Streptococcus satellite phage Javan388
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLY1|A0A4D5ZLY1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan388 OX=2558672 GN=JavanS388_0021 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.4 NLEE5 pKa = 4.17 EE6 pKa = 5.44 KK7 pKa = 10.32 IEE9 pKa = 3.99 PVVIIIDD16 pKa = 3.8 EE17 pKa = 4.17 EE18 pKa = 4.64 DD19 pKa = 3.74 DD20 pKa = 3.93 FDD22 pKa = 5.94 FEE24 pKa = 4.36 EE25 pKa = 4.73 TDD27 pKa = 5.58 DD28 pKa = 4.88 EE29 pKa = 4.43 IQAQYY34 pKa = 10.54 EE35 pKa = 4.39 AEE37 pKa = 4.18 CLEE40 pKa = 5.78 DD41 pKa = 3.71 DD42 pKa = 4.11 TQDD45 pKa = 3.43 VINWINEE52 pKa = 4.21 SNTLTEE58 pKa = 4.22 YY59 pKa = 11.41 VSMEE63 pKa = 4.41 FEE65 pKa = 5.29 NMNQDD70 pKa = 3.68 DD71 pKa = 4.68 LGASLEE77 pKa = 4.2 NASQCLDD84 pKa = 4.31 AIVGMLEE91 pKa = 3.76 NGTLVLAGGKK101 pKa = 9.33 HH102 pKa = 5.1 EE103 pKa = 4.14 NN104 pKa = 3.61
Molecular weight: 11.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.011
IPC2_protein 3.401
IPC_protein 3.35
Toseland 3.172
ProMoST 3.528
Dawson 3.35
Bjellqvist 3.503
Wikipedia 3.261
Rodwell 3.198
Grimsley 3.083
Solomon 3.312
Lehninger 3.261
Nozaki 3.478
DTASelect 3.617
Thurlkill 3.223
EMBOSS 3.274
Sillero 3.478
Patrickios 1.697
IPC_peptide 3.312
IPC2_peptide 3.452
IPC2.peptide.svr19 3.647
Protein with the highest isoelectric point:
>tr|A0A4D5ZLM9|A0A4D5ZLM9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan388 OX=2558672 GN=JavanS388_0022 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.25 KK3 pKa = 8.43 ITEE6 pKa = 3.97 IQDD9 pKa = 2.68 IKK11 pKa = 9.74 TFRR14 pKa = 11.84 IIQAIVLVLAIYY26 pKa = 10.26 ILIIKK31 pKa = 8.4 WGNPFGVFFIIGISWLLSLLLPYY54 pKa = 9.99 EE55 pKa = 3.97 YY56 pKa = 10.33 RR57 pKa = 11.84 GGYY60 pKa = 9.67 NKK62 pKa = 10.32 AQKK65 pKa = 10.48 NVFLKK70 pKa = 10.71 NVSPLTEE77 pKa = 4.03 NLISDD82 pKa = 4.17 GLIALVVIILYY93 pKa = 9.09 FLNKK97 pKa = 10.03
Molecular weight: 11.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.112
IPC2_protein 9.209
IPC_protein 9.136
Toseland 9.721
ProMoST 9.575
Dawson 9.984
Bjellqvist 9.648
Wikipedia 10.16
Rodwell 10.365
Grimsley 10.058
Solomon 10.028
Lehninger 9.984
Nozaki 9.677
DTASelect 9.648
Thurlkill 9.809
EMBOSS 10.131
Sillero 9.882
Patrickios 7.571
IPC_peptide 10.014
IPC2_peptide 8.156
IPC2.peptide.svr19 8.209
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22
0
22
3080
48
519
140.0
16.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.195 ± 0.631
0.714 ± 0.167
6.494 ± 0.578
8.669 ± 0.813
4.513 ± 0.471
4.253 ± 0.493
1.071 ± 0.167
9.123 ± 0.692
9.61 ± 0.77
10.227 ± 0.728
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.565 ± 0.251
5.877 ± 0.491
2.078 ± 0.215
4.351 ± 0.362
3.831 ± 0.423
5.909 ± 0.538
5.162 ± 0.417
5.13 ± 0.472
0.844 ± 0.15
4.383 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here