Gemmatimonas phototrophica

Taxonomy: cellular organisms; Bacteria; FCB group; Gemmatimonadetes; Gemmatimonadetes; Gemmatimonadales; Gemmatimonadaceae; Gemmatimonas

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143BP46|A0A143BP46_9BACT Uncharacterized protein OS=Gemmatimonas phototrophica OX=1379270 GN=GEMMAAP_13625 PE=4 SV=1
MM1 pKa = 7.49SGLVHH6 pKa = 6.9CPYY9 pKa = 10.27CGEE12 pKa = 4.15SVEE15 pKa = 4.5ISLDD19 pKa = 3.54PGSGANQQYY28 pKa = 10.28IEE30 pKa = 4.68DD31 pKa = 4.24CQVCCRR37 pKa = 11.84PWLVSVSYY45 pKa = 11.23DD46 pKa = 3.43EE47 pKa = 6.2DD48 pKa = 3.56GTAHH52 pKa = 5.87VFVDD56 pKa = 4.44ASDD59 pKa = 4.58DD60 pKa = 3.9HH61 pKa = 8.64DD62 pKa = 6.04SDD64 pKa = 4.77DD65 pKa = 3.81

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143BPS8|A0A143BPS8_9BACT TonB-dependent receptor OS=Gemmatimonas phototrophica OX=1379270 GN=GEMMAAP_18620 PE=4 SV=1
MM1 pKa = 7.76IDD3 pKa = 3.45PRR5 pKa = 11.84AAAAGIGKK13 pKa = 9.16IAVLLSLLACASASPVPVVASAVRR37 pKa = 11.84YY38 pKa = 9.65VRR40 pKa = 11.84ADD42 pKa = 3.34TLTPALGARR51 pKa = 11.84GLLIVCATAFPRR63 pKa = 11.84DD64 pKa = 3.33TAVANVLVQGVKK76 pKa = 10.64GGDD79 pKa = 3.45TARR82 pKa = 11.84TKK84 pKa = 11.19ADD86 pKa = 2.72GCARR90 pKa = 11.84VALPAGPVALRR101 pKa = 11.84LARR104 pKa = 11.84MEE106 pKa = 4.14FARR109 pKa = 11.84ADD111 pKa = 3.3VTALVRR117 pKa = 11.84AGFADD122 pKa = 3.49SLYY125 pKa = 10.89VRR127 pKa = 11.84LEE129 pKa = 3.8RR130 pKa = 11.84HH131 pKa = 5.82GLPTPAACRR140 pKa = 11.84AAVRR144 pKa = 11.84AGRR147 pKa = 11.84PCMM150 pKa = 4.11

Molecular weight: 15.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3388

0

3388

1195143

43

2787

352.8

38.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.689 ± 0.059

0.705 ± 0.013

5.379 ± 0.028

4.999 ± 0.04

3.345 ± 0.026

8.582 ± 0.034

2.004 ± 0.021

4.079 ± 0.03

2.449 ± 0.039

10.033 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.018

2.57 ± 0.034

5.446 ± 0.027

3.48 ± 0.025

7.763 ± 0.044

5.678 ± 0.036

6.323 ± 0.032

8.523 ± 0.033

1.463 ± 0.019

2.195 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski