EE135 pKa = 4.69YY136 pKa = 11.59LDD138 pKa = 4.44GNPGSKK144 pKa = 10.05SVVVDD149 pKa = 3.6RR150 pKa = 11.84AICVFYY156 pKa = 10.72TGDD159 pKa = 3.32HH160 pKa = 6.98DD161 pKa = 4.45AAITALEE168 pKa = 4.23NLLADD173 pKa = 4.97DD174 pKa = 5.84DD175 pKa = 4.68SFAPAWANLGMFYY188 pKa = 9.72EE189 pKa = 4.6TDD191 pKa = 3.07GRR193 pKa = 11.84TDD195 pKa = 3.59DD196 pKa = 4.97AATAYY201 pKa = 9.98QRR203 pKa = 11.84AIDD206 pKa = 4.11AAGDD210 pKa = 3.5DD211 pKa = 3.68DD212 pKa = 5.24AYY214 pKa = 11.4NVKK217 pKa = 10.5DD218 pKa = 3.73YY219 pKa = 11.27AQQRR223 pKa = 11.84LDD225 pKa = 3.45ALQASEE231 pKa = 4.05

Molecular weight:
24.5 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y4HUQ8|A0A1Y4HUQ8_9ACTN Uncharacterized protein OS=Collinsella sp. An2 OX=1965585 GN=B5F33_01955 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27GGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight:
5.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2074

0

2074

704075

37

2711

339.5

36.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.663 ± 0.077

1.538 ± 0.024

6.452 ± 0.048

6.404 ± 0.05

3.516 ± 0.033

8.305 ± 0.053

2.059 ± 0.025

5.067 ± 0.038

3.189 ± 0.047

9.11 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.026

2.727 ± 0.033

4.444 ± 0.03

2.94 ± 0.026

6.365 ± 0.064

5.873 ± 0.041

5.61 ± 0.057

8.278 ± 0.038

1.041 ± 0.019

2.785 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski