Amino acid dipepetide frequency for Flavihumibacter sp. ZG627

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.19AlaAla: 7.19 ± 0.108
0.76AlaCys: 0.76 ± 0.031
4.238AlaAsp: 4.238 ± 0.061
4.525AlaGlu: 4.525 ± 0.064
3.626AlaPhe: 3.626 ± 0.055
6.221AlaGly: 6.221 ± 0.095
1.245AlaHis: 1.245 ± 0.038
5.796AlaIle: 5.796 ± 0.078
4.136AlaLys: 4.136 ± 0.072
7.155AlaLeu: 7.155 ± 0.092
2.03AlaMet: 2.03 ± 0.049
3.426AlaAsn: 3.426 ± 0.061
2.564AlaPro: 2.564 ± 0.059
2.538AlaGln: 2.538 ± 0.05
3.271AlaArg: 3.271 ± 0.056
4.888AlaSer: 4.888 ± 0.076
4.132AlaThr: 4.132 ± 0.067
5.058AlaVal: 5.058 ± 0.078
0.883AlaTrp: 0.883 ± 0.03
2.589AlaTyr: 2.589 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.523CysAla: 0.523 ± 0.027
0.149CysCys: 0.149 ± 0.011
0.39CysAsp: 0.39 ± 0.019
0.432CysGlu: 0.432 ± 0.026
0.416CysPhe: 0.416 ± 0.021
0.7CysGly: 0.7 ± 0.032
0.194CysHis: 0.194 ± 0.015
0.668CysIle: 0.668 ± 0.027
0.402CysLys: 0.402 ± 0.022
0.738CysLeu: 0.738 ± 0.029
0.225CysMet: 0.225 ± 0.015
0.417CysAsn: 0.417 ± 0.02
0.386CysPro: 0.386 ± 0.023
0.236CysGln: 0.236 ± 0.014
0.369CysArg: 0.369 ± 0.018
0.619CysSer: 0.619 ± 0.026
0.478CysThr: 0.478 ± 0.024
0.44CysVal: 0.44 ± 0.017
0.107CysTrp: 0.107 ± 0.009
0.313CysTyr: 0.313 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
4.195AspAla: 4.195 ± 0.064
0.426AspCys: 0.426 ± 0.024
2.552AspAsp: 2.552 ± 0.055
3.221AspGlu: 3.221 ± 0.062
2.745AspPhe: 2.745 ± 0.055
4.005AspGly: 4.005 ± 0.081
1.069AspHis: 1.069 ± 0.029
4.02AspIle: 4.02 ± 0.063
3.523AspLys: 3.523 ± 0.061
4.915AspLeu: 4.915 ± 0.073
1.299AspMet: 1.299 ± 0.033
2.546AspAsn: 2.546 ± 0.056
2.449AspPro: 2.449 ± 0.047
1.687AspGln: 1.687 ± 0.043
2.313AspArg: 2.313 ± 0.043
3.164AspSer: 3.164 ± 0.066
2.493AspThr: 2.493 ± 0.049
3.016AspVal: 3.016 ± 0.063
0.779AspTrp: 0.779 ± 0.024
2.209AspTyr: 2.209 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
4.629GluAla: 4.629 ± 0.076
0.364GluCys: 0.364 ± 0.02
2.718GluAsp: 2.718 ± 0.052
4.283GluGlu: 4.283 ± 0.091
2.464GluPhe: 2.464 ± 0.048
3.929GluGly: 3.929 ± 0.065
1.133GluHis: 1.133 ± 0.032
4.207GluIle: 4.207 ± 0.063
5.134GluLys: 5.134 ± 0.085
6.024GluLeu: 6.024 ± 0.088
1.906GluMet: 1.906 ± 0.046
3.15GluAsn: 3.15 ± 0.056
1.861GluPro: 1.861 ± 0.044
2.455GluGln: 2.455 ± 0.048
2.689GluArg: 2.689 ± 0.057
3.132GluSer: 3.132 ± 0.053
2.978GluThr: 2.978 ± 0.054
3.775GluVal: 3.775 ± 0.071
0.872GluTrp: 0.872 ± 0.027
2.08GluTyr: 2.08 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.259PheAla: 3.259 ± 0.057
0.452PheCys: 0.452 ± 0.021
2.802PheAsp: 2.802 ± 0.056
2.699PheGlu: 2.699 ± 0.045
2.425PhePhe: 2.425 ± 0.058
3.515PheGly: 3.515 ± 0.066
0.936PheHis: 0.936 ± 0.029
3.414PheIle: 3.414 ± 0.063
2.376PheLys: 2.376 ± 0.044
4.542PheLeu: 4.542 ± 0.07
1.128PheMet: 1.128 ± 0.03
2.668PheAsn: 2.668 ± 0.051
1.844PhePro: 1.844 ± 0.043
1.519PheGln: 1.519 ± 0.039
2.532PheArg: 2.532 ± 0.047
3.675PheSer: 3.675 ± 0.066
3.016PheThr: 3.016 ± 0.06
2.602PheVal: 2.602 ± 0.048
0.583PheTrp: 0.583 ± 0.025
1.925PheTyr: 1.925 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
5.033GlyAla: 5.033 ± 0.07
0.691GlyCys: 0.691 ± 0.034
3.666GlyAsp: 3.666 ± 0.065
3.811GlyGlu: 3.811 ± 0.062
3.789GlyPhe: 3.789 ± 0.066
5.549GlyGly: 5.549 ± 0.101
1.294GlyHis: 1.294 ± 0.036
5.85GlyIle: 5.85 ± 0.07
5.359GlyLys: 5.359 ± 0.076
6.243GlyLeu: 6.243 ± 0.083
2.069GlyMet: 2.069 ± 0.046
3.935GlyAsn: 3.935 ± 0.086
1.707GlyPro: 1.707 ± 0.041
2.151GlyGln: 2.151 ± 0.041
3.091GlyArg: 3.091 ± 0.054
4.785GlySer: 4.785 ± 0.074
4.113GlyThr: 4.113 ± 0.082
4.49GlyVal: 4.49 ± 0.069
1.158GlyTrp: 1.158 ± 0.04
2.932GlyTyr: 2.932 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.327HisAla: 1.327 ± 0.037
0.2HisCys: 0.2 ± 0.013
0.94HisAsp: 0.94 ± 0.036
1.038HisGlu: 1.038 ± 0.031
1.178HisPhe: 1.178 ± 0.033
1.271HisGly: 1.271 ± 0.035
0.617HisHis: 0.617 ± 0.028
1.393HisIle: 1.393 ± 0.036
1.009HisLys: 1.009 ± 0.029
1.975HisLeu: 1.975 ± 0.049
0.447HisMet: 0.447 ± 0.022
0.922HisAsn: 0.922 ± 0.026
1.182HisPro: 1.182 ± 0.032
0.799HisGln: 0.799 ± 0.03
0.917HisArg: 0.917 ± 0.031
1.17HisSer: 1.17 ± 0.034
1.014HisThr: 1.014 ± 0.03
0.982HisVal: 0.982 ± 0.033
0.305HisTrp: 0.305 ± 0.02
0.908HisTyr: 0.908 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.817IleAla: 5.817 ± 0.078
0.679IleCys: 0.679 ± 0.024
4.15IleAsp: 4.15 ± 0.072
4.169IleGlu: 4.169 ± 0.065
3.145IlePhe: 3.145 ± 0.06
5.003IleGly: 5.003 ± 0.077
1.453IleHis: 1.453 ± 0.038
5.298IleIle: 5.298 ± 0.087
3.976IleLys: 3.976 ± 0.073
6.53IleLeu: 6.53 ± 0.094
1.573IleMet: 1.573 ± 0.042
3.986IleAsn: 3.986 ± 0.066
3.443IlePro: 3.443 ± 0.053
2.243IleGln: 2.243 ± 0.047
3.948IleArg: 3.948 ± 0.057
5.351IleSer: 5.351 ± 0.072
4.379IleThr: 4.379 ± 0.077
4.175IleVal: 4.175 ± 0.069
0.73IleTrp: 0.73 ± 0.024
2.465IleTyr: 2.465 ± 0.052
0.0IleXaa: 0.0 ± 0.0
Lys
4.858LysAla: 4.858 ± 0.079
0.337LysCys: 0.337 ± 0.02
3.58LysAsp: 3.58 ± 0.066
4.748LysGlu: 4.748 ± 0.086
2.273LysPhe: 2.273 ± 0.04
4.499LysGly: 4.499 ± 0.07
1.191LysHis: 1.191 ± 0.037
4.233LysIle: 4.233 ± 0.064
4.971LysLys: 4.971 ± 0.081
5.828LysLeu: 5.828 ± 0.074
1.929LysMet: 1.929 ± 0.045
3.201LysAsn: 3.201 ± 0.064
2.573LysPro: 2.573 ± 0.05
2.591LysGln: 2.591 ± 0.055
2.674LysArg: 2.674 ± 0.061
3.481LysSer: 3.481 ± 0.061
3.351LysThr: 3.351 ± 0.052
3.958LysVal: 3.958 ± 0.076
0.848LysTrp: 0.848 ± 0.03
2.308LysTyr: 2.308 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
7.014LeuAla: 7.014 ± 0.086
0.741LeuCys: 0.741 ± 0.029
4.531LeuAsp: 4.531 ± 0.061
5.508LeuGlu: 5.508 ± 0.09
4.702LeuPhe: 4.702 ± 0.071
6.029LeuGly: 6.029 ± 0.083
1.986LeuHis: 1.986 ± 0.048
6.103LeuIle: 6.103 ± 0.089
6.203LeuLys: 6.203 ± 0.087
10.235LeuLeu: 10.235 ± 0.141
2.491LeuMet: 2.491 ± 0.059
4.848LeuAsn: 4.848 ± 0.073
4.422LeuPro: 4.422 ± 0.06
4.312LeuGln: 4.312 ± 0.077
4.396LeuArg: 4.396 ± 0.063
6.627LeuSer: 6.627 ± 0.082
4.968LeuThr: 4.968 ± 0.08
6.127LeuVal: 6.127 ± 0.073
0.981LeuTrp: 0.981 ± 0.032
3.324LeuTyr: 3.324 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
2.258MetAla: 2.258 ± 0.046
0.126MetCys: 0.126 ± 0.011
1.435MetAsp: 1.435 ± 0.037
1.742MetGlu: 1.742 ± 0.04
0.892MetPhe: 0.892 ± 0.03
1.847MetGly: 1.847 ± 0.036
0.543MetHis: 0.543 ± 0.024
1.751MetIle: 1.751 ± 0.042
2.037MetLys: 2.037 ± 0.049
2.426MetLeu: 2.426 ± 0.047
0.805MetMet: 0.805 ± 0.03
1.342MetAsn: 1.342 ± 0.039
1.229MetPro: 1.229 ± 0.033
1.225MetGln: 1.225 ± 0.032
1.224MetArg: 1.224 ± 0.034
1.433MetSer: 1.433 ± 0.037
1.2MetThr: 1.2 ± 0.035
1.878MetVal: 1.878 ± 0.044
0.22MetTrp: 0.22 ± 0.013
0.669MetTyr: 0.669 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.912AsnAla: 3.912 ± 0.071
0.437AsnCys: 0.437 ± 0.025
2.789AsnAsp: 2.789 ± 0.062
2.935AsnGlu: 2.935 ± 0.053
2.33AsnPhe: 2.33 ± 0.059
4.006AsnGly: 4.006 ± 0.081
0.948AsnHis: 0.948 ± 0.03
4.047AsnIle: 4.047 ± 0.069
3.146AsnLys: 3.146 ± 0.057
4.491AsnLeu: 4.491 ± 0.065
1.264AsnMet: 1.264 ± 0.034
2.976AsnAsn: 2.976 ± 0.076
2.59AsnPro: 2.59 ± 0.05
1.784AsnGln: 1.784 ± 0.041
2.449AsnArg: 2.449 ± 0.053
3.081AsnSer: 3.081 ± 0.072
2.979AsnThr: 2.979 ± 0.057
2.607AsnVal: 2.607 ± 0.053
0.794AsnTrp: 0.794 ± 0.028
2.264AsnTyr: 2.264 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
3.616ProAla: 3.616 ± 0.071
0.254ProCys: 0.254 ± 0.017
2.724ProAsp: 2.724 ± 0.045
2.905ProGlu: 2.905 ± 0.052
2.053ProPhe: 2.053 ± 0.042
3.158ProGly: 3.158 ± 0.051
0.784ProHis: 0.784 ± 0.034
2.397ProIle: 2.397 ± 0.046
1.968ProLys: 1.968 ± 0.045
3.61ProLeu: 3.61 ± 0.061
0.968ProMet: 0.968 ± 0.033
1.773ProAsn: 1.773 ± 0.041
1.282ProPro: 1.282 ± 0.038
1.338ProGln: 1.338 ± 0.032
1.401ProArg: 1.401 ± 0.037
2.514ProSer: 2.514 ± 0.052
2.014ProThr: 2.014 ± 0.048
3.453ProVal: 3.453 ± 0.06
0.476ProTrp: 0.476 ± 0.022
1.492ProTyr: 1.492 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.481GlnAla: 2.481 ± 0.051
0.226GlnCys: 0.226 ± 0.014
1.507GlnAsp: 1.507 ± 0.039
2.191GlnGlu: 2.191 ± 0.042
1.754GlnPhe: 1.754 ± 0.036
1.988GlnGly: 1.988 ± 0.05
0.83GlnHis: 0.83 ± 0.024
2.23GlnIle: 2.23 ± 0.041
2.427GlnLys: 2.427 ± 0.047
4.283GlnLeu: 4.283 ± 0.068
0.995GlnMet: 0.995 ± 0.03
1.619GlnAsn: 1.619 ± 0.045
1.634GlnPro: 1.634 ± 0.044
2.317GlnGln: 2.317 ± 0.057
1.702GlnArg: 1.702 ± 0.039
2.115GlnSer: 2.115 ± 0.045
1.822GlnThr: 1.822 ± 0.042
2.489GlnVal: 2.489 ± 0.057
0.521GlnTrp: 0.521 ± 0.022
1.545GlnTyr: 1.545 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.674ArgAla: 2.674 ± 0.051
0.269ArgCys: 0.269 ± 0.016
2.262ArgAsp: 2.262 ± 0.041
2.844ArgGlu: 2.844 ± 0.049
2.466ArgPhe: 2.466 ± 0.047
2.498ArgGly: 2.498 ± 0.051
0.911ArgHis: 0.911 ± 0.029
3.737ArgIle: 3.737 ± 0.06
3.434ArgLys: 3.434 ± 0.065
4.212ArgLeu: 4.212 ± 0.063
1.371ArgMet: 1.371 ± 0.038
2.856ArgAsn: 2.856 ± 0.053
1.593ArgPro: 1.593 ± 0.04
1.889ArgGln: 1.889 ± 0.042
2.064ArgArg: 2.064 ± 0.052
2.857ArgSer: 2.857 ± 0.05
2.301ArgThr: 2.301 ± 0.042
2.709ArgVal: 2.709 ± 0.047
0.619ArgTrp: 0.619 ± 0.026
2.021ArgTyr: 2.021 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
4.751SerAla: 4.751 ± 0.072
0.624SerCys: 0.624 ± 0.026
3.053SerAsp: 3.053 ± 0.052
3.18SerGlu: 3.18 ± 0.054
3.672SerPhe: 3.672 ± 0.063
5.198SerGly: 5.198 ± 0.078
1.131SerHis: 1.131 ± 0.034
4.972SerIle: 4.972 ± 0.073
3.483SerLys: 3.483 ± 0.056
6.51SerLeu: 6.51 ± 0.085
1.663SerMet: 1.663 ± 0.037
3.101SerAsn: 3.101 ± 0.06
2.487SerPro: 2.487 ± 0.052
1.988SerGln: 1.988 ± 0.047
2.924SerArg: 2.924 ± 0.051
4.454SerSer: 4.454 ± 0.088
3.45SerThr: 3.45 ± 0.054
4.06SerVal: 4.06 ± 0.065
0.919SerTrp: 0.919 ± 0.032
2.523SerTyr: 2.523 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.492ThrAla: 4.492 ± 0.072
0.385ThrCys: 0.385 ± 0.021
3.147ThrAsp: 3.147 ± 0.059
3.014ThrGlu: 3.014 ± 0.052
2.142ThrPhe: 2.142 ± 0.048
4.783ThrGly: 4.783 ± 0.077
1.017ThrHis: 1.017 ± 0.029
4.552ThrIle: 4.552 ± 0.072
2.623ThrLys: 2.623 ± 0.055
4.893ThrLeu: 4.893 ± 0.062
1.167ThrMet: 1.167 ± 0.035
2.695ThrAsn: 2.695 ± 0.063
2.471ThrPro: 2.471 ± 0.057
1.482ThrGln: 1.482 ± 0.035
2.389ThrArg: 2.389 ± 0.046
3.235ThrSer: 3.235 ± 0.065
3.258ThrThr: 3.258 ± 0.069
3.798ThrVal: 3.798 ± 0.074
0.695ThrTrp: 0.695 ± 0.026
1.885ThrTyr: 1.885 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
4.782ValAla: 4.782 ± 0.08
0.603ValCys: 0.603 ± 0.026
3.302ValAsp: 3.302 ± 0.067
3.783ValGlu: 3.783 ± 0.065
3.091ValPhe: 3.091 ± 0.059
3.953ValGly: 3.953 ± 0.062
1.149ValHis: 1.149 ± 0.036
4.625ValIle: 4.625 ± 0.075
4.012ValLys: 4.012 ± 0.073
5.963ValLeu: 5.963 ± 0.073
1.649ValMet: 1.649 ± 0.041
3.48ValAsn: 3.48 ± 0.064
2.509ValPro: 2.509 ± 0.048
2.051ValGln: 2.051 ± 0.043
2.623ValArg: 2.623 ± 0.052
4.25ValSer: 4.25 ± 0.071
3.452ValThr: 3.452 ± 0.065
4.411ValVal: 4.411 ± 0.071
0.705ValTrp: 0.705 ± 0.028
2.315ValTyr: 2.315 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.802TrpAla: 0.802 ± 0.029
0.105TrpCys: 0.105 ± 0.008
0.666TrpAsp: 0.666 ± 0.029
0.713TrpGlu: 0.713 ± 0.026
0.664TrpPhe: 0.664 ± 0.027
0.869TrpGly: 0.869 ± 0.033
0.305TrpHis: 0.305 ± 0.018
0.845TrpIle: 0.845 ± 0.032
0.964TrpLys: 0.964 ± 0.029
1.364TrpLeu: 1.364 ± 0.038
0.499TrpMet: 0.499 ± 0.021
0.758TrpAsn: 0.758 ± 0.03
0.38TrpPro: 0.38 ± 0.018
0.54TrpGln: 0.54 ± 0.023
0.611TrpArg: 0.611 ± 0.028
0.737TrpSer: 0.737 ± 0.027
0.641TrpThr: 0.641 ± 0.026
0.737TrpVal: 0.737 ± 0.028
0.243TrpTrp: 0.243 ± 0.015
0.512TrpTyr: 0.512 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.657TyrAla: 2.657 ± 0.05
0.375TyrCys: 0.375 ± 0.017
2.176TyrAsp: 2.176 ± 0.047
2.044TyrGlu: 2.044 ± 0.045
2.084TyrPhe: 2.084 ± 0.043
2.695TyrGly: 2.695 ± 0.049
0.858TyrHis: 0.858 ± 0.027
2.373TyrIle: 2.373 ± 0.042
2.251TyrLys: 2.251 ± 0.046
3.612TyrLeu: 3.612 ± 0.054
0.805TyrMet: 0.805 ± 0.028
2.129TyrAsn: 2.129 ± 0.053
1.573TyrPro: 1.573 ± 0.037
1.533TyrGln: 1.533 ± 0.035
2.001TyrArg: 2.001 ± 0.049
2.574TyrSer: 2.574 ± 0.051
2.083TyrThr: 2.083 ± 0.055
1.941TyrVal: 1.941 ± 0.042
0.517TyrTrp: 0.517 ± 0.022
1.642TyrTyr: 1.642 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3302 proteins (1139701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski