Amino acid dipepetide frequency for BD1-7 clade bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.157AlaAla: 9.157 ± 0.119	1.196AlaCys: 1.196 ± 0.039	6.18AlaAsp: 6.18 ± 0.09	5.802AlaGlu: 5.802 ± 0.072	3.867AlaPhe: 3.867 ± 0.052	6.886AlaGly: 6.886 ± 0.092	1.905AlaHis: 1.905 ± 0.035	5.915AlaIle: 5.915 ± 0.069	3.953AlaLys: 3.953 ± 0.063	10.038AlaLeu: 10.038 ± 0.103	2.613AlaMet: 2.613 ± 0.046	3.852AlaAsn: 3.852 ± 0.061	3.432AlaPro: 3.432 ± 0.061	3.693AlaGln: 3.693 ± 0.057	4.198AlaArg: 4.198 ± 0.059	6.183AlaSer: 6.183 ± 0.084	4.934AlaThr: 4.934 ± 0.09	6.444AlaVal: 6.444 ± 0.073	1.111AlaTrp: 1.111 ± 0.028	2.552AlaTyr: 2.552 ± 0.042	0.019AlaXaa: 0.019 ± 0.003
Cys	0.941CysAla: 0.941 ± 0.026	0.154CysCys: 0.154 ± 0.009	0.729CysAsp: 0.729 ± 0.027	0.614CysGlu: 0.614 ± 0.024	0.503CysPhe: 0.503 ± 0.018	0.886CysGly: 0.886 ± 0.028	0.348CysHis: 0.348 ± 0.02	0.636CysIle: 0.636 ± 0.021	0.387CysLys: 0.387 ± 0.02	1.123CysLeu: 1.123 ± 0.033	0.249CysMet: 0.249 ± 0.013	0.392CysAsn: 0.392 ± 0.019	0.488CysPro: 0.488 ± 0.02	0.464CysGln: 0.464 ± 0.022	0.597CysArg: 0.597 ± 0.021	0.731CysSer: 0.731 ± 0.025	0.467CysThr: 0.467 ± 0.02	0.783CysVal: 0.783 ± 0.028	0.165CysTrp: 0.165 ± 0.01	0.328CysTyr: 0.328 ± 0.016	0.004CysXaa: 0.004 ± 0.002
Asp	6.165AspAla: 6.165 ± 0.083	0.63AspCys: 0.63 ± 0.023	4.327AspAsp: 4.327 ± 0.06	3.825AspGlu: 3.825 ± 0.057	2.553AspPhe: 2.553 ± 0.039	4.63AspGly: 4.63 ± 0.081	1.397AspHis: 1.397 ± 0.031	4.348AspIle: 4.348 ± 0.056	2.833AspLys: 2.833 ± 0.053	5.622AspLeu: 5.622 ± 0.069	1.418AspMet: 1.418 ± 0.038	2.795AspAsn: 2.795 ± 0.059	2.405AspPro: 2.405 ± 0.043	2.534AspGln: 2.534 ± 0.042	2.816AspArg: 2.816 ± 0.051	3.792AspSer: 3.792 ± 0.061	3.585AspThr: 3.585 ± 0.065	4.607AspVal: 4.607 ± 0.061	0.909AspTrp: 0.909 ± 0.023	2.124AspTyr: 2.124 ± 0.035	0.007AspXaa: 0.007 ± 0.002
Glu	5.289GluAla: 5.289 ± 0.066	0.526GluCys: 0.526 ± 0.022	2.949GluAsp: 2.949 ± 0.05	3.093GluGlu: 3.093 ± 0.06	2.256GluPhe: 2.256 ± 0.038	3.453GluGly: 3.453 ± 0.052	1.477GluHis: 1.477 ± 0.032	3.343GluIle: 3.343 ± 0.048	3.171GluLys: 3.171 ± 0.054	5.794GluLeu: 5.794 ± 0.068	1.318GluMet: 1.318 ± 0.03	2.544GluAsn: 2.544 ± 0.041	2.167GluPro: 2.167 ± 0.041	3.115GluGln: 3.115 ± 0.052	3.241GluArg: 3.241 ± 0.054	3.738GluSer: 3.738 ± 0.055	3.447GluThr: 3.447 ± 0.051	3.482GluVal: 3.482 ± 0.058	0.719GluTrp: 0.719 ± 0.021	1.764GluTyr: 1.764 ± 0.041	0.008GluXaa: 0.008 ± 0.002
Phe	3.623PheAla: 3.623 ± 0.051	0.561PheCys: 0.561 ± 0.022	3.221PheAsp: 3.221 ± 0.049	2.408PheGlu: 2.408 ± 0.037	1.776PhePhe: 1.776 ± 0.039	3.128PheGly: 3.128 ± 0.053	0.916PheHis: 0.916 ± 0.025	2.428PheIle: 2.428 ± 0.047	1.613PheLys: 1.613 ± 0.037	3.396PheLeu: 3.396 ± 0.069	0.976PheMet: 0.976 ± 0.027	1.851PheAsn: 1.851 ± 0.04	1.436PhePro: 1.436 ± 0.034	1.28PheGln: 1.28 ± 0.026	1.815PheArg: 1.815 ± 0.032	3.294PheSer: 3.294 ± 0.042	2.329PheThr: 2.329 ± 0.04	2.842PheVal: 2.842 ± 0.045	0.557PheTrp: 0.557 ± 0.021	1.415PheTyr: 1.415 ± 0.031	0.006PheXaa: 0.006 ± 0.002
Gly	5.666GlyAla: 5.666 ± 0.071	0.912GlyCys: 0.912 ± 0.027	4.415GlyAsp: 4.415 ± 0.084	3.93GlyGlu: 3.93 ± 0.062	3.542GlyPhe: 3.542 ± 0.051	4.975GlyGly: 4.975 ± 0.084	1.606GlyHis: 1.606 ± 0.034	4.616GlyIle: 4.616 ± 0.057	3.475GlyLys: 3.475 ± 0.053	6.815GlyLeu: 6.815 ± 0.075	1.895GlyMet: 1.895 ± 0.04	2.82GlyAsn: 2.82 ± 0.056	1.88GlyPro: 1.88 ± 0.036	2.71GlyGln: 2.71 ± 0.047	3.212GlyArg: 3.212 ± 0.055	4.436GlySer: 4.436 ± 0.07	3.544GlyThr: 3.544 ± 0.057	5.139GlyVal: 5.139 ± 0.067	1.034GlyTrp: 1.034 ± 0.026	2.511GlyTyr: 2.511 ± 0.042	0.013GlyXaa: 0.013 ± 0.003
His	2.003HisAla: 2.003 ± 0.039	0.362HisCys: 0.362 ± 0.016	1.268HisAsp: 1.268 ± 0.032	1.146HisGlu: 1.146 ± 0.028	1.1HisPhe: 1.1 ± 0.031	1.602HisGly: 1.602 ± 0.031	0.788HisHis: 0.788 ± 0.025	1.403HisIle: 1.403 ± 0.031	0.977HisLys: 0.977 ± 0.026	2.4HisLeu: 2.4 ± 0.043	0.563HisMet: 0.563 ± 0.022	0.849HisAsn: 0.849 ± 0.025	1.223HisPro: 1.223 ± 0.029	1.264HisGln: 1.264 ± 0.035	1.281HisArg: 1.281 ± 0.029	1.543HisSer: 1.543 ± 0.03	1.248HisThr: 1.248 ± 0.028	1.396HisVal: 1.396 ± 0.031	0.43HisTrp: 0.43 ± 0.017	0.842HisTyr: 0.842 ± 0.024	0.003HisXaa: 0.003 ± 0.001
Ile	6.297IleAla: 6.297 ± 0.074	0.7IleCys: 0.7 ± 0.02	4.756IleAsp: 4.756 ± 0.05	4.266IleGlu: 4.266 ± 0.055	2.047IlePhe: 2.047 ± 0.044	4.45IleGly: 4.45 ± 0.06	1.338IleHis: 1.338 ± 0.031	3.038IleIle: 3.038 ± 0.054	2.612IleLys: 2.612 ± 0.05	4.634IleLeu: 4.634 ± 0.071	1.186IleMet: 1.186 ± 0.034	2.83IleAsn: 2.83 ± 0.046	2.672IlePro: 2.672 ± 0.04	2.277IleGln: 2.277 ± 0.041	3.053IleArg: 3.053 ± 0.044	4.245IleSer: 4.245 ± 0.047	3.549IleThr: 3.549 ± 0.061	4.115IleVal: 4.115 ± 0.058	0.646IleTrp: 0.646 ± 0.022	1.718IleTyr: 1.718 ± 0.034	0.008IleXaa: 0.008 ± 0.002
Lys	4.505LysAla: 4.505 ± 0.07	0.305LysCys: 0.305 ± 0.015	2.429LysAsp: 2.429 ± 0.051	2.178LysGlu: 2.178 ± 0.049	1.222LysPhe: 1.222 ± 0.032	2.762LysGly: 2.762 ± 0.048	1.186LysHis: 1.186 ± 0.03	2.378LysIle: 2.378 ± 0.044	2.324LysLys: 2.324 ± 0.055	4.092LysLeu: 4.092 ± 0.059	0.982LysMet: 0.982 ± 0.029	1.982LysAsn: 1.982 ± 0.041	2.458LysPro: 2.458 ± 0.046	2.14LysGln: 2.14 ± 0.044	2.535LysArg: 2.535 ± 0.048	2.851LysSer: 2.851 ± 0.047	2.901LysThr: 2.901 ± 0.05	2.85LysVal: 2.85 ± 0.049	0.406LysTrp: 0.406 ± 0.019	1.146LysTyr: 1.146 ± 0.031	0.004LysXaa: 0.004 ± 0.002
Leu	9.913LeuAla: 9.913 ± 0.093	1.062LeuCys: 1.062 ± 0.031	6.192LeuAsp: 6.192 ± 0.065	5.48LeuGlu: 5.48 ± 0.073	3.845LeuPhe: 3.845 ± 0.065	6.644LeuGly: 6.644 ± 0.083	2.155LeuHis: 2.155 ± 0.043	5.978LeuIle: 5.978 ± 0.075	4.715LeuLys: 4.715 ± 0.069	9.923LeuLeu: 9.923 ± 0.125	2.607LeuMet: 2.607 ± 0.05	4.257LeuAsn: 4.257 ± 0.054	5.025LeuPro: 5.025 ± 0.067	4.104LeuGln: 4.104 ± 0.067	4.537LeuArg: 4.537 ± 0.062	7.354LeuSer: 7.354 ± 0.081	5.884LeuThr: 5.884 ± 0.08	6.786LeuVal: 6.786 ± 0.084	1.093LeuTrp: 1.093 ± 0.027	2.55LeuTyr: 2.55 ± 0.045	0.013LeuXaa: 0.013 ± 0.003
Met	2.493MetAla: 2.493 ± 0.048	0.207MetCys: 0.207 ± 0.013	1.289MetAsp: 1.289 ± 0.029	1.105MetGlu: 1.105 ± 0.03	0.818MetPhe: 0.818 ± 0.025	1.574MetGly: 1.574 ± 0.035	0.592MetHis: 0.592 ± 0.022	1.424MetIle: 1.424 ± 0.036	1.191MetLys: 1.191 ± 0.028	2.637MetLeu: 2.637 ± 0.053	0.72MetMet: 0.72 ± 0.024	0.983MetAsn: 0.983 ± 0.024	1.375MetPro: 1.375 ± 0.034	1.226MetGln: 1.226 ± 0.031	1.269MetArg: 1.269 ± 0.03	1.655MetSer: 1.655 ± 0.039	1.519MetThr: 1.519 ± 0.033	1.57MetVal: 1.57 ± 0.034	0.172MetTrp: 0.172 ± 0.013	0.494MetTyr: 0.494 ± 0.019	0.004MetXaa: 0.004 ± 0.002
Asn	3.966AsnAla: 3.966 ± 0.063	0.417AsnCys: 0.417 ± 0.019	2.589AsnAsp: 2.589 ± 0.046	2.055AsnGlu: 2.055 ± 0.042	1.445AsnPhe: 1.445 ± 0.034	3.168AsnGly: 3.168 ± 0.054	1.026AsnHis: 1.026 ± 0.027	2.557AsnIle: 2.557 ± 0.047	1.77AsnLys: 1.77 ± 0.036	3.668AsnLeu: 3.668 ± 0.052	0.834AsnMet: 0.834 ± 0.027	1.988AsnAsn: 1.988 ± 0.048	2.237AsnPro: 2.237 ± 0.045	1.899AsnGln: 1.899 ± 0.036	2.158AsnArg: 2.158 ± 0.04	2.383AsnSer: 2.383 ± 0.049	2.746AsnThr: 2.746 ± 0.062	2.603AsnVal: 2.603 ± 0.049	0.53AsnTrp: 0.53 ± 0.022	1.23AsnTyr: 1.23 ± 0.031	0.007AsnXaa: 0.007 ± 0.002
Pro	4.208ProAla: 4.208 ± 0.054	0.359ProCys: 0.359 ± 0.017	3.02ProAsp: 3.02 ± 0.048	3.183ProGlu: 3.183 ± 0.053	1.743ProPhe: 1.743 ± 0.034	2.854ProGly: 2.854 ± 0.046	0.902ProHis: 0.902 ± 0.024	2.474ProIle: 2.474 ± 0.038	1.803ProLys: 1.803 ± 0.038	4.221ProLeu: 4.221 ± 0.064	1.074ProMet: 1.074 ± 0.03	1.643ProAsn: 1.643 ± 0.034	1.471ProPro: 1.471 ± 0.037	1.688ProGln: 1.688 ± 0.036	1.559ProArg: 1.559 ± 0.038	2.741ProSer: 2.741 ± 0.043	2.412ProThr: 2.412 ± 0.047	3.449ProVal: 3.449 ± 0.053	0.57ProTrp: 0.57 ± 0.021	1.201ProTyr: 1.201 ± 0.032	0.005ProXaa: 0.005 ± 0.002
Gln	4.429GlnAla: 4.429 ± 0.069	0.456GlnCys: 0.456 ± 0.019	1.918GlnAsp: 1.918 ± 0.037	1.898GlnGlu: 1.898 ± 0.04	1.622GlnPhe: 1.622 ± 0.031	2.615GlnGly: 2.615 ± 0.043	1.233GlnHis: 1.233 ± 0.03	2.459GlnIle: 2.459 ± 0.037	1.916GlnLys: 1.916 ± 0.038	5.023GlnLeu: 5.023 ± 0.08	1.037GlnMet: 1.037 ± 0.027	1.527GlnAsn: 1.527 ± 0.031	1.973GlnPro: 1.973 ± 0.046	3.028GlnGln: 3.028 ± 0.078	2.669GlnArg: 2.669 ± 0.047	2.82GlnSer: 2.82 ± 0.05	2.582GlnThr: 2.582 ± 0.048	2.895GlnVal: 2.895 ± 0.043	0.791GlnTrp: 0.791 ± 0.027	1.285GlnTyr: 1.285 ± 0.032	0.007GlnXaa: 0.007 ± 0.002
Arg	3.837ArgAla: 3.837 ± 0.064	0.547ArgCys: 0.547 ± 0.019	2.755ArgAsp: 2.755 ± 0.042	2.875ArgGlu: 2.875 ± 0.048	2.533ArgPhe: 2.533 ± 0.041	2.708ArgGly: 2.708 ± 0.046	1.33ArgHis: 1.33 ± 0.032	3.241ArgIle: 3.241 ± 0.052	2.198ArgLys: 2.198 ± 0.048	5.652ArgLeu: 5.652 ± 0.073	1.317ArgMet: 1.317 ± 0.034	1.798ArgAsn: 1.798 ± 0.033	1.798ArgPro: 1.798 ± 0.033	2.674ArgGln: 2.674 ± 0.047	2.64ArgArg: 2.64 ± 0.055	2.94ArgSer: 2.94 ± 0.045	2.245ArgThr: 2.245 ± 0.042	3.422ArgVal: 3.422 ± 0.047	0.783ArgTrp: 0.783 ± 0.025	1.926ArgTyr: 1.926 ± 0.038	0.008ArgXaa: 0.008 ± 0.002
Ser	6.388SerAla: 6.388 ± 0.083	0.647SerCys: 0.647 ± 0.025	4.457SerAsp: 4.457 ± 0.087	3.762SerGlu: 3.762 ± 0.06	2.658SerPhe: 2.658 ± 0.044	5.204SerGly: 5.204 ± 0.078	1.532SerHis: 1.532 ± 0.034	4.024SerIle: 4.024 ± 0.051	2.536SerLys: 2.536 ± 0.044	6.831SerLeu: 6.831 ± 0.075	1.677SerMet: 1.677 ± 0.031	2.567SerAsn: 2.567 ± 0.053	2.772SerPro: 2.772 ± 0.04	2.676SerGln: 2.676 ± 0.043	3.21SerArg: 3.21 ± 0.045	4.466SerSer: 4.466 ± 0.082	3.647SerThr: 3.647 ± 0.063	4.804SerVal: 4.804 ± 0.066	0.827SerTrp: 0.827 ± 0.025	1.912SerTyr: 1.912 ± 0.038	0.006SerXaa: 0.006 ± 0.002
Thr	5.27ThrAla: 5.27 ± 0.092	0.569ThrCys: 0.569 ± 0.023	3.657ThrAsp: 3.657 ± 0.062	2.991ThrGlu: 2.991 ± 0.046	2.237ThrPhe: 2.237 ± 0.04	4.273ThrGly: 4.273 ± 0.068	1.405ThrHis: 1.405 ± 0.035	3.381ThrIle: 3.381 ± 0.052	1.737ThrLys: 1.737 ± 0.044	6.533ThrLeu: 6.533 ± 0.073	1.13ThrMet: 1.13 ± 0.031	2.119ThrAsn: 2.119 ± 0.049	3.139ThrPro: 3.139 ± 0.058	2.393ThrGln: 2.393 ± 0.048	2.617ThrArg: 2.617 ± 0.041	3.575ThrSer: 3.575 ± 0.063	3.31ThrThr: 3.31 ± 0.06	3.967ThrVal: 3.967 ± 0.073	0.665ThrTrp: 0.665 ± 0.022	1.638ThrTyr: 1.638 ± 0.039	0.008ThrXaa: 0.008 ± 0.002
Val	6.395ValAla: 6.395 ± 0.073	0.826ValCys: 0.826 ± 0.024	4.772ValAsp: 4.772 ± 0.073	4.186ValGlu: 4.186 ± 0.057	3.088ValPhe: 3.088 ± 0.05	4.566ValGly: 4.566 ± 0.075	1.34ValHis: 1.34 ± 0.03	4.551ValIle: 4.551 ± 0.061	2.889ValLys: 2.889 ± 0.047	6.673ValLeu: 6.673 ± 0.072	1.746ValMet: 1.746 ± 0.045	2.945ValAsn: 2.945 ± 0.051	2.695ValPro: 2.695 ± 0.046	2.254ValGln: 2.254 ± 0.043	3.026ValArg: 3.026 ± 0.05	5.168ValSer: 5.168 ± 0.068	4.075ValThr: 4.075 ± 0.072	5.295ValVal: 5.295 ± 0.077	0.777ValTrp: 0.777 ± 0.023	1.957ValTyr: 1.957 ± 0.041	0.011ValXaa: 0.011 ± 0.002
Trp	0.919TrpAla: 0.919 ± 0.025	0.147TrpCys: 0.147 ± 0.011	0.628TrpAsp: 0.628 ± 0.021	0.55TrpGlu: 0.55 ± 0.021	0.591TrpPhe: 0.591 ± 0.02	0.756TrpGly: 0.756 ± 0.026	0.412TrpHis: 0.412 ± 0.017	0.747TrpIle: 0.747 ± 0.025	0.46TrpLys: 0.46 ± 0.019	1.716TrpLeu: 1.716 ± 0.038	0.351TrpMet: 0.351 ± 0.016	0.457TrpAsn: 0.457 ± 0.019	0.525TrpPro: 0.525 ± 0.019	1.007TrpGln: 1.007 ± 0.031	0.781TrpArg: 0.781 ± 0.025	0.728TrpSer: 0.728 ± 0.026	0.559TrpThr: 0.559 ± 0.022	0.913TrpVal: 0.913 ± 0.023	0.167TrpTrp: 0.167 ± 0.01	0.401TrpTyr: 0.401 ± 0.018	0.003TrpXaa: 0.003 ± 0.002
Tyr	2.453TyrAla: 2.453 ± 0.044	0.407TyrCys: 0.407 ± 0.019	1.763TyrAsp: 1.763 ± 0.041	1.54TyrGlu: 1.54 ± 0.032	1.416TyrPhe: 1.416 ± 0.03	2.003TyrGly: 2.003 ± 0.038	0.801TyrHis: 0.801 ± 0.023	1.481TyrIle: 1.481 ± 0.034	1.068TyrLys: 1.068 ± 0.026	3.31TyrLeu: 3.31 ± 0.048	0.619TyrMet: 0.619 ± 0.022	1.07TyrAsn: 1.07 ± 0.027	1.396TyrPro: 1.396 ± 0.033	1.752TyrGln: 1.752 ± 0.036	2.005TyrArg: 2.005 ± 0.041	1.957TyrSer: 1.957 ± 0.042	1.626TyrThr: 1.626 ± 0.035	1.862TyrVal: 1.862 ± 0.034	0.466TyrTrp: 0.466 ± 0.02	0.967TyrTyr: 0.967 ± 0.025	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.013XaaAla: 0.013 ± 0.003	0.001XaaCys: 0.001 ± 0.001	0.009XaaAsp: 0.009 ± 0.003	0.005XaaGlu: 0.005 ± 0.002	0.006XaaPhe: 0.006 ± 0.002	0.01XaaGly: 0.01 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.015XaaIle: 0.015 ± 0.003	0.009XaaLys: 0.009 ± 0.002	0.017XaaLeu: 0.017 ± 0.003	0.004XaaMet: 0.004 ± 0.002	0.006XaaAsn: 0.006 ± 0.002	0.014XaaPro: 0.014 ± 0.003	0.004XaaGln: 0.004 ± 0.002	0.009XaaArg: 0.009 ± 0.002	0.005XaaSer: 0.005 ± 0.002	0.004XaaThr: 0.004 ± 0.002	0.01XaaVal: 0.01 ± 0.003	0.001XaaTrp: 0.001 ± 0.001	0.005XaaTyr: 0.005 ± 0.002	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4504 proteins (1561466 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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