Collinsella sp. An2
Average proteome isoelectric point is 5.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2074 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y4HNC3|A0A1Y4HNC3_9ACTN Uncharacterized protein OS=Collinsella sp. An2 OX=1965585 GN=B5F33_04960 PE=4 SV=1
MM1 pKa = 7.75 AFNAQQTGRR10 pKa = 11.84 ARR12 pKa = 11.84 IAVIIVALVAVAGIAVALVLQLATPATTGSADD44 pKa = 3.65 ASAQNAAQAQSDD56 pKa = 4.35 GSQDD60 pKa = 3.56 AQDD63 pKa = 3.85 EE64 pKa = 4.6 DD65 pKa = 4.23 VMEE68 pKa = 4.58 TVDD71 pKa = 3.58 AQYY74 pKa = 9.14 GTAAQTLRR82 pKa = 11.84 TQYY85 pKa = 10.58 EE86 pKa = 4.12 ADD88 pKa = 3.71 PSNPSALLNLANGYY102 pKa = 9.81 FDD104 pKa = 3.6 WGVAALNHH112 pKa = 6.59 SDD114 pKa = 3.34 GTEE117 pKa = 4.14 DD118 pKa = 3.33 EE119 pKa = 4.16 AHH121 pKa = 6.25 ARR123 pKa = 11.84 EE124 pKa = 4.34 IFTNAIEE131 pKa = 4.5 YY132 pKa = 10.0 YY133 pKa = 10.56 DD134 pKa = 4.68 EE135 pKa = 4.69 YY136 pKa = 11.59 LDD138 pKa = 4.44 GNPGSKK144 pKa = 10.05 SVVVDD149 pKa = 3.6 RR150 pKa = 11.84 AICVFYY156 pKa = 10.72 TGDD159 pKa = 3.32 HH160 pKa = 6.98 DD161 pKa = 4.45 AAITALEE168 pKa = 4.23 NLLADD173 pKa = 4.97 DD174 pKa = 5.84 DD175 pKa = 4.68 SFAPAWANLGMFYY188 pKa = 9.72 EE189 pKa = 4.6 TDD191 pKa = 3.07 GRR193 pKa = 11.84 TDD195 pKa = 3.59 DD196 pKa = 4.97 AATAYY201 pKa = 9.98 QRR203 pKa = 11.84 AIDD206 pKa = 4.11 AAGDD210 pKa = 3.5 DD211 pKa = 3.68 DD212 pKa = 5.24 AYY214 pKa = 11.4 NVKK217 pKa = 10.5 DD218 pKa = 3.73 YY219 pKa = 11.27 AQQRR223 pKa = 11.84 LDD225 pKa = 3.45 ALQASEE231 pKa = 4.05
Molecular weight: 24.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.554
EMBOSS 3.694
Sillero 3.846
Patrickios 1.125
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|A0A1Y4HUQ8|A0A1Y4HUQ8_9ACTN Uncharacterized protein OS=Collinsella sp. An2 OX=1965585 GN=B5F33_01955 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 GGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.73 GRR39 pKa = 11.84 KK40 pKa = 8.88 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2074
0
2074
704075
37
2711
339.5
36.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.663 ± 0.077
1.538 ± 0.024
6.452 ± 0.048
6.404 ± 0.05
3.516 ± 0.033
8.305 ± 0.053
2.059 ± 0.025
5.067 ± 0.038
3.189 ± 0.047
9.11 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.635 ± 0.026
2.727 ± 0.033
4.444 ± 0.03
2.94 ± 0.026
6.365 ± 0.064
5.873 ± 0.041
5.61 ± 0.057
8.278 ± 0.038
1.041 ± 0.019
2.785 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here