Chryseobacterium sp. Leaf180

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Chryseobacterium group; Chryseobacterium; unclassified Chryseobacterium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5QNR1|A0A0Q5QNR1_9FLAO Uncharacterized protein OS=Chryseobacterium sp. Leaf180 OX=1736289 GN=ASG01_06475 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.54GILKK6 pKa = 9.77IYY8 pKa = 10.07HH9 pKa = 6.63PEE11 pKa = 3.61EE12 pKa = 3.72TLKK15 pKa = 11.2YY16 pKa = 9.63NIKK19 pKa = 8.74NTYY22 pKa = 9.23CKK24 pKa = 10.18AVYY27 pKa = 10.47SNQQHH32 pKa = 5.37FLEE35 pKa = 4.81VEE37 pKa = 4.4VITDD41 pKa = 3.44EE42 pKa = 4.9SLDD45 pKa = 3.87HH46 pKa = 7.49VDD48 pKa = 6.01DD49 pKa = 6.88DD50 pKa = 4.44SLQYY54 pKa = 10.88NYY56 pKa = 9.76PQLAFKK62 pKa = 10.43IYY64 pKa = 10.13DD65 pKa = 3.56FPIEE69 pKa = 4.02SDD71 pKa = 3.68EE72 pKa = 4.74ISGKK76 pKa = 8.57TINVDD81 pKa = 3.4DD82 pKa = 4.56TVDD85 pKa = 3.8EE86 pKa = 5.12IYY88 pKa = 10.73TEE90 pKa = 4.32ADD92 pKa = 4.11LYY94 pKa = 11.42CDD96 pKa = 4.82DD97 pKa = 5.45DD98 pKa = 4.3AVLYY102 pKa = 10.56GSEE105 pKa = 5.39LIFSDD110 pKa = 5.54DD111 pKa = 3.59EE112 pKa = 4.32QGDD115 pKa = 3.88LQVIWKK121 pKa = 10.46GEE123 pKa = 3.53IDD125 pKa = 3.83DD126 pKa = 4.73FYY128 pKa = 11.11TRR130 pKa = 11.84SDD132 pKa = 3.38TPLPFRR138 pKa = 11.84LKK140 pKa = 10.74CSLKK144 pKa = 10.1PDD146 pKa = 4.24EE147 pKa = 4.52ILIDD151 pKa = 3.81EE152 pKa = 4.45EE153 pKa = 4.35

Molecular weight: 17.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5QJF5|A0A0Q5QJF5_9FLAO NodB homology domain-containing protein OS=Chryseobacterium sp. Leaf180 OX=1736289 GN=ASG01_08345 PE=4 SV=1
MM1 pKa = 7.49SIVHH5 pKa = 6.6KK6 pKa = 10.17IGRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 7.17TAVARR16 pKa = 11.84VYY18 pKa = 10.3VKK20 pKa = 10.5PGTGNITVNRR30 pKa = 11.84KK31 pKa = 8.29DD32 pKa = 3.26AKK34 pKa = 10.76VYY36 pKa = 10.16FSTDD40 pKa = 2.78VMVYY44 pKa = 10.08KK45 pKa = 10.68VNQPFILTEE54 pKa = 3.95TVGQYY59 pKa = 11.19DD60 pKa = 3.33VTVNVFGGGNTGQAEE75 pKa = 4.25AVRR78 pKa = 11.84LGISRR83 pKa = 11.84ALCEE87 pKa = 3.88INAEE91 pKa = 4.0YY92 pKa = 10.77RR93 pKa = 11.84LLLKK97 pKa = 10.65PEE99 pKa = 4.27GLLTRR104 pKa = 11.84DD105 pKa = 2.83ARR107 pKa = 11.84MVEE110 pKa = 4.26RR111 pKa = 11.84KK112 pKa = 9.98KK113 pKa = 10.51PGQKK117 pKa = 9.6KK118 pKa = 9.13ARR120 pKa = 11.84KK121 pKa = 8.69RR122 pKa = 11.84FQFSKK127 pKa = 10.78RR128 pKa = 3.42

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3021

0

3021

980680

55

3488

324.6

36.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.519 ± 0.043

0.746 ± 0.014

5.448 ± 0.036

6.535 ± 0.058

5.598 ± 0.033

6.292 ± 0.045

1.649 ± 0.021

7.733 ± 0.043

8.148 ± 0.061

8.924 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.022

6.217 ± 0.044

3.43 ± 0.027

3.504 ± 0.025

3.463 ± 0.031

6.669 ± 0.04

5.638 ± 0.059

6.086 ± 0.032

1.004 ± 0.015

4.011 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski