Streptococcus satellite phage Javan339
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKE2|A0A4D5ZKE2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan339 OX=2558648 GN=JavanS339_0004 PE=4 SV=1
MM1 pKa = 7.79 EE2 pKa = 4.76 EE3 pKa = 4.01 HH4 pKa = 5.62 QQAHH8 pKa = 6.72 PEE10 pKa = 3.91 YY11 pKa = 9.84 EE12 pKa = 4.05 RR13 pKa = 11.84 YY14 pKa = 8.55 NFKK17 pKa = 10.94 YY18 pKa = 10.39 IEE20 pKa = 4.96 DD21 pKa = 3.85 GDD23 pKa = 4.63 AIGAIIDD30 pKa = 3.91 YY31 pKa = 10.28 NVEE34 pKa = 3.9 EE35 pKa = 4.49 SVLKK39 pKa = 10.84 AEE41 pKa = 5.0 KK42 pKa = 10.35 EE43 pKa = 3.79 QDD45 pKa = 3.3 NAA47 pKa = 4.02
Molecular weight: 5.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.212
IPC2_protein 4.329
IPC_protein 4.177
Toseland 4.037
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 3.986
Rodwell 4.024
Grimsley 3.948
Solomon 4.113
Lehninger 4.062
Nozaki 4.24
DTASelect 4.342
Thurlkill 4.05
EMBOSS 4.012
Sillero 4.291
Patrickios 3.083
IPC_peptide 4.113
IPC2_peptide 4.279
IPC2.peptide.svr19 4.19
Protein with the highest isoelectric point:
>tr|A0A4D5ZKW2|A0A4D5ZKW2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan339 OX=2558648 GN=JavanS339_0012 PE=4 SV=1
MM1 pKa = 7.81 KK2 pKa = 9.96 FQQGEE7 pKa = 4.43 VYY9 pKa = 10.72 LINFPQKK16 pKa = 10.5 GGNEE20 pKa = 4.58 FYY22 pKa = 10.56 GKK24 pKa = 9.64 HH25 pKa = 4.67 YY26 pKa = 10.66 AIILTIPDD34 pKa = 3.89 KK35 pKa = 11.42 ADD37 pKa = 3.15 GTLLVAPLTGKK48 pKa = 10.64 KK49 pKa = 9.69 SGKK52 pKa = 9.46 KK53 pKa = 8.13 YY54 pKa = 10.33 RR55 pKa = 11.84 GGITIEE61 pKa = 3.65 NSKK64 pKa = 9.7 YY65 pKa = 10.31 QNTPSKK71 pKa = 9.27 PKK73 pKa = 9.77 AYY75 pKa = 10.12 AYY77 pKa = 9.91 VRR79 pKa = 11.84 KK80 pKa = 9.47 IQEE83 pKa = 3.23 IDD85 pKa = 3.01 KK86 pKa = 10.87 RR87 pKa = 11.84 KK88 pKa = 9.65 IVYY91 pKa = 7.82 KK92 pKa = 9.5 TKK94 pKa = 10.76 KK95 pKa = 8.95 KK96 pKa = 9.06 TDD98 pKa = 3.08 SDD100 pKa = 4.19 GQVMLDD106 pKa = 3.32 AAGKK110 pKa = 8.79 EE111 pKa = 4.41 LYY113 pKa = 10.61 DD114 pKa = 3.53 KK115 pKa = 10.85 VYY117 pKa = 10.73 RR118 pKa = 11.84 PAYY121 pKa = 9.72 KK122 pKa = 10.41 LDD124 pKa = 3.84 TNDD127 pKa = 4.16 HH128 pKa = 6.5 KK129 pKa = 11.5 KK130 pKa = 10.69 LLDD133 pKa = 4.17 KK134 pKa = 10.82 IKK136 pKa = 10.64 EE137 pKa = 4.04 VLGLDD142 pKa = 4.25 LYY144 pKa = 11.51
Molecular weight: 16.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.072
IPC2_protein 9.121
IPC_protein 8.975
Toseland 9.75
ProMoST 9.458
Dawson 9.984
Bjellqvist 9.619
Wikipedia 10.145
Rodwell 10.467
Grimsley 10.058
Solomon 9.999
Lehninger 9.955
Nozaki 9.706
DTASelect 9.633
Thurlkill 9.809
EMBOSS 10.16
Sillero 9.882
Patrickios 7.585
IPC_peptide 9.999
IPC2_peptide 8.039
IPC2.peptide.svr19 8.221
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
3338
44
484
159.0
18.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.111 ± 0.581
0.449 ± 0.144
6.681 ± 0.516
8.957 ± 0.687
3.865 ± 0.35
4.733 ± 0.339
1.738 ± 0.158
6.321 ± 0.537
9.946 ± 0.649
9.706 ± 0.575
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.037 ± 0.242
6.141 ± 0.353
2.487 ± 0.201
3.625 ± 0.361
4.434 ± 0.329
5.003 ± 0.395
5.872 ± 0.449
5.992 ± 0.481
1.138 ± 0.136
4.763 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here