Tupanvirus soda lake

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; unclassified Mimiviridae; Tupanvirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1331 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N1NPQ8|A0A6N1NPQ8_9VIRU Putative orfan OS=Tupanvirus soda lake OX=2126985 PE=4 SV=1
MM1 pKa = 7.63AVTIDD6 pKa = 4.58LVFKK10 pKa = 10.7HH11 pKa = 6.37IDD13 pKa = 3.15INVVVLVGEE22 pKa = 4.38SCFVGDD28 pKa = 4.88IKK30 pKa = 11.04PDD32 pKa = 3.13FHH34 pKa = 8.45LRR36 pKa = 11.84DD37 pKa = 3.66GGVLCIEE44 pKa = 4.41TVASNVVLADD54 pKa = 4.01DD55 pKa = 5.16LDD57 pKa = 4.11VVMLFQFVDD66 pKa = 3.57DD67 pKa = 4.71LDD69 pKa = 3.99RR70 pKa = 11.84TFLTTNSDD78 pKa = 3.05IVVVGFFVVQCISDD92 pKa = 3.57GTTIEE97 pKa = 4.04VHH99 pKa = 6.51SLAEE103 pKa = 4.19CAFVTNTIRR112 pKa = 11.84EE113 pKa = 4.05QFAQCVDD120 pKa = 3.6NEE122 pKa = 4.15ILLLLAYY129 pKa = 10.07VYY131 pKa = 11.01SLLKK135 pKa = 10.8SYY137 pKa = 11.43DD138 pKa = 3.23RR139 pKa = 11.84FLL141 pKa = 5.69

Molecular weight: 15.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N1NXW7|A0A6N1NXW7_9VIRU Putative orfan OS=Tupanvirus soda lake OX=2126985 PE=4 SV=1
MM1 pKa = 7.4GKK3 pKa = 10.15VNGVTGAVTIGVVTDD18 pKa = 4.08GVVTRR23 pKa = 11.84GGVITGGGVTAGGGVTAGGVTGGGITGGGGITGGGITGGGITGGGGITGGGITGGGGITGGGITGGGGITGGGITGGGITGGGGITGGGITGGGGITGGGITGGGITGGVVIAGPHH139 pKa = 4.92NVNTLVSSTKK149 pKa = 9.16VHH151 pKa = 5.54VFFSGLTIYY160 pKa = 10.94NSVRR164 pKa = 11.84FGIVPAFLAVCTSRR178 pKa = 11.84IRR180 pKa = 11.84TQLSSKK186 pKa = 10.47KK187 pKa = 9.99LLALILISGGTLLALLVNFMPMVAALGIPLLFKK220 pKa = 10.41NVRR223 pKa = 11.84IISSLLEE230 pKa = 3.93LCAVTII236 pKa = 4.4

Molecular weight: 21.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1331

0

1331

438616

50

3054

329.5

37.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.861 ± 0.061

1.82 ± 0.047

6.264 ± 0.046

5.755 ± 0.066

4.647 ± 0.045

4.599 ± 0.106

2.098 ± 0.038

9.356 ± 0.069

8.727 ± 0.104

8.016 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.036

8.465 ± 0.084

3.732 ± 0.071

3.287 ± 0.064

3.257 ± 0.053

6.496 ± 0.069

5.668 ± 0.062

5.364 ± 0.054

0.843 ± 0.022

5.268 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski