Roseovarius indicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5534 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T5P4M9|A0A0T5P4M9_9RHOB Excisionase OS=Roseovarius indicus OX=540747 GN=RIdsm_00738 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.27KK3 pKa = 9.89QLLCTSAIALGIAAAPAAAQEE24 pKa = 4.33WNLDD28 pKa = 2.75WGGYY32 pKa = 6.75MNQHH36 pKa = 5.56VAFGDD41 pKa = 3.25ISDD44 pKa = 4.52NLTALTGLASPDD56 pKa = 3.36WEE58 pKa = 5.25GDD60 pKa = 3.48GLDD63 pKa = 3.55LHH65 pKa = 5.89QTTEE69 pKa = 4.15IIFTPSVTLDD79 pKa = 2.8NGMTFGINVQLEE91 pKa = 4.53GEE93 pKa = 4.13RR94 pKa = 11.84TAANIDD100 pKa = 3.7EE101 pKa = 4.87TYY103 pKa = 10.41MSISSDD109 pKa = 3.08TFGRR113 pKa = 11.84LDD115 pKa = 3.66LGAEE119 pKa = 3.77NSAGYY124 pKa = 10.42KK125 pKa = 10.52SMVGAPGVTSMYY137 pKa = 9.99INSPSTSAFIPYY149 pKa = 9.12TGVDD153 pKa = 3.49AFLSAAAGTPITLVTLPFRR172 pKa = 11.84QAGLSSYY179 pKa = 10.58TEE181 pKa = 3.74VGGNNDD187 pKa = 3.26VQRR190 pKa = 11.84ITYY193 pKa = 7.13YY194 pKa = 10.54TPDD197 pKa = 3.57FNGLTVGVSYY207 pKa = 11.0APQGVVNAASSFSINKK223 pKa = 9.13NAVLSDD229 pKa = 3.09IFDD232 pKa = 3.45IGVNYY237 pKa = 10.7SNTFGSTDD245 pKa = 2.75ITLAARR251 pKa = 11.84WGTGDD256 pKa = 3.9SPSTAPTASDD266 pKa = 3.76FEE268 pKa = 4.5TWGVGFQVGFNNITVGGSWTEE289 pKa = 3.68NDD291 pKa = 3.23NDD293 pKa = 3.68EE294 pKa = 4.45TLVPSVIGIGDD305 pKa = 3.66SEE307 pKa = 4.33GWSFGVTYY315 pKa = 10.59DD316 pKa = 4.97AAGPWAFEE324 pKa = 3.53ALTYY328 pKa = 10.48QSEE331 pKa = 4.3TDD333 pKa = 3.75LVVGGVALGKK343 pKa = 8.59TDD345 pKa = 3.56RR346 pKa = 11.84EE347 pKa = 4.42AYY349 pKa = 10.21RR350 pKa = 11.84IGASRR355 pKa = 11.84TLGPGVDD362 pKa = 2.8WDD364 pKa = 3.56IYY366 pKa = 9.98VVHH369 pKa = 6.12EE370 pKa = 4.31TRR372 pKa = 11.84DD373 pKa = 3.8ANILGALPGDD383 pKa = 3.91EE384 pKa = 4.97VEE386 pKa = 4.46GTLLGTAINLSFF398 pKa = 4.58

Molecular weight: 41.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T5PBJ0|A0A0T5PBJ0_9RHOB Peptidase_S9 domain-containing protein OS=Roseovarius indicus OX=540747 GN=XM52_06620 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5534

0

5534

1712709

35

2708

309.5

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.924 ± 0.044

0.956 ± 0.01

6.067 ± 0.027

6.451 ± 0.035

3.715 ± 0.021

8.846 ± 0.03

2.097 ± 0.018

5.041 ± 0.022

3.156 ± 0.026

9.88 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.018

2.53 ± 0.02

5.089 ± 0.022

3.024 ± 0.018

6.721 ± 0.032

5.103 ± 0.019

5.517 ± 0.024

7.327 ± 0.03

1.427 ± 0.016

2.292 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski