Amino acid dipepetide frequency for Streptococcus phage Javan454

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.68AlaAla: 2.68 ± 0.775
0.778AlaCys: 0.778 ± 0.289
5.187AlaAsp: 5.187 ± 0.742
6.397AlaGlu: 6.397 ± 0.709
3.112AlaPhe: 3.112 ± 0.531
5.187AlaGly: 5.187 ± 1.404
0.259AlaHis: 0.259 ± 0.14
6.483AlaIle: 6.483 ± 0.787
6.138AlaLys: 6.138 ± 0.876
6.397AlaLeu: 6.397 ± 1.356
2.248AlaMet: 2.248 ± 0.432
3.976AlaAsn: 3.976 ± 0.567
0.951AlaPro: 0.951 ± 0.279
2.507AlaGln: 2.507 ± 0.428
2.593AlaArg: 2.593 ± 0.357
2.939AlaSer: 2.939 ± 0.496
4.149AlaThr: 4.149 ± 0.597
3.631AlaVal: 3.631 ± 0.74
1.383AlaTrp: 1.383 ± 0.56
2.68AlaTyr: 2.68 ± 0.435
0.0AlaXaa: 0.0 ± 0.0
Cys
0.173CysAla: 0.173 ± 0.135
0.0CysCys: 0.0 ± 0.0
0.346CysAsp: 0.346 ± 0.166
0.519CysGlu: 0.519 ± 0.198
0.259CysPhe: 0.259 ± 0.178
0.173CysGly: 0.173 ± 0.122
0.0CysHis: 0.0 ± 0.0
0.605CysIle: 0.605 ± 0.228
0.346CysLys: 0.346 ± 0.148
0.346CysLeu: 0.346 ± 0.225
0.0CysMet: 0.0 ± 0.0
0.346CysAsn: 0.346 ± 0.201
0.173CysPro: 0.173 ± 0.117
0.346CysGln: 0.346 ± 0.161
0.432CysArg: 0.432 ± 0.269
0.346CysSer: 0.346 ± 0.166
0.346CysThr: 0.346 ± 0.165
0.259CysVal: 0.259 ± 0.133
0.259CysTrp: 0.259 ± 0.146
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.409AspAla: 4.409 ± 0.551
0.346AspCys: 0.346 ± 0.181
3.198AspAsp: 3.198 ± 0.574
4.495AspGlu: 4.495 ± 0.592
3.544AspPhe: 3.544 ± 0.609
4.582AspGly: 4.582 ± 0.708
0.951AspHis: 0.951 ± 0.253
5.273AspIle: 5.273 ± 0.778
5.36AspLys: 5.36 ± 0.885
5.273AspLeu: 5.273 ± 0.674
1.556AspMet: 1.556 ± 0.345
2.334AspAsn: 2.334 ± 0.414
1.383AspPro: 1.383 ± 0.282
1.037AspGln: 1.037 ± 0.313
3.026AspArg: 3.026 ± 0.398
3.631AspSer: 3.631 ± 0.595
4.495AspThr: 4.495 ± 0.469
4.495AspVal: 4.495 ± 0.642
0.519AspTrp: 0.519 ± 0.18
3.717AspTyr: 3.717 ± 0.568
0.0AspXaa: 0.0 ± 0.0
Glu
6.224GluAla: 6.224 ± 0.647
0.173GluCys: 0.173 ± 0.11
3.285GluAsp: 3.285 ± 0.527
4.582GluGlu: 4.582 ± 0.71
3.285GluPhe: 3.285 ± 0.487
3.976GluGly: 3.976 ± 0.615
1.383GluHis: 1.383 ± 0.323
6.483GluIle: 6.483 ± 0.698
6.656GluLys: 6.656 ± 1.013
8.558GluLeu: 8.558 ± 0.851
2.507GluMet: 2.507 ± 0.493
3.458GluAsn: 3.458 ± 0.681
1.642GluPro: 1.642 ± 0.407
2.68GluGln: 2.68 ± 0.498
3.544GluArg: 3.544 ± 0.731
4.236GluSer: 4.236 ± 0.626
4.236GluThr: 4.236 ± 0.45
4.582GluVal: 4.582 ± 0.632
0.951GluTrp: 0.951 ± 0.361
3.026GluTyr: 3.026 ± 0.433
0.0GluXaa: 0.0 ± 0.0
Phe
2.075PheAla: 2.075 ± 0.454
0.346PheCys: 0.346 ± 0.181
3.371PheAsp: 3.371 ± 0.502
2.939PheGlu: 2.939 ± 0.481
1.902PhePhe: 1.902 ± 0.572
2.248PheGly: 2.248 ± 0.418
0.692PheHis: 0.692 ± 0.227
2.853PheIle: 2.853 ± 0.426
4.409PheLys: 4.409 ± 0.753
3.112PheLeu: 3.112 ± 0.534
1.124PheMet: 1.124 ± 0.331
1.815PheAsn: 1.815 ± 0.381
0.605PhePro: 0.605 ± 0.221
0.778PheGln: 0.778 ± 0.294
1.815PheArg: 1.815 ± 0.401
2.161PheSer: 2.161 ± 0.708
2.593PheThr: 2.593 ± 0.415
2.766PheVal: 2.766 ± 0.475
0.173PheTrp: 0.173 ± 0.114
1.642PheTyr: 1.642 ± 0.373
0.0PheXaa: 0.0 ± 0.0
Gly
3.717GlyAla: 3.717 ± 0.524
0.259GlyCys: 0.259 ± 0.14
4.841GlyAsp: 4.841 ± 0.683
3.458GlyGlu: 3.458 ± 0.505
3.631GlyPhe: 3.631 ± 0.763
3.804GlyGly: 3.804 ± 0.707
1.297GlyHis: 1.297 ± 0.287
4.409GlyIle: 4.409 ± 0.854
6.483GlyLys: 6.483 ± 0.831
4.927GlyLeu: 4.927 ± 0.94
1.815GlyMet: 1.815 ± 0.359
3.112GlyAsn: 3.112 ± 0.525
0.519GlyPro: 0.519 ± 0.272
2.42GlyGln: 2.42 ± 0.366
2.68GlyArg: 2.68 ± 0.605
3.112GlySer: 3.112 ± 0.446
3.112GlyThr: 3.112 ± 0.645
3.631GlyVal: 3.631 ± 0.552
0.605GlyTrp: 0.605 ± 0.196
3.112GlyTyr: 3.112 ± 0.615
0.0GlyXaa: 0.0 ± 0.0
His
1.21HisAla: 1.21 ± 0.311
0.0HisCys: 0.0 ± 0.0
0.605HisAsp: 0.605 ± 0.23
1.556HisGlu: 1.556 ± 0.414
0.259HisPhe: 0.259 ± 0.168
0.951HisGly: 0.951 ± 0.325
0.346HisHis: 0.346 ± 0.187
1.21HisIle: 1.21 ± 0.416
1.556HisLys: 1.556 ± 0.306
1.21HisLeu: 1.21 ± 0.32
0.346HisMet: 0.346 ± 0.191
0.864HisAsn: 0.864 ± 0.221
0.432HisPro: 0.432 ± 0.196
0.778HisGln: 0.778 ± 0.224
0.432HisArg: 0.432 ± 0.171
0.692HisSer: 0.692 ± 0.308
0.692HisThr: 0.692 ± 0.265
1.124HisVal: 1.124 ± 0.305
0.259HisTrp: 0.259 ± 0.139
0.692HisTyr: 0.692 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
5.1IleAla: 5.1 ± 0.617
0.432IleCys: 0.432 ± 0.202
7.002IleAsp: 7.002 ± 1.019
5.792IleGlu: 5.792 ± 0.98
2.248IlePhe: 2.248 ± 0.404
5.1IleGly: 5.1 ± 0.843
1.297IleHis: 1.297 ± 0.359
5.1IleIle: 5.1 ± 0.736
8.645IleLys: 8.645 ± 1.238
4.754IleLeu: 4.754 ± 0.6
1.383IleMet: 1.383 ± 0.384
5.446IleAsn: 5.446 ± 0.646
1.642IlePro: 1.642 ± 0.402
2.593IleGln: 2.593 ± 0.478
3.285IleArg: 3.285 ± 0.609
3.717IleSer: 3.717 ± 0.764
5.619IleThr: 5.619 ± 0.742
3.89IleVal: 3.89 ± 0.664
1.21IleTrp: 1.21 ± 0.429
1.988IleTyr: 1.988 ± 0.478
0.0IleXaa: 0.0 ± 0.0
Lys
7.089LysAla: 7.089 ± 0.936
0.173LysCys: 0.173 ± 0.121
5.014LysAsp: 5.014 ± 0.635
6.224LysGlu: 6.224 ± 0.866
2.161LysPhe: 2.161 ± 0.489
4.841LysGly: 4.841 ± 0.901
1.383LysHis: 1.383 ± 0.33
7.78LysIle: 7.78 ± 0.752
7.175LysLys: 7.175 ± 1.089
7.694LysLeu: 7.694 ± 0.867
2.68LysMet: 2.68 ± 0.53
6.138LysAsn: 6.138 ± 0.63
3.112LysPro: 3.112 ± 0.446
4.754LysGln: 4.754 ± 0.614
3.717LysArg: 3.717 ± 0.693
6.224LysSer: 6.224 ± 0.722
7.261LysThr: 7.261 ± 0.885
5.965LysVal: 5.965 ± 0.933
1.037LysTrp: 1.037 ± 0.242
3.458LysTyr: 3.458 ± 0.568
0.0LysXaa: 0.0 ± 0.0
Leu
5.187LeuAla: 5.187 ± 1.009
0.519LeuCys: 0.519 ± 0.274
5.36LeuAsp: 5.36 ± 0.797
6.483LeuGlu: 6.483 ± 0.761
3.804LeuPhe: 3.804 ± 0.469
4.236LeuGly: 4.236 ± 0.805
0.692LeuHis: 0.692 ± 0.238
6.138LeuIle: 6.138 ± 0.628
9.855LeuLys: 9.855 ± 1.15
6.224LeuLeu: 6.224 ± 0.757
1.47LeuMet: 1.47 ± 0.331
5.187LeuAsn: 5.187 ± 0.595
2.507LeuPro: 2.507 ± 0.485
3.544LeuGln: 3.544 ± 0.648
3.026LeuArg: 3.026 ± 0.447
6.224LeuSer: 6.224 ± 0.663
5.792LeuThr: 5.792 ± 0.83
3.717LeuVal: 3.717 ± 0.484
0.951LeuTrp: 0.951 ± 0.329
1.729LeuTyr: 1.729 ± 0.383
0.0LeuXaa: 0.0 ± 0.0
Met
3.026MetAla: 3.026 ± 0.6
0.0MetCys: 0.0 ± 0.0
1.47MetAsp: 1.47 ± 0.273
1.21MetGlu: 1.21 ± 0.366
0.519MetPhe: 0.519 ± 0.219
0.778MetGly: 0.778 ± 0.241
0.259MetHis: 0.259 ± 0.151
1.556MetIle: 1.556 ± 0.306
1.47MetLys: 1.47 ± 0.352
1.902MetLeu: 1.902 ± 0.382
0.259MetMet: 0.259 ± 0.166
1.556MetAsn: 1.556 ± 0.362
1.037MetPro: 1.037 ± 0.283
1.47MetGln: 1.47 ± 0.307
1.47MetArg: 1.47 ± 0.348
1.124MetSer: 1.124 ± 0.365
1.642MetThr: 1.642 ± 0.382
1.037MetVal: 1.037 ± 0.399
0.432MetTrp: 0.432 ± 0.197
0.692MetTyr: 0.692 ± 0.247
0.0MetXaa: 0.0 ± 0.0
Asn
5.446AsnAla: 5.446 ± 1.016
0.346AsnCys: 0.346 ± 0.171
1.729AsnAsp: 1.729 ± 0.451
4.841AsnGlu: 4.841 ± 0.678
1.642AsnPhe: 1.642 ± 0.334
5.273AsnGly: 5.273 ± 0.641
1.124AsnHis: 1.124 ± 0.301
3.112AsnIle: 3.112 ± 0.493
4.582AsnLys: 4.582 ± 0.605
3.89AsnLeu: 3.89 ± 0.545
1.297AsnMet: 1.297 ± 0.35
3.458AsnAsn: 3.458 ± 0.722
1.902AsnPro: 1.902 ± 0.36
2.68AsnGln: 2.68 ± 0.526
2.248AsnArg: 2.248 ± 0.502
2.853AsnSer: 2.853 ± 0.554
3.026AsnThr: 3.026 ± 0.556
2.334AsnVal: 2.334 ± 0.542
0.951AsnTrp: 0.951 ± 0.337
2.161AsnTyr: 2.161 ± 0.422
0.0AsnXaa: 0.0 ± 0.0
Pro
0.951ProAla: 0.951 ± 0.304
0.0ProCys: 0.0 ± 0.0
1.729ProAsp: 1.729 ± 0.373
1.902ProGlu: 1.902 ± 0.496
1.037ProPhe: 1.037 ± 0.288
0.778ProGly: 0.778 ± 0.238
0.346ProHis: 0.346 ± 0.135
1.729ProIle: 1.729 ± 0.445
2.766ProLys: 2.766 ± 0.508
1.902ProLeu: 1.902 ± 0.356
0.173ProMet: 0.173 ± 0.129
1.47ProAsn: 1.47 ± 0.267
0.519ProPro: 0.519 ± 0.225
1.037ProGln: 1.037 ± 0.332
1.21ProArg: 1.21 ± 0.362
2.248ProSer: 2.248 ± 0.417
1.642ProThr: 1.642 ± 0.359
1.47ProVal: 1.47 ± 0.346
0.346ProTrp: 0.346 ± 0.181
1.297ProTyr: 1.297 ± 0.357
0.0ProXaa: 0.0 ± 0.0
Gln
3.89GlnAla: 3.89 ± 0.619
0.086GlnCys: 0.086 ± 0.075
1.902GlnAsp: 1.902 ± 0.368
2.507GlnGlu: 2.507 ± 0.42
1.21GlnPhe: 1.21 ± 0.282
2.507GlnGly: 2.507 ± 0.587
0.432GlnHis: 0.432 ± 0.187
3.458GlnIle: 3.458 ± 0.434
3.458GlnLys: 3.458 ± 0.521
3.804GlnLeu: 3.804 ± 0.638
0.519GlnMet: 0.519 ± 0.209
1.902GlnAsn: 1.902 ± 0.445
0.951GlnPro: 0.951 ± 0.312
2.507GlnGln: 2.507 ± 0.568
2.248GlnArg: 2.248 ± 0.471
3.717GlnSer: 3.717 ± 0.579
2.42GlnThr: 2.42 ± 0.535
2.075GlnVal: 2.075 ± 0.472
0.346GlnTrp: 0.346 ± 0.17
1.124GlnTyr: 1.124 ± 0.351
0.0GlnXaa: 0.0 ± 0.0
Arg
2.507ArgAla: 2.507 ± 0.483
0.173ArgCys: 0.173 ± 0.128
2.248ArgAsp: 2.248 ± 0.455
3.544ArgGlu: 3.544 ± 0.651
1.47ArgPhe: 1.47 ± 0.4
2.075ArgGly: 2.075 ± 0.399
0.951ArgHis: 0.951 ± 0.285
3.371ArgIle: 3.371 ± 0.763
4.495ArgLys: 4.495 ± 0.663
4.409ArgLeu: 4.409 ± 0.664
1.037ArgMet: 1.037 ± 0.299
1.729ArgAsn: 1.729 ± 0.415
0.864ArgPro: 0.864 ± 0.248
1.729ArgGln: 1.729 ± 0.402
2.248ArgArg: 2.248 ± 0.513
1.729ArgSer: 1.729 ± 0.355
1.815ArgThr: 1.815 ± 0.374
3.371ArgVal: 3.371 ± 0.529
0.259ArgTrp: 0.259 ± 0.153
1.815ArgTyr: 1.815 ± 0.416
0.0ArgXaa: 0.0 ± 0.0
Ser
3.89SerAla: 3.89 ± 0.606
0.259SerCys: 0.259 ± 0.141
4.236SerAsp: 4.236 ± 0.727
5.1SerGlu: 5.1 ± 0.697
2.853SerPhe: 2.853 ± 0.664
4.495SerGly: 4.495 ± 0.848
1.037SerHis: 1.037 ± 0.283
4.149SerIle: 4.149 ± 0.554
4.841SerLys: 4.841 ± 0.673
3.976SerLeu: 3.976 ± 0.678
1.556SerMet: 1.556 ± 0.33
3.544SerAsn: 3.544 ± 0.448
0.951SerPro: 0.951 ± 0.273
2.766SerGln: 2.766 ± 0.485
2.248SerArg: 2.248 ± 0.421
3.544SerSer: 3.544 ± 0.73
3.112SerThr: 3.112 ± 0.445
4.409SerVal: 4.409 ± 0.619
0.519SerTrp: 0.519 ± 0.179
2.334SerTyr: 2.334 ± 0.475
0.0SerXaa: 0.0 ± 0.0
Thr
5.533ThrAla: 5.533 ± 1.342
0.346ThrCys: 0.346 ± 0.178
4.495ThrAsp: 4.495 ± 0.44
3.717ThrGlu: 3.717 ± 0.643
1.815ThrPhe: 1.815 ± 0.41
3.804ThrGly: 3.804 ± 0.501
1.037ThrHis: 1.037 ± 0.318
4.754ThrIle: 4.754 ± 0.668
6.224ThrLys: 6.224 ± 1.156
5.1ThrLeu: 5.1 ± 0.631
0.778ThrMet: 0.778 ± 0.242
3.198ThrAsn: 3.198 ± 0.406
1.815ThrPro: 1.815 ± 0.372
2.853ThrGln: 2.853 ± 0.481
1.21ThrArg: 1.21 ± 0.345
4.495ThrSer: 4.495 ± 0.751
4.754ThrThr: 4.754 ± 0.775
3.804ThrVal: 3.804 ± 0.478
0.432ThrTrp: 0.432 ± 0.202
3.112ThrTyr: 3.112 ± 0.485
0.0ThrXaa: 0.0 ± 0.0
Val
3.631ValAla: 3.631 ± 0.63
0.605ValCys: 0.605 ± 0.217
3.631ValAsp: 3.631 ± 0.602
5.446ValGlu: 5.446 ± 0.675
2.42ValPhe: 2.42 ± 0.386
3.112ValGly: 3.112 ± 0.604
0.864ValHis: 0.864 ± 0.294
4.841ValIle: 4.841 ± 0.631
5.273ValLys: 5.273 ± 0.731
4.495ValLeu: 4.495 ± 0.567
1.383ValMet: 1.383 ± 0.39
2.507ValAsn: 2.507 ± 0.393
1.988ValPro: 1.988 ± 0.446
1.902ValGln: 1.902 ± 0.41
2.507ValArg: 2.507 ± 0.508
4.063ValSer: 4.063 ± 0.442
3.976ValThr: 3.976 ± 0.502
4.409ValVal: 4.409 ± 0.522
1.124ValTrp: 1.124 ± 0.489
1.124ValTyr: 1.124 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
1.037TrpAla: 1.037 ± 0.328
0.173TrpCys: 0.173 ± 0.13
0.692TrpAsp: 0.692 ± 0.282
1.642TrpGlu: 1.642 ± 0.741
0.692TrpPhe: 0.692 ± 0.235
0.778TrpGly: 0.778 ± 0.256
0.086TrpHis: 0.086 ± 0.079
0.432TrpIle: 0.432 ± 0.244
0.951TrpLys: 0.951 ± 0.266
1.21TrpLeu: 1.21 ± 0.425
0.086TrpMet: 0.086 ± 0.091
0.864TrpAsn: 0.864 ± 0.422
0.259TrpPro: 0.259 ± 0.146
0.692TrpGln: 0.692 ± 0.228
0.692TrpArg: 0.692 ± 0.209
0.605TrpSer: 0.605 ± 0.183
0.346TrpThr: 0.346 ± 0.195
0.346TrpVal: 0.346 ± 0.135
0.259TrpTrp: 0.259 ± 0.133
0.346TrpTyr: 0.346 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.902TyrAla: 1.902 ± 0.405
0.259TyrCys: 0.259 ± 0.135
3.371TyrAsp: 3.371 ± 0.417
3.112TyrGlu: 3.112 ± 0.469
1.556TyrPhe: 1.556 ± 0.458
2.248TyrGly: 2.248 ± 0.402
0.778TyrHis: 0.778 ± 0.275
2.507TyrIle: 2.507 ± 0.41
3.198TyrLys: 3.198 ± 0.631
3.458TyrLeu: 3.458 ± 0.614
0.605TyrMet: 0.605 ± 0.179
1.988TyrAsn: 1.988 ± 0.429
1.297TyrPro: 1.297 ± 0.398
1.988TyrGln: 1.988 ± 0.48
1.21TyrArg: 1.21 ± 0.312
2.161TyrSer: 2.161 ± 0.43
2.248TyrThr: 2.248 ± 0.448
1.988TyrVal: 1.988 ± 0.389
0.173TyrTrp: 0.173 ± 0.121
1.729TyrTyr: 1.729 ± 0.478
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (11569 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski