Pasteurella virus F108
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1I0Z7|Q1I0Z7_9CAUD Uncharacterized protein OS=Pasteurella virus F108 OX=342564 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.57 KK3 pKa = 9.13 MLYY6 pKa = 9.26 QQLTEE11 pKa = 4.31 FLLTKK16 pKa = 10.69 LPARR20 pKa = 11.84 YY21 pKa = 8.38 HH22 pKa = 5.47 AHH24 pKa = 5.03 FHH26 pKa = 5.75 AWIEE30 pKa = 4.03 NGKK33 pKa = 9.32 LLNQGKK39 pKa = 9.24 RR40 pKa = 11.84 VTDD43 pKa = 3.59 AGIEE47 pKa = 4.08 IAHH50 pKa = 6.12 IQYY53 pKa = 10.75 DD54 pKa = 3.52 AVLFFDD60 pKa = 4.14 EE61 pKa = 5.46 FPFRR65 pKa = 11.84 EE66 pKa = 3.93 ISAQKK71 pKa = 10.37 IMADD75 pKa = 3.03 IQIWLNEE82 pKa = 3.7 NDD84 pKa = 3.8 YY85 pKa = 11.41 LRR87 pKa = 11.84 DD88 pKa = 3.58 VLDD91 pKa = 4.25 EE92 pKa = 4.34 YY93 pKa = 9.79 EE94 pKa = 4.47 TPFEE98 pKa = 4.57 LEE100 pKa = 4.3 IINDD104 pKa = 3.85 DD105 pKa = 3.89 VADD108 pKa = 3.86 LTFTISFQEE117 pKa = 4.16 PLTAVEE123 pKa = 5.34 DD124 pKa = 4.12 DD125 pKa = 4.55 AGDD128 pKa = 4.37 LEE130 pKa = 5.06 IDD132 pKa = 3.69 SKK134 pKa = 11.25 KK135 pKa = 10.62 YY136 pKa = 10.2 RR137 pKa = 11.84 LDD139 pKa = 3.89 EE140 pKa = 4.07 IEE142 pKa = 4.05 INIVNSIDD150 pKa = 3.71 LVYY153 pKa = 9.98 PAPKK157 pKa = 9.25 QAKK160 pKa = 8.69 EE161 pKa = 3.91 DD162 pKa = 3.73 NAAQTMTHH170 pKa = 6.15
Molecular weight: 19.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.438
IPC2_protein 4.38
IPC_protein 4.329
Toseland 4.151
ProMoST 4.431
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.177
Rodwell 4.164
Grimsley 4.062
Solomon 4.291
Lehninger 4.24
Nozaki 4.393
DTASelect 4.584
Thurlkill 4.177
EMBOSS 4.202
Sillero 4.444
Patrickios 3.757
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.361
Protein with the highest isoelectric point:
>tr|Q1I0Z6|Q1I0Z6_9CAUD Uncharacterized protein OS=Pasteurella virus F108 OX=342564 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 9.54 PAIKK6 pKa = 9.9 MGVNPDD12 pKa = 3.78 DD13 pKa = 5.23 LRR15 pKa = 11.84 DD16 pKa = 3.76 FLKK19 pKa = 10.9 DD20 pKa = 3.6 LEE22 pKa = 4.5 LLKK25 pKa = 10.56 IPPKK29 pKa = 9.78 KK30 pKa = 10.1 KK31 pKa = 10.2 KK32 pKa = 10.04 EE33 pKa = 3.72 ILIRR37 pKa = 11.84 TLQEE41 pKa = 3.55 MKK43 pKa = 10.39 KK44 pKa = 10.09 RR45 pKa = 11.84 SVKK48 pKa = 10.19 SASNQRR54 pKa = 11.84 TPTGSGWKK62 pKa = 9.14 PRR64 pKa = 11.84 KK65 pKa = 9.69 NGNAKK70 pKa = 8.38 MLRR73 pKa = 11.84 RR74 pKa = 11.84 IAKK77 pKa = 6.93 TLKK80 pKa = 8.79 FTSADD85 pKa = 3.75 RR86 pKa = 11.84 EE87 pKa = 4.26 IKK89 pKa = 10.1 RR90 pKa = 11.84 VCTISRR96 pKa = 11.84 NAQRR100 pKa = 11.84 RR101 pKa = 11.84 SQKK104 pKa = 8.31 EE105 pKa = 3.7 HH106 pKa = 5.25 QRR108 pKa = 11.84 GAKK111 pKa = 7.75 ITNLKK116 pKa = 10.47 SVILRR121 pKa = 11.84 KK122 pKa = 9.87 SRR124 pKa = 11.84 AGTAKK129 pKa = 10.61 DD130 pKa = 3.45 PATMRR135 pKa = 11.84 QAKK138 pKa = 9.65 KK139 pKa = 10.61 LRR141 pKa = 11.84 DD142 pKa = 3.46 LGYY145 pKa = 8.55 TVPNGTTKK153 pKa = 10.4 SGKK156 pKa = 9.11 KK157 pKa = 9.49 RR158 pKa = 11.84 YY159 pKa = 8.89 RR160 pKa = 11.84 RR161 pKa = 11.84 PSARR165 pKa = 11.84 EE166 pKa = 3.32 IVATLSRR173 pKa = 11.84 AKK175 pKa = 10.5 ASLLIRR181 pKa = 11.84 YY182 pKa = 7.47 FQEE185 pKa = 3.82 KK186 pKa = 9.93 EE187 pKa = 3.67 EE188 pKa = 4.21 RR189 pKa = 11.84 QGKK192 pKa = 9.28 RR193 pKa = 11.84 LTKK196 pKa = 10.04 WIIPTEE202 pKa = 3.87 KK203 pKa = 10.44 RR204 pKa = 11.84 PFLDD208 pKa = 4.34 EE209 pKa = 4.05 RR210 pKa = 11.84 DD211 pKa = 3.76 KK212 pKa = 11.8 EE213 pKa = 4.02 NAEE216 pKa = 3.83 ILKK219 pKa = 10.25 EE220 pKa = 4.26 FILKK224 pKa = 10.6 FSGIEE229 pKa = 3.88 KK230 pKa = 10.5
Molecular weight: 26.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.27
IPC2_protein 9.853
IPC_protein 10.526
Toseland 11.184
ProMoST 10.95
Dawson 11.228
Bjellqvist 10.935
Wikipedia 11.447
Rodwell 11.579
Grimsley 11.242
Solomon 11.418
Lehninger 11.374
Nozaki 11.155
DTASelect 10.935
Thurlkill 11.169
EMBOSS 11.594
Sillero 11.169
Patrickios 11.286
IPC_peptide 11.433
IPC2_peptide 9.633
IPC2.peptide.svr19 8.744
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
8713
50
769
198.0
22.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.977 ± 0.391
0.976 ± 0.161
5.406 ± 0.276
6.84 ± 0.399
4.648 ± 0.237
6.071 ± 0.394
1.722 ± 0.175
7.437 ± 0.337
8.252 ± 0.41
8.344 ± 0.255
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.192 ± 0.158
5.704 ± 0.253
3.133 ± 0.213
4.292 ± 0.23
5.36 ± 0.385
5.807 ± 0.33
5.704 ± 0.24
5.784 ± 0.38
1.228 ± 0.14
3.122 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here