Streptococcus phage Javan454

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B6X0|A0A4D6B6X0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan454 OX=2548178 GN=Javan454_0010 PE=4 SV=1
MM1 pKa = 7.16LTYY4 pKa = 10.89DD5 pKa = 4.11EE6 pKa = 5.19FKK8 pKa = 10.73QAIDD12 pKa = 3.44NGYY15 pKa = 7.78ITADD19 pKa = 2.79TVMIVRR25 pKa = 11.84KK26 pKa = 9.75NGQIFDD32 pKa = 3.78YY33 pKa = 10.46VLPHH37 pKa = 6.07EE38 pKa = 5.47EE39 pKa = 3.67IRR41 pKa = 11.84DD42 pKa = 3.74WEE44 pKa = 4.46VVTIEE49 pKa = 4.75RR50 pKa = 11.84ISDD53 pKa = 3.44VMAEE57 pKa = 4.11LSEE60 pKa = 4.54SEE62 pKa = 4.14

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B5H5|A0A4D6B5H5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan454 OX=2548178 GN=Javan454_0043 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.22KK7 pKa = 9.09KK8 pKa = 10.34RR9 pKa = 11.84KK10 pKa = 9.22LEE12 pKa = 4.06TAVVMLVAEE21 pKa = 4.41NAMQAEE27 pKa = 4.74AIKK30 pKa = 10.48NQNRR34 pKa = 11.84QIAEE38 pKa = 3.76LRR40 pKa = 11.84AIVQQNAQAANNEE53 pKa = 4.29LATVTGVCFDD63 pKa = 3.69NQAAIVDD70 pKa = 3.56IAIDD74 pKa = 3.39VNYY77 pKa = 10.04IKK79 pKa = 10.74KK80 pKa = 10.18NYY82 pKa = 7.73KK83 pKa = 10.03RR84 pKa = 11.84KK85 pKa = 8.79WGKK88 pKa = 9.76KK89 pKa = 7.11

Molecular weight: 10.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11569

37

999

199.5

22.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.967 ± 0.509

0.527 ± 0.099

6.37 ± 0.3

7.546 ± 0.422

3.942 ± 0.279

6.094 ± 0.408

1.521 ± 0.135

7.624 ± 0.359

9.387 ± 0.467

8.263 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.291 ± 0.186

5.489 ± 0.223

2.455 ± 0.164

4.106 ± 0.195

4.184 ± 0.277

5.982 ± 0.337

6.327 ± 0.269

5.93 ± 0.226

1.219 ± 0.177

3.777 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski