Amino acid dipepetide frequency for Gordonia phage TillyBobJoe

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.846AlaAla: 17.846 ± 1.869
1.172AlaCys: 1.172 ± 0.307
8.257AlaAsp: 8.257 ± 0.8
8.736AlaGlu: 8.736 ± 0.728
2.344AlaPhe: 2.344 ± 0.362
9.535AlaGly: 9.535 ± 1.036
2.024AlaHis: 2.024 ± 0.445
5.913AlaIle: 5.913 ± 0.586
4.049AlaLys: 4.049 ± 0.495
10.707AlaLeu: 10.707 ± 1.016
3.356AlaMet: 3.356 ± 0.297
3.516AlaAsn: 3.516 ± 0.501
6.765AlaPro: 6.765 ± 0.749
4.155AlaGln: 4.155 ± 0.615
8.31AlaArg: 8.31 ± 0.71
5.061AlaSer: 5.061 ± 0.612
7.192AlaThr: 7.192 ± 0.787
8.47AlaVal: 8.47 ± 0.799
2.184AlaTrp: 2.184 ± 0.357
2.344AlaTyr: 2.344 ± 0.32
0.0AlaXaa: 0.0 ± 0.0
Cys
1.119CysAla: 1.119 ± 0.332
0.16CysCys: 0.16 ± 0.098
1.172CysAsp: 1.172 ± 0.309
0.479CysGlu: 0.479 ± 0.166
0.16CysPhe: 0.16 ± 0.095
1.172CysGly: 1.172 ± 0.312
0.16CysHis: 0.16 ± 0.095
0.053CysIle: 0.053 ± 0.068
0.0CysLys: 0.0 ± 0.0
0.266CysLeu: 0.266 ± 0.14
0.107CysMet: 0.107 ± 0.086
0.266CysAsn: 0.266 ± 0.113
1.012CysPro: 1.012 ± 0.295
0.107CysGln: 0.107 ± 0.086
0.746CysArg: 0.746 ± 0.229
0.852CysSer: 0.852 ± 0.22
0.746CysThr: 0.746 ± 0.223
0.533CysVal: 0.533 ± 0.16
0.053CysTrp: 0.053 ± 0.05
0.053CysTyr: 0.053 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
8.044AspAla: 8.044 ± 0.66
0.852AspCys: 0.852 ± 0.257
6.126AspAsp: 6.126 ± 0.729
5.38AspGlu: 5.38 ± 0.64
1.811AspPhe: 1.811 ± 0.31
6.233AspGly: 6.233 ± 0.778
1.758AspHis: 1.758 ± 0.312
1.598AspIle: 1.598 ± 0.369
1.278AspLys: 1.278 ± 0.321
6.712AspLeu: 6.712 ± 0.643
1.065AspMet: 1.065 ± 0.224
2.291AspAsn: 2.291 ± 0.402
5.966AspPro: 5.966 ± 0.621
2.184AspGln: 2.184 ± 0.362
5.38AspArg: 5.38 ± 0.777
2.823AspSer: 2.823 ± 0.488
4.528AspThr: 4.528 ± 0.499
5.647AspVal: 5.647 ± 0.654
1.598AspTrp: 1.598 ± 0.293
1.651AspTyr: 1.651 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
6.606GluAla: 6.606 ± 0.566
0.639GluCys: 0.639 ± 0.194
3.409GluAsp: 3.409 ± 0.447
2.61GluGlu: 2.61 ± 0.487
1.545GluPhe: 1.545 ± 0.273
3.942GluGly: 3.942 ± 0.578
1.065GluHis: 1.065 ± 0.271
2.504GluIle: 2.504 ± 0.386
1.971GluLys: 1.971 ± 0.36
6.339GluLeu: 6.339 ± 0.769
0.959GluMet: 0.959 ± 0.216
1.492GluAsn: 1.492 ± 0.245
3.516GluPro: 3.516 ± 0.588
3.09GluGln: 3.09 ± 0.481
4.049GluArg: 4.049 ± 0.645
2.823GluSer: 2.823 ± 0.372
2.557GluThr: 2.557 ± 0.348
4.421GluVal: 4.421 ± 0.449
1.811GluTrp: 1.811 ± 0.348
1.438GluTyr: 1.438 ± 0.28
0.0GluXaa: 0.0 ± 0.0
Phe
2.823PheAla: 2.823 ± 0.376
0.107PheCys: 0.107 ± 0.066
2.291PheAsp: 2.291 ± 0.365
1.438PheGlu: 1.438 ± 0.296
1.065PhePhe: 1.065 ± 0.253
1.971PheGly: 1.971 ± 0.317
0.799PheHis: 0.799 ± 0.221
0.799PheIle: 0.799 ± 0.203
0.586PheLys: 0.586 ± 0.171
1.438PheLeu: 1.438 ± 0.292
0.32PheMet: 0.32 ± 0.16
1.012PheAsn: 1.012 ± 0.348
1.225PhePro: 1.225 ± 0.334
0.639PheGln: 0.639 ± 0.206
2.131PheArg: 2.131 ± 0.37
1.492PheSer: 1.492 ± 0.378
1.918PheThr: 1.918 ± 0.316
1.864PheVal: 1.864 ± 0.325
0.32PheTrp: 0.32 ± 0.113
0.213PheTyr: 0.213 ± 0.112
0.0PheXaa: 0.0 ± 0.0
Gly
8.257GlyAla: 8.257 ± 1.116
0.586GlyCys: 0.586 ± 0.201
6.286GlyAsp: 6.286 ± 0.752
4.208GlyGlu: 4.208 ± 0.504
2.504GlyPhe: 2.504 ± 0.376
9.482GlyGly: 9.482 ± 1.568
1.864GlyHis: 1.864 ± 0.428
4.208GlyIle: 4.208 ± 0.497
3.036GlyLys: 3.036 ± 0.526
6.606GlyLeu: 6.606 ± 0.53
2.078GlyMet: 2.078 ± 0.348
2.61GlyAsn: 2.61 ± 0.413
4.421GlyPro: 4.421 ± 0.53
3.356GlyGln: 3.356 ± 0.48
6.446GlyArg: 6.446 ± 0.699
4.528GlySer: 4.528 ± 0.679
5.38GlyThr: 5.38 ± 0.802
7.884GlyVal: 7.884 ± 0.664
1.438GlyTrp: 1.438 ± 0.289
2.024GlyTyr: 2.024 ± 0.333
0.0GlyXaa: 0.0 ± 0.0
His
1.918HisAla: 1.918 ± 0.335
0.213HisCys: 0.213 ± 0.106
1.278HisAsp: 1.278 ± 0.266
0.959HisGlu: 0.959 ± 0.216
0.479HisPhe: 0.479 ± 0.158
1.598HisGly: 1.598 ± 0.277
0.479HisHis: 0.479 ± 0.219
1.065HisIle: 1.065 ± 0.186
0.213HisLys: 0.213 ± 0.105
1.811HisLeu: 1.811 ± 0.282
0.32HisMet: 0.32 ± 0.138
0.586HisAsn: 0.586 ± 0.206
1.705HisPro: 1.705 ± 0.334
0.799HisGln: 0.799 ± 0.234
2.131HisArg: 2.131 ± 0.516
0.533HisSer: 0.533 ± 0.171
1.758HisThr: 1.758 ± 0.398
1.598HisVal: 1.598 ± 0.344
0.533HisTrp: 0.533 ± 0.16
0.693HisTyr: 0.693 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
5.913IleAla: 5.913 ± 0.612
0.213IleCys: 0.213 ± 0.122
3.569IleAsp: 3.569 ± 0.451
2.823IleGlu: 2.823 ± 0.471
0.852IlePhe: 0.852 ± 0.253
3.995IleGly: 3.995 ± 0.555
0.586IleHis: 0.586 ± 0.16
1.598IleIle: 1.598 ± 0.346
1.119IleLys: 1.119 ± 0.317
2.77IleLeu: 2.77 ± 0.477
0.533IleMet: 0.533 ± 0.156
0.906IleAsn: 0.906 ± 0.184
2.397IlePro: 2.397 ± 0.381
1.438IleGln: 1.438 ± 0.343
3.463IleArg: 3.463 ± 0.457
1.545IleSer: 1.545 ± 0.296
3.409IleThr: 3.409 ± 0.45
3.303IleVal: 3.303 ± 0.457
0.639IleTrp: 0.639 ± 0.166
0.852IleTyr: 0.852 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
3.409LysAla: 3.409 ± 0.517
0.0LysCys: 0.0 ± 0.0
1.971LysAsp: 1.971 ± 0.438
1.278LysGlu: 1.278 ± 0.364
0.746LysPhe: 0.746 ± 0.227
1.705LysGly: 1.705 ± 0.354
0.533LysHis: 0.533 ± 0.187
1.172LysIle: 1.172 ± 0.259
1.332LysLys: 1.332 ± 0.398
2.823LysLeu: 2.823 ± 0.392
0.426LysMet: 0.426 ± 0.156
0.799LysAsn: 0.799 ± 0.18
1.811LysPro: 1.811 ± 0.386
0.852LysGln: 0.852 ± 0.214
2.823LysArg: 2.823 ± 0.369
1.705LysSer: 1.705 ± 0.296
2.024LysThr: 2.024 ± 0.362
1.918LysVal: 1.918 ± 0.3
0.426LysTrp: 0.426 ± 0.134
0.533LysTyr: 0.533 ± 0.157
0.0LysXaa: 0.0 ± 0.0
Leu
10.974LeuAla: 10.974 ± 0.889
0.586LeuCys: 0.586 ± 0.187
7.138LeuAsp: 7.138 ± 0.717
3.942LeuGlu: 3.942 ± 0.455
1.705LeuPhe: 1.705 ± 0.364
7.192LeuGly: 7.192 ± 1.023
1.545LeuHis: 1.545 ± 0.308
3.516LeuIle: 3.516 ± 0.451
2.237LeuLys: 2.237 ± 0.449
5.7LeuLeu: 5.7 ± 0.609
1.225LeuMet: 1.225 ± 0.248
1.758LeuAsn: 1.758 ± 0.271
4.262LeuPro: 4.262 ± 0.438
2.717LeuGln: 2.717 ± 0.516
5.807LeuArg: 5.807 ± 0.556
4.688LeuSer: 4.688 ± 0.517
5.753LeuThr: 5.753 ± 0.532
5.647LeuVal: 5.647 ± 0.564
2.397LeuTrp: 2.397 ± 0.375
1.598LeuTyr: 1.598 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
1.918MetAla: 1.918 ± 0.319
0.266MetCys: 0.266 ± 0.109
0.906MetAsp: 0.906 ± 0.229
0.639MetGlu: 0.639 ± 0.204
0.586MetPhe: 0.586 ± 0.184
1.438MetGly: 1.438 ± 0.228
0.16MetHis: 0.16 ± 0.087
0.852MetIle: 0.852 ± 0.267
0.533MetLys: 0.533 ± 0.16
1.278MetLeu: 1.278 ± 0.312
0.16MetMet: 0.16 ± 0.097
0.586MetAsn: 0.586 ± 0.207
1.545MetPro: 1.545 ± 0.286
0.693MetGln: 0.693 ± 0.201
1.758MetArg: 1.758 ± 0.289
1.705MetSer: 1.705 ± 0.317
3.25MetThr: 3.25 ± 0.41
1.119MetVal: 1.119 ± 0.254
0.426MetTrp: 0.426 ± 0.143
0.479MetTyr: 0.479 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
3.569AsnAla: 3.569 ± 0.471
0.426AsnCys: 0.426 ± 0.155
1.545AsnAsp: 1.545 ± 0.284
1.758AsnGlu: 1.758 ± 0.302
0.639AsnPhe: 0.639 ± 0.177
3.036AsnGly: 3.036 ± 0.379
0.533AsnHis: 0.533 ± 0.155
0.852AsnIle: 0.852 ± 0.183
0.852AsnLys: 0.852 ± 0.221
2.291AsnLeu: 2.291 ± 0.461
0.373AsnMet: 0.373 ± 0.125
0.586AsnAsn: 0.586 ± 0.161
2.61AsnPro: 2.61 ± 0.325
0.586AsnGln: 0.586 ± 0.191
1.971AsnArg: 1.971 ± 0.307
1.225AsnSer: 1.225 ± 0.237
1.758AsnThr: 1.758 ± 0.338
2.397AsnVal: 2.397 ± 0.396
0.32AsnTrp: 0.32 ± 0.122
0.373AsnTyr: 0.373 ± 0.14
0.0AsnXaa: 0.0 ± 0.0
Pro
8.204ProAla: 8.204 ± 0.843
0.639ProCys: 0.639 ± 0.216
5.274ProAsp: 5.274 ± 0.706
3.995ProGlu: 3.995 ± 0.558
1.758ProPhe: 1.758 ± 0.365
5.647ProGly: 5.647 ± 0.67
1.438ProHis: 1.438 ± 0.451
3.143ProIle: 3.143 ± 0.356
1.864ProLys: 1.864 ± 0.349
3.516ProLeu: 3.516 ± 0.399
1.065ProMet: 1.065 ± 0.23
1.492ProAsn: 1.492 ± 0.319
4.421ProPro: 4.421 ± 0.87
1.492ProGln: 1.492 ± 0.295
3.782ProArg: 3.782 ± 0.464
3.622ProSer: 3.622 ± 0.421
4.848ProThr: 4.848 ± 0.604
4.794ProVal: 4.794 ± 0.5
1.172ProTrp: 1.172 ± 0.319
1.065ProTyr: 1.065 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
4.581GlnAla: 4.581 ± 0.524
0.32GlnCys: 0.32 ± 0.146
1.598GlnAsp: 1.598 ± 0.351
1.545GlnGlu: 1.545 ± 0.28
0.799GlnPhe: 0.799 ± 0.211
2.344GlnGly: 2.344 ± 0.377
1.065GlnHis: 1.065 ± 0.209
1.705GlnIle: 1.705 ± 0.312
1.012GlnLys: 1.012 ± 0.281
2.823GlnLeu: 2.823 ± 0.396
1.225GlnMet: 1.225 ± 0.207
0.852GlnAsn: 0.852 ± 0.226
1.971GlnPro: 1.971 ± 0.369
1.598GlnGln: 1.598 ± 0.331
3.036GlnArg: 3.036 ± 0.376
1.705GlnSer: 1.705 ± 0.34
1.811GlnThr: 1.811 ± 0.354
2.237GlnVal: 2.237 ± 0.327
0.639GlnTrp: 0.639 ± 0.159
0.586GlnTyr: 0.586 ± 0.182
0.0GlnXaa: 0.0 ± 0.0
Arg
9.482ArgAla: 9.482 ± 0.78
1.012ArgCys: 1.012 ± 0.255
5.753ArgAsp: 5.753 ± 0.667
4.155ArgGlu: 4.155 ± 0.502
1.545ArgPhe: 1.545 ± 0.317
5.487ArgGly: 5.487 ± 0.597
1.918ArgHis: 1.918 ± 0.419
2.557ArgIle: 2.557 ± 0.352
2.823ArgLys: 2.823 ± 0.47
5.7ArgLeu: 5.7 ± 0.587
2.557ArgMet: 2.557 ± 0.395
1.811ArgAsn: 1.811 ± 0.32
3.622ArgPro: 3.622 ± 0.735
2.983ArgGln: 2.983 ± 0.443
7.884ArgArg: 7.884 ± 0.958
3.516ArgSer: 3.516 ± 0.494
4.208ArgThr: 4.208 ± 0.578
4.635ArgVal: 4.635 ± 0.711
1.758ArgTrp: 1.758 ± 0.357
1.332ArgTyr: 1.332 ± 0.267
0.0ArgXaa: 0.0 ± 0.0
Ser
6.446SerAla: 6.446 ± 0.819
0.266SerCys: 0.266 ± 0.139
3.463SerAsp: 3.463 ± 0.454
2.237SerGlu: 2.237 ± 0.305
1.278SerPhe: 1.278 ± 0.249
5.966SerGly: 5.966 ± 0.819
0.746SerHis: 0.746 ± 0.224
2.504SerIle: 2.504 ± 0.32
1.065SerLys: 1.065 ± 0.237
4.315SerLeu: 4.315 ± 0.473
1.278SerMet: 1.278 ± 0.242
1.119SerAsn: 1.119 ± 0.231
3.09SerPro: 3.09 ± 0.462
1.225SerGln: 1.225 ± 0.189
2.93SerArg: 2.93 ± 0.34
2.557SerSer: 2.557 ± 0.508
4.528SerThr: 4.528 ± 0.514
3.622SerVal: 3.622 ± 0.461
1.278SerTrp: 1.278 ± 0.266
1.172SerTyr: 1.172 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
9.376ThrAla: 9.376 ± 1.292
0.479ThrCys: 0.479 ± 0.205
4.421ThrAsp: 4.421 ± 0.498
4.155ThrGlu: 4.155 ± 0.561
1.918ThrPhe: 1.918 ± 0.315
7.032ThrGly: 7.032 ± 0.701
1.119ThrHis: 1.119 ± 0.294
3.463ThrIle: 3.463 ± 0.449
1.705ThrLys: 1.705 ± 0.312
4.794ThrLeu: 4.794 ± 0.515
1.278ThrMet: 1.278 ± 0.246
1.971ThrAsn: 1.971 ± 0.308
5.274ThrPro: 5.274 ± 0.477
1.598ThrGln: 1.598 ± 0.286
4.635ThrArg: 4.635 ± 0.476
3.995ThrSer: 3.995 ± 0.452
5.061ThrThr: 5.061 ± 0.698
5.114ThrVal: 5.114 ± 0.624
1.278ThrTrp: 1.278 ± 0.233
1.225ThrTyr: 1.225 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
7.511ValAla: 7.511 ± 0.617
0.693ValCys: 0.693 ± 0.203
5.7ValAsp: 5.7 ± 0.491
4.315ValGlu: 4.315 ± 0.445
1.492ValPhe: 1.492 ± 0.344
5.807ValGly: 5.807 ± 0.7
1.598ValHis: 1.598 ± 0.323
3.463ValIle: 3.463 ± 0.438
1.651ValLys: 1.651 ± 0.279
6.925ValLeu: 6.925 ± 0.652
1.012ValMet: 1.012 ± 0.216
2.45ValAsn: 2.45 ± 0.401
5.167ValPro: 5.167 ± 0.571
2.237ValGln: 2.237 ± 0.461
5.007ValArg: 5.007 ± 0.584
4.368ValSer: 4.368 ± 0.522
5.7ValThr: 5.7 ± 0.711
6.552ValVal: 6.552 ± 0.513
1.864ValTrp: 1.864 ± 0.356
1.385ValTyr: 1.385 ± 0.29
0.0ValXaa: 0.0 ± 0.0
Trp
2.131TrpAla: 2.131 ± 0.344
0.373TrpCys: 0.373 ± 0.149
1.332TrpAsp: 1.332 ± 0.257
1.012TrpGlu: 1.012 ± 0.242
0.533TrpPhe: 0.533 ± 0.206
1.119TrpGly: 1.119 ± 0.213
0.693TrpHis: 0.693 ± 0.176
0.746TrpIle: 0.746 ± 0.24
0.479TrpLys: 0.479 ± 0.168
2.45TrpLeu: 2.45 ± 0.412
0.533TrpMet: 0.533 ± 0.173
0.852TrpAsn: 0.852 ± 0.214
1.385TrpPro: 1.385 ± 0.333
0.959TrpGln: 0.959 ± 0.216
1.012TrpArg: 1.012 ± 0.249
1.278TrpSer: 1.278 ± 0.289
1.598TrpThr: 1.598 ± 0.3
1.811TrpVal: 1.811 ± 0.293
0.639TrpTrp: 0.639 ± 0.173
0.266TrpTyr: 0.266 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.811TyrAla: 1.811 ± 0.449
0.213TyrCys: 0.213 ± 0.111
1.438TyrAsp: 1.438 ± 0.309
1.119TyrGlu: 1.119 ± 0.302
0.693TyrPhe: 0.693 ± 0.207
2.504TyrGly: 2.504 ± 0.372
0.586TyrHis: 0.586 ± 0.184
0.213TyrIle: 0.213 ± 0.143
0.533TyrLys: 0.533 ± 0.168
1.119TyrLeu: 1.119 ± 0.242
0.373TyrMet: 0.373 ± 0.14
0.746TyrAsn: 0.746 ± 0.207
1.065TyrPro: 1.065 ± 0.235
0.746TyrGln: 0.746 ± 0.221
1.438TyrArg: 1.438 ± 0.304
1.065TyrSer: 1.065 ± 0.216
1.864TyrThr: 1.864 ± 0.347
1.332TyrVal: 1.332 ± 0.258
0.426TyrTrp: 0.426 ± 0.153
0.426TyrTyr: 0.426 ± 0.166
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (18773 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski