Lasius niger (Black garden ant)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea; Formicidae;

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18044 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J7MMS0|A0A0J7MMS0_LASNI Ubiquitin carboxyl-terminal hydrolase OS=Lasius niger OX=67767 GN=RF55_24823 PE=4 SV=1
PP1 pKa = 7.55SVDD4 pKa = 3.41CSKK7 pKa = 11.52DD8 pKa = 3.67DD9 pKa = 4.77GDD11 pKa = 6.07SDD13 pKa = 6.38DD14 pKa = 6.54DD15 pKa = 6.65DD16 pKa = 7.52DD17 pKa = 7.61DD18 pKa = 7.4DD19 pKa = 7.61DD20 pKa = 6.67DD21 pKa = 7.07DD22 pKa = 4.24EE23 pKa = 5.74VEE25 pKa = 4.38TGAVEE30 pKa = 3.95YY31 pKa = 10.98APVKK35 pKa = 10.44CVSAYY40 pKa = 8.5PKK42 pKa = 11.08LMGTSCWPSTHH53 pKa = 6.67IPTGYY58 pKa = 9.77QYY60 pKa = 11.31HH61 pKa = 7.53DD62 pKa = 4.06GYY64 pKa = 10.94MPDD67 pKa = 3.15QLEE70 pKa = 4.54RR71 pKa = 11.84ILDD74 pKa = 3.59ISQRR78 pKa = 3.57

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J7JXI5|A0A0J7JXI5_LASNI CCHC-type domain-containing protein (Fragment) OS=Lasius niger OX=67767 GN=RF55_21751 PE=4 SV=1
LL1 pKa = 7.42SLTIIIITTTKK12 pKa = 7.99TTTTTTITTRR22 pKa = 11.84ITTRR26 pKa = 11.84ITITKK31 pKa = 8.2TKK33 pKa = 9.84TRR35 pKa = 11.84TTTRR39 pKa = 11.84TRR41 pKa = 11.84TTRR44 pKa = 11.84TTRR47 pKa = 11.84TTKK50 pKa = 9.77RR51 pKa = 11.84TTKK54 pKa = 9.73RR55 pKa = 11.84TTTRR59 pKa = 11.84TTTTRR64 pKa = 11.84TTTMRR69 pKa = 11.84TTTTRR74 pKa = 11.84TRR76 pKa = 11.84TRR78 pKa = 11.84TTRR81 pKa = 11.84TRR83 pKa = 11.84TTRR86 pKa = 11.84TTKK89 pKa = 9.42RR90 pKa = 11.84TTRR93 pKa = 11.84KK94 pKa = 5.14TTTRR98 pKa = 11.84TTTRR102 pKa = 11.84TTTTRR107 pKa = 11.84STTKK111 pKa = 9.71TIFFIAYY118 pKa = 9.01SCC120 pKa = 4.19

Molecular weight: 13.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18044

0

18044

6832129

8

7071

378.6

42.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.694 ± 0.02

1.77 ± 0.013

5.722 ± 0.014

7.186 ± 0.026

3.377 ± 0.012

5.629 ± 0.027

2.411 ± 0.01

5.779 ± 0.016

6.62 ± 0.026

8.946 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.009

4.87 ± 0.019

4.899 ± 0.021

4.181 ± 0.018

6.053 ± 0.019

7.629 ± 0.026

5.846 ± 0.017

6.015 ± 0.014

1.143 ± 0.007

2.942 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski