Amino acid dipepetide frequency for Streptomyces sp. F-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.549AlaAla: 22.549 ± 0.132	1.1AlaCys: 1.1 ± 0.023	8.607AlaAsp: 8.607 ± 0.059	9.025AlaGlu: 9.025 ± 0.097	3.55AlaPhe: 3.55 ± 0.041	13.817AlaGly: 13.817 ± 0.099	3.159AlaHis: 3.159 ± 0.037	3.205AlaIle: 3.205 ± 0.044	2.682AlaLys: 2.682 ± 0.046	15.138AlaLeu: 15.138 ± 0.114	2.348AlaMet: 2.348 ± 0.035	1.88AlaAsn: 1.88 ± 0.032	7.774AlaPro: 7.774 ± 0.09	3.733AlaGln: 3.733 ± 0.045	11.524AlaArg: 11.524 ± 0.097	5.472AlaSer: 5.472 ± 0.051	6.972AlaThr: 6.972 ± 0.058	12.563AlaVal: 12.563 ± 0.087	1.867AlaTrp: 1.867 ± 0.027	2.738AlaTyr: 2.738 ± 0.041	0.001AlaXaa: 0.001 ± 0.001
Cys	1.193CysAla: 1.193 ± 0.025	0.102CysCys: 0.102 ± 0.008	0.479CysAsp: 0.479 ± 0.015	0.387CysGlu: 0.387 ± 0.014	0.24CysPhe: 0.24 ± 0.01	0.991CysGly: 0.991 ± 0.024	0.217CysHis: 0.217 ± 0.009	0.155CysIle: 0.155 ± 0.007	0.106CysLys: 0.106 ± 0.007	0.785CysLeu: 0.785 ± 0.019	0.128CysMet: 0.128 ± 0.008	0.136CysAsn: 0.136 ± 0.009	0.527CysPro: 0.527 ± 0.019	0.158CysGln: 0.158 ± 0.009	0.639CysArg: 0.639 ± 0.018	0.414CysSer: 0.414 ± 0.015	0.501CysThr: 0.501 ± 0.016	0.709CysVal: 0.709 ± 0.016	0.131CysTrp: 0.131 ± 0.008	0.158CysTyr: 0.158 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.744AspAla: 7.744 ± 0.071	0.416AspCys: 0.416 ± 0.015	3.481AspAsp: 3.481 ± 0.04	3.705AspGlu: 3.705 ± 0.047	1.598AspPhe: 1.598 ± 0.027	6.428AspGly: 6.428 ± 0.058	1.447AspHis: 1.447 ± 0.026	1.789AspIle: 1.789 ± 0.026	1.125AspLys: 1.125 ± 0.028	6.148AspLeu: 6.148 ± 0.05	0.831AspMet: 0.831 ± 0.019	0.921AspAsn: 0.921 ± 0.021	4.636AspPro: 4.636 ± 0.043	1.49AspGln: 1.49 ± 0.027	5.154AspArg: 5.154 ± 0.054	2.312AspSer: 2.312 ± 0.036	3.216AspThr: 3.216 ± 0.041	4.618AspVal: 4.618 ± 0.048	1.042AspTrp: 1.042 ± 0.027	1.079AspTyr: 1.079 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.331GluAla: 7.331 ± 0.084	0.363GluCys: 0.363 ± 0.013	2.823GluAsp: 2.823 ± 0.04	3.647GluGlu: 3.647 ± 0.05	1.435GluPhe: 1.435 ± 0.027	4.184GluGly: 4.184 ± 0.046	1.591GluHis: 1.591 ± 0.027	2.104GluIle: 2.104 ± 0.034	1.297GluLys: 1.297 ± 0.027	6.884GluLeu: 6.884 ± 0.071	0.852GluMet: 0.852 ± 0.018	0.961GluAsn: 0.961 ± 0.024	3.429GluPro: 3.429 ± 0.046	2.201GluGln: 2.201 ± 0.032	5.739GluArg: 5.739 ± 0.064	2.285GluSer: 2.285 ± 0.031	2.863GluThr: 2.863 ± 0.035	4.357GluVal: 4.357 ± 0.047	0.723GluTrp: 0.723 ± 0.017	1.08GluTyr: 1.08 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.693PheAla: 3.693 ± 0.042	0.287PheCys: 0.287 ± 0.011	1.889PheAsp: 1.889 ± 0.033	1.42PheGlu: 1.42 ± 0.027	0.877PhePhe: 0.877 ± 0.026	2.96PheGly: 2.96 ± 0.036	0.644PheHis: 0.644 ± 0.016	0.638PheIle: 0.638 ± 0.018	0.462PheLys: 0.462 ± 0.016	2.618PheLeu: 2.618 ± 0.041	0.382PheMet: 0.382 ± 0.014	0.522PheAsn: 0.522 ± 0.016	1.379PhePro: 1.379 ± 0.023	0.655PheGln: 0.655 ± 0.016	1.876PheArg: 1.876 ± 0.028	1.404PheSer: 1.404 ± 0.026	1.96PheThr: 1.96 ± 0.034	2.148PheVal: 2.148 ± 0.037	0.396PheTrp: 0.396 ± 0.013	0.533PheTyr: 0.533 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.593GlyAla: 11.593 ± 0.095	0.865GlyCys: 0.865 ± 0.017	5.126GlyAsp: 5.126 ± 0.052	5.135GlyGlu: 5.135 ± 0.049	2.853GlyPhe: 2.853 ± 0.042	8.997GlyGly: 8.997 ± 0.087	2.536GlyHis: 2.536 ± 0.036	3.245GlyIle: 3.245 ± 0.039	2.331GlyLys: 2.331 ± 0.04	9.482GlyLeu: 9.482 ± 0.073	1.971GlyMet: 1.971 ± 0.031	1.673GlyAsn: 1.673 ± 0.034	5.491GlyPro: 5.491 ± 0.063	2.624GlyGln: 2.624 ± 0.038	8.436GlyArg: 8.436 ± 0.077	5.23GlySer: 5.23 ± 0.061	6.695GlyThr: 6.695 ± 0.072	7.454GlyVal: 7.454 ± 0.071	1.718GlyTrp: 1.718 ± 0.028	2.356GlyTyr: 2.356 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.878HisAla: 2.878 ± 0.038	0.224HisCys: 0.224 ± 0.01	1.387HisAsp: 1.387 ± 0.028	1.26HisGlu: 1.26 ± 0.022	0.636HisPhe: 0.636 ± 0.016	2.59HisGly: 2.59 ± 0.034	0.74HisHis: 0.74 ± 0.021	0.683HisIle: 0.683 ± 0.017	0.355HisLys: 0.355 ± 0.013	2.485HisLeu: 2.485 ± 0.038	0.332HisMet: 0.332 ± 0.01	0.356HisAsn: 0.356 ± 0.013	1.989HisPro: 1.989 ± 0.03	0.667HisGln: 0.667 ± 0.017	2.253HisArg: 2.253 ± 0.031	1.008HisSer: 1.008 ± 0.021	1.424HisThr: 1.424 ± 0.026	1.74HisVal: 1.74 ± 0.027	0.404HisTrp: 0.404 ± 0.015	0.475HisTyr: 0.475 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.551IleAla: 4.551 ± 0.047	0.269IleCys: 0.269 ± 0.012	2.037IleAsp: 2.037 ± 0.032	1.864IleGlu: 1.864 ± 0.03	0.587IlePhe: 0.587 ± 0.016	3.379IleGly: 3.379 ± 0.038	0.574IleHis: 0.574 ± 0.018	0.745IleIle: 0.745 ± 0.02	0.659IleLys: 0.659 ± 0.017	2.211IleLeu: 2.211 ± 0.031	0.394IleMet: 0.394 ± 0.012	0.624IleAsn: 0.624 ± 0.017	1.623IlePro: 1.623 ± 0.025	0.679IleGln: 0.679 ± 0.019	2.15IleArg: 2.15 ± 0.035	1.474IleSer: 1.474 ± 0.027	2.063IleThr: 2.063 ± 0.031	2.58IleVal: 2.58 ± 0.038	0.338IleTrp: 0.338 ± 0.013	0.476IleTyr: 0.476 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.782LysAla: 2.782 ± 0.049	0.111LysCys: 0.111 ± 0.007	1.296LysAsp: 1.296 ± 0.028	1.09LysGlu: 1.09 ± 0.026	0.416LysPhe: 0.416 ± 0.014	1.74LysGly: 1.74 ± 0.027	0.398LysHis: 0.398 ± 0.014	0.778LysIle: 0.778 ± 0.021	0.79LysLys: 0.79 ± 0.03	1.84LysLeu: 1.84 ± 0.027	0.351LysMet: 0.351 ± 0.013	0.474LysAsn: 0.474 ± 0.016	1.25LysPro: 1.25 ± 0.027	0.652LysGln: 0.652 ± 0.019	1.317LysArg: 1.317 ± 0.022	1.051LysSer: 1.051 ± 0.024	1.192LysThr: 1.192 ± 0.028	1.815LysVal: 1.815 ± 0.033	0.242LysTrp: 0.242 ± 0.012	0.41LysTyr: 0.41 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.83LeuAla: 15.83 ± 0.108	0.894LeuCys: 0.894 ± 0.021	6.824LeuAsp: 6.824 ± 0.058	4.627LeuGlu: 4.627 ± 0.052	2.587LeuPhe: 2.587 ± 0.042	9.463LeuGly: 9.463 ± 0.069	2.373LeuHis: 2.373 ± 0.033	3.086LeuIle: 3.086 ± 0.043	1.918LeuLys: 1.918 ± 0.036	11.715LeuLeu: 11.715 ± 0.114	1.538LeuMet: 1.538 ± 0.028	1.556LeuAsn: 1.556 ± 0.029	6.811LeuPro: 6.811 ± 0.062	2.081LeuGln: 2.081 ± 0.032	9.095LeuArg: 9.095 ± 0.069	5.08LeuSer: 5.08 ± 0.047	6.978LeuThr: 6.978 ± 0.061	9.033LeuVal: 9.033 ± 0.068	1.342LeuTrp: 1.342 ± 0.031	1.883LeuTyr: 1.883 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.222MetAla: 2.222 ± 0.029	0.134MetCys: 0.134 ± 0.007	0.842MetAsp: 0.842 ± 0.022	0.704MetGlu: 0.704 ± 0.018	0.425MetPhe: 0.425 ± 0.014	1.229MetGly: 1.229 ± 0.027	0.346MetHis: 0.346 ± 0.013	0.612MetIle: 0.612 ± 0.019	0.378MetLys: 0.378 ± 0.013	1.578MetLeu: 1.578 ± 0.03	0.277MetMet: 0.277 ± 0.011	0.406MetAsn: 0.406 ± 0.012	1.093MetPro: 1.093 ± 0.025	0.422MetGln: 0.422 ± 0.013	1.389MetArg: 1.389 ± 0.024	1.31MetSer: 1.31 ± 0.023	1.557MetThr: 1.557 ± 0.026	1.209MetVal: 1.209 ± 0.025	0.204MetTrp: 0.204 ± 0.009	0.308MetTyr: 0.308 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.085AsnAla: 2.085 ± 0.029	0.163AsnCys: 0.163 ± 0.009	0.869AsnAsp: 0.869 ± 0.019	0.723AsnGlu: 0.723 ± 0.018	0.446AsnPhe: 0.446 ± 0.014	1.795AsnGly: 1.795 ± 0.038	0.381AsnHis: 0.381 ± 0.014	0.589AsnIle: 0.589 ± 0.018	0.373AsnLys: 0.373 ± 0.015	1.566AsnLeu: 1.566 ± 0.027	0.296AsnMet: 0.296 ± 0.011	0.42AsnAsn: 0.42 ± 0.041	1.295AsnPro: 1.295 ± 0.026	0.49AsnGln: 0.49 ± 0.017	1.195AsnArg: 1.195 ± 0.022	0.884AsnSer: 0.884 ± 0.024	1.046AsnThr: 1.046 ± 0.026	1.306AsnVal: 1.306 ± 0.027	0.279AsnTrp: 0.279 ± 0.012	0.371AsnTyr: 0.371 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	9.596ProAla: 9.596 ± 0.091	0.384ProCys: 0.384 ± 0.015	4.657ProAsp: 4.657 ± 0.046	4.353ProGlu: 4.353 ± 0.05	1.558ProPhe: 1.558 ± 0.028	7.279ProGly: 7.279 ± 0.065	1.478ProHis: 1.478 ± 0.026	1.114ProIle: 1.114 ± 0.021	1.131ProLys: 1.131 ± 0.027	5.681ProLeu: 5.681 ± 0.057	0.977ProMet: 0.977 ± 0.02	0.881ProAsn: 0.881 ± 0.019	3.766ProPro: 3.766 ± 0.06	1.69ProGln: 1.69 ± 0.037	4.479ProArg: 4.479 ± 0.056	3.069ProSer: 3.069 ± 0.045	3.046ProThr: 3.046 ± 0.043	5.691ProVal: 5.691 ± 0.055	0.898ProTrp: 0.898 ± 0.022	1.477ProTyr: 1.477 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.695GlnAla: 3.695 ± 0.041	0.173GlnCys: 0.173 ± 0.009	1.385GlnAsp: 1.385 ± 0.027	1.419GlnGlu: 1.419 ± 0.026	0.63GlnPhe: 0.63 ± 0.016	2.298GlnGly: 2.298 ± 0.034	0.635GlnHis: 0.635 ± 0.02	0.983GlnIle: 0.983 ± 0.021	0.61GlnLys: 0.61 ± 0.019	2.916GlnLeu: 2.916 ± 0.037	0.467GlnMet: 0.467 ± 0.017	0.487GlnAsn: 0.487 ± 0.016	1.652GlnPro: 1.652 ± 0.035	1.17GlnGln: 1.17 ± 0.029	2.327GlnArg: 2.327 ± 0.033	1.209GlnSer: 1.209 ± 0.026	1.339GlnThr: 1.339 ± 0.026	2.287GlnVal: 2.287 ± 0.035	0.464GlnTrp: 0.464 ± 0.014	0.57GlnTyr: 0.57 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.919ArgAla: 10.919 ± 0.093	0.632ArgCys: 0.632 ± 0.019	4.313ArgAsp: 4.313 ± 0.048	4.915ArgGlu: 4.915 ± 0.063	2.346ArgPhe: 2.346 ± 0.032	6.18ArgGly: 6.18 ± 0.058	2.328ArgHis: 2.328 ± 0.036	3.114ArgIle: 3.114 ± 0.036	1.513ArgLys: 1.513 ± 0.023	9.763ArgLeu: 9.763 ± 0.079	1.766ArgMet: 1.766 ± 0.027	1.218ArgAsn: 1.218 ± 0.027	5.717ArgPro: 5.717 ± 0.07	2.34ArgGln: 2.34 ± 0.036	8.589ArgArg: 8.589 ± 0.09	3.951ArgSer: 3.951 ± 0.048	5.627ArgThr: 5.627 ± 0.046	6.172ArgVal: 6.172 ± 0.059	1.39ArgTrp: 1.39 ± 0.026	1.866ArgTyr: 1.866 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.608SerAla: 6.608 ± 0.061	0.424SerCys: 0.424 ± 0.015	2.456SerAsp: 2.456 ± 0.04	2.128SerGlu: 2.128 ± 0.037	1.467SerPhe: 1.467 ± 0.032	5.769SerGly: 5.769 ± 0.067	0.968SerHis: 0.968 ± 0.021	1.268SerIle: 1.268 ± 0.028	0.907SerLys: 0.907 ± 0.022	4.685SerLeu: 4.685 ± 0.045	1.001SerMet: 1.001 ± 0.023	0.76SerAsn: 0.76 ± 0.018	3.097SerPro: 3.097 ± 0.041	1.134SerGln: 1.134 ± 0.025	3.514SerArg: 3.514 ± 0.042	2.512SerSer: 2.512 ± 0.043	2.804SerThr: 2.804 ± 0.037	4.06SerVal: 4.06 ± 0.043	0.838SerTrp: 0.838 ± 0.02	1.182SerTyr: 1.182 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.26ThrAla: 9.26 ± 0.069	0.453ThrCys: 0.453 ± 0.016	3.578ThrAsp: 3.578 ± 0.043	3.301ThrGlu: 3.301 ± 0.042	1.55ThrPhe: 1.55 ± 0.027	7.073ThrGly: 7.073 ± 0.063	1.224ThrHis: 1.224 ± 0.02	1.564ThrIle: 1.564 ± 0.03	1.034ThrLys: 1.034 ± 0.023	5.689ThrLeu: 5.689 ± 0.049	0.886ThrMet: 0.886 ± 0.02	0.952ThrAsn: 0.952 ± 0.022	4.211ThrPro: 4.211 ± 0.04	1.282ThrGln: 1.282 ± 0.025	4.081ThrArg: 4.081 ± 0.041	2.968ThrSer: 2.968 ± 0.041	3.798ThrThr: 3.798 ± 0.055	6.059ThrVal: 6.059 ± 0.054	0.871ThrTrp: 0.871 ± 0.021	1.256ThrTyr: 1.256 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.709ValAla: 10.709 ± 0.081	0.826ValCys: 0.826 ± 0.02	4.749ValAsp: 4.749 ± 0.049	4.506ValGlu: 4.506 ± 0.052	2.394ValPhe: 2.394 ± 0.037	6.308ValGly: 6.308 ± 0.055	2.066ValHis: 2.066 ± 0.028	2.65ValIle: 2.65 ± 0.039	1.609ValLys: 1.609 ± 0.032	9.703ValLeu: 9.703 ± 0.08	1.338ValMet: 1.338 ± 0.026	1.594ValAsn: 1.594 ± 0.03	5.617ValPro: 5.617 ± 0.056	2.01ValGln: 2.01 ± 0.033	7.65ValArg: 7.65 ± 0.061	4.167ValSer: 4.167 ± 0.046	5.751ValThr: 5.751 ± 0.052	7.903ValVal: 7.903 ± 0.074	1.141ValTrp: 1.141 ± 0.022	1.623ValTyr: 1.623 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.697TrpAla: 1.697 ± 0.024	0.159TrpCys: 0.159 ± 0.008	0.809TrpAsp: 0.809 ± 0.021	0.706TrpGlu: 0.706 ± 0.019	0.493TrpPhe: 0.493 ± 0.014	1.043TrpGly: 1.043 ± 0.022	0.376TrpHis: 0.376 ± 0.013	0.504TrpIle: 0.504 ± 0.015	0.348TrpLys: 0.348 ± 0.012	1.777TrpLeu: 1.777 ± 0.032	0.262TrpMet: 0.262 ± 0.011	0.37TrpAsn: 0.37 ± 0.014	0.798TrpPro: 0.798 ± 0.019	0.62TrpGln: 0.62 ± 0.018	1.397TrpArg: 1.397 ± 0.025	0.9TrpSer: 0.9 ± 0.021	1.037TrpThr: 1.037 ± 0.021	0.971TrpVal: 0.971 ± 0.025	0.321TrpTrp: 0.321 ± 0.012	0.363TrpTyr: 0.363 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.766TyrAla: 2.766 ± 0.038	0.179TyrCys: 0.179 ± 0.008	1.578TyrAsp: 1.578 ± 0.03	1.233TyrGlu: 1.233 ± 0.024	0.652TyrPhe: 0.652 ± 0.017	2.289TyrGly: 2.289 ± 0.033	0.404TyrHis: 0.404 ± 0.014	0.454TyrIle: 0.454 ± 0.015	0.366TyrLys: 0.366 ± 0.014	2.048TyrLeu: 2.048 ± 0.034	0.248TyrMet: 0.248 ± 0.01	0.384TyrAsn: 0.384 ± 0.014	1.05TyrPro: 1.05 ± 0.026	0.616TyrGln: 0.616 ± 0.017	1.852TyrArg: 1.852 ± 0.029	0.883TyrSer: 0.883 ± 0.02	1.221TyrThr: 1.221 ± 0.024	1.64TyrVal: 1.64 ± 0.024	0.341TyrTrp: 0.341 ± 0.011	0.441TyrTyr: 0.441 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.003

Statistics based on 7251 proteins (2351009 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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