Mycobacterium phage ShiLan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; Mycobacterium virus Shilan

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1DUP1|G1DUP1_9CAUD Transcriptional regulator OS=Mycobacterium phage ShiLan OX=1036616 GN=47 PE=4 SV=1
MM1 pKa = 7.24MSADD5 pKa = 3.89PVRR8 pKa = 11.84GAIQASLDD16 pKa = 3.5AMGDD20 pKa = 3.44GWQVAHH26 pKa = 5.73YY27 pKa = 9.08VVVVGLEE34 pKa = 4.17RR35 pKa = 11.84IDD37 pKa = 4.1GDD39 pKa = 4.67RR40 pKa = 11.84MDD42 pKa = 5.5LGATTVITPIGQAGYY57 pKa = 8.35VTDD60 pKa = 4.2GLVNRR65 pKa = 11.84YY66 pKa = 7.35WDD68 pKa = 3.73EE69 pKa = 4.28SSGEE73 pKa = 3.97

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1DUN7|G1DUN7_9CAUD Uncharacterized protein OS=Mycobacterium phage ShiLan OX=1036616 GN=43 PE=4 SV=1
MM1 pKa = 6.79THH3 pKa = 6.63SPGNRR8 pKa = 11.84QDD10 pKa = 4.13APSFTAGRR18 pKa = 11.84NGVRR22 pKa = 11.84HH23 pKa = 5.46RR24 pKa = 11.84RR25 pKa = 11.84IAFTHH30 pKa = 6.33FNLLSKK36 pKa = 10.86LDD38 pKa = 4.06LVRR41 pKa = 11.84TAYY44 pKa = 9.56KK45 pKa = 10.3VRR47 pKa = 11.84AYY49 pKa = 9.99PDD51 pKa = 3.54AEE53 pKa = 3.94QAALLRR59 pKa = 11.84RR60 pKa = 11.84TFGCVRR66 pKa = 11.84LVWNKK71 pKa = 8.63TLAEE75 pKa = 3.94RR76 pKa = 11.84QQRR79 pKa = 11.84YY80 pKa = 5.17TTEE83 pKa = 4.09QKK85 pKa = 8.79STSYY89 pKa = 11.18KK90 pKa = 8.93EE91 pKa = 3.49TDD93 pKa = 3.15AALSEE98 pKa = 4.27WKK100 pKa = 8.75KK101 pKa = 9.31TEE103 pKa = 3.98DD104 pKa = 3.43LAFLSEE110 pKa = 4.44VSSVPLQQTLRR121 pKa = 11.84HH122 pKa = 4.8QHH124 pKa = 5.1SAFAAFFKK132 pKa = 11.02GLAKK136 pKa = 10.48YY137 pKa = 9.91PRR139 pKa = 11.84FKK141 pKa = 10.64SRR143 pKa = 11.84HH144 pKa = 4.52GRR146 pKa = 11.84QSAHH150 pKa = 4.97FTRR153 pKa = 11.84SAFRR157 pKa = 11.84IKK159 pKa = 10.73DD160 pKa = 3.4GALWLAKK167 pKa = 9.15TATPLRR173 pKa = 11.84IVWTWPGVDD182 pKa = 4.93LAALDD187 pKa = 3.81PTMVIVSRR195 pKa = 11.84EE196 pKa = 3.56PDD198 pKa = 3.33GRR200 pKa = 11.84WFVTFAVDD208 pKa = 4.75QPDD211 pKa = 4.38PQPLPATGEE220 pKa = 4.38SVGVDD225 pKa = 3.21LGIKK229 pKa = 10.29DD230 pKa = 4.22FATLSTGEE238 pKa = 4.36KK239 pKa = 9.27IANPRR244 pKa = 11.84HH245 pKa = 5.1MARR248 pKa = 11.84HH249 pKa = 4.89EE250 pKa = 4.24RR251 pKa = 11.84GLRR254 pKa = 11.84RR255 pKa = 11.84QQRR258 pKa = 11.84RR259 pKa = 11.84LSRR262 pKa = 11.84MKK264 pKa = 10.38KK265 pKa = 8.77GSKK268 pKa = 9.34NRR270 pKa = 11.84ARR272 pKa = 11.84QRR274 pKa = 11.84VKK276 pKa = 10.29VARR279 pKa = 11.84KK280 pKa = 7.65HH281 pKa = 5.79ARR283 pKa = 11.84VRR285 pKa = 11.84DD286 pKa = 3.57ARR288 pKa = 11.84RR289 pKa = 11.84DD290 pKa = 3.87FLHH293 pKa = 6.02KK294 pKa = 9.64TSTEE298 pKa = 3.7LVRR301 pKa = 11.84RR302 pKa = 11.84FDD304 pKa = 4.03TIAVEE309 pKa = 5.02DD310 pKa = 4.2LAPKK314 pKa = 10.67NMVGNRR320 pKa = 11.84SLAKK324 pKa = 10.31SISEE328 pKa = 4.31CGWGEE333 pKa = 3.82FRR335 pKa = 11.84SMLEE339 pKa = 3.95YY340 pKa = 10.21KK341 pKa = 10.32AKK343 pKa = 9.93KK344 pKa = 8.98AGRR347 pKa = 11.84RR348 pKa = 11.84VAVINRR354 pKa = 11.84WYY356 pKa = 9.65PSSKK360 pKa = 8.31TCSACGHH367 pKa = 6.91LLATLSLGTRR377 pKa = 11.84HH378 pKa = 5.33WTCPDD383 pKa = 3.12CGTRR387 pKa = 11.84HH388 pKa = 6.6DD389 pKa = 4.59RR390 pKa = 11.84DD391 pKa = 3.06INAAKK396 pKa = 10.32NILVAAGLAEE406 pKa = 4.11TQNACGGDD414 pKa = 3.84VRR416 pKa = 11.84PHH418 pKa = 6.14GASHH422 pKa = 6.35RR423 pKa = 11.84QSPVKK428 pKa = 10.34QEE430 pKa = 3.98PSQATARR437 pKa = 11.84IPVLQGGEE445 pKa = 3.87

Molecular weight: 50.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

107

0

107

19329

25

1175

180.6

19.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.373 ± 0.448

1.236 ± 0.163

6.539 ± 0.208

5.82 ± 0.285

2.861 ± 0.169

8.868 ± 0.505

2.219 ± 0.179

4.32 ± 0.243

3.497 ± 0.203

7.181 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.119

3.332 ± 0.172

6.193 ± 0.208

3.482 ± 0.19

7.077 ± 0.359

5.934 ± 0.285

6.803 ± 0.259

7.248 ± 0.277

2.338 ± 0.143

2.411 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski