Proteome-pI 2.0 - Proteome Isoelectric Point Database

Gordonia hirsuta DSM 44140 = NBRC 16056

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia hirsuta

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|L7LBA0|L7LBA0_9ACTN Putative acyl-CoA dehydrogenase OS=Gordonia hirsuta DSM 44140 = NBRC 16056 OX=1121927 GN=GOHSU_28_00410 PE=3 SV=1

MM1 pKa = 7.18AQALRR6 pKa = 11.84LVLSADD12 pKa = 3.74PDD14 pKa = 4.2DD15 pKa = 6.38DD16 pKa = 4.01PVQTGGEE23 pKa = 3.99VDD25 pKa = 3.85EE26 pKa = 4.89EE27 pKa = 4.05RR28 pKa = 11.84LLQIMAIAHH37 pKa = 5.47SAAADD42 pKa = 3.61GHH44 pKa = 6.02PLPLEE49 pKa = 4.1EE50 pKa = 5.6RR51 pKa = 11.84DD52 pKa = 4.04DD53 pKa = 4.17VSVDD57 pKa = 3.75LGPGATVSAFSDD69 pKa = 3.76SAPPEE74 pKa = 4.22CPRR77 pKa = 11.84CHH79 pKa = 6.56EE80 pKa = 5.59LLDD83 pKa = 3.83EE84 pKa = 4.88WDD86 pKa = 4.07PQPWWDD92 pKa = 4.39GGDD95 pKa = 3.74EE96 pKa = 4.14PTEE99 pKa = 3.99EE100 pKa = 4.63CPNCGFTALIGDD112 pKa = 3.99WDD114 pKa = 3.94ITNTPYY120 pKa = 11.31ARR122 pKa = 11.84TEE124 pKa = 4.08CGLVLVDD131 pKa = 4.45WPDD134 pKa = 3.37LPEE137 pKa = 4.45YY138 pKa = 11.06GPGLHH143 pKa = 6.54EE144 pKa = 4.96ALLAAVGSRR153 pKa = 11.84PRR155 pKa = 11.84YY156 pKa = 9.27VSGNTT161 pKa = 3.21

Molecular weight: 17.35 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|L7LAM9|L7LAM9_9ACTN Uncharacterized protein OS=Gordonia hirsuta DSM 44140 = NBRC 16056 OX=1121927 GN=GOHSU_16_00740 PE=4 SV=1

MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84SIVTSRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.69GRR42 pKa = 11.84AKK44 pKa = 9.63LTAA47 pKa = 4.21

Molecular weight: 5.56 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3215	0	3215	1071027	43	11828	333.1	35.63

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
13.281 ± 0.061	0.686 ± 0.011	6.407 ± 0.036	5.377 ± 0.041	2.921 ± 0.024	9.114 ± 0.037	1.973 ± 0.018	4.362 ± 0.03	2.106 ± 0.029	10.004 ± 0.046

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.01 ± 0.018	2.024 ± 0.022	5.843 ± 0.033	3.146 ± 0.026	7.223 ± 0.041	5.215 ± 0.028	6.151 ± 0.033	8.686 ± 0.049	1.383 ± 0.016	2.087 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski