Blastococcus sp. TF02-8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367BAF6|A0A367BAF6_9ACTN Uncharacterized protein OS=Blastococcus sp. TF02-8 OX=2250574 GN=DQ237_00840 PE=4 SV=1
MM1 pKa = 7.6SNPEE5 pKa = 3.84EE6 pKa = 4.36SVEE9 pKa = 3.97PGGIADD15 pKa = 3.56VAVYY19 pKa = 10.89GDD21 pKa = 4.06YY22 pKa = 10.88AYY24 pKa = 10.98LAAWGGEE31 pKa = 4.1TCKK34 pKa = 10.93ANGIHH39 pKa = 6.2VVDD42 pKa = 4.72ISDD45 pKa = 3.38VTNPRR50 pKa = 11.84EE51 pKa = 4.02VAFVPSKK58 pKa = 10.02EE59 pKa = 3.71GSYY62 pKa = 10.06PGEE65 pKa = 4.24GMQVTHH71 pKa = 7.24ISTPSFTGDD80 pKa = 3.15VLVTNNEE87 pKa = 3.75ICKK90 pKa = 9.27DD91 pKa = 3.43TAGFGGMNIYY101 pKa = 10.75DD102 pKa = 3.86VTNPARR108 pKa = 11.84PKK110 pKa = 9.49PLAVGFGDD118 pKa = 4.34TSAGGNNQKK127 pKa = 8.47KK128 pKa = 6.42TANEE132 pKa = 3.72IHH134 pKa = 7.19SVFAWDD140 pKa = 4.58AGNKK144 pKa = 9.41AYY146 pKa = 10.57AVMVDD151 pKa = 3.4NEE153 pKa = 4.0EE154 pKa = 4.12AADD157 pKa = 3.76VDD159 pKa = 4.44IVDD162 pKa = 3.42ITNPKK167 pKa = 9.99KK168 pKa = 9.87PVLIAEE174 pKa = 4.43YY175 pKa = 10.84DD176 pKa = 3.62LATQFPQILQPEE188 pKa = 4.22QGLDD192 pKa = 3.29EE193 pKa = 4.77VFLHH197 pKa = 7.14DD198 pKa = 5.32MIVKK202 pKa = 9.24EE203 pKa = 3.93INGRR207 pKa = 11.84FIMLASYY214 pKa = 9.77WDD216 pKa = 3.45AGYY219 pKa = 10.46VQLDD223 pKa = 3.75VTDD226 pKa = 4.66PLHH229 pKa = 6.96PIYY232 pKa = 10.84LSDD235 pKa = 4.83SDD237 pKa = 4.1FTNPDD242 pKa = 3.23PEE244 pKa = 4.31AAEE247 pKa = 4.45SGLTVPPEE255 pKa = 4.46GNGHH259 pKa = 5.29QGEE262 pKa = 4.4FTLDD266 pKa = 2.96NRR268 pKa = 11.84YY269 pKa = 10.45VIGADD274 pKa = 3.37EE275 pKa = 5.1DD276 pKa = 3.97FGPYY280 pKa = 10.73ALTARR285 pKa = 11.84NVDD288 pKa = 3.61DD289 pKa = 4.32GTDD292 pKa = 3.24IEE294 pKa = 4.59ASQGSGTTPLEE305 pKa = 4.3EE306 pKa = 5.23GDD308 pKa = 4.17TITGEE313 pKa = 4.34SVFVGRR319 pKa = 11.84ACPGDD324 pKa = 3.55PAVPAGDD331 pKa = 3.66GTQIAVVEE339 pKa = 4.28RR340 pKa = 11.84GVCDD344 pKa = 3.6FTVKK348 pKa = 10.07VAAVEE353 pKa = 4.0AAGGYY358 pKa = 8.8SAILVFNRR366 pKa = 11.84TASDD370 pKa = 3.57GCNGSLGMTVEE381 pKa = 4.3GGIPTFGVAPRR392 pKa = 11.84EE393 pKa = 3.75QGFAIFDD400 pKa = 3.66VEE402 pKa = 4.16NQYY405 pKa = 11.94DD406 pKa = 3.95DD407 pKa = 4.25AACLAGDD414 pKa = 4.65GTQLAPIPVGTTGDD428 pKa = 3.45TLAFSSYY435 pKa = 10.25FDD437 pKa = 2.94GWGYY441 pKa = 10.72VHH443 pKa = 7.13LFDD446 pKa = 4.03STSMSEE452 pKa = 4.07LDD454 pKa = 3.38TYY456 pKa = 11.08AIPEE460 pKa = 3.94AHH462 pKa = 7.15DD463 pKa = 3.4PAFATGFGDD472 pKa = 4.39LSVHH476 pKa = 6.01EE477 pKa = 4.51VATSKK482 pKa = 10.95LDD484 pKa = 3.56PNLAYY489 pKa = 10.58LSYY492 pKa = 10.1YY493 pKa = 10.83AGGLRR498 pKa = 11.84VIDD501 pKa = 3.84VSSGEE506 pKa = 4.02IEE508 pKa = 4.15EE509 pKa = 4.12VGAFIDD515 pKa = 4.07QGGNNFWGVEE525 pKa = 4.12TFVQDD530 pKa = 3.28GVEE533 pKa = 4.26YY534 pKa = 10.75VALSDD539 pKa = 4.01RR540 pKa = 11.84DD541 pKa = 3.52YY542 pKa = 11.85GLYY545 pKa = 9.05ILKK548 pKa = 9.91YY549 pKa = 10.3APP551 pKa = 3.75

Molecular weight: 58.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367B721|A0A367B721_9ACTN Exodeoxyribonuclease V OS=Blastococcus sp. TF02-8 OX=2250574 GN=DQ237_00665 PE=4 SV=1
MM1 pKa = 7.32PRR3 pKa = 11.84WRR5 pKa = 11.84RR6 pKa = 11.84PGTPRR11 pKa = 11.84GRR13 pKa = 11.84RR14 pKa = 11.84TTAPRR19 pKa = 11.84RR20 pKa = 11.84CARR23 pKa = 11.84CRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84PAGRR32 pKa = 11.84SRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84PRR38 pKa = 11.84PRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84GRR44 pKa = 11.84RR45 pKa = 11.84ARR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84PRR51 pKa = 11.84RR52 pKa = 11.84GWPSPRR58 pKa = 11.84GRR60 pKa = 11.84AVARR64 pKa = 11.84RR65 pKa = 11.84AGRR68 pKa = 3.36

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3726

0

3726

1181205

31

2253

317.0

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.14 ± 0.055

0.69 ± 0.012

6.224 ± 0.032

5.727 ± 0.039

2.555 ± 0.022

9.603 ± 0.038

1.956 ± 0.019

2.887 ± 0.027

1.539 ± 0.027

10.707 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.64 ± 0.015

1.497 ± 0.02

6.1 ± 0.036

2.788 ± 0.022

8.09 ± 0.044

4.99 ± 0.025

5.965 ± 0.035

9.671 ± 0.042

1.482 ± 0.015

1.747 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski