Amino acid dipepetide frequency for Mycobacterium phage ShiLan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.4AlaAla: 13.4 ± 1.777
1.087AlaCys: 1.087 ± 0.292
7.347AlaAsp: 7.347 ± 0.492
6.623AlaGlu: 6.623 ± 0.561
3.104AlaPhe: 3.104 ± 0.433
10.451AlaGly: 10.451 ± 1.228
2.225AlaHis: 2.225 ± 0.373
4.294AlaIle: 4.294 ± 0.489
4.398AlaLys: 4.398 ± 0.402
7.657AlaLeu: 7.657 ± 0.721
2.276AlaMet: 2.276 ± 0.4
2.742AlaAsn: 2.742 ± 0.401
4.915AlaPro: 4.915 ± 0.585
3.311AlaGln: 3.311 ± 0.423
6.985AlaArg: 6.985 ± 0.748
5.588AlaSer: 5.588 ± 0.567
6.571AlaThr: 6.571 ± 0.5
6.623AlaVal: 6.623 ± 0.578
2.018AlaTrp: 2.018 ± 0.327
2.121AlaTyr: 2.121 ± 0.311
0.0AlaXaa: 0.0 ± 0.0
Cys
0.828CysAla: 0.828 ± 0.275
0.0CysCys: 0.0 ± 0.0
0.983CysAsp: 0.983 ± 0.282
0.828CysGlu: 0.828 ± 0.225
0.155CysPhe: 0.155 ± 0.087
1.914CysGly: 1.914 ± 0.4
0.31CysHis: 0.31 ± 0.115
0.259CysIle: 0.259 ± 0.14
0.466CysLys: 0.466 ± 0.155
1.035CysLeu: 1.035 ± 0.305
0.052CysMet: 0.052 ± 0.053
0.466CysAsn: 0.466 ± 0.161
1.138CysPro: 1.138 ± 0.322
0.362CysGln: 0.362 ± 0.162
0.673CysArg: 0.673 ± 0.206
0.88CysSer: 0.88 ± 0.215
0.569CysThr: 0.569 ± 0.209
0.776CysVal: 0.776 ± 0.173
0.466CysTrp: 0.466 ± 0.151
0.207CysTyr: 0.207 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
6.829AspAla: 6.829 ± 0.649
0.621AspCys: 0.621 ± 0.165
4.294AspAsp: 4.294 ± 0.548
3.466AspGlu: 3.466 ± 0.436
1.707AspPhe: 1.707 ± 0.281
6.416AspGly: 6.416 ± 0.653
0.88AspHis: 0.88 ± 0.232
2.328AspIle: 2.328 ± 0.362
1.914AspLys: 1.914 ± 0.283
5.846AspLeu: 5.846 ± 0.447
1.19AspMet: 1.19 ± 0.255
2.018AspAsn: 2.018 ± 0.451
4.915AspPro: 4.915 ± 0.501
2.69AspGln: 2.69 ± 0.354
4.656AspArg: 4.656 ± 0.552
3.673AspSer: 3.673 ± 0.524
4.139AspThr: 4.139 ± 0.541
4.553AspVal: 4.553 ± 0.485
1.397AspTrp: 1.397 ± 0.253
1.863AspTyr: 1.863 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
5.433GluAla: 5.433 ± 0.647
1.087GluCys: 1.087 ± 0.308
3.311GluAsp: 3.311 ± 0.347
2.949GluGlu: 2.949 ± 0.561
1.966GluPhe: 1.966 ± 0.301
3.57GluGly: 3.57 ± 0.48
1.914GluHis: 1.914 ± 0.431
2.07GluIle: 2.07 ± 0.358
2.07GluLys: 2.07 ± 0.313
5.329GluLeu: 5.329 ± 0.663
1.5GluMet: 1.5 ± 0.268
1.604GluAsn: 1.604 ± 0.289
3.001GluPro: 3.001 ± 0.372
2.742GluGln: 2.742 ± 0.326
5.122GluArg: 5.122 ± 0.592
2.742GluSer: 2.742 ± 0.41
4.191GluThr: 4.191 ± 0.537
4.243GluVal: 4.243 ± 0.517
1.5GluTrp: 1.5 ± 0.265
1.863GluTyr: 1.863 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
2.846PheAla: 2.846 ± 0.357
0.155PheCys: 0.155 ± 0.091
2.949PheAsp: 2.949 ± 0.428
1.604PheGlu: 1.604 ± 0.233
0.828PhePhe: 0.828 ± 0.19
2.587PheGly: 2.587 ± 0.618
0.517PheHis: 0.517 ± 0.166
1.397PheIle: 1.397 ± 0.291
0.828PheLys: 0.828 ± 0.171
1.863PheLeu: 1.863 ± 0.299
0.724PheMet: 0.724 ± 0.181
1.293PheAsn: 1.293 ± 0.323
1.552PhePro: 1.552 ± 0.285
1.087PheGln: 1.087 ± 0.303
1.759PheArg: 1.759 ± 0.291
1.138PheSer: 1.138 ± 0.328
2.07PheThr: 2.07 ± 0.333
2.018PheVal: 2.018 ± 0.255
0.414PheTrp: 0.414 ± 0.122
0.983PheTyr: 0.983 ± 0.245
0.0PheXaa: 0.0 ± 0.0
Gly
9.261GlyAla: 9.261 ± 1.128
1.242GlyCys: 1.242 ± 0.277
5.381GlyAsp: 5.381 ± 0.516
4.656GlyGlu: 4.656 ± 0.524
2.742GlyPhe: 2.742 ± 0.426
10.555GlyGly: 10.555 ± 2.29
1.914GlyHis: 1.914 ± 0.291
3.622GlyIle: 3.622 ± 0.552
2.69GlyLys: 2.69 ± 0.389
5.122GlyLeu: 5.122 ± 0.491
2.432GlyMet: 2.432 ± 0.512
3.415GlyAsn: 3.415 ± 0.445
4.294GlyPro: 4.294 ± 0.65
2.018GlyGln: 2.018 ± 0.528
5.846GlyArg: 5.846 ± 0.647
6.364GlySer: 6.364 ± 0.975
6.933GlyThr: 6.933 ± 0.906
5.743GlyVal: 5.743 ± 0.536
2.69GlyTrp: 2.69 ± 0.369
1.707GlyTyr: 1.707 ± 0.384
0.0GlyXaa: 0.0 ± 0.0
His
1.811HisAla: 1.811 ± 0.358
0.362HisCys: 0.362 ± 0.147
1.19HisAsp: 1.19 ± 0.233
1.087HisGlu: 1.087 ± 0.248
0.569HisPhe: 0.569 ± 0.172
1.552HisGly: 1.552 ± 0.296
0.673HisHis: 0.673 ± 0.221
1.449HisIle: 1.449 ± 0.299
1.087HisLys: 1.087 ± 0.224
1.449HisLeu: 1.449 ± 0.252
0.621HisMet: 0.621 ± 0.137
0.776HisAsn: 0.776 ± 0.17
1.397HisPro: 1.397 ± 0.347
0.828HisGln: 0.828 ± 0.219
2.38HisArg: 2.38 ± 0.435
0.88HisSer: 0.88 ± 0.186
1.552HisThr: 1.552 ± 0.372
1.449HisVal: 1.449 ± 0.325
0.414HisTrp: 0.414 ± 0.13
0.673HisTyr: 0.673 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
5.174IleAla: 5.174 ± 0.497
0.621IleCys: 0.621 ± 0.205
3.777IleAsp: 3.777 ± 0.47
3.156IleGlu: 3.156 ± 0.414
0.621IlePhe: 0.621 ± 0.199
4.243IleGly: 4.243 ± 0.457
1.345IleHis: 1.345 ± 0.269
1.449IleIle: 1.449 ± 0.287
1.087IleLys: 1.087 ± 0.259
2.121IleLeu: 2.121 ± 0.336
0.31IleMet: 0.31 ± 0.116
1.345IleAsn: 1.345 ± 0.282
3.104IlePro: 3.104 ± 0.366
1.397IleGln: 1.397 ± 0.231
2.794IleArg: 2.794 ± 0.447
2.173IleSer: 2.173 ± 0.441
3.829IleThr: 3.829 ± 0.534
3.053IleVal: 3.053 ± 0.35
0.931IleTrp: 0.931 ± 0.224
0.673IleTyr: 0.673 ± 0.167
0.0IleXaa: 0.0 ± 0.0
Lys
3.725LysAla: 3.725 ± 0.549
0.621LysCys: 0.621 ± 0.152
1.759LysAsp: 1.759 ± 0.28
1.293LysGlu: 1.293 ± 0.272
1.397LysPhe: 1.397 ± 0.232
2.69LysGly: 2.69 ± 0.385
1.035LysHis: 1.035 ± 0.322
0.931LysIle: 0.931 ± 0.221
1.656LysLys: 1.656 ± 0.371
2.742LysLeu: 2.742 ± 0.476
0.569LysMet: 0.569 ± 0.148
1.19LysAsn: 1.19 ± 0.284
2.846LysPro: 2.846 ± 0.46
1.656LysGln: 1.656 ± 0.281
2.38LysArg: 2.38 ± 0.396
2.276LysSer: 2.276 ± 0.315
1.914LysThr: 1.914 ± 0.394
2.69LysVal: 2.69 ± 0.374
0.828LysTrp: 0.828 ± 0.221
0.776LysTyr: 0.776 ± 0.184
0.0LysXaa: 0.0 ± 0.0
Leu
8.019LeuAla: 8.019 ± 0.768
0.724LeuCys: 0.724 ± 0.233
4.036LeuAsp: 4.036 ± 0.652
4.087LeuGlu: 4.087 ± 0.539
2.121LeuPhe: 2.121 ± 0.264
4.915LeuGly: 4.915 ± 0.495
1.035LeuHis: 1.035 ± 0.248
2.794LeuIle: 2.794 ± 0.363
2.432LeuLys: 2.432 ± 0.436
4.967LeuLeu: 4.967 ± 0.567
1.914LeuMet: 1.914 ± 0.297
2.587LeuAsn: 2.587 ± 0.364
5.277LeuPro: 5.277 ± 0.625
2.897LeuGln: 2.897 ± 0.416
5.174LeuArg: 5.174 ± 0.731
4.967LeuSer: 4.967 ± 0.493
5.588LeuThr: 5.588 ± 0.516
4.863LeuVal: 4.863 ± 0.544
1.345LeuTrp: 1.345 ± 0.274
2.07LeuTyr: 2.07 ± 0.376
0.0LeuXaa: 0.0 ± 0.0
Met
2.121MetAla: 2.121 ± 0.352
0.155MetCys: 0.155 ± 0.122
1.604MetAsp: 1.604 ± 0.299
1.035MetGlu: 1.035 ± 0.229
0.517MetPhe: 0.517 ± 0.163
1.604MetGly: 1.604 ± 0.237
0.155MetHis: 0.155 ± 0.094
0.724MetIle: 0.724 ± 0.232
0.621MetLys: 0.621 ± 0.197
2.07MetLeu: 2.07 ± 0.288
0.414MetMet: 0.414 ± 0.186
0.931MetAsn: 0.931 ± 0.226
1.242MetPro: 1.242 ± 0.252
0.517MetGln: 0.517 ± 0.164
1.914MetArg: 1.914 ± 0.3
2.587MetSer: 2.587 ± 0.35
2.121MetThr: 2.121 ± 0.388
1.5MetVal: 1.5 ± 0.326
0.362MetTrp: 0.362 ± 0.122
0.466MetTyr: 0.466 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
3.311AsnAla: 3.311 ± 0.374
0.414AsnCys: 0.414 ± 0.16
1.656AsnAsp: 1.656 ± 0.259
1.5AsnGlu: 1.5 ± 0.36
0.724AsnPhe: 0.724 ± 0.224
4.243AsnGly: 4.243 ± 0.528
1.035AsnHis: 1.035 ± 0.255
1.604AsnIle: 1.604 ± 0.437
1.138AsnLys: 1.138 ± 0.274
2.483AsnLeu: 2.483 ± 0.345
0.828AsnMet: 0.828 ± 0.188
1.656AsnAsn: 1.656 ± 0.362
2.794AsnPro: 2.794 ± 0.315
0.983AsnGln: 0.983 ± 0.329
1.966AsnArg: 1.966 ± 0.392
1.449AsnSer: 1.449 ± 0.292
1.966AsnThr: 1.966 ± 0.347
2.173AsnVal: 2.173 ± 0.296
0.776AsnTrp: 0.776 ± 0.199
0.621AsnTyr: 0.621 ± 0.159
0.0AsnXaa: 0.0 ± 0.0
Pro
5.122ProAla: 5.122 ± 0.609
0.673ProCys: 0.673 ± 0.161
4.191ProAsp: 4.191 ± 0.481
4.139ProGlu: 4.139 ± 0.524
1.759ProPhe: 1.759 ± 0.374
6.364ProGly: 6.364 ± 0.682
1.707ProHis: 1.707 ± 0.306
1.863ProIle: 1.863 ± 0.37
2.173ProLys: 2.173 ± 0.365
4.708ProLeu: 4.708 ± 0.527
1.449ProMet: 1.449 ± 0.297
2.432ProAsn: 2.432 ± 0.403
4.191ProPro: 4.191 ± 0.574
2.587ProGln: 2.587 ± 0.362
3.466ProArg: 3.466 ± 0.487
3.829ProSer: 3.829 ± 0.477
4.087ProThr: 4.087 ± 0.503
4.243ProVal: 4.243 ± 0.438
1.19ProTrp: 1.19 ± 0.253
1.759ProTyr: 1.759 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
4.243GlnAla: 4.243 ± 0.545
0.466GlnCys: 0.466 ± 0.18
1.552GlnAsp: 1.552 ± 0.234
1.707GlnGlu: 1.707 ± 0.27
1.138GlnPhe: 1.138 ± 0.277
2.535GlnGly: 2.535 ± 0.472
1.138GlnHis: 1.138 ± 0.259
2.121GlnIle: 2.121 ± 0.347
1.345GlnLys: 1.345 ± 0.243
2.587GlnLeu: 2.587 ± 0.366
0.673GlnMet: 0.673 ± 0.196
0.88GlnAsn: 0.88 ± 0.26
2.69GlnPro: 2.69 ± 0.408
1.293GlnGln: 1.293 ± 0.306
2.535GlnArg: 2.535 ± 0.37
2.225GlnSer: 2.225 ± 0.301
1.552GlnThr: 1.552 ± 0.255
2.587GlnVal: 2.587 ± 0.346
0.776GlnTrp: 0.776 ± 0.24
0.776GlnTyr: 0.776 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
6.881ArgAla: 6.881 ± 0.605
1.19ArgCys: 1.19 ± 0.3
4.346ArgAsp: 4.346 ± 0.478
5.226ArgGlu: 5.226 ± 0.647
1.966ArgPhe: 1.966 ± 0.383
3.984ArgGly: 3.984 ± 0.468
1.552ArgHis: 1.552 ± 0.47
4.294ArgIle: 4.294 ± 0.505
2.639ArgLys: 2.639 ± 0.373
4.915ArgLeu: 4.915 ± 0.562
2.483ArgMet: 2.483 ± 0.342
2.587ArgAsn: 2.587 ± 0.39
3.673ArgPro: 3.673 ± 0.445
1.966ArgGln: 1.966 ± 0.442
5.95ArgArg: 5.95 ± 0.734
3.777ArgSer: 3.777 ± 0.463
3.932ArgThr: 3.932 ± 0.481
5.277ArgVal: 5.277 ± 0.58
2.018ArgTrp: 2.018 ± 0.323
2.121ArgTyr: 2.121 ± 0.25
0.0ArgXaa: 0.0 ± 0.0
Ser
6.364SerAla: 6.364 ± 0.764
0.362SerCys: 0.362 ± 0.157
4.036SerAsp: 4.036 ± 0.539
3.208SerGlu: 3.208 ± 0.438
1.707SerPhe: 1.707 ± 0.288
6.312SerGly: 6.312 ± 0.971
0.88SerHis: 0.88 ± 0.174
2.794SerIle: 2.794 ± 0.425
2.276SerLys: 2.276 ± 0.402
3.518SerLeu: 3.518 ± 0.457
1.345SerMet: 1.345 ± 0.247
2.018SerAsn: 2.018 ± 0.397
3.466SerPro: 3.466 ± 0.413
1.759SerGln: 1.759 ± 0.273
3.622SerArg: 3.622 ± 0.315
4.191SerSer: 4.191 ± 0.72
4.087SerThr: 4.087 ± 0.537
4.967SerVal: 4.967 ± 0.618
1.138SerTrp: 1.138 ± 0.244
1.293SerTyr: 1.293 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
7.036ThrAla: 7.036 ± 0.583
0.828ThrCys: 0.828 ± 0.254
4.191ThrAsp: 4.191 ± 0.544
4.294ThrGlu: 4.294 ± 0.451
1.811ThrPhe: 1.811 ± 0.296
6.467ThrGly: 6.467 ± 0.672
1.707ThrHis: 1.707 ± 0.33
3.518ThrIle: 3.518 ± 0.511
1.914ThrLys: 1.914 ± 0.355
4.036ThrLeu: 4.036 ± 0.412
1.449ThrMet: 1.449 ± 0.265
1.759ThrAsn: 1.759 ± 0.332
4.76ThrPro: 4.76 ± 0.511
2.276ThrGln: 2.276 ± 0.314
4.45ThrArg: 4.45 ± 0.485
4.243ThrSer: 4.243 ± 0.517
4.708ThrThr: 4.708 ± 0.58
5.795ThrVal: 5.795 ± 0.521
1.293ThrTrp: 1.293 ± 0.28
1.5ThrTyr: 1.5 ± 0.287
0.0ThrXaa: 0.0 ± 0.0
Val
7.295ValAla: 7.295 ± 0.602
1.138ValCys: 1.138 ± 0.223
5.329ValAsp: 5.329 ± 0.472
4.294ValGlu: 4.294 ± 0.469
2.483ValPhe: 2.483 ± 0.335
5.174ValGly: 5.174 ± 0.569
1.242ValHis: 1.242 ± 0.278
3.26ValIle: 3.26 ± 0.396
2.535ValLys: 2.535 ± 0.394
5.588ValLeu: 5.588 ± 0.664
1.293ValMet: 1.293 ± 0.248
2.225ValAsn: 2.225 ± 0.349
3.673ValPro: 3.673 ± 0.364
2.535ValGln: 2.535 ± 0.329
4.915ValArg: 4.915 ± 0.664
4.346ValSer: 4.346 ± 0.556
5.07ValThr: 5.07 ± 0.462
6.26ValVal: 6.26 ± 0.739
2.38ValTrp: 2.38 ± 0.375
1.449ValTyr: 1.449 ± 0.274
0.0ValXaa: 0.0 ± 0.0
Trp
1.863TrpAla: 1.863 ± 0.271
0.362TrpCys: 0.362 ± 0.121
1.449TrpAsp: 1.449 ± 0.276
1.087TrpGlu: 1.087 ± 0.321
0.724TrpPhe: 0.724 ± 0.175
1.242TrpGly: 1.242 ± 0.245
0.569TrpHis: 0.569 ± 0.19
1.19TrpIle: 1.19 ± 0.236
0.931TrpLys: 0.931 ± 0.195
1.863TrpLeu: 1.863 ± 0.367
0.931TrpMet: 0.931 ± 0.228
0.673TrpAsn: 0.673 ± 0.198
1.19TrpPro: 1.19 ± 0.247
1.19TrpGln: 1.19 ± 0.276
2.328TrpArg: 2.328 ± 0.364
1.293TrpSer: 1.293 ± 0.284
1.552TrpThr: 1.552 ± 0.304
1.552TrpVal: 1.552 ± 0.381
0.828TrpTrp: 0.828 ± 0.194
0.569TrpTyr: 0.569 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.173TyrAla: 2.173 ± 0.363
0.259TyrCys: 0.259 ± 0.108
2.018TyrAsp: 2.018 ± 0.336
1.966TyrGlu: 1.966 ± 0.301
0.569TyrPhe: 0.569 ± 0.172
1.863TyrGly: 1.863 ± 0.348
0.362TyrHis: 0.362 ± 0.136
1.242TyrIle: 1.242 ± 0.206
0.776TyrLys: 0.776 ± 0.221
1.914TyrLeu: 1.914 ± 0.31
0.155TyrMet: 0.155 ± 0.094
0.724TyrAsn: 0.724 ± 0.17
1.811TyrPro: 1.811 ± 0.325
0.724TyrGln: 0.724 ± 0.182
1.863TyrArg: 1.863 ± 0.312
0.724TyrSer: 0.724 ± 0.178
1.604TyrThr: 1.604 ± 0.263
2.121TyrVal: 2.121 ± 0.335
0.621TyrTrp: 0.621 ± 0.184
0.621TyrTyr: 0.621 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (19329 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski