Nonlabens sp. MB-3u-79
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2710 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K8X8N8|A0A2K8X8N8_9FLAO DUF2179 domain-containing protein OS=Nonlabens sp. MB-3u-79 OX=2058134 GN=CW736_07850 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.41 KK3 pKa = 10.47 LYY5 pKa = 9.71 TILAALFLTASAFAQAPEE23 pKa = 4.04 KK24 pKa = 10.07 MSYY27 pKa = 9.19 QAVVRR32 pKa = 11.84 DD33 pKa = 3.69 SGDD36 pKa = 3.42 ALVTSQPVGMQISILQTTATGTAVYY61 pKa = 10.71 VEE63 pKa = 4.59 TQTPTTNVNGLVSLEE78 pKa = 4.09 IGTGSVVSGDD88 pKa = 3.49 FTSIDD93 pKa = 3.55 WSTDD97 pKa = 2.5 SYY99 pKa = 11.09 FIKK102 pKa = 10.12 TEE104 pKa = 3.84 TDD106 pKa = 3.15 PAGGTTYY113 pKa = 10.35 TITGTSQLMSVPFALYY129 pKa = 10.65 AKK131 pKa = 9.6 TSGNGAGPIGPQGPAGNDD149 pKa = 3.51 GATGPIGADD158 pKa = 3.26 GAAGTNGTDD167 pKa = 3.46 GAQGIQGAQGDD178 pKa = 4.03 IGAAGADD185 pKa = 3.91 GAQGIQGADD194 pKa = 3.25 GATGTTGQQGPAGNDD209 pKa = 3.37 GATGQQGLAGNDD221 pKa = 3.5 GATGLTGPVGPTGPAGSLGPQGIQGAAGINGTPGIQGAPGTAGAPGVDD269 pKa = 3.8 GAAGTNGTDD278 pKa = 3.46 GAQGIQGADD287 pKa = 3.25 GATGTTGQQGPAGNDD302 pKa = 3.21 GTTGQQGLAGNDD314 pKa = 3.5 GATGLTGLVGPAGSLGPQGIQGAAGINGTPGIQGATGASGADD356 pKa = 3.7 GAAGAPGTAGAPGVDD371 pKa = 3.8 GAAGTNGIDD380 pKa = 3.54 GTQGIQGATGADD392 pKa = 4.05 GATGPSGTNGTQGIQGATGADD413 pKa = 3.8 GAQGIQGATGADD425 pKa = 3.8 GAQGIQGEE433 pKa = 4.46 TGAAGTNATQPTYY446 pKa = 10.18 TVNTLYY452 pKa = 10.26 PEE454 pKa = 4.29 LGGYY458 pKa = 9.32 VIEE461 pKa = 4.66 INADD465 pKa = 3.44 GTHH468 pKa = 6.22 GLVAAMQDD476 pKa = 3.13 QGTSNWYY483 pKa = 8.69 QANDD487 pKa = 3.93 LLSNAANHH495 pKa = 6.57 DD496 pKa = 3.81 VNGAKK501 pKa = 10.36 FKK503 pKa = 10.62 DD504 pKa = 3.15 WRR506 pKa = 11.84 IPTKK510 pKa = 10.63 RR511 pKa = 11.84 EE512 pKa = 3.36 LNLIYY517 pKa = 9.88 FQRR520 pKa = 11.84 ASIGAASYY528 pKa = 9.25 YY529 pKa = 9.58 WSSTEE534 pKa = 3.56 IDD536 pKa = 4.89 NYY538 pKa = 9.06 SAWYY542 pKa = 10.23 QFFPNGNQGYY552 pKa = 7.77 SFKK555 pKa = 11.14 SSTEE559 pKa = 3.5 YY560 pKa = 10.6 VRR562 pKa = 11.84 AVRR565 pKa = 11.84 AFF567 pKa = 3.27
Molecular weight: 54.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.516
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.668
EMBOSS 3.808
Sillero 3.961
Patrickios 1.24
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A2K8X8G8|A0A2K8X8G8_9FLAO Por_Secre_tail domain-containing protein OS=Nonlabens sp. MB-3u-79 OX=2058134 GN=CW736_07495 PE=4 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.63 LKK7 pKa = 10.48 PVTPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGYY21 pKa = 9.53 DD22 pKa = 4.32 AITTDD27 pKa = 3.72 KK28 pKa = 11.01 PEE30 pKa = 4.36 KK31 pKa = 10.55 SLLAPIKK38 pKa = 10.49 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NDD45 pKa = 3.41 SGRR48 pKa = 11.84 MTMRR52 pKa = 11.84 YY53 pKa = 9.61 KK54 pKa = 10.94 GGGHH58 pKa = 5.71 KK59 pKa = 9.74 RR60 pKa = 11.84 RR61 pKa = 11.84 YY62 pKa = 9.75 RR63 pKa = 11.84 IIDD66 pKa = 3.8 FKK68 pKa = 10.97 RR69 pKa = 11.84 NHH71 pKa = 6.8 HH72 pKa = 5.87 GVPATVASIEE82 pKa = 4.02 YY83 pKa = 10.1 DD84 pKa = 3.45 PNRR87 pKa = 11.84 TAFIALVNYY96 pKa = 9.88 QDD98 pKa = 3.41 GEE100 pKa = 3.91 KK101 pKa = 10.21 RR102 pKa = 11.84 YY103 pKa = 9.73 IVAPNGLKK111 pKa = 10.19 VGQNIVSGEE120 pKa = 4.1 TATPEE125 pKa = 3.8 VGNAMKK131 pKa = 10.7 LSQIPLGSIVSCIEE145 pKa = 3.64 LRR147 pKa = 11.84 PGQGATIARR156 pKa = 11.84 SAGSFAQLMARR167 pKa = 11.84 DD168 pKa = 3.96 GKK170 pKa = 10.33 YY171 pKa = 9.08 ATVKK175 pKa = 9.73 MPSGEE180 pKa = 4.05 TRR182 pKa = 11.84 LILQEE187 pKa = 4.06 CLATIGAVSNSDD199 pKa = 3.15 HH200 pKa = 6.2 QLVVSGKK207 pKa = 9.63 AGRR210 pKa = 11.84 SRR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 TRR221 pKa = 11.84 PVVMNPVDD229 pKa = 3.47 HH230 pKa = 7.05 PMGGGEE236 pKa = 4.28 GKK238 pKa = 10.51 SSGGHH243 pKa = 4.59 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.3 GYY255 pKa = 7.46 RR256 pKa = 11.84 TRR258 pKa = 11.84 DD259 pKa = 3.23 KK260 pKa = 11.38 NKK262 pKa = 10.31 ASTQYY267 pKa = 10.45 IIEE270 pKa = 4.13 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.07 KK274 pKa = 9.82
Molecular weight: 29.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.911
IPC_protein 10.774
Toseland 10.935
ProMoST 10.701
Dawson 11.008
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.242
Grimsley 11.052
Solomon 11.169
Lehninger 11.125
Nozaki 10.906
DTASelect 10.73
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.95
Patrickios 10.95
IPC_peptide 11.169
IPC2_peptide 9.677
IPC2.peptide.svr19 8.452
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2710
0
2710
909226
38
4979
335.5
37.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.916 ± 0.049
0.771 ± 0.026
5.925 ± 0.044
6.43 ± 0.055
4.992 ± 0.043
6.525 ± 0.05
1.861 ± 0.024
7.602 ± 0.045
6.924 ± 0.072
9.324 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.377 ± 0.026
5.617 ± 0.05
3.424 ± 0.028
3.709 ± 0.028
3.698 ± 0.034
6.578 ± 0.037
5.94 ± 0.058
6.325 ± 0.037
1.07 ± 0.016
3.991 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here