Streptococcus satellite phage Javan388

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZLY1|A0A4D5ZLY1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan388 OX=2558672 GN=JavanS388_0021 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.4NLEE5 pKa = 4.17EE6 pKa = 5.44KK7 pKa = 10.32IEE9 pKa = 3.99PVVIIIDD16 pKa = 3.8EE17 pKa = 4.17EE18 pKa = 4.64DD19 pKa = 3.74DD20 pKa = 3.93FDD22 pKa = 5.94FEE24 pKa = 4.36EE25 pKa = 4.73TDD27 pKa = 5.58DD28 pKa = 4.88EE29 pKa = 4.43IQAQYY34 pKa = 10.54EE35 pKa = 4.39AEE37 pKa = 4.18CLEE40 pKa = 5.78DD41 pKa = 3.71DD42 pKa = 4.11TQDD45 pKa = 3.43VINWINEE52 pKa = 4.21SNTLTEE58 pKa = 4.22YY59 pKa = 11.41VSMEE63 pKa = 4.41FEE65 pKa = 5.29NMNQDD70 pKa = 3.68DD71 pKa = 4.68LGASLEE77 pKa = 4.2NASQCLDD84 pKa = 4.31AIVGMLEE91 pKa = 3.76NGTLVLAGGKK101 pKa = 9.33HH102 pKa = 5.1EE103 pKa = 4.14NN104 pKa = 3.61

Molecular weight:
11.78 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZLM9|A0A4D5ZLM9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan388 OX=2558672 GN=JavanS388_0022 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.25KK3 pKa = 8.43ITEE6 pKa = 3.97IQDD9 pKa = 2.68IKK11 pKa = 9.74TFRR14 pKa = 11.84IIQAIVLVLAIYY26 pKa = 10.26ILIIKK31 pKa = 8.4WGNPFGVFFIIGISWLLSLLLPYY54 pKa = 9.99EE55 pKa = 3.97YY56 pKa = 10.33RR57 pKa = 11.84GGYY60 pKa = 9.67NKK62 pKa = 10.32AQKK65 pKa = 10.48NVFLKK70 pKa = 10.71NVSPLTEE77 pKa = 4.03NLISDD82 pKa = 4.17GLIALVVIILYY93 pKa = 9.09FLNKK97 pKa = 10.03

Molecular weight:
11.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3080

48

519

140.0

16.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.195 ± 0.631

0.714 ± 0.167

6.494 ± 0.578

8.669 ± 0.813

4.513 ± 0.471

4.253 ± 0.493

1.071 ± 0.167

9.123 ± 0.692

9.61 ± 0.77

10.227 ± 0.728

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.251

5.877 ± 0.491

2.078 ± 0.215

4.351 ± 0.362

3.831 ± 0.423

5.909 ± 0.538

5.162 ± 0.417

5.13 ± 0.472

0.844 ± 0.15

4.383 ± 0.213

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski