Amino acid dipepetide frequency for Vibrio phage VpaJT_1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.196AlaAla: 6.196 ± 0.795
1.1AlaCys: 1.1 ± 0.276
4.054AlaAsp: 4.054 ± 0.533
5.617AlaGlu: 5.617 ± 0.608
2.374AlaPhe: 2.374 ± 0.324
4.401AlaGly: 4.401 ± 0.569
1.448AlaHis: 1.448 ± 0.217
4.401AlaIle: 4.401 ± 0.522
4.227AlaLys: 4.227 ± 0.649
7.933AlaLeu: 7.933 ± 0.667
2.78AlaMet: 2.78 ± 0.457
3.011AlaAsn: 3.011 ± 0.377
3.417AlaPro: 3.417 ± 0.498
3.648AlaGln: 3.648 ± 0.489
5.849AlaArg: 5.849 ± 0.536
4.517AlaSer: 4.517 ± 0.597
4.864AlaThr: 4.864 ± 0.507
6.138AlaVal: 6.138 ± 0.586
0.984AlaTrp: 0.984 ± 0.231
2.664AlaTyr: 2.664 ± 0.321
0.0AlaXaa: 0.0 ± 0.0
Cys
1.042CysAla: 1.042 ± 0.299
0.232CysCys: 0.232 ± 0.143
0.869CysAsp: 0.869 ± 0.238
1.274CysGlu: 1.274 ± 0.294
0.232CysPhe: 0.232 ± 0.134
0.869CysGly: 0.869 ± 0.21
0.347CysHis: 0.347 ± 0.148
0.927CysIle: 0.927 ± 0.257
1.042CysLys: 1.042 ± 0.244
0.869CysLeu: 0.869 ± 0.258
0.579CysMet: 0.579 ± 0.15
0.695CysAsn: 0.695 ± 0.177
0.869CysPro: 0.869 ± 0.244
0.869CysGln: 0.869 ± 0.198
1.1CysArg: 1.1 ± 0.25
0.463CysSer: 0.463 ± 0.144
0.463CysThr: 0.463 ± 0.208
1.158CysVal: 1.158 ± 0.233
0.116CysTrp: 0.116 ± 0.077
0.579CysTyr: 0.579 ± 0.201
0.0CysXaa: 0.0 ± 0.0
Asp
6.601AspAla: 6.601 ± 0.649
0.927AspCys: 0.927 ± 0.201
3.996AspAsp: 3.996 ± 0.504
4.169AspGlu: 4.169 ± 0.461
2.258AspPhe: 2.258 ± 0.384
5.327AspGly: 5.327 ± 0.565
1.158AspHis: 1.158 ± 0.284
3.59AspIle: 3.59 ± 0.409
3.243AspLys: 3.243 ± 0.431
5.385AspLeu: 5.385 ± 0.517
1.853AspMet: 1.853 ± 0.299
2.953AspAsn: 2.953 ± 0.52
2.722AspPro: 2.722 ± 0.368
1.737AspGln: 1.737 ± 0.332
3.648AspArg: 3.648 ± 0.447
3.127AspSer: 3.127 ± 0.396
5.27AspThr: 5.27 ± 0.621
5.154AspVal: 5.154 ± 0.456
1.216AspTrp: 1.216 ± 0.314
2.143AspTyr: 2.143 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
5.675GluAla: 5.675 ± 0.667
0.753GluCys: 0.753 ± 0.209
3.069GluAsp: 3.069 ± 0.465
3.359GluGlu: 3.359 ± 0.43
2.664GluPhe: 2.664 ± 0.339
3.938GluGly: 3.938 ± 0.488
1.158GluHis: 1.158 ± 0.288
3.011GluIle: 3.011 ± 0.389
2.953GluLys: 2.953 ± 0.432
6.891GluLeu: 6.891 ± 0.978
1.795GluMet: 1.795 ± 0.366
3.301GluAsn: 3.301 ± 0.38
2.895GluPro: 2.895 ± 0.453
3.127GluGln: 3.127 ± 0.403
4.517GluArg: 4.517 ± 0.494
3.59GluSer: 3.59 ± 0.471
5.27GluThr: 5.27 ± 0.687
3.417GluVal: 3.417 ± 0.482
1.506GluTrp: 1.506 ± 0.286
2.548GluTyr: 2.548 ± 0.375
0.0GluXaa: 0.0 ± 0.0
Phe
2.085PheAla: 2.085 ± 0.354
0.405PheCys: 0.405 ± 0.136
2.78PheAsp: 2.78 ± 0.414
2.78PheGlu: 2.78 ± 0.302
1.679PhePhe: 1.679 ± 0.381
2.606PheGly: 2.606 ± 0.399
0.811PheHis: 0.811 ± 0.2
2.143PheIle: 2.143 ± 0.439
1.795PheLys: 1.795 ± 0.316
2.258PheLeu: 2.258 ± 0.33
0.869PheMet: 0.869 ± 0.228
2.374PheAsn: 2.374 ± 0.418
1.216PhePro: 1.216 ± 0.268
0.927PheGln: 0.927 ± 0.255
1.853PheArg: 1.853 ± 0.376
2.316PheSer: 2.316 ± 0.334
2.78PheThr: 2.78 ± 0.423
2.316PheVal: 2.316 ± 0.407
0.405PheTrp: 0.405 ± 0.174
1.216PheTyr: 1.216 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
5.096GlyAla: 5.096 ± 0.618
0.869GlyCys: 0.869 ± 0.279
4.98GlyAsp: 4.98 ± 0.559
4.806GlyGlu: 4.806 ± 0.435
3.011GlyPhe: 3.011 ± 0.402
4.98GlyGly: 4.98 ± 0.683
0.869GlyHis: 0.869 ± 0.217
3.938GlyIle: 3.938 ± 0.491
4.401GlyLys: 4.401 ± 0.5
5.617GlyLeu: 5.617 ± 0.558
1.911GlyMet: 1.911 ± 0.288
2.953GlyAsn: 2.953 ± 0.479
1.737GlyPro: 1.737 ± 0.303
2.664GlyGln: 2.664 ± 0.448
3.532GlyArg: 3.532 ± 0.459
4.98GlySer: 4.98 ± 0.483
4.401GlyThr: 4.401 ± 0.53
6.486GlyVal: 6.486 ± 0.546
1.042GlyTrp: 1.042 ± 0.281
2.374GlyTyr: 2.374 ± 0.333
0.0GlyXaa: 0.0 ± 0.0
His
1.621HisAla: 1.621 ± 0.26
0.579HisCys: 0.579 ± 0.22
1.563HisAsp: 1.563 ± 0.316
1.042HisGlu: 1.042 ± 0.242
0.521HisPhe: 0.521 ± 0.189
1.795HisGly: 1.795 ± 0.418
0.521HisHis: 0.521 ± 0.159
1.332HisIle: 1.332 ± 0.273
0.811HisLys: 0.811 ± 0.213
1.679HisLeu: 1.679 ± 0.304
0.695HisMet: 0.695 ± 0.185
1.216HisAsn: 1.216 ± 0.308
0.869HisPro: 0.869 ± 0.233
0.463HisGln: 0.463 ± 0.173
1.563HisArg: 1.563 ± 0.325
0.811HisSer: 0.811 ± 0.206
0.984HisThr: 0.984 ± 0.274
2.2HisVal: 2.2 ± 0.344
0.405HisTrp: 0.405 ± 0.151
0.753HisTyr: 0.753 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
4.227IleAla: 4.227 ± 0.507
0.695IleCys: 0.695 ± 0.281
4.459IleAsp: 4.459 ± 0.484
4.227IleGlu: 4.227 ± 0.402
1.506IlePhe: 1.506 ± 0.377
3.59IleGly: 3.59 ± 0.415
0.927IleHis: 0.927 ± 0.221
1.737IleIle: 1.737 ± 0.338
3.011IleLys: 3.011 ± 0.405
3.301IleLeu: 3.301 ± 0.419
1.216IleMet: 1.216 ± 0.248
3.127IleAsn: 3.127 ± 0.457
2.374IlePro: 2.374 ± 0.389
1.274IleGln: 1.274 ± 0.268
3.359IleArg: 3.359 ± 0.479
3.301IleSer: 3.301 ± 0.481
3.938IleThr: 3.938 ± 0.477
3.532IleVal: 3.532 ± 0.426
0.579IleTrp: 0.579 ± 0.175
1.621IleTyr: 1.621 ± 0.272
0.0IleXaa: 0.0 ± 0.0
Lys
4.575LysAla: 4.575 ± 0.621
0.579LysCys: 0.579 ± 0.184
2.374LysAsp: 2.374 ± 0.376
3.011LysGlu: 3.011 ± 0.564
1.969LysPhe: 1.969 ± 0.325
3.185LysGly: 3.185 ± 0.454
1.216LysHis: 1.216 ± 0.285
3.069LysIle: 3.069 ± 0.437
1.911LysLys: 1.911 ± 0.502
3.822LysLeu: 3.822 ± 0.487
1.332LysMet: 1.332 ± 0.281
1.911LysAsn: 1.911 ± 0.392
2.664LysPro: 2.664 ± 0.45
2.432LysGln: 2.432 ± 0.342
3.88LysArg: 3.88 ± 0.522
2.722LysSer: 2.722 ± 0.452
2.953LysThr: 2.953 ± 0.579
2.895LysVal: 2.895 ± 0.493
0.753LysTrp: 0.753 ± 0.252
2.085LysTyr: 2.085 ± 0.361
0.0LysXaa: 0.0 ± 0.0
Leu
6.717LeuAla: 6.717 ± 0.55
1.216LeuCys: 1.216 ± 0.242
5.038LeuAsp: 5.038 ± 0.594
5.559LeuGlu: 5.559 ± 0.551
2.606LeuPhe: 2.606 ± 0.32
5.849LeuGly: 5.849 ± 0.489
1.795LeuHis: 1.795 ± 0.335
4.459LeuIle: 4.459 ± 0.52
3.417LeuLys: 3.417 ± 0.446
5.733LeuLeu: 5.733 ± 0.655
1.969LeuMet: 1.969 ± 0.307
4.343LeuAsn: 4.343 ± 0.515
3.474LeuPro: 3.474 ± 0.356
3.069LeuGln: 3.069 ± 0.401
4.922LeuArg: 4.922 ± 0.641
4.169LeuSer: 4.169 ± 0.462
5.385LeuThr: 5.385 ± 0.588
5.154LeuVal: 5.154 ± 0.555
0.811LeuTrp: 0.811 ± 0.212
2.78LeuTyr: 2.78 ± 0.362
0.0LeuXaa: 0.0 ± 0.0
Met
2.143MetAla: 2.143 ± 0.36
0.405MetCys: 0.405 ± 0.156
1.563MetAsp: 1.563 ± 0.319
0.927MetGlu: 0.927 ± 0.188
1.042MetPhe: 1.042 ± 0.226
0.869MetGly: 0.869 ± 0.187
0.347MetHis: 0.347 ± 0.15
1.679MetIle: 1.679 ± 0.333
1.853MetLys: 1.853 ± 0.333
2.548MetLeu: 2.548 ± 0.417
0.811MetMet: 0.811 ± 0.205
1.1MetAsn: 1.1 ± 0.197
0.695MetPro: 0.695 ± 0.248
0.811MetGln: 0.811 ± 0.212
1.506MetArg: 1.506 ± 0.314
2.664MetSer: 2.664 ± 0.441
1.969MetThr: 1.969 ± 0.252
1.621MetVal: 1.621 ± 0.313
0.232MetTrp: 0.232 ± 0.116
0.927MetTyr: 0.927 ± 0.177
0.0MetXaa: 0.0 ± 0.0
Asn
3.822AsnAla: 3.822 ± 0.441
0.927AsnCys: 0.927 ± 0.237
2.953AsnAsp: 2.953 ± 0.341
2.548AsnGlu: 2.548 ± 0.382
1.274AsnPhe: 1.274 ± 0.295
4.633AsnGly: 4.633 ± 0.54
1.274AsnHis: 1.274 ± 0.281
2.722AsnIle: 2.722 ± 0.397
1.969AsnLys: 1.969 ± 0.329
3.127AsnLeu: 3.127 ± 0.423
0.927AsnMet: 0.927 ± 0.202
2.606AsnAsn: 2.606 ± 0.45
2.895AsnPro: 2.895 ± 0.442
2.143AsnGln: 2.143 ± 0.347
2.49AsnArg: 2.49 ± 0.43
2.316AsnSer: 2.316 ± 0.362
2.722AsnThr: 2.722 ± 0.444
3.822AsnVal: 3.822 ± 0.447
0.579AsnTrp: 0.579 ± 0.182
1.448AsnTyr: 1.448 ± 0.288
0.0AsnXaa: 0.0 ± 0.0
Pro
2.78ProAla: 2.78 ± 0.417
0.695ProCys: 0.695 ± 0.222
2.664ProAsp: 2.664 ± 0.443
3.648ProGlu: 3.648 ± 0.605
1.679ProPhe: 1.679 ± 0.275
2.722ProGly: 2.722 ± 0.355
0.984ProHis: 0.984 ± 0.182
2.085ProIle: 2.085 ± 0.279
1.853ProLys: 1.853 ± 0.318
2.49ProLeu: 2.49 ± 0.412
0.869ProMet: 0.869 ± 0.218
2.49ProAsn: 2.49 ± 0.37
1.621ProPro: 1.621 ± 0.324
1.563ProGln: 1.563 ± 0.411
2.49ProArg: 2.49 ± 0.425
2.722ProSer: 2.722 ± 0.391
3.822ProThr: 3.822 ± 0.43
3.359ProVal: 3.359 ± 0.506
0.811ProTrp: 0.811 ± 0.266
1.621ProTyr: 1.621 ± 0.343
0.0ProXaa: 0.0 ± 0.0
Gln
2.722GlnAla: 2.722 ± 0.389
0.405GlnCys: 0.405 ± 0.125
1.853GlnAsp: 1.853 ± 0.312
2.49GlnGlu: 2.49 ± 0.39
1.795GlnPhe: 1.795 ± 0.263
2.606GlnGly: 2.606 ± 0.44
1.158GlnHis: 1.158 ± 0.282
2.027GlnIle: 2.027 ± 0.299
1.563GlnLys: 1.563 ± 0.288
4.227GlnLeu: 4.227 ± 0.461
1.1GlnMet: 1.1 ± 0.218
1.448GlnAsn: 1.448 ± 0.275
1.911GlnPro: 1.911 ± 0.391
1.39GlnGln: 1.39 ± 0.276
2.953GlnArg: 2.953 ± 0.442
2.085GlnSer: 2.085 ± 0.422
1.853GlnThr: 1.853 ± 0.378
2.78GlnVal: 2.78 ± 0.353
0.869GlnTrp: 0.869 ± 0.222
1.448GlnTyr: 1.448 ± 0.234
0.0GlnXaa: 0.0 ± 0.0
Arg
5.212ArgAla: 5.212 ± 0.58
1.1ArgCys: 1.1 ± 0.255
5.038ArgAsp: 5.038 ± 0.555
3.532ArgGlu: 3.532 ± 0.509
2.258ArgPhe: 2.258 ± 0.348
4.401ArgGly: 4.401 ± 0.635
2.027ArgHis: 2.027 ± 0.358
3.011ArgIle: 3.011 ± 0.407
3.532ArgLys: 3.532 ± 0.454
4.285ArgLeu: 4.285 ± 0.605
1.39ArgMet: 1.39 ± 0.258
2.548ArgAsn: 2.548 ± 0.38
2.258ArgPro: 2.258 ± 0.372
2.722ArgGln: 2.722 ± 0.457
3.938ArgArg: 3.938 ± 0.657
2.837ArgSer: 2.837 ± 0.338
3.764ArgThr: 3.764 ± 0.466
4.748ArgVal: 4.748 ± 0.467
0.811ArgTrp: 0.811 ± 0.24
2.548ArgTyr: 2.548 ± 0.376
0.0ArgXaa: 0.0 ± 0.0
Ser
4.343SerAla: 4.343 ± 0.462
0.695SerCys: 0.695 ± 0.186
4.748SerAsp: 4.748 ± 0.51
3.996SerGlu: 3.996 ± 0.507
2.374SerPhe: 2.374 ± 0.392
5.559SerGly: 5.559 ± 0.625
1.39SerHis: 1.39 ± 0.363
2.606SerIle: 2.606 ± 0.317
2.78SerLys: 2.78 ± 0.451
3.822SerLeu: 3.822 ± 0.433
1.216SerMet: 1.216 ± 0.305
2.664SerAsn: 2.664 ± 0.367
2.78SerPro: 2.78 ± 0.366
1.969SerGln: 1.969 ± 0.276
2.953SerArg: 2.953 ± 0.372
2.432SerSer: 2.432 ± 0.392
3.243SerThr: 3.243 ± 0.518
5.617SerVal: 5.617 ± 0.582
0.405SerTrp: 0.405 ± 0.178
1.969SerTyr: 1.969 ± 0.368
0.0SerXaa: 0.0 ± 0.0
Thr
4.169ThrAla: 4.169 ± 0.515
0.927ThrCys: 0.927 ± 0.238
4.69ThrAsp: 4.69 ± 0.55
4.806ThrGlu: 4.806 ± 0.553
2.78ThrPhe: 2.78 ± 0.34
4.575ThrGly: 4.575 ± 0.571
1.1ThrHis: 1.1 ± 0.25
3.706ThrIle: 3.706 ± 0.518
2.606ThrLys: 2.606 ± 0.322
6.37ThrLeu: 6.37 ± 0.685
1.621ThrMet: 1.621 ± 0.288
2.548ThrAsn: 2.548 ± 0.391
3.648ThrPro: 3.648 ± 0.418
2.606ThrGln: 2.606 ± 0.557
2.895ThrArg: 2.895 ± 0.412
3.706ThrSer: 3.706 ± 0.412
3.474ThrThr: 3.474 ± 0.608
5.849ThrVal: 5.849 ± 0.694
1.042ThrTrp: 1.042 ± 0.2
2.606ThrTyr: 2.606 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
6.833ValAla: 6.833 ± 0.747
1.332ValCys: 1.332 ± 0.296
5.501ValAsp: 5.501 ± 0.405
4.98ValGlu: 4.98 ± 0.545
1.911ValPhe: 1.911 ± 0.363
4.864ValGly: 4.864 ± 0.569
1.216ValHis: 1.216 ± 0.261
3.359ValIle: 3.359 ± 0.556
3.822ValLys: 3.822 ± 0.51
5.212ValLeu: 5.212 ± 0.653
1.621ValMet: 1.621 ± 0.32
3.532ValAsn: 3.532 ± 0.544
3.301ValPro: 3.301 ± 0.501
3.59ValGln: 3.59 ± 0.45
5.096ValArg: 5.096 ± 0.503
5.154ValSer: 5.154 ± 0.487
5.385ValThr: 5.385 ± 0.702
5.791ValVal: 5.791 ± 0.568
1.332ValTrp: 1.332 ± 0.287
2.143ValTyr: 2.143 ± 0.372
0.0ValXaa: 0.0 ± 0.0
Trp
1.216TrpAla: 1.216 ± 0.323
0.637TrpCys: 0.637 ± 0.172
1.1TrpAsp: 1.1 ± 0.28
0.753TrpGlu: 0.753 ± 0.215
0.811TrpPhe: 0.811 ± 0.196
0.811TrpGly: 0.811 ± 0.308
0.463TrpHis: 0.463 ± 0.138
0.347TrpIle: 0.347 ± 0.157
0.695TrpLys: 0.695 ± 0.256
0.927TrpLeu: 0.927 ± 0.267
0.29TrpMet: 0.29 ± 0.118
0.463TrpAsn: 0.463 ± 0.147
0.405TrpPro: 0.405 ± 0.178
0.579TrpGln: 0.579 ± 0.215
0.927TrpArg: 0.927 ± 0.245
0.927TrpSer: 0.927 ± 0.206
0.811TrpThr: 0.811 ± 0.198
1.274TrpVal: 1.274 ± 0.271
0.174TrpTrp: 0.174 ± 0.093
1.1TrpTyr: 1.1 ± 0.276
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.548TyrAla: 2.548 ± 0.469
0.116TyrCys: 0.116 ± 0.096
3.185TyrAsp: 3.185 ± 0.499
1.911TyrGlu: 1.911 ± 0.342
0.869TyrPhe: 0.869 ± 0.221
3.069TyrGly: 3.069 ± 0.473
1.216TyrHis: 1.216 ± 0.23
1.853TyrIle: 1.853 ± 0.236
1.853TyrLys: 1.853 ± 0.372
2.027TyrLeu: 2.027 ± 0.336
0.811TyrMet: 0.811 ± 0.2
1.911TyrAsn: 1.911 ± 0.283
1.216TyrPro: 1.216 ± 0.298
1.1TyrGln: 1.1 ± 0.25
2.49TyrArg: 2.49 ± 0.411
2.722TyrSer: 2.722 ± 0.444
2.374TyrThr: 2.374 ± 0.425
2.664TyrVal: 2.664 ± 0.304
0.637TyrTrp: 0.637 ± 0.168
1.332TyrTyr: 1.332 ± 0.276
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (17270 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski