Capybara microvirus Cap1_SP_141
Average proteome isoelectric point is 5.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5E7|A0A4P8W5E7_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_141 OX=2585387 PE=4 SV=1
MM1 pKa = 7.52 IKK3 pKa = 10.17 SIYY6 pKa = 9.48 CIRR9 pKa = 11.84 DD10 pKa = 3.23 VAIGYY15 pKa = 8.38 CDD17 pKa = 4.02 PFCALNDD24 pKa = 3.57 KK25 pKa = 10.61 VAIRR29 pKa = 11.84 DD30 pKa = 3.45 FRR32 pKa = 11.84 NAINLPNTPYY42 pKa = 11.17 NLNPKK47 pKa = 10.02 DD48 pKa = 3.93 YY49 pKa = 11.32 DD50 pKa = 4.05 LMCVASIDD58 pKa = 4.15 LDD60 pKa = 3.79 SGTVVAQQPQLVVSGISLVGVNNDD84 pKa = 2.97
Molecular weight: 9.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.011
IPC2_protein 4.291
IPC_protein 4.177
Toseland 3.935
ProMoST 4.24
Dawson 4.215
Bjellqvist 4.469
Wikipedia 4.24
Rodwell 4.012
Grimsley 3.846
Solomon 4.202
Lehninger 4.164
Nozaki 4.355
DTASelect 4.711
Thurlkill 4.037
EMBOSS 4.24
Sillero 4.317
Patrickios 0.846
IPC_peptide 4.19
IPC2_peptide 4.279
IPC2.peptide.svr19 4.259
Protein with the highest isoelectric point:
>tr|A0A4P8W7Y7|A0A4P8W7Y7_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_141 OX=2585387 PE=4 SV=1
MM1 pKa = 7.07 CTSPKK6 pKa = 10.24 KK7 pKa = 10.34 AFCVGLTKK15 pKa = 10.51 NGKK18 pKa = 7.75 PDD20 pKa = 3.69 YY21 pKa = 10.98 VIAPYY26 pKa = 9.9 QCHH29 pKa = 5.48 HH30 pKa = 6.1 VEE32 pKa = 4.25 KK33 pKa = 10.51 IGDD36 pKa = 3.59 KK37 pKa = 10.53 FEE39 pKa = 4.45 RR40 pKa = 11.84 VYY42 pKa = 11.2 SSAMSPRR49 pKa = 11.84 ADD51 pKa = 2.92 LVVSTFIEE59 pKa = 4.79 IPCGRR64 pKa = 11.84 CLDD67 pKa = 3.88 CRR69 pKa = 11.84 LSYY72 pKa = 10.57 SRR74 pKa = 11.84 SWADD78 pKa = 3.39 RR79 pKa = 11.84 LVRR82 pKa = 11.84 EE83 pKa = 4.64 SKK85 pKa = 9.91 TSVSSLFVTFTYY97 pKa = 10.96 DD98 pKa = 4.22 DD99 pKa = 3.6 DD100 pKa = 4.06 HH101 pKa = 8.45 LPYY104 pKa = 9.98 HH105 pKa = 7.36 DD106 pKa = 4.49 YY107 pKa = 11.52 VNSEE111 pKa = 3.96 SGEE114 pKa = 4.2 LVSKK118 pKa = 10.14 PCLVKK123 pKa = 10.33 KK124 pKa = 9.89 HH125 pKa = 6.11 CQDD128 pKa = 2.89 FFKK131 pKa = 10.81 RR132 pKa = 11.84 LRR134 pKa = 11.84 KK135 pKa = 10.2 YY136 pKa = 10.56 FDD138 pKa = 4.02 GYY140 pKa = 10.05 NIRR143 pKa = 11.84 YY144 pKa = 8.77 FIAGEE149 pKa = 4.01 YY150 pKa = 10.57 GEE152 pKa = 4.63 TSARR156 pKa = 11.84 PHH158 pKa = 4.91 YY159 pKa = 10.42 HH160 pKa = 5.58 AVIYY164 pKa = 9.32 NLPLDD169 pKa = 4.07 KK170 pKa = 11.05 LDD172 pKa = 3.53 LQFYY176 pKa = 7.8 KK177 pKa = 10.98 TNFNGDD183 pKa = 3.75 LYY185 pKa = 9.39 YY186 pKa = 10.47 TSPVLEE192 pKa = 4.93 SIWTHH197 pKa = 5.76 GYY199 pKa = 9.81 VVLSDD204 pKa = 4.71 FSWQTGAYY212 pKa = 5.63 TARR215 pKa = 11.84 YY216 pKa = 8.46 ILKK219 pKa = 9.72 KK220 pKa = 10.52 QFGKK224 pKa = 10.76 ASDD227 pKa = 3.65 QFKK230 pKa = 9.69 LTGFPQEE237 pKa = 3.96 FVLMSRR243 pKa = 11.84 KK244 pKa = 9.48 PGLGRR249 pKa = 11.84 QYY251 pKa = 11.04 FNEE254 pKa = 4.58 NIDD257 pKa = 4.71 DD258 pKa = 5.19 LIAYY262 pKa = 9.61 DD263 pKa = 5.12 GDD265 pKa = 3.47 ICRR268 pKa = 11.84 PGQKK272 pKa = 9.64 SKK274 pKa = 10.42 PGRR277 pKa = 11.84 YY278 pKa = 8.72 FDD280 pKa = 5.41 SLIEE284 pKa = 4.47 DD285 pKa = 4.68 ADD287 pKa = 3.78 PEE289 pKa = 4.41 KK290 pKa = 11.41 YY291 pKa = 10.07 KK292 pKa = 10.66 DD293 pKa = 3.72 LKK295 pKa = 11.19 NNRR298 pKa = 11.84 QTVRR302 pKa = 11.84 DD303 pKa = 3.61 LFIAGKK309 pKa = 9.53 LAQTDD314 pKa = 3.49 KK315 pKa = 11.45 SYY317 pKa = 11.9 LDD319 pKa = 3.71 LLSVEE324 pKa = 4.13 DD325 pKa = 3.84 HH326 pKa = 7.03 YY327 pKa = 10.98 YY328 pKa = 7.85 TQRR331 pKa = 11.84 ALHH334 pKa = 6.77 LKK336 pKa = 10.51 RR337 pKa = 11.84 KK338 pKa = 9.85 DD339 pKa = 3.31 LL340 pKa = 4.04
Molecular weight: 39.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.373
IPC2_protein 7.658
IPC_protein 7.512
Toseland 7.19
ProMoST 8.053
Dawson 8.214
Bjellqvist 8.536
Wikipedia 8.156
Rodwell 8.244
Grimsley 7.234
Solomon 8.287
Lehninger 8.302
Nozaki 8.639
DTASelect 8.287
Thurlkill 8.346
EMBOSS 8.419
Sillero 8.639
Patrickios 3.872
IPC_peptide 8.287
IPC2_peptide 7.497
IPC2.peptide.svr19 7.692
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1362
84
558
272.4
30.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.461 ± 0.957
1.175 ± 0.601
6.902 ± 0.835
4.185 ± 0.742
4.993 ± 0.691
7.489 ± 1.054
1.762 ± 0.353
4.846 ± 0.624
5.507 ± 1.015
8.076 ± 0.494
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.129 ± 0.411
6.021 ± 0.811
5.36 ± 0.257
4.038 ± 0.542
3.818 ± 0.725
8.443 ± 1.235
5.8 ± 0.553
6.828 ± 0.689
1.101 ± 0.233
5.066 ± 0.711
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here