candidate division MSBL1 archaeon SCGC-AAA261G05
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 601 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A133VC90|A0A133VC90_9EURY ATP-grasp domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA261G05 OX=1698276 GN=AKJ47_00925 PE=4 SV=1
MM1 pKa = 7.77 FDD3 pKa = 3.55 QNTLSSRR10 pKa = 11.84 SILIVLISIFVLSTLLPGQAHH31 pKa = 6.51 AADD34 pKa = 4.36 DD35 pKa = 3.99 NGEE38 pKa = 4.12 EE39 pKa = 4.57 EE40 pKa = 5.01 GPFWGQPWWRR50 pKa = 11.84 IGLSLGASMFAMVCISYY67 pKa = 8.36 ATIDD71 pKa = 4.27 LGIEE75 pKa = 3.87 NDD77 pKa = 4.05 GFWIVNFLALLLILMGVFTAGINGANPFFWLSIIMALCLFAVIFSALIVMYY128 pKa = 9.11 FSQRR132 pKa = 11.84 II133 pKa = 3.48
Molecular weight: 14.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 4.177
IPC_protein 4.012
Toseland 3.834
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.859
Grimsley 3.757
Solomon 3.973
Lehninger 3.935
Nozaki 4.139
DTASelect 4.317
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.139
Patrickios 1.952
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.027
Protein with the highest isoelectric point:
>tr|A0A133VC63|A0A133VC63_9EURY ABC transmembrane type-1 domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA261G05 OX=1698276 GN=AKJ47_01010 PE=3 SV=1
MM1 pKa = 7.87 PATKK5 pKa = 9.99 RR6 pKa = 11.84 CSFCDD11 pKa = 3.36 EE12 pKa = 4.09 RR13 pKa = 11.84 VTPGRR18 pKa = 11.84 GIMYY22 pKa = 8.44 VKK24 pKa = 10.26 RR25 pKa = 11.84 DD26 pKa = 3.24 GRR28 pKa = 11.84 IQYY31 pKa = 9.3 YY32 pKa = 10.24 CSSKK36 pKa = 10.16 CRR38 pKa = 11.84 RR39 pKa = 11.84 NAEE42 pKa = 4.38 LGRR45 pKa = 11.84 KK46 pKa = 5.22 PHH48 pKa = 5.68 KK49 pKa = 10.73 VKK51 pKa = 8.19 WTKK54 pKa = 10.24 RR55 pKa = 11.84 SRR57 pKa = 11.84 EE58 pKa = 4.02 ARR60 pKa = 11.84 GKK62 pKa = 9.54 EE63 pKa = 3.81
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.648
IPC_protein 10.131
Toseland 10.628
ProMoST 10.233
Dawson 10.73
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 11.082
Grimsley 10.774
Solomon 10.804
Lehninger 10.789
Nozaki 10.643
DTASelect 10.379
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.833
IPC_peptide 10.818
IPC2_peptide 9.516
IPC2.peptide.svr19 8.534
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
601
0
601
140086
50
1136
233.1
26.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.879 ± 0.116
0.991 ± 0.038
5.437 ± 0.088
9.255 ± 0.13
3.733 ± 0.088
7.766 ± 0.089
1.798 ± 0.041
7.056 ± 0.085
7.258 ± 0.102
9.755 ± 0.135
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.182 ± 0.044
3.267 ± 0.063
4.216 ± 0.054
2.387 ± 0.052
5.96 ± 0.099
6.095 ± 0.077
4.901 ± 0.069
7.18 ± 0.079
1.069 ± 0.04
2.815 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here