Cephalotus follicularis (Albany pitcher plant)
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36397 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q3B545|A0A1Q3B545_CEPFO EamA domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_06639 PE=3 SV=1
MM1 pKa = 7.52 HH2 pKa = 7.16 EE3 pKa = 4.3 VDD5 pKa = 5.97 NVDD8 pKa = 3.66 LDD10 pKa = 3.72 QYY12 pKa = 11.84 SYY14 pKa = 11.96 LDD16 pKa = 4.32 LLADD20 pKa = 3.58 CWKK23 pKa = 10.69 FMFPKK28 pKa = 10.34 VYY30 pKa = 10.05 IDD32 pKa = 3.24 ITTKK36 pKa = 9.81 IGMMISNDD44 pKa = 3.81 RR45 pKa = 11.84 EE46 pKa = 4.26 LMDD49 pKa = 3.4 MFASHH54 pKa = 5.62 EE55 pKa = 4.24 HH56 pKa = 4.09 VHH58 pKa = 6.6 EE59 pKa = 4.56 INLFVKK65 pKa = 10.69 HH66 pKa = 6.42 SGRR69 pKa = 11.84 QIDD72 pKa = 3.94 FTLSRR77 pKa = 11.84 MPPAWNEE84 pKa = 3.85 HH85 pKa = 5.83 SDD87 pKa = 3.63 SDD89 pKa = 4.38 VEE91 pKa = 4.89 RR92 pKa = 11.84 SVDD95 pKa = 3.89 DD96 pKa = 4.06 DD97 pKa = 3.69 RR98 pKa = 11.84 CGYY101 pKa = 10.95 VEE103 pKa = 5.0 MSDD106 pKa = 5.6 NDD108 pKa = 4.04 DD109 pKa = 3.45 QCGDD113 pKa = 4.32 GIDD116 pKa = 4.62 EE117 pKa = 4.36 SDD119 pKa = 3.14 EE120 pKa = 4.33 GQNEE124 pKa = 4.49 VYY126 pKa = 10.42 DD127 pKa = 5.21 CSDD130 pKa = 3.24 EE131 pKa = 6.3 DD132 pKa = 3.96 DD133 pKa = 4.62 AEE135 pKa = 4.22 WMNTEE140 pKa = 4.52 EE141 pKa = 4.96 DD142 pKa = 4.3 NEE144 pKa = 4.14 DD145 pKa = 3.63 SPINGSDD152 pKa = 3.54 YY153 pKa = 10.85 EE154 pKa = 4.43 YY155 pKa = 10.95 EE156 pKa = 4.0 LEE158 pKa = 4.07 PWFDD162 pKa = 3.82 EE163 pKa = 4.9 DD164 pKa = 5.49 NEE166 pKa = 4.28 IMDD169 pKa = 4.0
Molecular weight: 19.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.821
Dawson 3.681
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.808
Patrickios 1.176
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|A0A1Q3CW69|A0A1Q3CW69_CEPFO Uncharacterized protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_27923 PE=4 SV=1
LL1 pKa = 7.11 GRR3 pKa = 11.84 PPHH6 pKa = 5.07 QQSLRR11 pKa = 11.84 RR12 pKa = 11.84 PPHH15 pKa = 4.57 QQSLGRR21 pKa = 11.84 PPQQPSLGRR30 pKa = 11.84 PPHH33 pKa = 4.87 QQSLRR38 pKa = 11.84 RR39 pKa = 11.84 PPHH42 pKa = 4.57 QQSLGRR48 pKa = 11.84 PPQQPSLVRR57 pKa = 11.84 PPHH60 pKa = 4.77 QQSLRR65 pKa = 11.84 RR66 pKa = 11.84 PPHH69 pKa = 4.57 QQSLGRR75 pKa = 11.84 PPQHH79 pKa = 7.01 PSLGRR84 pKa = 11.84 PPQQQSLRR92 pKa = 11.84 RR93 pKa = 11.84 QPHH96 pKa = 4.18 QQSLRR101 pKa = 11.84 RR102 pKa = 11.84 PPP104 pKa = 3.33
Molecular weight: 12.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.433
IPC_protein 13.042
Toseland 13.203
ProMoST 13.7
Dawson 13.203
Bjellqvist 13.203
Wikipedia 13.685
Rodwell 12.705
Grimsley 13.247
Solomon 13.7
Lehninger 13.598
Nozaki 13.203
DTASelect 13.203
Thurlkill 13.203
EMBOSS 13.7
Sillero 13.203
Patrickios 12.427
IPC_peptide 13.7
IPC2_peptide 12.691
IPC2.peptide.svr19 9.338
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36277
120
36397
11578339
8
5401
318.1
35.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.122 ± 0.012
1.905 ± 0.007
5.282 ± 0.009
6.488 ± 0.015
4.24 ± 0.008
6.072 ± 0.012
2.637 ± 0.007
5.745 ± 0.011
6.604 ± 0.013
9.814 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.543 ± 0.006
4.727 ± 0.01
4.734 ± 0.013
3.694 ± 0.01
5.231 ± 0.01
8.475 ± 0.017
5.044 ± 0.009
6.365 ± 0.01
1.391 ± 0.006
2.888 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here