Notoacmeibacter marinus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Notoacmeibacteraceae; Notoacmeibacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3077 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A231V1A4|A0A231V1A4_9RHIZ Thiamine biosynthesis protein ThiF OS=Notoacmeibacter marinus OX=1876515 GN=B7H23_02390 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 10.25ILLATTAFALMATPVMAADD21 pKa = 5.31AIDD24 pKa = 3.9YY25 pKa = 7.72SAPTPAPAYY34 pKa = 9.95DD35 pKa = 3.56VAPEE39 pKa = 3.94RR40 pKa = 11.84FGWDD44 pKa = 2.56GGYY47 pKa = 10.87AGAQVGYY54 pKa = 9.89GFAEE58 pKa = 4.8ADD60 pKa = 3.88PGDD63 pKa = 4.56DD64 pKa = 3.23PSGIVGGVHH73 pKa = 6.97AGYY76 pKa = 11.0LMQYY80 pKa = 9.97GSFVVGPEE88 pKa = 3.72FDD90 pKa = 3.54VDD92 pKa = 3.32ASGIDD97 pKa = 4.52GEE99 pKa = 5.13DD100 pKa = 3.43LDD102 pKa = 4.6IDD104 pKa = 4.16VIARR108 pKa = 11.84AKK110 pKa = 10.38LKK112 pKa = 10.94AGVAVDD118 pKa = 3.85RR119 pKa = 11.84VLISGTVGYY128 pKa = 10.42AHH130 pKa = 7.6AWGDD134 pKa = 3.53SDD136 pKa = 4.25TDD138 pKa = 3.93GDD140 pKa = 4.09VSDD143 pKa = 4.59GALEE147 pKa = 4.16VGAGLDD153 pKa = 4.25FAATDD158 pKa = 4.08NIVVGSDD165 pKa = 3.32YY166 pKa = 10.18MYY168 pKa = 11.01HH169 pKa = 6.51NFGEE173 pKa = 4.41FDD175 pKa = 3.68DD176 pKa = 4.41TDD178 pKa = 5.18ADD180 pKa = 3.81VDD182 pKa = 3.99LHH184 pKa = 5.05TVRR187 pKa = 11.84ARR189 pKa = 11.84VSYY192 pKa = 10.81KK193 pKa = 10.23FF194 pKa = 3.39

Molecular weight: 20.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A231V0S5|A0A231V0S5_9RHIZ Uncharacterized protein OS=Notoacmeibacter marinus OX=1876515 GN=B7H23_02360 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.42AGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3077

0

3077

985364

35

2830

320.2

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.937 ± 0.048

0.848 ± 0.013

6.347 ± 0.036

6.301 ± 0.04

3.784 ± 0.027

8.494 ± 0.043

2.033 ± 0.022

5.472 ± 0.028

3.271 ± 0.034

9.829 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.657 ± 0.02

2.635 ± 0.021

4.913 ± 0.035

3.032 ± 0.022

7.167 ± 0.051

5.522 ± 0.034

5.308 ± 0.026

7.041 ± 0.031

1.258 ± 0.018

2.152 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski