Trypanosoma conorhini
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10134 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A422N1M0|A0A422N1M0_9TRYP ATPase (Fragment) OS=Trypanosoma conorhini OX=83891 GN=Tco025E_09051 PE=4 SV=1
DDD2 pKa = 3.99 FNTMPVCCVVSEEE15 pKa = 5.46 KK16 pKa = 10.17 ICMHHH21 pKa = 6.71 GLSPDDD27 pKa = 3.44 TSLATVNEEE36 pKa = 4.03 EEE38 pKa = 4.72 PCDDD42 pKa = 3.59 PDDD45 pKa = 4.72 KK46 pKa = 11.36 ILCDDD51 pKa = 5.31 LWADDD56 pKa = 4.62 EEE58 pKa = 4.43 DD59 pKa = 4.0 EE60 pKa = 4.07 RR61 pKa = 11.84 GFLEEE66 pKa = 3.62 DDD68 pKa = 3.15 GVSYYY73 pKa = 11.11 FGEEE77 pKa = 5.0 DD78 pKa = 3.03 VSDDD82 pKa = 4.01 LDDD85 pKa = 3.95 VDDD88 pKa = 3.08 DDD90 pKa = 4.43 VVRR93 pKa = 11.84 AHHH96 pKa = 5.47 VMEEE100 pKa = 4.06 GYYY103 pKa = 11.14 FFANRR108 pKa = 11.84 QLVTIFSAPNYYY120 pKa = 10.13 GEEE123 pKa = 4.32 DDD125 pKa = 4.05 DDD127 pKa = 3.55 AVMNVDDD134 pKa = 4.59 EE135 pKa = 5.21 KK136 pKa = 10.92 QCSFLIIPANN
Molecular weight: 16.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.719
ProMoST 4.126
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.859
Sillero 4.05
Patrickios 1.926
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|A0A422N4F2|A0A422N4F2_9TRYP Small subunit ribosomal protein S11e (Fragment) OS=Trypanosoma conorhini OX=83891 GN=Tco025E_08820 PE=3 SV=1
MM1 pKa = 7.23 KK2 pKa = 10.24 KK3 pKa = 9.95 KK4 pKa = 10.26 RR5 pKa = 11.84 RR6 pKa = 11.84 GRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 8.23 RR11 pKa = 11.84 QKK13 pKa = 10.17 AVAVHH18 pKa = 6.0 WRR20 pKa = 11.84 PHH22 pKa = 5.12 RR23 pKa = 11.84 CQRR26 pKa = 11.84 RR27 pKa = 11.84 LRR29 pKa = 11.84 PVLLAQAQLSRR40 pKa = 11.84 PSVRR44 pKa = 11.84 RR45 pKa = 11.84 LPCSNEE51 pKa = 3.89 SLRR54 pKa = 11.84 PPQTVQPRR62 pKa = 11.84 RR63 pKa = 11.84 ARR65 pKa = 11.84 RR66 pKa = 11.84 VTARR70 pKa = 11.84 RR71 pKa = 11.84 SRR73 pKa = 11.84 ALRR76 pKa = 11.84 RR77 pKa = 11.84 TRR79 pKa = 11.84 QRR81 pKa = 11.84 PLAACRR87 pKa = 11.84 LLPPRR92 pKa = 11.84 PPARR96 pKa = 11.84 RR97 pKa = 11.84 QTLPPRR103 pKa = 11.84 KK104 pKa = 8.63 MAQTPKK110 pKa = 10.36 LRR112 pKa = 11.84 RR113 pKa = 11.84 TRR115 pKa = 11.84 TAATRR120 pKa = 11.84 HH121 pKa = 6.18 PATPPRR127 pKa = 11.84 PPRR130 pKa = 11.84 LRR132 pKa = 11.84 QLGGQAPRR140 pKa = 11.84 QHH142 pKa = 5.66 ATRR145 pKa = 11.84 RR146 pKa = 11.84 RR147 pKa = 11.84 TT148 pKa = 3.23
Molecular weight: 17.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.492
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.866
ProMoST 13.364
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.427
Grimsley 12.896
Solomon 13.364
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.149
IPC_peptide 13.364
IPC2_peptide 12.354
IPC2.peptide.svr19 9.152
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10134
0
10134
4735071
93
6644
467.2
51.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.956 ± 0.033
2.005 ± 0.01
4.611 ± 0.015
6.944 ± 0.024
3.429 ± 0.015
7.003 ± 0.023
2.506 ± 0.009
3.03 ± 0.015
3.754 ± 0.019
9.999 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.165 ± 0.01
2.772 ± 0.012
5.577 ± 0.025
3.938 ± 0.016
7.92 ± 0.026
7.253 ± 0.022
5.303 ± 0.015
7.313 ± 0.017
1.19 ± 0.008
2.269 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here