Amino acid dipepetide frequency for Bacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.615AlaAla: 4.615 ± 0.095	0.535AlaCys: 0.535 ± 0.022	2.899AlaAsp: 2.899 ± 0.05	4.171AlaGlu: 4.171 ± 0.073	3.103AlaPhe: 3.103 ± 0.061	4.322AlaGly: 4.322 ± 0.073	1.243AlaHis: 1.243 ± 0.031	5.925AlaIle: 5.925 ± 0.079	4.072AlaLys: 4.072 ± 0.069	6.562AlaLeu: 6.562 ± 0.085	1.864AlaMet: 1.864 ± 0.044	2.852AlaAsn: 2.852 ± 0.053	1.883AlaPro: 1.883 ± 0.043	1.923AlaGln: 1.923 ± 0.043	2.393AlaArg: 2.393 ± 0.044	3.762AlaSer: 3.762 ± 0.056	3.526AlaThr: 3.526 ± 0.058	4.543AlaVal: 4.543 ± 0.065	0.603AlaTrp: 0.603 ± 0.024	2.184AlaTyr: 2.184 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.389CysAla: 0.389 ± 0.02	0.109CysCys: 0.109 ± 0.009	0.388CysAsp: 0.388 ± 0.019	0.446CysGlu: 0.446 ± 0.021	0.327CysPhe: 0.327 ± 0.014	0.644CysGly: 0.644 ± 0.026	0.196CysHis: 0.196 ± 0.015	0.517CysIle: 0.517 ± 0.018	0.42CysLys: 0.42 ± 0.019	0.639CysLeu: 0.639 ± 0.023	0.17CysMet: 0.17 ± 0.012	0.333CysAsn: 0.333 ± 0.017	0.329CysPro: 0.329 ± 0.018	0.212CysGln: 0.212 ± 0.012	0.277CysArg: 0.277 ± 0.014	0.514CysSer: 0.514 ± 0.021	0.381CysThr: 0.381 ± 0.021	0.372CysVal: 0.372 ± 0.017	0.075CysTrp: 0.075 ± 0.009	0.265CysTyr: 0.265 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.267AspAla: 3.267 ± 0.06	0.348AspCys: 0.348 ± 0.016	3.126AspAsp: 3.126 ± 0.06	4.964AspGlu: 4.964 ± 0.079	2.455AspPhe: 2.455 ± 0.043	3.634AspGly: 3.634 ± 0.065	1.274AspHis: 1.274 ± 0.032	4.479AspIle: 4.479 ± 0.058	3.11AspLys: 3.11 ± 0.06	4.809AspLeu: 4.809 ± 0.058	1.438AspMet: 1.438 ± 0.037	2.297AspAsn: 2.297 ± 0.042	2.007AspPro: 2.007 ± 0.052	1.797AspGln: 1.797 ± 0.04	2.312AspArg: 2.312 ± 0.047	2.755AspSer: 2.755 ± 0.05	2.604AspThr: 2.604 ± 0.054	4.035AspVal: 4.035 ± 0.065	0.714AspTrp: 0.714 ± 0.031	2.275AspTyr: 2.275 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	5.321GluAla: 5.321 ± 0.089	0.42GluCys: 0.42 ± 0.019	4.409GluAsp: 4.409 ± 0.082	8.295GluGlu: 8.295 ± 0.117	2.461GluPhe: 2.461 ± 0.043	4.631GluGly: 4.631 ± 0.075	1.628GluHis: 1.628 ± 0.035	5.726GluIle: 5.726 ± 0.08	6.824GluLys: 6.824 ± 0.093	7.412GluLeu: 7.412 ± 0.081	2.388GluMet: 2.388 ± 0.052	4.2GluAsn: 4.2 ± 0.067	2.002GluPro: 2.002 ± 0.047	3.316GluGln: 3.316 ± 0.054	3.74GluArg: 3.74 ± 0.054	3.797GluSer: 3.797 ± 0.067	3.949GluThr: 3.949 ± 0.059	5.732GluVal: 5.732 ± 0.076	0.957GluTrp: 0.957 ± 0.027	2.452GluTyr: 2.452 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	2.698PheAla: 2.698 ± 0.054	0.34PheCys: 0.34 ± 0.017	2.515PheAsp: 2.515 ± 0.046	3.05PheGlu: 3.05 ± 0.049	2.618PhePhe: 2.618 ± 0.067	3.14PheGly: 3.14 ± 0.072	1.195PheHis: 1.195 ± 0.028	4.408PheIle: 4.408 ± 0.083	2.387PheLys: 2.387 ± 0.047	4.512PheLeu: 4.512 ± 0.085	1.169PheMet: 1.169 ± 0.029	2.075PheAsn: 2.075 ± 0.046	1.62PhePro: 1.62 ± 0.033	1.678PheGln: 1.678 ± 0.041	1.647PheArg: 1.647 ± 0.038	3.539PheSer: 3.539 ± 0.057	2.679PheThr: 2.679 ± 0.048	3.173PheVal: 3.173 ± 0.064	0.491PheTrp: 0.491 ± 0.021	1.871PheTyr: 1.871 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.395GlyAla: 4.395 ± 0.07	0.548GlyCys: 0.548 ± 0.026	3.495GlyAsp: 3.495 ± 0.064	4.895GlyGlu: 4.895 ± 0.083	3.196GlyPhe: 3.196 ± 0.06	4.489GlyGly: 4.489 ± 0.091	1.391GlyHis: 1.391 ± 0.035	5.516GlyIle: 5.516 ± 0.071	4.415GlyLys: 4.415 ± 0.066	5.874GlyLeu: 5.874 ± 0.079	1.947GlyMet: 1.947 ± 0.04	2.913GlyAsn: 2.913 ± 0.054	1.623GlyPro: 1.623 ± 0.042	1.989GlyGln: 1.989 ± 0.048	2.484GlyArg: 2.484 ± 0.05	3.751GlySer: 3.751 ± 0.071	3.748GlyThr: 3.748 ± 0.06	4.879GlyVal: 4.879 ± 0.073	0.778GlyTrp: 0.778 ± 0.028	2.763GlyTyr: 2.763 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.258HisAla: 1.258 ± 0.029	0.196HisCys: 0.196 ± 0.012	1.179HisAsp: 1.179 ± 0.031	1.594HisGlu: 1.594 ± 0.039	1.242HisPhe: 1.242 ± 0.035	1.467HisGly: 1.467 ± 0.039	0.81HisHis: 0.81 ± 0.028	1.735HisIle: 1.735 ± 0.04	1.061HisLys: 1.061 ± 0.032	2.317HisLeu: 2.317 ± 0.047	0.548HisMet: 0.548 ± 0.024	0.836HisAsn: 0.836 ± 0.026	1.062HisPro: 1.062 ± 0.031	0.834HisGln: 0.834 ± 0.026	0.918HisArg: 0.918 ± 0.03	1.365HisSer: 1.365 ± 0.041	1.11HisThr: 1.11 ± 0.029	1.629HisVal: 1.629 ± 0.037	0.261HisTrp: 0.261 ± 0.015	0.955HisTyr: 0.955 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.687IleAla: 5.687 ± 0.077	0.635IleCys: 0.635 ± 0.026	4.816IleAsp: 4.816 ± 0.066	6.315IleGlu: 6.315 ± 0.087	3.698IlePhe: 3.698 ± 0.068	5.836IleGly: 5.836 ± 0.082	1.819IleHis: 1.819 ± 0.038	6.603IleIle: 6.603 ± 0.094	4.711IleLys: 4.711 ± 0.07	7.092IleLeu: 7.092 ± 0.101	1.786IleMet: 1.786 ± 0.036	3.979IleAsn: 3.979 ± 0.064	3.35IlePro: 3.35 ± 0.049	2.753IleGln: 2.753 ± 0.046	3.059IleArg: 3.059 ± 0.057	5.395IleSer: 5.395 ± 0.068	4.627IleThr: 4.627 ± 0.057	5.922IleVal: 5.922 ± 0.08	0.721IleTrp: 0.721 ± 0.026	2.826IleTyr: 2.826 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.02LysAla: 4.02 ± 0.069	0.379LysCys: 0.379 ± 0.019	3.627LysAsp: 3.627 ± 0.054	6.878LysGlu: 6.878 ± 0.1	1.827LysPhe: 1.827 ± 0.037	4.171LysGly: 4.171 ± 0.066	1.49LysHis: 1.49 ± 0.039	4.506LysIle: 4.506 ± 0.066	6.021LysLys: 6.021 ± 0.089	5.836LysLeu: 5.836 ± 0.086	2.041LysMet: 2.041 ± 0.043	3.551LysAsn: 3.551 ± 0.058	1.87LysPro: 1.87 ± 0.04	3.003LysGln: 3.003 ± 0.056	3.412LysArg: 3.412 ± 0.056	3.481LysSer: 3.481 ± 0.051	3.429LysThr: 3.429 ± 0.05	4.672LysVal: 4.672 ± 0.068	0.795LysTrp: 0.795 ± 0.028	2.299LysTyr: 2.299 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	6.196LeuAla: 6.196 ± 0.082	0.68LeuCys: 0.68 ± 0.022	4.837LeuAsp: 4.837 ± 0.064	6.71LeuGlu: 6.71 ± 0.084	5.011LeuPhe: 5.011 ± 0.087	5.885LeuGly: 5.885 ± 0.071	2.13LeuHis: 2.13 ± 0.043	7.375LeuIle: 7.375 ± 0.099	6.41LeuLys: 6.41 ± 0.08	10.158LeuLeu: 10.158 ± 0.127	2.509LeuMet: 2.509 ± 0.048	4.364LeuAsn: 4.364 ± 0.059	3.732LeuPro: 3.732 ± 0.057	3.518LeuGln: 3.518 ± 0.058	3.576LeuArg: 3.576 ± 0.061	6.782LeuSer: 6.782 ± 0.076	5.418LeuThr: 5.418 ± 0.069	6.086LeuVal: 6.086 ± 0.084	0.827LeuTrp: 0.827 ± 0.025	3.351LeuTyr: 3.351 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	1.804MetAla: 1.804 ± 0.044	0.146MetCys: 0.146 ± 0.011	1.458MetAsp: 1.458 ± 0.03	2.052MetGlu: 2.052 ± 0.048	1.137MetPhe: 1.137 ± 0.035	1.55MetGly: 1.55 ± 0.036	0.491MetHis: 0.491 ± 0.018	2.29MetIle: 2.29 ± 0.049	2.453MetLys: 2.453 ± 0.045	2.449MetLeu: 2.449 ± 0.04	0.899MetMet: 0.899 ± 0.032	1.641MetAsn: 1.641 ± 0.035	0.863MetPro: 0.863 ± 0.027	0.824MetGln: 0.824 ± 0.025	1.091MetArg: 1.091 ± 0.033	1.748MetSer: 1.748 ± 0.035	1.697MetThr: 1.697 ± 0.038	1.631MetVal: 1.631 ± 0.035	0.201MetTrp: 0.201 ± 0.012	0.816MetTyr: 0.816 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.669AsnAla: 2.669 ± 0.049	0.302AsnCys: 0.302 ± 0.018	2.866AsnAsp: 2.866 ± 0.053	4.372AsnGlu: 4.372 ± 0.067	1.778AsnPhe: 1.778 ± 0.042	3.43AsnGly: 3.43 ± 0.06	1.153AsnHis: 1.153 ± 0.036	4.093AsnIle: 4.093 ± 0.057	3.429AsnLys: 3.429 ± 0.052	3.705AsnLeu: 3.705 ± 0.061	1.325AsnMet: 1.325 ± 0.033	2.709AsnAsn: 2.709 ± 0.054	1.864AsnPro: 1.864 ± 0.041	1.74AsnGln: 1.74 ± 0.039	2.137AsnArg: 2.137 ± 0.043	2.475AsnSer: 2.475 ± 0.043	2.467AsnThr: 2.467 ± 0.051	3.473AsnVal: 3.473 ± 0.052	0.563AsnTrp: 0.563 ± 0.027	1.797AsnTyr: 1.797 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	1.828ProAla: 1.828 ± 0.042	0.212ProCys: 0.212 ± 0.016	1.828ProAsp: 1.828 ± 0.044	2.714ProGlu: 2.714 ± 0.055	1.969ProPhe: 1.969 ± 0.042	2.14ProGly: 2.14 ± 0.058	0.857ProHis: 0.857 ± 0.025	2.945ProIle: 2.945 ± 0.053	1.94ProLys: 1.94 ± 0.047	3.311ProLeu: 3.311 ± 0.048	0.83ProMet: 0.83 ± 0.03	1.66ProAsn: 1.66 ± 0.034	1.001ProPro: 1.001 ± 0.033	1.084ProGln: 1.084 ± 0.033	1.029ProArg: 1.029 ± 0.03	2.243ProSer: 2.243 ± 0.048	1.952ProThr: 1.952 ± 0.042	2.472ProVal: 2.472 ± 0.045	0.354ProTrp: 0.354 ± 0.016	1.382ProTyr: 1.382 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	2.238GlnAla: 2.238 ± 0.043	0.207GlnCys: 0.207 ± 0.012	1.505GlnAsp: 1.505 ± 0.035	2.767GlnGlu: 2.767 ± 0.054	1.73GlnPhe: 1.73 ± 0.036	2.017GlnGly: 2.017 ± 0.039	0.836GlnHis: 0.836 ± 0.026	2.477GlnIle: 2.477 ± 0.04	2.331GlnLys: 2.331 ± 0.041	4.138GlnLeu: 4.138 ± 0.065	1.103GlnMet: 1.103 ± 0.027	1.531GlnAsn: 1.531 ± 0.034	1.053GlnPro: 1.053 ± 0.034	1.809GlnGln: 1.809 ± 0.047	1.508GlnArg: 1.508 ± 0.038	2.161GlnSer: 2.161 ± 0.042	1.687GlnThr: 1.687 ± 0.039	2.273GlnVal: 2.273 ± 0.045	0.424GlnTrp: 0.424 ± 0.019	1.423GlnTyr: 1.423 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.374ArgAla: 2.374 ± 0.045	0.255ArgCys: 0.255 ± 0.014	2.121ArgAsp: 2.121 ± 0.046	3.335ArgGlu: 3.335 ± 0.05	1.942ArgPhe: 1.942 ± 0.041	2.384ArgGly: 2.384 ± 0.046	0.808ArgHis: 0.808 ± 0.026	3.032ArgIle: 3.032 ± 0.052	3.177ArgLys: 3.177 ± 0.058	3.875ArgLeu: 3.875 ± 0.06	1.227ArgMet: 1.227 ± 0.027	2.117ArgAsn: 2.117 ± 0.039	1.279ArgPro: 1.279 ± 0.034	1.468ArgGln: 1.468 ± 0.038	1.867ArgArg: 1.867 ± 0.044	2.248ArgSer: 2.248 ± 0.042	2.056ArgThr: 2.056 ± 0.04	2.642ArgVal: 2.642 ± 0.052	0.505ArgTrp: 0.505 ± 0.021	1.528ArgTyr: 1.528 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.337SerAla: 3.337 ± 0.059	0.4SerCys: 0.4 ± 0.019	3.128SerAsp: 3.128 ± 0.05	4.312SerGlu: 4.312 ± 0.072	3.609SerPhe: 3.609 ± 0.065	3.948SerGly: 3.948 ± 0.072	1.302SerHis: 1.302 ± 0.032	5.49SerIle: 5.49 ± 0.072	3.87SerLys: 3.87 ± 0.055	6.244SerLeu: 6.244 ± 0.075	1.67SerMet: 1.67 ± 0.04	2.943SerAsn: 2.943 ± 0.055	2.022SerPro: 2.022 ± 0.041	1.957SerGln: 1.957 ± 0.037	2.192SerArg: 2.192 ± 0.038	4.008SerSer: 4.008 ± 0.064	3.327SerThr: 3.327 ± 0.046	4.171SerVal: 4.171 ± 0.062	0.688SerTrp: 0.688 ± 0.024	2.326SerTyr: 2.326 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.522ThrAla: 3.522 ± 0.06	0.331ThrCys: 0.331 ± 0.016	2.705ThrAsp: 2.705 ± 0.054	3.703ThrGlu: 3.703 ± 0.057	2.899ThrPhe: 2.899 ± 0.057	3.759ThrGly: 3.759 ± 0.06	1.093ThrHis: 1.093 ± 0.028	4.866ThrIle: 4.866 ± 0.067	3.309ThrLys: 3.309 ± 0.059	5.36ThrLeu: 5.36 ± 0.063	1.336ThrMet: 1.336 ± 0.033	2.82ThrAsn: 2.82 ± 0.056	2.134ThrPro: 2.134 ± 0.048	1.425ThrGln: 1.425 ± 0.04	1.886ThrArg: 1.886 ± 0.037	3.326ThrSer: 3.326 ± 0.05	3.12ThrThr: 3.12 ± 0.062	4.141ThrVal: 4.141 ± 0.064	0.574ThrTrp: 0.574 ± 0.021	2.113ThrTyr: 2.113 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	4.726ValAla: 4.726 ± 0.08	0.547ValCys: 0.547 ± 0.021	3.991ValAsp: 3.991 ± 0.057	5.282ValGlu: 5.282 ± 0.071	3.276ValPhe: 3.276 ± 0.066	4.507ValGly: 4.507 ± 0.074	1.421ValHis: 1.421 ± 0.033	5.912ValIle: 5.912 ± 0.087	4.559ValLys: 4.559 ± 0.064	6.576ValLeu: 6.576 ± 0.073	1.793ValMet: 1.793 ± 0.035	3.321ValAsn: 3.321 ± 0.056	2.43ValPro: 2.43 ± 0.043	2.228ValGln: 2.228 ± 0.043	2.639ValArg: 2.639 ± 0.049	4.638ValSer: 4.638 ± 0.068	4.232ValThr: 4.232 ± 0.059	5.064ValVal: 5.064 ± 0.079	0.615ValTrp: 0.615 ± 0.022	2.303ValTyr: 2.303 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.589TrpAla: 0.589 ± 0.022	0.087TrpCys: 0.087 ± 0.008	0.609TrpAsp: 0.609 ± 0.022	0.762TrpGlu: 0.762 ± 0.026	0.547TrpPhe: 0.547 ± 0.02	0.702TrpGly: 0.702 ± 0.026	0.249TrpHis: 0.249 ± 0.015	0.923TrpIle: 0.923 ± 0.031	0.756TrpLys: 0.756 ± 0.026	1.157TrpLeu: 1.157 ± 0.032	0.346TrpMet: 0.346 ± 0.018	0.574TrpAsn: 0.574 ± 0.022	0.285TrpPro: 0.285 ± 0.019	0.373TrpGln: 0.373 ± 0.018	0.426TrpArg: 0.426 ± 0.019	0.668TrpSer: 0.668 ± 0.023	0.507TrpThr: 0.507 ± 0.023	0.656TrpVal: 0.656 ± 0.023	0.166TrpTrp: 0.166 ± 0.014	0.367TrpTyr: 0.367 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.048TyrAla: 2.048 ± 0.038	0.323TyrCys: 0.323 ± 0.018	2.198TyrAsp: 2.198 ± 0.05	3.043TyrGlu: 3.043 ± 0.057	1.978TyrPhe: 1.978 ± 0.049	2.436TyrGly: 2.436 ± 0.043	0.989TyrHis: 0.989 ± 0.029	2.813TyrIle: 2.813 ± 0.046	2.058TyrLys: 2.058 ± 0.043	3.53TyrLeu: 3.53 ± 0.062	0.853TyrMet: 0.853 ± 0.027	1.63TyrAsn: 1.63 ± 0.04	1.344TyrPro: 1.344 ± 0.037	1.288TyrGln: 1.288 ± 0.033	1.644TyrArg: 1.644 ± 0.036	2.289TyrSer: 2.289 ± 0.042	1.904TyrThr: 1.904 ± 0.041	2.492TyrVal: 2.492 ± 0.045	0.437TyrTrp: 0.437 ± 0.019	1.633TyrTyr: 1.633 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4253 proteins (1243458 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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