Hornefia porci

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; Hornefia

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2432 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9JKG1|A0A1Q9JKG1_9FIRM AAA family ATPase OS=Hornefia porci OX=2652292 GN=BHK98_11940 PE=4 SV=1
MM1 pKa = 7.64TYY3 pKa = 11.23DD4 pKa = 4.44DD5 pKa = 5.22VITMLEE11 pKa = 4.12EE12 pKa = 4.54AGLPLAYY19 pKa = 10.36DD20 pKa = 3.87HH21 pKa = 6.69FAEE24 pKa = 5.4GEE26 pKa = 4.4SPDD29 pKa = 3.72PPFLVFLYY37 pKa = 9.99PGSDD41 pKa = 2.89NMFADD46 pKa = 3.4DD47 pKa = 4.76TVFQKK52 pKa = 10.15IDD54 pKa = 3.4EE55 pKa = 4.66LNIEE59 pKa = 5.08LYY61 pKa = 10.52TDD63 pKa = 3.09IKK65 pKa = 11.22DD66 pKa = 3.97PEE68 pKa = 4.52TEE70 pKa = 4.17TQIEE74 pKa = 4.72DD75 pKa = 3.03ILIRR79 pKa = 11.84HH80 pKa = 6.54DD81 pKa = 3.91LPYY84 pKa = 10.55EE85 pKa = 4.06KK86 pKa = 10.65SEE88 pKa = 3.77VWIEE92 pKa = 4.0SEE94 pKa = 3.82KK95 pKa = 10.87LYY97 pKa = 10.94EE98 pKa = 4.19VLYY101 pKa = 8.01QTQIIGGG108 pKa = 3.81

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9JHD0|A0A1Q9JHD0_9FIRM Mobilization protein OS=Hornefia porci OX=2652292 GN=BHK98_05760 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.45KK3 pKa = 10.18KK4 pKa = 10.82LNTLRR9 pKa = 11.84HH10 pKa = 5.93IIIAYY15 pKa = 9.74AVLLAIWQLAASFSGVSSALFPSPGRR41 pKa = 11.84VLLAFRR47 pKa = 11.84EE48 pKa = 4.46LTVSGLSGSSSDD60 pKa = 3.29ATLSVHH66 pKa = 6.62IRR68 pKa = 11.84DD69 pKa = 3.49SLLRR73 pKa = 11.84FAAGYY78 pKa = 9.67LLSAAAGITAGLLLGWFPKK97 pKa = 10.51AFAYY101 pKa = 9.21VNPVIQLIRR110 pKa = 11.84PIAPVAWLPFLVLLVGIGDD129 pKa = 4.54LPAVTIIFIAGFFPILLSTASAVQNIDD156 pKa = 3.26LVYY159 pKa = 10.85LRR161 pKa = 11.84VAGNFGLSQRR171 pKa = 11.84RR172 pKa = 11.84TLTRR176 pKa = 11.84IVFPAAFPQIAGSLHH191 pKa = 6.37LALGTCWIFLVSGEE205 pKa = 4.29MVGSQTGLGFLIMDD219 pKa = 5.06AKK221 pKa = 10.83NCLRR225 pKa = 11.84ADD227 pKa = 3.69ALLAAIITIGVIGFLLDD244 pKa = 3.41QLIGLLEE251 pKa = 4.09RR252 pKa = 11.84SFISARR258 pKa = 11.84GLGPQNNKK266 pKa = 9.16RR267 pKa = 11.84RR268 pKa = 11.84SS269 pKa = 3.34

Molecular weight: 28.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2432

0

2432

794604

37

2916

326.7

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.306 ± 0.058

1.496 ± 0.023

5.987 ± 0.043

7.354 ± 0.065

3.857 ± 0.033

7.702 ± 0.047

1.709 ± 0.021

6.855 ± 0.046

6.057 ± 0.059

8.502 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.03 ± 0.029

3.836 ± 0.034

3.599 ± 0.033

2.826 ± 0.027

5.966 ± 0.06

5.831 ± 0.048

5.517 ± 0.056

6.881 ± 0.042

0.883 ± 0.017

3.805 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski