Nannocystis exedens
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9143 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2FED5|A0A1I2FED5_9DELT NAD-dependent protein deacetylase SIR2 family OS=Nannocystis exedens OX=54 GN=SAMN02745121_06555 PE=4 SV=1
MM1 pKa = 8.0 RR2 pKa = 11.84 SRR4 pKa = 11.84 ISNHH8 pKa = 5.46 GPGFRR13 pKa = 11.84 SFTTTSTIVAAFAAFAPEE31 pKa = 3.8 AHH33 pKa = 7.21 ADD35 pKa = 3.68 PTRR38 pKa = 11.84 LWGTYY43 pKa = 10.09 DD44 pKa = 3.48 GGSGTDD50 pKa = 2.87 AGRR53 pKa = 11.84 GVVIDD58 pKa = 4.56 DD59 pKa = 4.17 EE60 pKa = 5.23 FNVVCVGEE68 pKa = 4.56 TTSEE72 pKa = 3.86 TGITADD78 pKa = 3.6 AAHH81 pKa = 7.08 DD82 pKa = 3.65 GDD84 pKa = 4.6 YY85 pKa = 11.45 NGGRR89 pKa = 11.84 DD90 pKa = 3.55 AFITKK95 pKa = 9.93 FDD97 pKa = 3.56 WFGQQIWGNYY107 pKa = 7.98 YY108 pKa = 10.5 GGSGDD113 pKa = 5.79 DD114 pKa = 3.32 IFHH117 pKa = 7.26 DD118 pKa = 4.13 VVFDD122 pKa = 3.69 EE123 pKa = 5.45 AEE125 pKa = 4.09 GTFFAGGATRR135 pKa = 11.84 SANTGNVIATTGTHH149 pKa = 4.59 QTALDD154 pKa = 3.87 GTGDD158 pKa = 3.33 AMLVKK163 pKa = 10.33 FRR165 pKa = 11.84 NNGARR170 pKa = 11.84 LWGTYY175 pKa = 10.08 FGGDD179 pKa = 3.31 QLEE182 pKa = 4.37 EE183 pKa = 4.03 AFATCIDD190 pKa = 3.89 SQGDD194 pKa = 3.86 VYY196 pKa = 11.03 IVGTTTSEE204 pKa = 4.0 GLFDD208 pKa = 3.88 GMVGFTPWDD217 pKa = 3.86 DD218 pKa = 4.07 EE219 pKa = 4.58 LDD221 pKa = 3.71 GSIDD225 pKa = 3.42 AFIAKK230 pKa = 9.91 FDD232 pKa = 3.97 GTGGTLLWGTYY243 pKa = 9.56 YY244 pKa = 10.73 GGSGGEE250 pKa = 4.3 TKK252 pKa = 10.76 ALDD255 pKa = 3.86 CAVGSDD261 pKa = 3.39 DD262 pKa = 3.63 AVYY265 pKa = 9.15 VTGHH269 pKa = 4.98 TTSANNEE276 pKa = 3.98 LDD278 pKa = 3.32 GSHH281 pKa = 7.17 FDD283 pKa = 4.04 TYY285 pKa = 11.69 GGMQDD290 pKa = 3.54 AFLVRR295 pKa = 11.84 YY296 pKa = 9.38 DD297 pKa = 3.54 SSGVLEE303 pKa = 3.97 WATYY307 pKa = 10.44 YY308 pKa = 11.36 GDD310 pKa = 3.64 TGTDD314 pKa = 3.1 QARR317 pKa = 11.84 GVAVDD322 pKa = 3.59 VSGTDD327 pKa = 3.29 DD328 pKa = 3.36 LVYY331 pKa = 10.48 IVGLTDD337 pKa = 3.39 SDD339 pKa = 3.32 NDD341 pKa = 3.35 IAVNSTTLRR350 pKa = 11.84 AGSTDD355 pKa = 3.22 AFITQFDD362 pKa = 3.74 VDD364 pKa = 5.48 GDD366 pKa = 4.32 ILWGRR371 pKa = 11.84 YY372 pKa = 8.4 YY373 pKa = 11.03 GGAGLDD379 pKa = 3.37 QFEE382 pKa = 4.46 GVSAEE387 pKa = 4.11 GVIQASGATSSSGLFTSGFDD407 pKa = 3.3 NAYY410 pKa = 10.83 SGFGWDD416 pKa = 3.52 GMFVTFDD423 pKa = 3.68 ANGTAVWGSYY433 pKa = 10.39 NGGDD437 pKa = 3.64 GLDD440 pKa = 3.5 EE441 pKa = 4.19 SVIAVDD447 pKa = 3.84 RR448 pKa = 11.84 RR449 pKa = 11.84 YY450 pKa = 9.43 DD451 pKa = 3.46 TLVGVGSTTSTSNTGINDD469 pKa = 3.69 SNSPGTIHH477 pKa = 7.8 DD478 pKa = 3.49 PTYY481 pKa = 11.08 NGDD484 pKa = 3.37 YY485 pKa = 10.46 DD486 pKa = 3.96 IFMTLIRR493 pKa = 11.84 LFSTT497 pKa = 4.16
Molecular weight: 52.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.719
IPC_protein 3.783
Toseland 3.541
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.439
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.202
Thurlkill 3.592
EMBOSS 3.757
Sillero 3.91
Patrickios 0.973
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A1I2GC44|A0A1I2GC44_9DELT Undecaprenyl-diphosphatase OS=Nannocystis exedens OX=54 GN=uppP PE=3 SV=1
MM1 pKa = 7.59 ARR3 pKa = 11.84 PSATPRR9 pKa = 11.84 SRR11 pKa = 11.84 ILAHH15 pKa = 6.27 PRR17 pKa = 11.84 RR18 pKa = 11.84 ATWMVSRR25 pKa = 11.84 WRR27 pKa = 11.84 CFSSIARR34 pKa = 11.84 ASGNSSRR41 pKa = 11.84 GPTHH45 pKa = 6.79 HH46 pKa = 7.01 VRR48 pKa = 11.84 LATPWSLIASIARR61 pKa = 11.84 NSLAQPRR68 pKa = 11.84 HH69 pKa = 4.74 VRR71 pKa = 11.84 LATTAVASASIARR84 pKa = 11.84 RR85 pKa = 11.84 TRR87 pKa = 11.84 AVLSS91 pKa = 3.74
Molecular weight: 9.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.484
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.076
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.167
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9143
0
9143
3282201
39
4182
359.0
38.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.677 ± 0.045
1.383 ± 0.017
6.225 ± 0.022
6.022 ± 0.023
3.099 ± 0.015
9.029 ± 0.034
2.17 ± 0.013
3.351 ± 0.016
2.229 ± 0.022
10.406 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.68 ± 0.01
1.824 ± 0.014
6.556 ± 0.03
2.934 ± 0.014
8.045 ± 0.037
4.954 ± 0.018
5.399 ± 0.026
7.632 ± 0.022
1.423 ± 0.01
1.961 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here