Pseudoxanthobacter soli DSM 19599
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4320 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M7ZNH1|A0A1M7ZNH1_9RHIZ NAD-dependent protein deacylase OS=Pseudoxanthobacter soli DSM 19599 OX=1123029 GN=cobB PE=3 SV=1
MM1 pKa = 7.47 SIDD4 pKa = 3.5 NGYY7 pKa = 9.84 DD8 pKa = 3.11 AQIAFISGVDD18 pKa = 3.78 GNGLLTPYY26 pKa = 10.73 AFSTWQSDD34 pKa = 4.26 TIPALYY40 pKa = 10.59 YY41 pKa = 9.97 PDD43 pKa = 4.21 SNEE46 pKa = 4.45 TKK48 pKa = 9.72 WGAPQPGTPATISYY62 pKa = 10.37 SFEE65 pKa = 4.63 DD66 pKa = 3.52 SSGWTATEE74 pKa = 3.73 RR75 pKa = 11.84 EE76 pKa = 4.34 AFVTAMALWSAVANVAFVEE95 pKa = 4.7 APDD98 pKa = 3.8 GTADD102 pKa = 3.48 FQIRR106 pKa = 11.84 RR107 pKa = 11.84 GTAGAFWTFDD117 pKa = 3.46 SVDD120 pKa = 3.81 TLPVGSDD127 pKa = 2.8 ILNSPVPGVPYY138 pKa = 10.64 LSIATDD144 pKa = 3.34 EE145 pKa = 4.77 GDD147 pKa = 3.94 FGPISTDD154 pKa = 3.37 LQVKK158 pKa = 9.75 GGYY161 pKa = 8.99 PFSTVVHH168 pKa = 6.0 EE169 pKa = 4.73 LGHH172 pKa = 5.9 GLGLGHH178 pKa = 6.97 SGPYY182 pKa = 9.58 NGNADD187 pKa = 3.88 PATQQFGPYY196 pKa = 9.7 DD197 pKa = 3.76 VNLWSLMSYY206 pKa = 10.35 INYY209 pKa = 9.73 RR210 pKa = 11.84 STNAAYY216 pKa = 9.75 YY217 pKa = 10.63 GSYY220 pKa = 9.44 TVTGTDD226 pKa = 2.64 WGQTPDD232 pKa = 3.22 GSNRR236 pKa = 11.84 DD237 pKa = 3.56 LQTPMILDD245 pKa = 3.75 IAAVQRR251 pKa = 11.84 LYY253 pKa = 10.85 GAPVDD258 pKa = 4.58 GPLSSGGQVFGFNSNIEE275 pKa = 4.27 GPLKK279 pKa = 10.77 AIFDD283 pKa = 4.11 FSINTTPVLTIWDD296 pKa = 4.11 GGTGNVLDD304 pKa = 4.15 VSGFTADD311 pKa = 2.93 AFIRR315 pKa = 11.84 LTPGSFSSVAGLANNIAIAPDD336 pKa = 3.92 TVIEE340 pKa = 4.06 TAIGGFGNDD349 pKa = 3.46 VIIGTEE355 pKa = 4.1 LNNVLIGNAGRR366 pKa = 11.84 DD367 pKa = 3.49 HH368 pKa = 7.17 IYY370 pKa = 11.03 GVVGSDD376 pKa = 3.84 WISGGPGGDD385 pKa = 3.25 FIVFGTSEE393 pKa = 4.22 NPFGSGGSMLADD405 pKa = 3.71 TLADD409 pKa = 3.85 LDD411 pKa = 4.19 GDD413 pKa = 4.32 SVAGLGLHH421 pKa = 5.66 NVIGILGAGLARR433 pKa = 11.84 ADD435 pKa = 3.15 IAVARR440 pKa = 11.84 TADD443 pKa = 3.7 GAIVSAGGSSFKK455 pKa = 10.64 IGGDD459 pKa = 3.36 LSGGDD464 pKa = 3.63 FMAVARR470 pKa = 11.84 QTNGQTHH477 pKa = 5.52 TAFSFVDD484 pKa = 3.72 YY485 pKa = 10.93 LPALAEE491 pKa = 4.38 GVSVAPGLINGIANPAFMAGDD512 pKa = 4.02 GSVGFSVQIEE522 pKa = 4.58 SAVSSYY528 pKa = 11.62 SNMLGYY534 pKa = 10.9 YY535 pKa = 9.69 SVSLNGTISDD545 pKa = 3.52 VHH547 pKa = 8.01 LLFEE551 pKa = 4.5 NTLEE555 pKa = 4.18 AAASGEE561 pKa = 4.33 TVNLGKK567 pKa = 9.83 PGDD570 pKa = 3.88 GQQIGFFLVQNGYY583 pKa = 9.9 EE584 pKa = 4.33 SYY586 pKa = 11.76 GDD588 pKa = 4.18 LPDD591 pKa = 5.06 DD592 pKa = 4.08 LSFVSTAGLSTEE604 pKa = 4.82 GGSPWVLYY612 pKa = 9.64 SQSRR616 pKa = 11.84 GFLSDD621 pKa = 2.97 AQVFHH626 pKa = 7.28 SYY628 pKa = 10.51 AAYY631 pKa = 10.63 NPDD634 pKa = 2.82 GKK636 pKa = 10.64 EE637 pKa = 3.78 QVLSGTIGGGGYY649 pKa = 10.31 LEE651 pKa = 4.88 VGFEE655 pKa = 5.05 DD656 pKa = 3.63 ILRR659 pKa = 11.84 DD660 pKa = 3.63 TGDD663 pKa = 3.29 NDD665 pKa = 3.62 YY666 pKa = 10.92 QDD668 pKa = 3.63 VVIAVRR674 pKa = 11.84 EE675 pKa = 4.04 SDD677 pKa = 3.87 GLFLVV682 pKa = 4.46
Molecular weight: 70.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.91
Patrickios 0.68
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A1M7ZMA5|A0A1M7ZMA5_9RHIZ Predicted ATP-dependent endonuclease of the OLD family contains P-loop ATPase and TOPRIM domains OS=Pseudoxanthobacter soli DSM 19599 OX=1123029 GN=SAMN02745172_02433 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 9.63 AHH4 pKa = 7.03 PVMTPPGVMKK14 pKa = 10.71 RR15 pKa = 11.84 PGATRR20 pKa = 11.84 RR21 pKa = 11.84 PMARR25 pKa = 11.84 NGRR28 pKa = 11.84 GTPRR32 pKa = 11.84 PGTLWVRR39 pKa = 11.84 RR40 pKa = 11.84 VGWLVLIWVASVAALGVVALVFRR63 pKa = 11.84 AVMSLAGMTPP73 pKa = 3.14
Molecular weight: 7.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.506
IPC2_protein 11.14
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.501
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.237
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4320
0
4320
1486866
39
4325
344.2
36.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.864 ± 0.059
0.735 ± 0.009
5.669 ± 0.034
5.345 ± 0.044
3.632 ± 0.024
9.348 ± 0.086
1.893 ± 0.021
4.975 ± 0.028
2.591 ± 0.029
9.945 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.233 ± 0.017
2.297 ± 0.028
5.442 ± 0.041
2.553 ± 0.02
7.285 ± 0.057
5.356 ± 0.05
5.481 ± 0.065
8.088 ± 0.03
1.227 ± 0.012
2.043 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here