Vibrio phage VpaJT_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BK66|A0A411BK66_9CAUD Uncharacterized protein OS=Vibrio phage VpaJT_1 OX=2484247 GN=VpaJT1_76 PE=4 SV=1
MM1 pKa = 7.29ATDD4 pKa = 3.95PDD6 pKa = 4.56LPQLYY11 pKa = 10.59YY12 pKa = 10.98GGDD15 pKa = 3.54YY16 pKa = 10.73LRR18 pKa = 11.84PEE20 pKa = 4.23RR21 pKa = 11.84DD22 pKa = 3.08SYY24 pKa = 12.1SFTNPDD30 pKa = 2.84GARR33 pKa = 11.84RR34 pKa = 11.84TTTSGGPMRR43 pKa = 11.84IEE45 pKa = 4.07TDD47 pKa = 3.14HH48 pKa = 7.25LGGPFNVSVQYY59 pKa = 10.01YY60 pKa = 10.1CPTPQMARR68 pKa = 11.84WFQLFWLRR76 pKa = 11.84EE77 pKa = 4.08TFEE80 pKa = 4.15GSIPFQAALALEE92 pKa = 4.35SPEE95 pKa = 4.07VFEE98 pKa = 5.87DD99 pKa = 3.99YY100 pKa = 10.84VVRR103 pKa = 11.84LRR105 pKa = 11.84GAPQWNQFTGINGRR119 pKa = 11.84VSVSYY124 pKa = 10.28EE125 pKa = 3.82VEE127 pKa = 3.9QRR129 pKa = 11.84TADD132 pKa = 3.75YY133 pKa = 8.09EE134 pKa = 4.43TEE136 pKa = 3.91DD137 pKa = 3.62TQFWFYY143 pKa = 11.28QEE145 pKa = 4.6YY146 pKa = 10.9GDD148 pKa = 5.9DD149 pKa = 4.83ADD151 pKa = 5.31DD152 pKa = 4.16VLNEE156 pKa = 4.28LEE158 pKa = 4.54KK159 pKa = 10.56LTNPVMDD166 pKa = 3.12QWIPGG171 pKa = 3.55

Molecular weight: 19.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BJZ2|A0A411BJZ2_9CAUD Transcriptional activator adenine-specific DNA methyltransferase OS=Vibrio phage VpaJT_1 OX=2484247 GN=VpaJT1_21 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 5.62LKK4 pKa = 11.06QFLRR8 pKa = 11.84QLKK11 pKa = 8.69QQDD14 pKa = 3.89NITISFSHH22 pKa = 6.72LGYY25 pKa = 10.1HH26 pKa = 5.43VRR28 pKa = 11.84RR29 pKa = 11.84GTTMLDD35 pKa = 2.98TRR37 pKa = 11.84GSHH40 pKa = 6.71RR41 pKa = 11.84PVKK44 pKa = 10.28PNTRR48 pKa = 11.84EE49 pKa = 3.56EE50 pKa = 4.5RR51 pKa = 11.84IRR53 pKa = 11.84KK54 pKa = 9.18AGNLHH59 pKa = 6.51RR60 pKa = 11.84ALSSACRR67 pKa = 11.84YY68 pKa = 9.02MSDD71 pKa = 4.53DD72 pKa = 3.24MRR74 pKa = 11.84AMVGDD79 pKa = 4.94LLACMASEE87 pKa = 4.68GEE89 pKa = 4.19RR90 pKa = 11.84RR91 pKa = 3.5

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

17270

29

908

185.7

20.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.962 ± 0.273

1.459 ± 0.119

6.647 ± 0.183

6.543 ± 0.235

3.561 ± 0.17

7.064 ± 0.295

2.142 ± 0.163

5.2 ± 0.235

4.933 ± 0.233

7.852 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.124

4.719 ± 0.233

4.296 ± 0.192

3.868 ± 0.168

6.196 ± 0.304

5.756 ± 0.185

6.572 ± 0.298

7.597 ± 0.21

1.471 ± 0.099

3.59 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski