Klebsiella phage vB_KpnP_SU503
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5Q3V8|A0A0C5Q3V8_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_SU503 OX=1610834 GN=SU503_05 PE=4 SV=1
MM1 pKa = 7.26 VNVFNIIVTSAMLVLGNDD19 pKa = 3.45 VNNPVPYY26 pKa = 9.17 CTVQIQQQGQQPRR39 pKa = 11.84 PEE41 pKa = 3.91 YY42 pKa = 10.84 DD43 pKa = 3.05 LFEE46 pKa = 4.98 NPEE49 pKa = 4.37 GGCKK53 pKa = 9.74 EE54 pKa = 4.06 LGALVLEE61 pKa = 4.9 AVQEE65 pKa = 4.19 QEE67 pKa = 3.78 PNAAVTLTVDD77 pKa = 3.83 GVSNNDD83 pKa = 2.7 II84 pKa = 4.04
Molecular weight: 9.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.847
IPC2_protein 3.745
IPC_protein 3.554
Toseland 3.414
ProMoST 3.745
Dawson 3.554
Bjellqvist 3.732
Wikipedia 3.49
Rodwell 3.427
Grimsley 3.338
Solomon 3.49
Lehninger 3.452
Nozaki 3.706
DTASelect 3.795
Thurlkill 3.49
EMBOSS 3.503
Sillero 3.694
Patrickios 0.477
IPC_peptide 3.49
IPC2_peptide 3.656
IPC2.peptide.svr19 3.657
Protein with the highest isoelectric point:
>tr|A0A0C5PR04|A0A0C5PR04_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_SU503 OX=1610834 GN=SU503_04 PE=4 SV=1
MM1 pKa = 8.21 RR2 pKa = 11.84 IITWAKK8 pKa = 7.54 EE9 pKa = 3.66 QYY11 pKa = 11.04 AVFLLMRR18 pKa = 11.84 AQRR21 pKa = 11.84 LQQRR25 pKa = 11.84 ANDD28 pKa = 3.63 WHH30 pKa = 6.22 WAANSHH36 pKa = 5.18 AHH38 pKa = 6.13 RR39 pKa = 11.84 VSLLGNEE46 pKa = 4.31 ISAHH50 pKa = 6.35 RR51 pKa = 11.84 YY52 pKa = 8.79 HH53 pKa = 7.31 LTRR56 pKa = 11.84 QCAKK60 pKa = 9.58 SRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 AYY66 pKa = 10.62 ALGAEE71 pKa = 4.34 ATAAEE76 pKa = 4.57 TKK78 pKa = 10.24 AHH80 pKa = 5.86 NFISKK85 pKa = 10.22 HH86 pKa = 4.86 KK87 pKa = 10.71 LKK89 pKa = 11.07 GFDD92 pKa = 3.17
Molecular weight: 10.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.867
IPC_protein 10.76
Toseland 10.891
ProMoST 10.657
Dawson 10.979
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 11.184
Grimsley 11.023
Solomon 11.14
Lehninger 11.096
Nozaki 10.877
DTASelect 10.701
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.906
Patrickios 10.935
IPC_peptide 11.14
IPC2_peptide 9.721
IPC2.peptide.svr19 8.45
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
13588
51
1232
251.6
27.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.355 ± 0.599
1.303 ± 0.199
6.241 ± 0.216
5.895 ± 0.374
3.069 ± 0.166
7.426 ± 0.296
1.832 ± 0.154
4.578 ± 0.181
4.901 ± 0.306
8.846 ± 0.253
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.907 ± 0.213
4.092 ± 0.245
3.783 ± 0.246
4.806 ± 0.385
6.049 ± 0.328
6.219 ± 0.273
5.674 ± 0.299
6.903 ± 0.223
1.332 ± 0.099
3.79 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here