BD1-7 clade bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Spongiibacteraceae; unclassified Spongiibacteraceae; BD1-7 clade

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4504 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S9PSZ9|A0A5S9PSZ9_9GAMM Uncharacterized protein OS=BD1-7 clade bacterium OX=2029982 GN=OPDIPICF_01203 PE=4 SV=1
MM1 pKa = 7.39TEE3 pKa = 4.17TMVHH7 pKa = 6.17PQQHH11 pKa = 6.07SKK13 pKa = 10.9CSAAMTNGIMQLSGWFASAMLVMFVCLIVSAAPAAFALTPANTLISNQATASYY66 pKa = 9.85KK67 pKa = 10.82SSDD70 pKa = 3.2GAQRR74 pKa = 11.84LATSNSVRR82 pKa = 11.84TIVTSVAAATLTQDD96 pKa = 3.04QTRR99 pKa = 11.84DD100 pKa = 3.41VLASGSVVFSHH111 pKa = 7.53IISNNGNVGDD121 pKa = 4.71SYY123 pKa = 11.46TITLTNQTGDD133 pKa = 3.71DD134 pKa = 4.1FNFTSLSAFADD145 pKa = 3.47ADD147 pKa = 4.05ANGQPDD153 pKa = 4.08TVQPLTEE160 pKa = 4.21TPVLAMQEE168 pKa = 4.12SFNIVIVGSIGTGEE182 pKa = 4.1NPGNEE187 pKa = 3.92AQVQVTATSILDD199 pKa = 3.46NSVVLNNTDD208 pKa = 3.2TAIVVDD214 pKa = 4.49DD215 pKa = 4.35AVVDD219 pKa = 3.74ITQSIDD225 pKa = 3.21LTQGIAGQTPIRR237 pKa = 11.84ITYY240 pKa = 8.58FYY242 pKa = 10.68VNSGSRR248 pKa = 11.84AATAITLGNLLPDD261 pKa = 3.73GFVYY265 pKa = 10.34QGNAVWSFGNLPLTDD280 pKa = 4.4ANEE283 pKa = 4.22EE284 pKa = 4.46TQGSAPSIIYY294 pKa = 9.72CAYY297 pKa = 10.37QPSCTQADD305 pKa = 3.79RR306 pKa = 11.84LSFVIDD312 pKa = 3.59EE313 pKa = 4.81LGAGQSGSVSFDD325 pKa = 2.84IAIDD329 pKa = 3.62ATQLPGVIQNTATYY343 pKa = 10.3DD344 pKa = 3.59YY345 pKa = 10.97LPFNGTQITGRR356 pKa = 11.84STNTVPFTVVGDD368 pKa = 3.92EE369 pKa = 4.5SLTLTDD375 pKa = 5.53DD376 pKa = 3.84SVTDD380 pKa = 4.01AVPGTTVFVTHH391 pKa = 6.65TVTNTGTGNDD401 pKa = 3.48TFNIRR406 pKa = 11.84VNQISSNFPVGTVFFLYY423 pKa = 10.48QSDD426 pKa = 3.96GLTPLNDD433 pKa = 3.76SNLDD437 pKa = 3.36GDD439 pKa = 5.55LDD441 pKa = 4.06TGPLPKK447 pKa = 10.34DD448 pKa = 3.41DD449 pKa = 5.03DD450 pKa = 4.39VNIIIGITLPPSAATGTYY468 pKa = 10.18SVGLRR473 pKa = 11.84GTSLQSNNTFADD485 pKa = 4.94AIATVSVLAPLLAVDD500 pKa = 4.49LTGIAANGDD509 pKa = 4.0SACDD513 pKa = 3.64ADD515 pKa = 4.84ADD517 pKa = 4.06NCGFEE522 pKa = 5.02AFDD525 pKa = 4.11PADD528 pKa = 3.73PAVISQTIDD537 pKa = 2.94PGQTANFVLYY547 pKa = 10.52ARR549 pKa = 11.84NLSEE553 pKa = 5.46IIDD556 pKa = 3.91LFGFSASTDD565 pKa = 3.37NTFGSQTLPAGWSISYY581 pKa = 9.41TEE583 pKa = 4.46LTDD586 pKa = 3.74EE587 pKa = 5.03VIASTGTLSPGGVKK601 pKa = 10.2AFKK604 pKa = 10.71VAVTTSEE611 pKa = 4.02TALATTEE618 pKa = 3.95DD619 pKa = 4.72LYY621 pKa = 10.97IQVLSATSLRR631 pKa = 11.84SDD633 pKa = 3.74RR634 pKa = 11.84LRR636 pKa = 11.84LSISINEE643 pKa = 4.18VQSVSLGPDD652 pKa = 2.99NASTEE657 pKa = 4.18EE658 pKa = 3.96PNQFVIYY665 pKa = 10.12RR666 pKa = 11.84HH667 pKa = 5.51EE668 pKa = 4.14LVNDD672 pKa = 3.88GNAAVTFDD680 pKa = 4.71GIAVALTTPDD690 pKa = 2.87WSIALYY696 pKa = 10.43EE697 pKa = 4.16DD698 pKa = 3.99TAGGTVDD705 pKa = 3.67EE706 pKa = 5.14WDD708 pKa = 3.88VDD710 pKa = 3.78DD711 pKa = 5.89QIINGSFSVAAFSSKK726 pKa = 9.07VVYY729 pKa = 10.33VRR731 pKa = 11.84AFIPSSATPGQVEE744 pKa = 4.52TATVTATFNAGVDD757 pKa = 3.51QVSAIDD763 pKa = 4.25VITANPFDD771 pKa = 4.02IDD773 pKa = 3.62IVKK776 pKa = 10.36AQAKK780 pKa = 9.22DD781 pKa = 3.66ANCDD785 pKa = 3.31GTEE788 pKa = 4.01DD789 pKa = 3.4SAFAVGSFGALPGEE803 pKa = 4.58CIVYY807 pKa = 10.37RR808 pKa = 11.84LRR810 pKa = 11.84VSNFSDD816 pKa = 2.82ITAVNAVIRR825 pKa = 11.84DD826 pKa = 3.74ATPEE830 pKa = 3.92YY831 pKa = 9.75TFYY834 pKa = 11.24SATQPATRR842 pKa = 11.84CTPAVCVFVSEE853 pKa = 4.62PAASQTGEE861 pKa = 4.03LEE863 pKa = 4.25INVGDD868 pKa = 3.67IDD870 pKa = 4.66AGNTVDD876 pKa = 6.01FFFSVKK882 pKa = 10.33VSDD885 pKa = 3.73TT886 pKa = 3.44

Molecular weight: 92.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S9PUD2|A0A5S9PUD2_9GAMM Bacterioferritin OS=BD1-7 clade bacterium OX=2029982 GN=bfr PE=3 SV=1
MM1 pKa = 7.06FAAITHH7 pKa = 5.55QSRR10 pKa = 11.84HH11 pKa = 5.74SGHH14 pKa = 6.18SFVPASRR21 pKa = 11.84FLALNKK27 pKa = 9.64AARR30 pKa = 11.84VGTRR34 pKa = 11.84LNKK37 pKa = 9.97KK38 pKa = 8.61PALASMHH45 pKa = 6.32VSDD48 pKa = 5.48SYY50 pKa = 11.65CADD53 pKa = 3.29ASSRR57 pKa = 11.84RR58 pKa = 11.84CSGGLLRR65 pKa = 11.84LPPTVDD71 pKa = 2.94AFRR74 pKa = 11.84SEE76 pKa = 3.95PHH78 pKa = 5.38TLYY81 pKa = 10.18MSRR84 pKa = 11.84LMAMRR89 pKa = 11.84VKK91 pKa = 10.72ACGPSFLKK99 pKa = 10.15TDD101 pKa = 3.43SDD103 pKa = 4.02PPRR106 pKa = 11.84NGGSQRR112 pKa = 11.84VFTLPEE118 pKa = 3.97ILNSSS123 pKa = 3.6

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4504

0

4504

1561466

29

8590

346.7

38.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.393 ± 0.04

1.099 ± 0.016

6.332 ± 0.038

5.689 ± 0.04

4.087 ± 0.024

6.915 ± 0.037

2.369 ± 0.023

6.044 ± 0.03

4.364 ± 0.038

10.012 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.029

4.127 ± 0.03

4.258 ± 0.027

4.418 ± 0.034

4.923 ± 0.031

6.66 ± 0.039

5.661 ± 0.043

6.923 ± 0.034

1.282 ± 0.016

2.996 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski