Pontibacillus marinus BH030004 = DSM 16465

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pontibacillus; Pontibacillus marinus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A5I139|A0A0A5I139_9BACI Uncharacterized protein OS=Pontibacillus marinus BH030004 = DSM 16465 OX=1385511 GN=N783_05610 PE=4 SV=1
TT1 pKa = 7.33GPTGPTGEE9 pKa = 4.61CVCLPRR15 pKa = 11.84YY16 pKa = 9.72CDD18 pKa = 4.46FYY20 pKa = 11.77FEE22 pKa = 4.36GTTINVAPNSSIPFNEE38 pKa = 4.13EE39 pKa = 4.02GEE41 pKa = 4.39CTEE44 pKa = 6.25DD45 pKa = 4.85DD46 pKa = 3.88FTPVNVMEE54 pKa = 4.57GTFTQIEE61 pKa = 4.49VEE63 pKa = 4.18HH64 pKa = 6.58EE65 pKa = 3.69GDD67 pKa = 3.56YY68 pKa = 11.05LINYY72 pKa = 8.16SVIFNNTPPTEE83 pKa = 4.05IVEE86 pKa = 4.37GAFALFVDD94 pKa = 4.81GLDD97 pKa = 3.33VDD99 pKa = 4.22ASRR102 pKa = 11.84FGEE105 pKa = 4.42TIVLASQPAGAQTKK119 pKa = 9.39IQVNGEE125 pKa = 4.37AIVHH129 pKa = 5.7IPEE132 pKa = 4.51GGILEE137 pKa = 4.57LRR139 pKa = 11.84NISNRR144 pKa = 11.84TVHH147 pKa = 5.87VLASVAGADD156 pKa = 3.44INGAAITIVQLDD168 pKa = 3.82NDD170 pKa = 4.47TVV172 pKa = 3.26

Molecular weight: 18.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A5FT77|A0A0A5FT77_9BACI ABC transporter substrate-binding protein OS=Pontibacillus marinus BH030004 = DSM 16465 OX=1385511 GN=N783_20170 PE=4 SV=1
GG1 pKa = 7.08GGGIFAWVRR10 pKa = 11.84AAFARR15 pKa = 11.84VRR17 pKa = 11.84SVFARR22 pKa = 11.84VRR24 pKa = 11.84AAFARR29 pKa = 11.84VRR31 pKa = 11.84AAFARR36 pKa = 11.84VRR38 pKa = 11.84GAFAWVRR45 pKa = 11.84SAFARR50 pKa = 11.84VRR52 pKa = 11.84AVFARR57 pKa = 11.84VRR59 pKa = 11.84AAFAQVQGAFARR71 pKa = 11.84VRR73 pKa = 11.84AAFARR78 pKa = 11.84VCAAFARR85 pKa = 11.84VRR87 pKa = 11.84AAFARR92 pKa = 11.84VRR94 pKa = 11.84AGFARR99 pKa = 11.84VRR101 pKa = 11.84AAFARR106 pKa = 11.84VRR108 pKa = 11.84SAFARR113 pKa = 11.84VRR115 pKa = 11.84TTFAA119 pKa = 3.75

Molecular weight: 13.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4252

0

4252

1200615

26

3060

282.4

31.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.345 ± 0.043

0.634 ± 0.012

5.557 ± 0.036

7.975 ± 0.042

4.507 ± 0.032

6.809 ± 0.037

2.274 ± 0.022

7.35 ± 0.043

6.674 ± 0.039

9.417 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.897 ± 0.018

4.505 ± 0.033

3.65 ± 0.021

4.252 ± 0.035

3.891 ± 0.028

6.176 ± 0.035

5.329 ± 0.03

7.014 ± 0.03

1.085 ± 0.015

3.658 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski