Bacteroides sp. OM08-11
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3094 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A374VCW7|A0A374VCW7_9BACE 30S ribosomal protein S19 OS=Bacteroides sp. OM08-11 OX=2292284 GN=rpsS PE=3 SV=1
MM1 pKa = 7.32 GLEE4 pKa = 5.16 DD5 pKa = 5.85 DD6 pKa = 4.35 FLKK9 pKa = 11.18 NDD11 pKa = 3.22 VDD13 pKa = 3.99 DD14 pKa = 4.93 EE15 pKa = 4.36 KK16 pKa = 10.64 TIEE19 pKa = 4.15 YY20 pKa = 9.37 IKK22 pKa = 10.96 NYY24 pKa = 9.95 LPQEE28 pKa = 4.16 LKK30 pKa = 10.91 EE31 pKa = 4.3 KK32 pKa = 10.78 FSDD35 pKa = 3.62 DD36 pKa = 3.07 EE37 pKa = 4.59 FYY39 pKa = 11.59 YY40 pKa = 10.87 FLDD43 pKa = 5.8 LIDD46 pKa = 4.79 EE47 pKa = 4.72 YY48 pKa = 11.42 YY49 pKa = 8.9 STSGILDD56 pKa = 3.56 VQPDD60 pKa = 3.72 AEE62 pKa = 4.69 GYY64 pKa = 10.27 IDD66 pKa = 4.35 IDD68 pKa = 3.4 LGKK71 pKa = 10.23 IVDD74 pKa = 4.12 YY75 pKa = 10.58 IIKK78 pKa = 9.45 EE79 pKa = 3.91 AHH81 pKa = 6.54 KK82 pKa = 10.95 DD83 pKa = 3.51 EE84 pKa = 5.7 IGDD87 pKa = 3.88 YY88 pKa = 10.09 EE89 pKa = 4.18 PEE91 pKa = 4.45 EE92 pKa = 3.8 ILFIVQGEE100 pKa = 4.27 MEE102 pKa = 4.35 YY103 pKa = 10.46 TEE105 pKa = 4.41 SLEE108 pKa = 5.06 DD109 pKa = 3.48 EE110 pKa = 4.5
Molecular weight: 13.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.452
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.834
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.567
Sillero 3.77
Patrickios 0.985
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A374VAJ7|A0A374VAJ7_9BACE Dolichyl-phosphate-mannose--protein mannosyltransferase OS=Bacteroides sp. OM08-11 OX=2292284 GN=DXC10_07580 PE=4 SV=1
MM1 pKa = 7.3 LHH3 pKa = 6.72 LSKK6 pKa = 10.75 HH7 pKa = 5.24 RR8 pKa = 11.84 LGVRR12 pKa = 11.84 QCPFLQLCGSRR23 pKa = 11.84 HH24 pKa = 4.3 TFVPNSFWAMVCRR37 pKa = 11.84 RR38 pKa = 11.84 TIPLLRR44 pKa = 11.84 KK45 pKa = 9.14 ILMNEE50 pKa = 4.52 GIKK53 pKa = 10.74 NGSLYY58 pKa = 10.88 QNLEE62 pKa = 4.19 LNSYY66 pKa = 8.75 FCIRR70 pKa = 11.84 RR71 pKa = 11.84 VLL73 pKa = 3.48
Molecular weight: 8.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.692
IPC_protein 10.584
Toseland 10.643
ProMoST 10.394
Dawson 10.76
Bjellqvist 10.526
Wikipedia 10.994
Rodwell 10.906
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.716
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.984
IPC2.peptide.svr19 8.392
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3094
0
3094
1151197
24
1938
372.1
42.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.898 ± 0.04
1.253 ± 0.015
5.475 ± 0.031
6.571 ± 0.034
4.559 ± 0.028
6.863 ± 0.038
1.891 ± 0.019
7.1 ± 0.04
6.72 ± 0.036
9.12 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.773 ± 0.02
5.198 ± 0.034
3.699 ± 0.022
3.407 ± 0.021
4.596 ± 0.031
5.936 ± 0.029
5.685 ± 0.028
6.505 ± 0.032
1.271 ± 0.018
4.482 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here