Gordonia phage TillyBobJoe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wizardvirus; unclassified Wizardvirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DTZ2|A0A385DTZ2_9CAUD RecT-like ssDNA binding protein OS=Gordonia phage TillyBobJoe OX=2301560 GN=57 PE=4 SV=1
MM1 pKa = 7.39IGLVKK6 pKa = 10.62SLTGSDD12 pKa = 3.15GSGFDD17 pKa = 3.75EE18 pKa = 4.73NEE20 pKa = 3.96PAPVVHH26 pKa = 6.95SVEE29 pKa = 4.05QDD31 pKa = 2.79GDD33 pKa = 3.72TGTTLIDD40 pKa = 3.11TTTYY44 pKa = 8.29HH45 pKa = 6.3WEE47 pKa = 4.02YY48 pKa = 9.35STSGGWRR55 pKa = 11.84FNCTGLMPTLEE66 pKa = 4.62VNTEE70 pKa = 3.72PAPTYY75 pKa = 10.02TEE77 pKa = 5.17EE78 pKa = 4.17PTQDD82 pKa = 3.56PVVLSGPCPAVGEE95 pKa = 4.2RR96 pKa = 11.84SEE98 pKa = 4.69DD99 pKa = 3.51ASGTPMRR106 pKa = 11.84CIAADD111 pKa = 4.23GYY113 pKa = 11.14GGQRR117 pKa = 11.84WAPAPP122 pKa = 3.71

Molecular weight: 12.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DUV4|A0A385DUV4_9CAUD Uncharacterized protein OS=Gordonia phage TillyBobJoe OX=2301560 GN=48 PE=4 SV=1
MM1 pKa = 7.66TPRR4 pKa = 11.84QVDD7 pKa = 3.43RR8 pKa = 11.84EE9 pKa = 4.3GNEE12 pKa = 3.21LRR14 pKa = 11.84TRR16 pKa = 11.84QRR18 pKa = 11.84VVYY21 pKa = 10.19VGMLGTMSEE30 pKa = 4.32PGRR33 pKa = 11.84VSRR36 pKa = 11.84VLKK39 pKa = 10.3ASVRR43 pKa = 11.84VRR45 pKa = 11.84FDD47 pKa = 3.18DD48 pKa = 4.0GRR50 pKa = 11.84VEE52 pKa = 4.32DD53 pKa = 3.88VHH55 pKa = 8.36PEE57 pKa = 3.85GLRR60 pKa = 11.84RR61 pKa = 11.84SAPIVNEE68 pKa = 3.86PNGNSSFVPKK78 pKa = 10.46PCDD81 pKa = 3.16FWDD84 pKa = 4.01RR85 pKa = 11.84STQTGRR91 pKa = 11.84KK92 pKa = 8.63

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

18773

29

1844

201.9

21.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.193 ± 0.483

0.922 ± 0.158

7.292 ± 0.326

5.678 ± 0.304

2.445 ± 0.156

8.72 ± 0.487

2.067 ± 0.232

4.192 ± 0.206

2.834 ± 0.225

8.038 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.123

2.77 ± 0.161

6.323 ± 0.304

3.345 ± 0.151

7.473 ± 0.428

5.108 ± 0.255

6.989 ± 0.334

7.489 ± 0.229

2.12 ± 0.143

1.971 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski