Crane-associated adenovirus 1
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5H2X6N2|A0A5H2X6N2_9ADEN Terminal protein pTP OS=Crane-associated adenovirus 1 OX=2559941 GN=pTP PE=4 SV=1
MM1 pKa = 8.03 DD2 pKa = 4.73 LQADD6 pKa = 3.9 EE7 pKa = 4.89 EE8 pKa = 4.51 MIPGNEE14 pKa = 3.97 EE15 pKa = 3.61 EE16 pKa = 5.02 VDD18 pKa = 3.56 YY19 pKa = 10.92 LLNMDD24 pKa = 5.13 LDD26 pKa = 4.3 LDD28 pKa = 3.65 IRR30 pKa = 11.84 EE31 pKa = 4.1 MMMFSTLQLLDD42 pKa = 3.74 YY43 pKa = 10.52 EE44 pKa = 4.49 NFSVSVDD51 pKa = 3.35 PCSCHH56 pKa = 6.07 AQSIKK61 pKa = 10.57 NFGMSFHH68 pKa = 6.8 TNADD72 pKa = 3.59 SEE74 pKa = 4.66 IYY76 pKa = 10.08 PLLCSALEE84 pKa = 4.11 LDD86 pKa = 4.67 FSVAGTYY93 pKa = 9.68 FVVNSVGSMFTSFAPTFFEE112 pKa = 4.81 KK113 pKa = 10.46 KK114 pKa = 9.47 VEE116 pKa = 4.25 SARR119 pKa = 11.84 GMLINVTPDD128 pKa = 2.83 KK129 pKa = 11.2 QNNFLKK135 pKa = 10.81 CIAQIGDD142 pKa = 3.99 LGLVPCDD149 pKa = 3.49 YY150 pKa = 10.92 LRR152 pKa = 11.84 EE153 pKa = 4.14 IDD155 pKa = 3.97 VDD157 pKa = 3.59 TDD159 pKa = 3.92 SEE161 pKa = 4.41 IVRR164 pKa = 11.84 MLVKK168 pKa = 10.34 EE169 pKa = 4.23 WFEE172 pKa = 3.85 VAAVIIVHH180 pKa = 4.84 WQEE183 pKa = 3.99 SKK185 pKa = 10.85 VASLINN191 pKa = 3.51
Molecular weight: 21.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.05
IPC2_protein 4.062
IPC_protein 4.024
Toseland 3.834
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.897
Rodwell 3.846
Grimsley 3.745
Solomon 3.986
Lehninger 3.935
Nozaki 4.101
DTASelect 4.291
Thurlkill 3.859
EMBOSS 3.91
Sillero 4.139
Patrickios 3.007
IPC_peptide 3.986
IPC2_peptide 4.126
IPC2.peptide.svr19 4.038
Protein with the highest isoelectric point:
>tr|A0A5H2WW01|A0A5H2WW01_9ADEN U exon OS=Crane-associated adenovirus 1 OX=2559941 GN=U PE=4 SV=1
MM1 pKa = 7.65 SILVSPSDD9 pKa = 3.44 NRR11 pKa = 11.84 GWGVRR16 pKa = 11.84 RR17 pKa = 11.84 CRR19 pKa = 11.84 RR20 pKa = 11.84 GIRR23 pKa = 11.84 GVGCRR28 pKa = 11.84 RR29 pKa = 11.84 GLTLRR34 pKa = 11.84 GLLGMGARR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 VRR46 pKa = 11.84 RR47 pKa = 11.84 SRR49 pKa = 11.84 ARR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 APPVSRR59 pKa = 11.84 TTLVAVRR66 pKa = 11.84 STRR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 3.21
Molecular weight: 8.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 10.95
IPC_protein 12.559
Toseland 12.72
ProMoST 13.217
Dawson 12.72
Bjellqvist 12.72
Wikipedia 13.203
Rodwell 12.223
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.72
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 11.945
IPC_peptide 13.217
IPC2_peptide 12.208
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
9287
73
1206
403.8
45.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.588 ± 0.292
1.798 ± 0.293
5.61 ± 0.249
5.739 ± 0.433
4.469 ± 0.255
4.824 ± 0.36
2.014 ± 0.157
6.967 ± 0.261
5.545 ± 0.703
8.463 ± 0.366
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.66 ± 0.206
7.161 ± 0.37
5.502 ± 0.413
3.876 ± 0.237
5.19 ± 0.597
8.431 ± 0.391
5.578 ± 0.24
5.169 ± 0.208
1.152 ± 0.115
4.264 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here