Leptolyngbya valderiana BDU 20041

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Leptolyngbya; Leptolyngbya valderiana

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166VR17|A0A166VR17_9CYAN Uncharacterized protein OS=Leptolyngbya valderiana BDU 20041 OX=322866 GN=AY599_15815 PE=4 SV=1
MM1 pKa = 7.21PTLLAACGLALAAAGAALAQAVPEE25 pKa = 4.13CLYY28 pKa = 11.23AVTAQGNRR36 pKa = 11.84LYY38 pKa = 10.34RR39 pKa = 11.84VAIGPEE45 pKa = 4.04GSEE48 pKa = 4.1LVGDD52 pKa = 4.65IGPIAPDD59 pKa = 3.16AGVNRR64 pKa = 11.84LTIAGPNLAYY74 pKa = 9.68TIDD77 pKa = 3.69TQNEE81 pKa = 3.88ILHH84 pKa = 6.8GIRR87 pKa = 11.84LSDD90 pKa = 3.44ARR92 pKa = 11.84VVSSVPLDD100 pKa = 3.42QPLWISSRR108 pKa = 11.84ALAVAPDD115 pKa = 3.93GVLWGALPGAQLRR128 pKa = 11.84TIDD131 pKa = 3.94PATGVTTLVGAIAGATYY148 pKa = 10.29IEE150 pKa = 4.53GMAFAPDD157 pKa = 3.36GTLYY161 pKa = 11.1AVGNVDD167 pKa = 3.58SRR169 pKa = 11.84FSRR172 pKa = 11.84KK173 pKa = 9.72LYY175 pKa = 9.82TINTTTAAATFLHH188 pKa = 6.37SLAVSDD194 pKa = 4.73IDD196 pKa = 4.07CLAWGYY202 pKa = 11.45DD203 pKa = 3.24GFLYY207 pKa = 10.66GADD210 pKa = 3.5ADD212 pKa = 4.05GRR214 pKa = 11.84EE215 pKa = 4.02ADD217 pKa = 4.62LFRR220 pKa = 11.84IDD222 pKa = 4.27PVTGAVTVPGNTGIVGVDD240 pKa = 3.17GMAAAASCSCPADD253 pKa = 3.61LDD255 pKa = 4.17GDD257 pKa = 4.36GEE259 pKa = 4.34LTLFDD264 pKa = 4.41FLAFGVLYY272 pKa = 10.76DD273 pKa = 4.77LGSPLADD280 pKa = 3.67FDD282 pKa = 4.72GDD284 pKa = 3.56GDD286 pKa = 3.91FTIFDD291 pKa = 4.03FLAFQNAFDD300 pKa = 4.57AGCPP304 pKa = 3.59

Molecular weight: 31.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166WGN1|A0A166WGN1_9CYAN Glycine cleavage system T protein OS=Leptolyngbya valderiana BDU 20041 OX=322866 GN=AY599_16370 PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 9.73EE3 pKa = 3.78VPQAARR9 pKa = 11.84RR10 pKa = 11.84PLVEE14 pKa = 3.71RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.98ARR19 pKa = 11.84VRR21 pKa = 11.84LRR23 pKa = 11.84VVDD26 pKa = 3.59VARR29 pKa = 11.84RR30 pKa = 11.84AGLLRR35 pKa = 11.84ALGLRR40 pKa = 11.84AVVVRR45 pKa = 11.84PAVRR49 pKa = 11.84ALVVLRR55 pKa = 11.84LAVEE59 pKa = 4.61RR60 pKa = 11.84VAAGLLVRR68 pKa = 11.84ALVVRR73 pKa = 11.84LAVVRR78 pKa = 11.84AVLLRR83 pKa = 11.84AGRR86 pKa = 11.84RR87 pKa = 11.84FAVLPVLRR95 pKa = 11.84AVVRR99 pKa = 11.84LAAVPVLRR107 pKa = 11.84AVVRR111 pKa = 11.84LAVVPVLRR119 pKa = 11.84AVVRR123 pKa = 11.84LAVVPVLRR131 pKa = 11.84AVVRR135 pKa = 11.84LAAVPVLRR143 pKa = 11.84AVVRR147 pKa = 11.84LAVVPVLRR155 pKa = 11.84AVVRR159 pKa = 11.84LAVVPVLRR167 pKa = 11.84AVVRR171 pKa = 11.84LAAVPVLRR179 pKa = 11.84AVVRR183 pKa = 11.84LAVVPVLRR191 pKa = 11.84AVVRR195 pKa = 11.84LAAVPVLRR203 pKa = 11.84AVVRR207 pKa = 11.84LAAGLAVLRR216 pKa = 11.84AGLLPVVARR225 pKa = 11.84LAVVPVVRR233 pKa = 11.84VVFRR237 pKa = 11.84AVLLRR242 pKa = 11.84APVRR246 pKa = 11.84PVVRR250 pKa = 11.84LAAVLLPVARR260 pKa = 11.84VRR262 pKa = 11.84RR263 pKa = 11.84DD264 pKa = 3.22GAVRR268 pKa = 11.84AAWVRR273 pKa = 11.84LAVLAVARR281 pKa = 11.84RR282 pKa = 11.84RR283 pKa = 11.84VEE285 pKa = 3.96DD286 pKa = 3.35VLFIAA291 pKa = 5.7

Molecular weight: 31.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5400

0

5400

1905942

31

5045

353.0

38.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.655 ± 0.048

0.914 ± 0.011

6.255 ± 0.034

6.528 ± 0.036

3.68 ± 0.022

7.997 ± 0.043

1.86 ± 0.017

4.956 ± 0.024

2.784 ± 0.035

10.602 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.019

2.844 ± 0.025

5.236 ± 0.024

3.893 ± 0.022

7.305 ± 0.037

5.845 ± 0.025

5.393 ± 0.023

7.418 ± 0.027

1.46 ± 0.013

2.385 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski