Streptomyces sp. F-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K2FK26|A0A1K2FK26_9ACTN Non-specific serine/threonine protein kinase OS=Streptomyces sp. F-1 OX=463642 GN=afsK_4 PE=4 SV=1
MM1 pKa = 6.63QQEE4 pKa = 4.28APAGEE9 pKa = 4.22ALEE12 pKa = 4.18VWIDD16 pKa = 3.41QDD18 pKa = 3.99LCTGDD23 pKa = 5.01GICAQYY29 pKa = 10.9APEE32 pKa = 4.23VFEE35 pKa = 5.9LDD37 pKa = 3.05IDD39 pKa = 3.65GLAYY43 pKa = 10.7VKK45 pKa = 10.83GADD48 pKa = 4.69DD49 pKa = 4.63EE50 pKa = 4.64LLQEE54 pKa = 4.73AGATVPVPVPLLTDD68 pKa = 3.5VVDD71 pKa = 4.29SARR74 pKa = 11.84EE75 pKa = 3.94CPGEE79 pKa = 4.38CIHH82 pKa = 6.36VRR84 pKa = 11.84RR85 pKa = 11.84VLDD88 pKa = 3.47RR89 pKa = 11.84VEE91 pKa = 4.69VYY93 pKa = 10.9GPDD96 pKa = 3.45AEE98 pKa = 4.31

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K2FY91|A0A1K2FY91_9ACTN S-adenosyl-L-methionine-dependent methyltransferase OS=Streptomyces sp. F-1 OX=463642 GN=STEPF1_05940 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.11VRR4 pKa = 11.84RR5 pKa = 11.84SLRR8 pKa = 11.84SLKK11 pKa = 10.33AKK13 pKa = 10.3PGAQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GVTFVINKK30 pKa = 7.29KK31 pKa = 9.86HH32 pKa = 5.85PRR34 pKa = 11.84FKK36 pKa = 10.84ARR38 pKa = 11.84QSS40 pKa = 3.18

Molecular weight: 4.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7251

0

7251

2351009

29

5701

324.2

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.97 ± 0.043

0.816 ± 0.009

5.918 ± 0.027

5.615 ± 0.029

2.674 ± 0.018

9.581 ± 0.031

2.389 ± 0.015

2.928 ± 0.021

1.999 ± 0.025

10.471 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.664 ± 0.013

1.657 ± 0.014

6.321 ± 0.026

2.671 ± 0.018

8.466 ± 0.035

4.758 ± 0.022

6.109 ± 0.023

8.431 ± 0.026

1.495 ± 0.012

2.064 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski