Aeromonas phage 2_L372X
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 211 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B9N729|A0A5B9N729_9CAUD Baseplate_J domain-containing protein OS=Aeromonas phage 2_L372X OX=2588515 GN=2L372X_079 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.81 VSKK5 pKa = 9.74 MIKK8 pKa = 9.87 LLQQMPQDD16 pKa = 3.84 LEE18 pKa = 4.46 VYY20 pKa = 9.63 SYY22 pKa = 10.8 CDD24 pKa = 2.93 HH25 pKa = 6.4 GQTPEE30 pKa = 4.17 SSCAPQVVVVVKK42 pKa = 10.46 SEE44 pKa = 3.98 YY45 pKa = 10.6 DD46 pKa = 2.91 RR47 pKa = 11.84 GSIDD51 pKa = 4.13 GYY53 pKa = 8.25 MEE55 pKa = 4.83 KK56 pKa = 10.61 DD57 pKa = 3.35 DD58 pKa = 6.1 AIDD61 pKa = 3.31 SGYY64 pKa = 10.88 EE65 pKa = 3.8 EE66 pKa = 4.8 EE67 pKa = 4.9 DD68 pKa = 3.28 LVEE71 pKa = 4.74 IVLLL75 pKa = 4.18
Molecular weight: 8.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.853
IPC2_protein 4.126
IPC_protein 4.024
Toseland 3.846
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.91
Rodwell 3.859
Grimsley 3.757
Solomon 3.986
Lehninger 3.948
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.884
EMBOSS 3.923
Sillero 4.139
Patrickios 1.939
IPC_peptide 3.986
IPC2_peptide 4.126
IPC2.peptide.svr19 4.04
Protein with the highest isoelectric point:
>tr|A0A5B9N388|A0A5B9N388_9CAUD Uncharacterized protein OS=Aeromonas phage 2_L372X OX=2588515 GN=2L372X_041 PE=4 SV=1
MM1 pKa = 6.79 SHH3 pKa = 6.96 WYY5 pKa = 8.26 IQNGFFIVQVFGYY18 pKa = 9.09 RR19 pKa = 11.84 FEE21 pKa = 4.83 VIDD24 pKa = 3.78 RR25 pKa = 11.84 KK26 pKa = 10.52 RR27 pKa = 11.84 NPVLSTTVFPCYY39 pKa = 9.88 GSKK42 pKa = 10.25 EE43 pKa = 3.89 FRR45 pKa = 11.84 IWKK48 pKa = 8.79 YY49 pKa = 9.71 AVKK52 pKa = 10.28 FGKK55 pKa = 9.97 VV56 pKa = 2.84
Molecular weight: 6.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.2
IPC2_protein 9.531
IPC_protein 9.633
Toseland 9.97
ProMoST 9.721
Dawson 10.218
Bjellqvist 9.911
Wikipedia 10.409
Rodwell 10.57
Grimsley 10.306
Solomon 10.262
Lehninger 10.218
Nozaki 9.984
DTASelect 9.911
Thurlkill 10.043
EMBOSS 10.379
Sillero 10.131
Patrickios 10.248
IPC_peptide 10.248
IPC2_peptide 8.726
IPC2.peptide.svr19 8.39
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
211
0
211
34578
29
757
163.9
18.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.073 ± 0.252
1.787 ± 0.119
6.472 ± 0.165
7.47 ± 0.218
4.665 ± 0.139
6.059 ± 0.258
1.819 ± 0.093
6.626 ± 0.154
8.705 ± 0.289
7.889 ± 0.201
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.105
5.755 ± 0.12
2.941 ± 0.113
3.849 ± 0.125
3.65 ± 0.13
6.559 ± 0.174
5.261 ± 0.165
6.666 ± 0.16
1.371 ± 0.088
4.789 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here