Sutterella wadsworthensis HGA0223
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2393 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S3BG13|S3BG13_9BURK ABC transporter domain-containing protein OS=Sutterella wadsworthensis HGA0223 OX=1203554 GN=HMPREF1476_01080 PE=4 SV=1
MM1 pKa = 8.25 SEE3 pKa = 4.22 EE4 pKa = 4.05 DD5 pKa = 2.85 TGAPHH10 pKa = 7.08 FATGAEE16 pKa = 4.28 VEE18 pKa = 4.28 ALLDD22 pKa = 3.27 KK23 pKa = 10.67 YY24 pKa = 11.44 ARR26 pKa = 11.84 DD27 pKa = 3.21 RR28 pKa = 11.84 VGAHH32 pKa = 5.57 TRR34 pKa = 11.84 MQCKK38 pKa = 9.36 VCWYY42 pKa = 10.11 VYY44 pKa = 10.76 NPDD47 pKa = 3.52 EE48 pKa = 4.72 GCPEE52 pKa = 3.92 EE53 pKa = 6.58 AIDD56 pKa = 4.83 PGTPFLDD63 pKa = 4.0 LPDD66 pKa = 5.6 DD67 pKa = 4.88 FVCPDD72 pKa = 3.64 CGHH75 pKa = 7.47 PKK77 pKa = 9.17 TAFLPLDD84 pKa = 4.82 DD85 pKa = 5.39 DD86 pKa = 5.1 QIDD89 pKa = 3.82 PSDD92 pKa = 3.93 PSGPAQGWSDD102 pKa = 3.21 RR103 pKa = 11.84 WEE105 pKa = 4.19 HH106 pKa = 5.67 GG107 pKa = 3.28
Molecular weight: 11.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.809
IPC2_protein 4.037
IPC_protein 4.012
Toseland 3.808
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.846
Grimsley 3.719
Solomon 3.999
Lehninger 3.948
Nozaki 4.126
DTASelect 4.368
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.139
Patrickios 1.952
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.024
Protein with the highest isoelectric point:
>tr|S3BEN8|S3BEN8_9BURK Translation initiation factor IF-1 OS=Sutterella wadsworthensis HGA0223 OX=1203554 GN=infA PE=3 SV=1
MM1 pKa = 7.15 VRR3 pKa = 11.84 TEE5 pKa = 3.8 ALKK8 pKa = 10.72 YY9 pKa = 9.93 IQLLHH14 pKa = 6.11 EE15 pKa = 4.93 RR16 pKa = 11.84 GEE18 pKa = 4.46 GSLKK22 pKa = 9.67 ISKK25 pKa = 10.37 RR26 pKa = 11.84 LGLSRR31 pKa = 11.84 NTVRR35 pKa = 11.84 KK36 pKa = 9.61 YY37 pKa = 10.66 LALLDD42 pKa = 3.93 SGQPIVLRR50 pKa = 11.84 VQRR53 pKa = 11.84 ARR55 pKa = 11.84 ALDD58 pKa = 4.01 PYY60 pKa = 10.87 EE61 pKa = 4.16 EE62 pKa = 4.34 VIKK65 pKa = 10.77 RR66 pKa = 11.84 LFVDD70 pKa = 4.9 CRR72 pKa = 11.84 GHH74 pKa = 6.67 CPNLQRR80 pKa = 11.84 RR81 pKa = 11.84 LKK83 pKa = 9.07 EE84 pKa = 3.87 QLGLAVQLRR93 pKa = 11.84 TLQKK97 pKa = 10.39 FCQPWRR103 pKa = 11.84 KK104 pKa = 7.31 TVLTPQEE111 pKa = 3.62 RR112 pKa = 11.84 VVRR115 pKa = 11.84 YY116 pKa = 8.05 EE117 pKa = 3.65 VNLPDD122 pKa = 3.5 RR123 pKa = 4.76
Molecular weight: 14.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.619
IPC_protein 10.335
Toseland 10.613
ProMoST 10.452
Dawson 10.716
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.95
Grimsley 10.76
Solomon 10.818
Lehninger 10.774
Nozaki 10.599
DTASelect 10.409
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.672
IPC_peptide 10.818
IPC2_peptide 9.472
IPC2.peptide.svr19 8.635
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2393
0
2393
840102
21
2064
351.1
38.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.704 ± 0.066
1.177 ± 0.019
5.231 ± 0.036
6.106 ± 0.046
4.075 ± 0.03
7.771 ± 0.049
1.852 ± 0.022
5.331 ± 0.036
4.581 ± 0.043
10.051 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.539 ± 0.026
3.084 ± 0.033
4.755 ± 0.036
3.155 ± 0.028
6.117 ± 0.049
5.984 ± 0.04
5.496 ± 0.049
7.25 ± 0.039
1.341 ± 0.021
2.401 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here