Amino acid dipepetide frequency for Chryseobacterium sp. Leaf180

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.926AlaAla: 4.926 ± 0.08	0.51AlaCys: 0.51 ± 0.026	3.778AlaAsp: 3.778 ± 0.065	4.772AlaGlu: 4.772 ± 0.086	3.339AlaPhe: 3.339 ± 0.068	4.642AlaGly: 4.642 ± 0.076	1.033AlaHis: 1.033 ± 0.035	4.481AlaIle: 4.481 ± 0.078	5.057AlaLys: 5.057 ± 0.072	5.73AlaLeu: 5.73 ± 0.093	1.606AlaMet: 1.606 ± 0.04	3.449AlaAsn: 3.449 ± 0.078	1.833AlaPro: 1.833 ± 0.052	2.558AlaGln: 2.558 ± 0.048	2.099AlaArg: 2.099 ± 0.054	4.06AlaSer: 4.06 ± 0.082	3.73AlaThr: 3.73 ± 0.093	4.748AlaVal: 4.748 ± 0.079	0.577AlaTrp: 0.577 ± 0.027	2.267AlaTyr: 2.267 ± 0.056	0.001AlaXaa: 0.001 ± 0.001
Cys	0.462CysAla: 0.462 ± 0.022	0.102CysCys: 0.102 ± 0.012	0.412CysAsp: 0.412 ± 0.023	0.49CysGlu: 0.49 ± 0.021	0.436CysPhe: 0.436 ± 0.022	0.679CysGly: 0.679 ± 0.028	0.167CysHis: 0.167 ± 0.015	0.578CysIle: 0.578 ± 0.024	0.473CysLys: 0.473 ± 0.024	0.612CysLeu: 0.612 ± 0.026	0.145CysMet: 0.145 ± 0.012	0.363CysAsn: 0.363 ± 0.022	0.32CysPro: 0.32 ± 0.019	0.203CysGln: 0.203 ± 0.014	0.251CysArg: 0.251 ± 0.018	0.563CysSer: 0.563 ± 0.028	0.434CysThr: 0.434 ± 0.024	0.447CysVal: 0.447 ± 0.021	0.053CysTrp: 0.053 ± 0.007	0.27CysTyr: 0.27 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.587AspAla: 3.587 ± 0.066	0.374AspCys: 0.374 ± 0.019	2.851AspAsp: 2.851 ± 0.07	3.995AspGlu: 3.995 ± 0.083	4.074AspPhe: 4.074 ± 0.067	3.409AspGly: 3.409 ± 0.061	1.033AspHis: 1.033 ± 0.035	4.04AspIle: 4.04 ± 0.07	4.104AspLys: 4.104 ± 0.078	5.471AspLeu: 5.471 ± 0.077	1.032AspMet: 1.032 ± 0.03	2.64AspAsn: 2.64 ± 0.054	1.679AspPro: 1.679 ± 0.041	1.823AspGln: 1.823 ± 0.044	2.16AspArg: 2.16 ± 0.051	3.132AspSer: 3.132 ± 0.057	2.26AspThr: 2.26 ± 0.049	3.446AspVal: 3.446 ± 0.061	0.705AspTrp: 0.705 ± 0.029	2.67AspTyr: 2.67 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	3.848GluAla: 3.848 ± 0.069	0.355GluCys: 0.355 ± 0.02	3.386GluAsp: 3.386 ± 0.073	4.696GluGlu: 4.696 ± 0.105	3.165GluPhe: 3.165 ± 0.057	3.328GluGly: 3.328 ± 0.063	0.984GluHis: 0.984 ± 0.037	6.109GluIle: 6.109 ± 0.101	7.098GluLys: 7.098 ± 0.124	5.271GluLeu: 5.271 ± 0.09	1.861GluMet: 1.861 ± 0.048	5.498GluAsn: 5.498 ± 0.084	1.465GluPro: 1.465 ± 0.042	2.01GluGln: 2.01 ± 0.044	2.403GluArg: 2.403 ± 0.053	3.404GluSer: 3.404 ± 0.066	3.567GluThr: 3.567 ± 0.065	3.835GluVal: 3.835 ± 0.071	0.622GluTrp: 0.622 ± 0.027	2.438GluTyr: 2.438 ± 0.047	0.001GluXaa: 0.001 ± 0.001
Phe	3.648PheAla: 3.648 ± 0.067	0.479PheCys: 0.479 ± 0.026	3.271PheAsp: 3.271 ± 0.059	3.417PheGlu: 3.417 ± 0.062	3.114PhePhe: 3.114 ± 0.07	3.871PheGly: 3.871 ± 0.066	0.912PheHis: 0.912 ± 0.031	4.017PheIle: 4.017 ± 0.082	3.863PheLys: 3.863 ± 0.065	5.282PheLeu: 5.282 ± 0.097	1.182PheMet: 1.182 ± 0.038	3.005PheAsn: 3.005 ± 0.061	2.003PhePro: 2.003 ± 0.048	1.635PheGln: 1.635 ± 0.048	1.944PheArg: 1.944 ± 0.035	4.577PheSer: 4.577 ± 0.081	3.372PheThr: 3.372 ± 0.062	3.29PheVal: 3.29 ± 0.06	0.622PheTrp: 0.622 ± 0.026	2.476PheTyr: 2.476 ± 0.053	0.001PheXaa: 0.001 ± 0.001
Gly	4.071GlyAla: 4.071 ± 0.08	0.562GlyCys: 0.562 ± 0.028	3.148GlyAsp: 3.148 ± 0.066	3.533GlyGlu: 3.533 ± 0.059	3.807GlyPhe: 3.807 ± 0.067	4.497GlyGly: 4.497 ± 0.103	0.976GlyHis: 0.976 ± 0.035	5.302GlyIle: 5.302 ± 0.07	5.518GlyLys: 5.518 ± 0.087	5.251GlyLeu: 5.251 ± 0.076	1.663GlyMet: 1.663 ± 0.046	4.187GlyAsn: 4.187 ± 0.099	1.162GlyPro: 1.162 ± 0.032	1.963GlyGln: 1.963 ± 0.05	2.118GlyArg: 2.118 ± 0.048	4.031GlySer: 4.031 ± 0.081	3.996GlyThr: 3.996 ± 0.099	3.861GlyVal: 3.861 ± 0.07	0.732GlyTrp: 0.732 ± 0.028	2.542GlyTyr: 2.542 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.956HisAla: 0.956 ± 0.032	0.164HisCys: 0.164 ± 0.013	0.879HisAsp: 0.879 ± 0.033	0.979HisGlu: 0.979 ± 0.029	1.136HisPhe: 1.136 ± 0.044	1.004HisGly: 1.004 ± 0.034	0.482HisHis: 0.482 ± 0.024	1.202HisIle: 1.202 ± 0.039	1.034HisLys: 1.034 ± 0.036	1.707HisLeu: 1.707 ± 0.052	0.294HisMet: 0.294 ± 0.017	0.768HisAsn: 0.768 ± 0.033	0.857HisPro: 0.857 ± 0.031	0.721HisGln: 0.721 ± 0.027	0.633HisArg: 0.633 ± 0.027	1.107HisSer: 1.107 ± 0.035	0.808HisThr: 0.808 ± 0.026	0.765HisVal: 0.765 ± 0.028	0.218HisTrp: 0.218 ± 0.015	0.779HisTyr: 0.779 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.295IleAla: 5.295 ± 0.075	0.746IleCys: 0.746 ± 0.028	4.457IleAsp: 4.457 ± 0.077	4.744IleGlu: 4.744 ± 0.085	4.484IlePhe: 4.484 ± 0.084	4.681IleGly: 4.681 ± 0.077	1.257IleHis: 1.257 ± 0.041	5.959IleIle: 5.959 ± 0.101	5.586IleLys: 5.586 ± 0.083	7.298IleLeu: 7.298 ± 0.107	1.461IleMet: 1.461 ± 0.043	4.456IleAsn: 4.456 ± 0.077	3.226IlePro: 3.226 ± 0.064	2.485IleGln: 2.485 ± 0.05	2.598IleArg: 2.598 ± 0.057	6.195IleSer: 6.195 ± 0.081	4.45IleThr: 4.45 ± 0.086	4.417IleVal: 4.417 ± 0.08	0.67IleTrp: 0.67 ± 0.026	2.854IleTyr: 2.854 ± 0.062	0.005IleXaa: 0.005 ± 0.002
Lys	4.672LysAla: 4.672 ± 0.081	0.366LysCys: 0.366 ± 0.02	4.813LysAsp: 4.813 ± 0.088	5.624LysGlu: 5.624 ± 0.093	3.635LysPhe: 3.635 ± 0.063	4.205LysGly: 4.205 ± 0.069	1.149LysHis: 1.149 ± 0.038	7.673LysIle: 7.673 ± 0.093	7.537LysLys: 7.537 ± 0.115	6.401LysLeu: 6.401 ± 0.093	2.515LysMet: 2.515 ± 0.055	6.571LysAsn: 6.571 ± 0.099	2.651LysPro: 2.651 ± 0.058	2.436LysGln: 2.436 ± 0.059	2.549LysArg: 2.549 ± 0.052	4.96LysSer: 4.96 ± 0.079	5.119LysThr: 5.119 ± 0.087	4.623LysVal: 4.623 ± 0.073	0.725LysTrp: 0.725 ± 0.036	3.255LysTyr: 3.255 ± 0.06	0.003LysXaa: 0.003 ± 0.002
Leu	5.825LeuAla: 5.825 ± 0.082	0.681LeuCys: 0.681 ± 0.031	4.659LeuAsp: 4.659 ± 0.076	5.464LeuGlu: 5.464 ± 0.095	4.554LeuPhe: 4.554 ± 0.081	5.53LeuGly: 5.53 ± 0.086	1.548LeuHis: 1.548 ± 0.042	6.639LeuIle: 6.639 ± 0.097	7.972LeuLys: 7.972 ± 0.107	7.979LeuLeu: 7.979 ± 0.115	2.241LeuMet: 2.241 ± 0.05	5.625LeuAsn: 5.625 ± 0.084	3.658LeuPro: 3.658 ± 0.071	3.448LeuGln: 3.448 ± 0.059	3.127LeuArg: 3.127 ± 0.055	6.629LeuSer: 6.629 ± 0.086	4.984LeuThr: 4.984 ± 0.068	4.772LeuVal: 4.772 ± 0.075	0.788LeuTrp: 0.788 ± 0.026	3.117LeuTyr: 3.117 ± 0.063	0.001LeuXaa: 0.001 ± 0.001
Met	1.539MetAla: 1.539 ± 0.041	0.137MetCys: 0.137 ± 0.011	1.092MetAsp: 1.092 ± 0.028	1.444MetGlu: 1.444 ± 0.04	0.944MetPhe: 0.944 ± 0.035	1.351MetGly: 1.351 ± 0.038	0.378MetHis: 0.378 ± 0.02	1.924MetIle: 1.924 ± 0.042	2.696MetLys: 2.696 ± 0.058	2.019MetLeu: 2.019 ± 0.046	0.827MetMet: 0.827 ± 0.035	1.499MetAsn: 1.499 ± 0.035	0.934MetPro: 0.934 ± 0.032	0.913MetGln: 0.913 ± 0.032	0.942MetArg: 0.942 ± 0.031	1.529MetSer: 1.529 ± 0.038	1.325MetThr: 1.325 ± 0.036	1.371MetVal: 1.371 ± 0.043	0.173MetTrp: 0.173 ± 0.014	0.781MetTyr: 0.781 ± 0.029	0.001MetXaa: 0.001 ± 0.001
Asn	4.076AsnAla: 4.076 ± 0.083	0.441AsnCys: 0.441 ± 0.021	3.103AsnAsp: 3.103 ± 0.058	3.541AsnGlu: 3.541 ± 0.059	3.679AsnPhe: 3.679 ± 0.068	4.208AsnGly: 4.208 ± 0.089	1.055AsnHis: 1.055 ± 0.038	5.125AsnIle: 5.125 ± 0.085	3.934AsnLys: 3.934 ± 0.068	5.961AsnLeu: 5.961 ± 0.091	1.272AsnMet: 1.272 ± 0.036	3.624AsnAsn: 3.624 ± 0.088	2.927AsnPro: 2.927 ± 0.058	2.252AsnGln: 2.252 ± 0.053	2.23AsnArg: 2.23 ± 0.051	4.108AsnSer: 4.108 ± 0.08	3.318AsnThr: 3.318 ± 0.081	3.737AsnVal: 3.737 ± 0.069	0.765AsnTrp: 0.765 ± 0.03	2.816AsnTyr: 2.816 ± 0.066	0.0AsnXaa: 0.0 ± 0.0
Pro	2.423ProAla: 2.423 ± 0.06	0.232ProCys: 0.232 ± 0.016	1.96ProAsp: 1.96 ± 0.056	3.048ProGlu: 3.048 ± 0.06	1.982ProPhe: 1.982 ± 0.042	1.987ProGly: 1.987 ± 0.046	0.555ProHis: 0.555 ± 0.026	2.186ProIle: 2.186 ± 0.05	2.556ProLys: 2.556 ± 0.054	2.816ProLeu: 2.816 ± 0.056	0.794ProMet: 0.794 ± 0.028	2.169ProAsn: 2.169 ± 0.057	0.863ProPro: 0.863 ± 0.037	1.344ProGln: 1.344 ± 0.042	0.917ProArg: 0.917 ± 0.028	2.109ProSer: 2.109 ± 0.047	2.016ProThr: 2.016 ± 0.057	2.709ProVal: 2.709 ± 0.055	0.295ProTrp: 0.295 ± 0.019	1.342ProTyr: 1.342 ± 0.036	0.001ProXaa: 0.001 ± 0.001
Gln	1.85GlnAla: 1.85 ± 0.044	0.165GlnCys: 0.165 ± 0.012	1.643GlnAsp: 1.643 ± 0.041	2.187GlnGlu: 2.187 ± 0.044	1.739GlnPhe: 1.739 ± 0.038	1.642GlnGly: 1.642 ± 0.045	0.57GlnHis: 0.57 ± 0.027	3.01GlnIle: 3.01 ± 0.053	3.501GlnLys: 3.501 ± 0.071	3.174GlnLeu: 3.174 ± 0.067	0.965GlnMet: 0.965 ± 0.031	2.901GlnAsn: 2.901 ± 0.064	1.149GlnPro: 1.149 ± 0.035	1.53GlnGln: 1.53 ± 0.041	1.277GlnArg: 1.277 ± 0.043	2.102GlnSer: 2.102 ± 0.043	2.062GlnThr: 2.062 ± 0.054	1.785GlnVal: 1.785 ± 0.045	0.34GlnTrp: 0.34 ± 0.022	1.446GlnTyr: 1.446 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	1.903ArgAla: 1.903 ± 0.051	0.201ArgCys: 0.201 ± 0.015	1.858ArgAsp: 1.858 ± 0.04	2.445ArgGlu: 2.445 ± 0.055	2.069ArgPhe: 2.069 ± 0.046	1.804ArgGly: 1.804 ± 0.053	0.558ArgHis: 0.558 ± 0.026	2.85ArgIle: 2.85 ± 0.052	3.243ArgLys: 3.243 ± 0.065	3.079ArgLeu: 3.079 ± 0.06	0.983ArgMet: 0.983 ± 0.031	2.48ArgAsn: 2.48 ± 0.055	1.088ArgPro: 1.088 ± 0.038	1.21ArgGln: 1.21 ± 0.04	1.368ArgArg: 1.368 ± 0.043	1.984ArgSer: 1.984 ± 0.046	1.771ArgThr: 1.771 ± 0.049	1.914ArgVal: 1.914 ± 0.049	0.369ArgTrp: 0.369 ± 0.019	1.453ArgTyr: 1.453 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	4.749SerAla: 4.749 ± 0.081	0.613SerCys: 0.613 ± 0.027	3.709SerAsp: 3.709 ± 0.059	4.588SerGlu: 4.588 ± 0.059	3.967SerPhe: 3.967 ± 0.075	5.082SerGly: 5.082 ± 0.103	1.081SerHis: 1.081 ± 0.035	4.471SerIle: 4.471 ± 0.064	4.974SerLys: 4.974 ± 0.073	5.918SerLeu: 5.918 ± 0.089	1.398SerMet: 1.398 ± 0.043	3.382SerAsn: 3.382 ± 0.075	2.234SerPro: 2.234 ± 0.051	2.478SerGln: 2.478 ± 0.055	2.12SerArg: 2.12 ± 0.055	4.342SerSer: 4.342 ± 0.088	3.595SerThr: 3.595 ± 0.079	4.641SerVal: 4.641 ± 0.069	0.695SerTrp: 0.695 ± 0.03	2.649SerTyr: 2.649 ± 0.059	0.001SerXaa: 0.001 ± 0.001
Thr	4.326ThrAla: 4.326 ± 0.099	0.346ThrCys: 0.346 ± 0.02	3.303ThrAsp: 3.303 ± 0.065	3.738ThrGlu: 3.738 ± 0.067	3.075ThrPhe: 3.075 ± 0.061	4.121ThrGly: 4.121 ± 0.081	0.859ThrHis: 0.859 ± 0.034	3.738ThrIle: 3.738 ± 0.067	3.737ThrLys: 3.737 ± 0.061	4.994ThrLeu: 4.994 ± 0.082	1.062ThrMet: 1.062 ± 0.034	2.941ThrAsn: 2.941 ± 0.081	2.365ThrPro: 2.365 ± 0.062	1.909ThrGln: 1.909 ± 0.053	1.623ThrArg: 1.623 ± 0.042	3.741ThrSer: 3.741 ± 0.084	3.417ThrThr: 3.417 ± 0.108	4.343ThrVal: 4.343 ± 0.084	0.519ThrTrp: 0.519 ± 0.026	2.225ThrTyr: 2.225 ± 0.066	0.001ThrXaa: 0.001 ± 0.001
Val	4.122ValAla: 4.122 ± 0.073	0.542ValCys: 0.542 ± 0.024	3.127ValAsp: 3.127 ± 0.06	3.734ValGlu: 3.734 ± 0.066	3.492ValPhe: 3.492 ± 0.065	3.769ValGly: 3.769 ± 0.065	0.918ValHis: 0.918 ± 0.034	4.659ValIle: 4.659 ± 0.064	4.933ValLys: 4.933 ± 0.072	5.533ValLeu: 5.533 ± 0.084	1.469ValMet: 1.469 ± 0.043	3.594ValAsn: 3.594 ± 0.072	2.205ValPro: 2.205 ± 0.052	1.992ValGln: 1.992 ± 0.047	2.07ValArg: 2.07 ± 0.048	4.55ValSer: 4.55 ± 0.077	3.43ValThr: 3.43 ± 0.081	3.636ValVal: 3.636 ± 0.071	0.616ValTrp: 0.616 ± 0.029	2.471ValTyr: 2.471 ± 0.049	0.001ValXaa: 0.001 ± 0.001
Trp	0.601TrpAla: 0.601 ± 0.026	0.083TrpCys: 0.083 ± 0.01	0.559TrpAsp: 0.559 ± 0.029	0.643TrpGlu: 0.643 ± 0.028	0.579TrpPhe: 0.579 ± 0.027	0.625TrpGly: 0.625 ± 0.029	0.196TrpHis: 0.196 ± 0.018	0.711TrpIle: 0.711 ± 0.029	0.968TrpLys: 0.968 ± 0.032	0.91TrpLeu: 0.91 ± 0.033	0.31TrpMet: 0.31 ± 0.016	0.713TrpAsn: 0.713 ± 0.025	0.173TrpPro: 0.173 ± 0.015	0.435TrpGln: 0.435 ± 0.022	0.366TrpArg: 0.366 ± 0.019	0.61TrpSer: 0.61 ± 0.025	0.511TrpThr: 0.511 ± 0.022	0.528TrpVal: 0.528 ± 0.028	0.125TrpTrp: 0.125 ± 0.012	0.399TrpTyr: 0.399 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.317TyrAla: 2.317 ± 0.054	0.362TyrCys: 0.362 ± 0.02	2.474TyrAsp: 2.474 ± 0.067	2.263TyrGlu: 2.263 ± 0.048	2.709TyrPhe: 2.709 ± 0.055	2.482TyrGly: 2.482 ± 0.053	0.783TyrHis: 0.783 ± 0.028	2.649TyrIle: 2.649 ± 0.061	2.699TyrLys: 2.699 ± 0.052	3.83TyrLeu: 3.83 ± 0.059	0.739TyrMet: 0.739 ± 0.03	2.306TyrAsn: 2.306 ± 0.064	1.512TyrPro: 1.512 ± 0.035	1.69TyrGln: 1.69 ± 0.048	1.835TyrArg: 1.835 ± 0.043	2.955TyrSer: 2.955 ± 0.058	2.217TyrThr: 2.217 ± 0.056	1.995TyrVal: 1.995 ± 0.044	0.434TyrTrp: 0.434 ± 0.024	1.862TyrTyr: 1.862 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.004XaaLeu: 0.004 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3021 proteins (980680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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