Hubei picorna-like virus 55
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ97|A0A1L3KJ97_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 55 OX=1923137 PE=4 SV=1
MM1 pKa = 7.47 FGFAQASANSFLPIMEE17 pKa = 5.11 SYY19 pKa = 11.25 ASTAIYY25 pKa = 9.05 MDD27 pKa = 4.21 CSRR30 pKa = 11.84 ILYY33 pKa = 8.86 EE34 pKa = 4.48 VIPVCPEE41 pKa = 3.59 LTDD44 pKa = 2.9 ICLNFKK50 pKa = 8.75 TAQANEE56 pKa = 4.06 IFVEE60 pKa = 4.5 KK61 pKa = 10.41 PMPCSFDD68 pKa = 3.26 TKK70 pKa = 10.61 QGTTVTYY77 pKa = 8.71 TPTIFRR83 pKa = 11.84 IYY85 pKa = 9.84 EE86 pKa = 4.01 FYY88 pKa = 10.95 YY89 pKa = 10.46 KK90 pKa = 10.21 YY91 pKa = 10.1 VQYY94 pKa = 11.26 RR95 pKa = 11.84 LTYY98 pKa = 8.76 WNIPEE103 pKa = 4.82 DD104 pKa = 3.79 LQKK107 pKa = 11.24 KK108 pKa = 7.95 FLQMKK113 pKa = 8.71 TYY115 pKa = 9.45 TIRR118 pKa = 11.84 FPTVNQISGYY128 pKa = 10.22 DD129 pKa = 3.56 NPTALPNLPRR139 pKa = 11.84 GLRR142 pKa = 11.84 QLVFSPTPQYY152 pKa = 11.36 AVNPTFRR159 pKa = 11.84 GRR161 pKa = 11.84 MTVHH165 pKa = 6.21 TPALRR170 pKa = 11.84 LLLEE174 pKa = 4.08 RR175 pKa = 11.84 LRR177 pKa = 11.84 DD178 pKa = 3.51 EE179 pKa = 4.81 FGPVVVEE186 pKa = 3.79 IVISNAINSEE196 pKa = 3.63 PVIIVRR202 pKa = 11.84 VDD204 pKa = 3.53 LSSADD209 pKa = 4.06 DD210 pKa = 3.66 VVMLYY215 pKa = 10.72 VRR217 pKa = 11.84 VLDD220 pKa = 4.16 KK221 pKa = 11.39 APLYY225 pKa = 9.06 ATLRR229 pKa = 11.84 FPTQMLMINTIQSVYY244 pKa = 11.02 ASNEE248 pKa = 3.55 QTLTDD253 pKa = 3.87 DD254 pKa = 5.58 SEE256 pKa = 4.72 FEE258 pKa = 4.61 SNVIIANAGFAAAMIGGGALSGIGQGLGQYY288 pKa = 9.96 AQTKK292 pKa = 9.42 AQMHH296 pKa = 5.03 MQKK299 pKa = 10.5 EE300 pKa = 4.17 YY301 pKa = 11.3 LDD303 pKa = 3.72 WQRR306 pKa = 11.84 KK307 pKa = 6.06 KK308 pKa = 10.63 QGYY311 pKa = 9.94 DD312 pKa = 2.82 IDD314 pKa = 3.84 NQRR317 pKa = 11.84 YY318 pKa = 8.0 LQQSMFDD325 pKa = 3.87 FQSSRR330 pKa = 11.84 LDD332 pKa = 3.3 RR333 pKa = 11.84 EE334 pKa = 3.96 FQYY337 pKa = 11.16 GRR339 pKa = 11.84 EE340 pKa = 3.92 QQEE343 pKa = 4.1 RR344 pKa = 11.84 NINWGRR350 pKa = 11.84 EE351 pKa = 3.89 QQSAAFSQQNLYY363 pKa = 10.78 QDD365 pKa = 3.81 TQNKK369 pKa = 5.91 FTKK372 pKa = 10.3 EE373 pKa = 3.77 LVEE376 pKa = 4.37 TNLDD380 pKa = 3.19 SDD382 pKa = 3.48 IRR384 pKa = 11.84 RR385 pKa = 11.84 ARR387 pKa = 11.84 NAQQLAGFRR396 pKa = 11.84 TDD398 pKa = 3.12 ATVSGVNFVSGRR410 pKa = 11.84 GGLGHH415 pKa = 7.62 PDD417 pKa = 3.38 APRR420 pKa = 11.84 GASLPPRR427 pKa = 11.84 SASTQTNSPKK437 pKa = 10.41 VYY439 pKa = 8.66 LTRR442 pKa = 11.84 PTGYY446 pKa = 8.65 YY447 pKa = 9.4 TGPLSNEE454 pKa = 3.52 EE455 pKa = 4.31 RR456 pKa = 11.84 II457 pKa = 3.94
Molecular weight: 51.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.283
IPC2_protein 6.415
IPC_protein 6.62
Toseland 6.532
ProMoST 6.956
Dawson 7.044
Bjellqvist 6.854
Wikipedia 7.015
Rodwell 7.029
Grimsley 6.59
Solomon 7.102
Lehninger 7.102
Nozaki 7.146
DTASelect 7.293
Thurlkill 7.307
EMBOSS 7.366
Sillero 7.395
Patrickios 3.973
IPC_peptide 7.102
IPC2_peptide 6.868
IPC2.peptide.svr19 6.886
Protein with the highest isoelectric point:
>tr|A0A1L3KJ97|A0A1L3KJ97_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 55 OX=1923137 PE=4 SV=1
MM1 pKa = 7.33 SCSKK5 pKa = 10.72 ALEE8 pKa = 4.11 QGRR11 pKa = 11.84 IMPQISWVNTYY22 pKa = 10.82 KK23 pKa = 10.51 FVKK26 pKa = 9.12 NTIEE30 pKa = 3.77 TWLVGLVEE38 pKa = 4.61 NFLSPSEE45 pKa = 4.08 VTKK48 pKa = 10.89 ILMQLVFLITNIIVNVVTLFKK69 pKa = 10.88 SSTGVTMRR77 pKa = 11.84 LLSGANLLVTFQLVASAIANGDD99 pKa = 3.38 VGFDD103 pKa = 3.4 TNRR106 pKa = 11.84 ASDD109 pKa = 3.91 YY110 pKa = 10.44 LEE112 pKa = 4.1 CRR114 pKa = 11.84 ITEE117 pKa = 4.16 LEE119 pKa = 3.86 EE120 pKa = 4.15 EE121 pKa = 4.41 YY122 pKa = 11.17 ADD124 pKa = 4.05 RR125 pKa = 11.84 VFPNSAHH132 pKa = 6.88 AEE134 pKa = 4.17 VEE136 pKa = 4.29 QGWVQTTAVVFTAVFTAILAGYY158 pKa = 9.0 GISTKK163 pKa = 10.84 GIDD166 pKa = 3.35 GLTSVFRR173 pKa = 11.84 SFTATKK179 pKa = 10.46 NGVSSIKK186 pKa = 10.43 EE187 pKa = 3.78 IVQEE191 pKa = 4.3 AIKK194 pKa = 10.76 LIAEE198 pKa = 4.43 DD199 pKa = 4.73 KK200 pKa = 9.99 YY201 pKa = 11.71 DD202 pKa = 3.66 PVNIEE207 pKa = 3.58 KK208 pKa = 10.02 TKK210 pKa = 10.28 IRR212 pKa = 11.84 EE213 pKa = 4.26 LTEE216 pKa = 3.59 EE217 pKa = 3.91 AQEE220 pKa = 4.14 LVRR223 pKa = 11.84 LNIGAIACDD232 pKa = 3.33 NAVKK236 pKa = 10.35 EE237 pKa = 4.19 RR238 pKa = 11.84 FRR240 pKa = 11.84 TIIKK244 pKa = 10.02 DD245 pKa = 3.17 INQALLSFRR254 pKa = 11.84 HH255 pKa = 6.33 PDD257 pKa = 3.38 SANMRR262 pKa = 11.84 SLLGTLCTDD271 pKa = 4.15 LNKK274 pKa = 10.48 KK275 pKa = 10.32 LSEE278 pKa = 4.09 VDD280 pKa = 3.27 SLIPTRR286 pKa = 11.84 QVTAGVFLLGDD297 pKa = 3.82 PGVGKK302 pKa = 8.62 STLAAYY308 pKa = 9.59 ISKK311 pKa = 10.37 VMAKK315 pKa = 9.9 EE316 pKa = 3.89 FGWRR320 pKa = 11.84 DD321 pKa = 3.29 SIYY324 pKa = 10.53 NVSPGVRR331 pKa = 11.84 FYY333 pKa = 11.37 EE334 pKa = 4.59 PYY336 pKa = 10.6 LNEE339 pKa = 4.49 DD340 pKa = 3.39 FGIVNEE346 pKa = 4.36 FASTVTPDD354 pKa = 4.87 LFSTDD359 pKa = 3.19 ANRR362 pKa = 11.84 ICSSDD367 pKa = 3.25 PFNFEE372 pKa = 4.08 SAGLSGKK379 pKa = 10.28 VSMCRR384 pKa = 11.84 LKK386 pKa = 10.59 MLFVTSNNWCPEE398 pKa = 3.31 IRR400 pKa = 11.84 EE401 pKa = 4.59 LLPDD405 pKa = 4.98 SIPAFWDD412 pKa = 3.13 RR413 pKa = 11.84 FIIFEE418 pKa = 4.23 VKK420 pKa = 10.25 DD421 pKa = 3.59 PKK423 pKa = 10.56 IEE425 pKa = 3.92 NRR427 pKa = 11.84 RR428 pKa = 11.84 MRR430 pKa = 11.84 AYY432 pKa = 9.87 HH433 pKa = 6.04 RR434 pKa = 11.84 KK435 pKa = 9.41 EE436 pKa = 4.28 DD437 pKa = 4.2 FSHH440 pKa = 5.74 LTFNIVEE447 pKa = 4.32 QYY449 pKa = 8.65 SQRR452 pKa = 11.84 GEE454 pKa = 3.93 AFKK457 pKa = 10.67 FRR459 pKa = 11.84 YY460 pKa = 8.86 VFKK463 pKa = 10.66 DD464 pKa = 3.08 LTVFDD469 pKa = 3.72 VMEE472 pKa = 4.19 ILRR475 pKa = 11.84 TKK477 pKa = 10.59 LALEE481 pKa = 4.56 TIAFHH486 pKa = 7.1 KK487 pKa = 10.36 RR488 pKa = 11.84 VEE490 pKa = 4.21 QPLPIDD496 pKa = 3.82 FKK498 pKa = 11.64 EE499 pKa = 5.2 DD500 pKa = 2.88 ILKK503 pKa = 10.41 AANPPEE509 pKa = 3.99 GSYY512 pKa = 10.51 AYY514 pKa = 9.76 KK515 pKa = 10.82 YY516 pKa = 10.34 FGFDD520 pKa = 4.3 AVVQPNAVGRR530 pKa = 11.84 NFCILRR536 pKa = 11.84 LQGPEE541 pKa = 4.01 GTGKK545 pKa = 7.26 TTIAKK550 pKa = 9.88 KK551 pKa = 10.14 IAEE554 pKa = 4.29 KK555 pKa = 9.14 WQKK558 pKa = 8.37 LTKK561 pKa = 10.43 SHH563 pKa = 7.45 DD564 pKa = 3.4 GPSRR568 pKa = 11.84 YY569 pKa = 9.6 KK570 pKa = 10.23 IQLSQSLKK578 pKa = 11.03 DD579 pKa = 3.33 FTPEE583 pKa = 3.67 KK584 pKa = 10.37 KK585 pKa = 10.04 PYY587 pKa = 10.09 IYY589 pKa = 10.38 ILDD592 pKa = 3.97 DD593 pKa = 3.53 WVSSNMTVTEE603 pKa = 3.9 SEE605 pKa = 4.58 EE606 pKa = 4.15 YY607 pKa = 8.66 VKK609 pKa = 11.01 KK610 pKa = 10.53 MNSTTTDD617 pKa = 3.51 SIFVITTNEE626 pKa = 3.92 VIKK629 pKa = 10.74 RR630 pKa = 11.84 RR631 pKa = 11.84 MPWFDD636 pKa = 3.06 LGARR640 pKa = 11.84 LVAIMLNNEE649 pKa = 3.74 YY650 pKa = 10.17 EE651 pKa = 4.38 YY652 pKa = 10.24 PYY654 pKa = 11.48 LMDD657 pKa = 4.87 NFSKK661 pKa = 10.56 LYY663 pKa = 8.89 PGIRR667 pKa = 11.84 RR668 pKa = 11.84 RR669 pKa = 11.84 IGLDD673 pKa = 2.96 KK674 pKa = 11.04 LYY676 pKa = 10.71 LCEE679 pKa = 4.87 GKK681 pKa = 9.83 LHH683 pKa = 5.88 QLPSLEE689 pKa = 4.08 FCLTIDD695 pKa = 3.45 TTTNGHH701 pKa = 6.43 IKK703 pKa = 10.39 HH704 pKa = 6.41 NYY706 pKa = 7.57 NNVGVSYY713 pKa = 10.67 ILEE716 pKa = 4.25 HH717 pKa = 6.61 LKK719 pKa = 10.49 KK720 pKa = 10.39 KK721 pKa = 9.72 YY722 pKa = 9.69 MAMLEE727 pKa = 3.83 DD728 pKa = 4.5 RR729 pKa = 11.84 YY730 pKa = 11.18 NVVVLNHH737 pKa = 6.41 RR738 pKa = 11.84 PEE740 pKa = 4.27 TEE742 pKa = 3.77 QTHH745 pKa = 5.82 VDD747 pKa = 3.85 FEE749 pKa = 5.73 LIVDD753 pKa = 3.84 STEE756 pKa = 4.0 TLFSKK761 pKa = 10.74 LRR763 pKa = 11.84 TSLGASSLFLNPTSKK778 pKa = 11.07 ASLKK782 pKa = 9.9 VSDD785 pKa = 4.87 RR786 pKa = 11.84 IVEE789 pKa = 4.12 GVMNVNPEE797 pKa = 3.7 KK798 pKa = 10.62 SIRR801 pKa = 11.84 PSDD804 pKa = 3.84 FVCTEE809 pKa = 3.61 IFKK812 pKa = 11.2 EE813 pKa = 4.18 EE814 pKa = 4.1 VPLQDD819 pKa = 3.11 RR820 pKa = 11.84 YY821 pKa = 10.55 SAIVEE826 pKa = 4.48 RR827 pKa = 11.84 ITRR830 pKa = 11.84 VLLQYY835 pKa = 10.85 FSDD838 pKa = 3.34 PTFRR842 pKa = 11.84 VTIKK846 pKa = 8.23 QTKK849 pKa = 7.42 EE850 pKa = 3.64 CVYY853 pKa = 10.19 SEE855 pKa = 4.32 KK856 pKa = 10.97 GVVYY860 pKa = 9.84 YY861 pKa = 11.11 YY862 pKa = 11.05 KK863 pKa = 10.73 DD864 pKa = 3.99 DD865 pKa = 3.93 DD866 pKa = 4.05 TDD868 pKa = 3.35 IVTVKK873 pKa = 11.05 GEE875 pKa = 4.85 DD876 pKa = 2.97 ILLQINTPKK885 pKa = 10.71 LINAVDD891 pKa = 4.58 FARR894 pKa = 11.84 SLSEE898 pKa = 5.32 DD899 pKa = 3.16 KK900 pKa = 11.3 LEE902 pKa = 5.24 SKK904 pKa = 10.59 FQDD907 pKa = 3.31 SLKK910 pKa = 10.63 QLTTTEE916 pKa = 4.12 YY917 pKa = 11.14 LVLEE921 pKa = 5.04 DD922 pKa = 3.53 YY923 pKa = 10.52 FKK925 pKa = 11.23 RR926 pKa = 11.84 SIRR929 pKa = 11.84 KK930 pKa = 8.1 QAFNPFITKK939 pKa = 10.04 VIKK942 pKa = 10.6 EE943 pKa = 3.9 EE944 pKa = 4.17 MKK946 pKa = 10.52 FKK948 pKa = 10.68 RR949 pKa = 11.84 LKK951 pKa = 10.7 DD952 pKa = 3.31 PSYY955 pKa = 11.38 INGLLTIKK963 pKa = 10.49 SNKK966 pKa = 8.89 VLVATVCVLVGATTLGGIYY985 pKa = 10.39 ALYY988 pKa = 10.68 KK989 pKa = 10.13 LVRR992 pKa = 11.84 PMFQEE997 pKa = 3.93 MAKK1000 pKa = 10.34 KK1001 pKa = 8.76 EE1002 pKa = 4.42 TVVDD1006 pKa = 3.45 NAFANTGSTPPKK1018 pKa = 9.73 RR1019 pKa = 11.84 AMEE1022 pKa = 4.18 NQSLKK1027 pKa = 10.92 DD1028 pKa = 3.53 KK1029 pKa = 10.48 RR1030 pKa = 11.84 NLGATVTTAIKK1041 pKa = 10.67 SNSIHH1046 pKa = 6.23 SKK1048 pKa = 10.12 HH1049 pKa = 6.29 RR1050 pKa = 11.84 GKK1052 pKa = 10.83 VIPNYY1057 pKa = 10.08 KK1058 pKa = 9.86 SNKK1061 pKa = 8.17 SSSGSEE1067 pKa = 3.69 EE1068 pKa = 3.63 EE1069 pKa = 5.1 LIINSPHH1076 pKa = 5.45 TKK1078 pKa = 9.92 HH1079 pKa = 6.44 RR1080 pKa = 11.84 GKK1082 pKa = 10.63 VIPNMEE1088 pKa = 3.99 TAEE1091 pKa = 4.06 ILEE1094 pKa = 4.65 TVQPTDD1100 pKa = 2.91 KK1101 pKa = 10.6 TLNEE1105 pKa = 3.99 QHH1107 pKa = 5.89 EE1108 pKa = 5.19 HH1109 pKa = 5.32 ICKK1112 pKa = 10.28 SCSCLYY1118 pKa = 10.11 IHH1120 pKa = 6.08 SHH1122 pKa = 5.71 KK1123 pKa = 10.02 MNKK1126 pKa = 9.33 NFIHH1130 pKa = 6.3 PQHH1133 pKa = 6.82 SKK1135 pKa = 9.63 QCPNYY1140 pKa = 10.17 LCKK1143 pKa = 10.31 EE1144 pKa = 3.92 YY1145 pKa = 10.99 HH1146 pKa = 6.44 NGFNKK1151 pKa = 10.34 SNAVLIQLNFIEE1163 pKa = 4.83 TSLEE1167 pKa = 3.87 PLTPNALAKK1176 pKa = 10.59 IMGEE1180 pKa = 3.75 EE1181 pKa = 4.02 FSYY1184 pKa = 11.58 VKK1186 pKa = 10.15 TGLLKK1191 pKa = 10.72 FFSTLTEE1198 pKa = 3.94 EE1199 pKa = 4.39 QRR1201 pKa = 11.84 KK1202 pKa = 7.73 QVYY1205 pKa = 10.42 DD1206 pKa = 4.56 EE1207 pKa = 3.63 IHH1209 pKa = 4.86 QAKK1212 pKa = 9.86 RR1213 pKa = 11.84 GNDD1216 pKa = 3.03 RR1217 pKa = 11.84 TFKK1220 pKa = 10.65 RR1221 pKa = 11.84 LTRR1224 pKa = 11.84 RR1225 pKa = 11.84 YY1226 pKa = 8.23 PQLEE1230 pKa = 4.4 EE1231 pKa = 3.7 YY1232 pKa = 8.62 VQRR1235 pKa = 11.84 VIKK1238 pKa = 10.9 ANMLCEE1244 pKa = 4.09 ADD1246 pKa = 3.91 MIEE1249 pKa = 4.42 SPEE1252 pKa = 4.15 SEE1254 pKa = 4.55 LEE1256 pKa = 3.87 ALHH1259 pKa = 7.18 RR1260 pKa = 11.84 KK1261 pKa = 7.44 LTKK1264 pKa = 10.34 CYY1266 pKa = 10.22 VQVSYY1271 pKa = 11.49 AGFKK1275 pKa = 10.46 AYY1277 pKa = 10.26 ALHH1280 pKa = 6.14 VGKK1283 pKa = 10.93 GMFLCVSHH1291 pKa = 6.97 CFDD1294 pKa = 3.3 EE1295 pKa = 4.58 VGRR1298 pKa = 11.84 TVLLKK1303 pKa = 10.48 SEE1305 pKa = 4.13 NVSYY1309 pKa = 10.54 PSIVLSIVRR1318 pKa = 11.84 EE1319 pKa = 3.68 RR1320 pKa = 11.84 DD1321 pKa = 3.13 LAVVYY1326 pKa = 10.85 CKK1328 pKa = 10.58 DD1329 pKa = 3.88 LNNLPSGKK1337 pKa = 10.1 KK1338 pKa = 10.06 YY1339 pKa = 8.1 FTRR1342 pKa = 11.84 DD1343 pKa = 3.32 SNNLSLAGYY1352 pKa = 8.68 FMRR1355 pKa = 11.84 CGPQLAIVSGNLEE1368 pKa = 3.84 YY1369 pKa = 10.78 KK1370 pKa = 10.24 EE1371 pKa = 3.99 YY1372 pKa = 11.08 QEE1374 pKa = 5.86 FIDD1377 pKa = 4.91 GSCSNEE1383 pKa = 3.88 HH1384 pKa = 6.41 YY1385 pKa = 9.75 KK1386 pKa = 10.77 PCMEE1390 pKa = 4.01 VLKK1393 pKa = 10.54 LRR1395 pKa = 11.84 RR1396 pKa = 11.84 VGLMVEE1402 pKa = 4.46 EE1403 pKa = 4.82 IIKK1406 pKa = 10.83 KK1407 pKa = 9.75 GDD1409 pKa = 3.33 CGFPFMCKK1417 pKa = 9.78 VGGSIKK1423 pKa = 10.78 LIGFHH1428 pKa = 6.36 NAYY1431 pKa = 6.85 KK1432 pKa = 9.2 THH1434 pKa = 6.79 AIVYY1438 pKa = 9.47 GSFISQPLIDD1448 pKa = 4.77 EE1449 pKa = 4.57 LCEE1452 pKa = 4.23 VITPNADD1459 pKa = 2.88 IPSPITVVRR1468 pKa = 11.84 VPVKK1472 pKa = 10.51 RR1473 pKa = 11.84 EE1474 pKa = 3.7 NEE1476 pKa = 4.02 EE1477 pKa = 4.07 TIHH1480 pKa = 8.04 IEE1482 pKa = 3.2 MSMPHH1487 pKa = 6.31 EE1488 pKa = 4.21 YY1489 pKa = 10.63 ARR1491 pKa = 11.84 EE1492 pKa = 3.66 FLNCIKK1498 pKa = 9.52 PDD1500 pKa = 3.21 PMMPLEE1506 pKa = 4.3 STLTSIGYY1514 pKa = 8.82 NPKK1517 pKa = 10.17 FKK1519 pKa = 10.88 NFNNMKK1525 pKa = 8.77 EE1526 pKa = 3.76 KK1527 pKa = 10.37 HH1528 pKa = 4.87 YY1529 pKa = 8.83 THH1531 pKa = 7.18 EE1532 pKa = 4.89 IEE1534 pKa = 5.26 LEE1536 pKa = 4.13 NPNMQLPAAFTNRR1549 pKa = 11.84 YY1550 pKa = 6.83 VTDD1553 pKa = 4.33 FSEE1556 pKa = 3.86 ITLNGKK1562 pKa = 9.1 GIPDD1566 pKa = 4.37 PLWTQAMKK1574 pKa = 10.91 YY1575 pKa = 7.11 GTRR1578 pKa = 11.84 KK1579 pKa = 9.9 KK1580 pKa = 10.7 NYY1582 pKa = 9.25 DD1583 pKa = 3.21 EE1584 pKa = 6.16 DD1585 pKa = 3.93 IFRR1588 pKa = 11.84 HH1589 pKa = 4.73 AVEE1592 pKa = 4.48 MVVDD1596 pKa = 4.11 YY1597 pKa = 11.74 NKK1599 pKa = 9.48 LTYY1602 pKa = 10.64 CNSKK1606 pKa = 8.84 PFKK1609 pKa = 10.13 MLTEE1613 pKa = 4.45 FEE1615 pKa = 4.34 ALNGRR1620 pKa = 11.84 TEE1622 pKa = 4.14 PFLSNVDD1629 pKa = 3.32 IKK1631 pKa = 10.39 TSAGPYY1637 pKa = 9.47 AKK1639 pKa = 10.01 YY1640 pKa = 10.02 FYY1642 pKa = 11.11 GIMTKK1647 pKa = 9.23 QEE1649 pKa = 4.07 FLEE1652 pKa = 4.14 ITYY1655 pKa = 10.29 HH1656 pKa = 6.26 DD1657 pKa = 4.37 EE1658 pKa = 3.84 KK1659 pKa = 10.62 PIYY1662 pKa = 10.58 SFAHH1666 pKa = 5.51 NKK1668 pKa = 9.35 IGQNIRR1674 pKa = 11.84 NHH1676 pKa = 6.46 LKK1678 pKa = 7.62 TQRR1681 pKa = 11.84 SLLEE1685 pKa = 4.07 NYY1687 pKa = 8.94 GIPPCLISQDD1697 pKa = 3.48 NAKK1700 pKa = 10.24 VEE1702 pKa = 4.56 NIDD1705 pKa = 3.91 KK1706 pKa = 10.82 EE1707 pKa = 4.19 KK1708 pKa = 11.14 AEE1710 pKa = 4.1 KK1711 pKa = 10.81 GKK1713 pKa = 10.33 VRR1715 pKa = 11.84 LFNNVDD1721 pKa = 3.3 PSINALLKK1729 pKa = 10.77 IMFGDD1734 pKa = 3.68 WFSRR1738 pKa = 11.84 AMAKK1742 pKa = 10.24 SSEE1745 pKa = 4.12 GYY1747 pKa = 9.71 YY1748 pKa = 10.61 AIGQNPYY1755 pKa = 7.74 TTSTEE1760 pKa = 3.13 IWHH1763 pKa = 6.62 RR1764 pKa = 11.84 FSTKK1768 pKa = 8.94 QGKK1771 pKa = 8.54 ILNTDD1776 pKa = 4.37 FKK1778 pKa = 11.56 SFDD1781 pKa = 3.4 KK1782 pKa = 11.3 LLITEE1787 pKa = 5.45 LIEE1790 pKa = 4.08 AFCYY1794 pKa = 9.71 IAGRR1798 pKa = 11.84 LTKK1801 pKa = 10.29 NEE1803 pKa = 4.09 RR1804 pKa = 11.84 LEE1806 pKa = 4.45 EE1807 pKa = 3.99 LPMIYY1812 pKa = 10.13 DD1813 pKa = 4.26 ALALTLTHH1821 pKa = 7.64 AIHH1824 pKa = 6.95 LLNGSVYY1831 pKa = 10.26 VVHH1834 pKa = 6.86 NGNEE1838 pKa = 3.97 SGTFVTTLLNCVSVHH1853 pKa = 6.97 IIFNYY1858 pKa = 10.54 SFIKK1862 pKa = 10.17 CWNKK1866 pKa = 8.38 VPHH1869 pKa = 6.43 YY1870 pKa = 10.47 IGIKK1874 pKa = 10.19 PMLNDD1879 pKa = 2.69 IMSRR1883 pKa = 11.84 SEE1885 pKa = 3.83 LAILGDD1891 pKa = 4.41 DD1892 pKa = 3.41 KK1893 pKa = 11.4 TQIVSKK1899 pKa = 9.59 DD1900 pKa = 3.01 IPMTEE1905 pKa = 4.0 EE1906 pKa = 3.68 EE1907 pKa = 5.1 LIEE1910 pKa = 4.2 IAASLGMEE1918 pKa = 4.25 CTPAKK1923 pKa = 10.44 GGEE1926 pKa = 4.09 DD1927 pKa = 3.25 SGNEE1931 pKa = 3.49 INFCSRR1937 pKa = 11.84 VLEE1940 pKa = 4.49 WNEE1943 pKa = 3.62 HH1944 pKa = 5.35 DD1945 pKa = 3.46 QVVYY1949 pKa = 10.09 PKK1951 pKa = 10.1 LKK1953 pKa = 9.45 KK1954 pKa = 10.42 SSIIGLLYY1962 pKa = 10.03 WFVSFEE1968 pKa = 4.21 KK1969 pKa = 10.95 NQVRR1973 pKa = 11.84 DD1974 pKa = 3.43 NLMICLFEE1982 pKa = 6.2 ASLHH1986 pKa = 6.94 DD1987 pKa = 3.56 EE1988 pKa = 4.23 AFYY1991 pKa = 11.35 DD1992 pKa = 4.3 SVFRR1996 pKa = 11.84 DD1997 pKa = 3.46 AMVVATAMGVDD2008 pKa = 3.19 IRR2010 pKa = 11.84 TIPFTNYY2017 pKa = 7.57 QQSRR2021 pKa = 11.84 KK2022 pKa = 8.68 RR2023 pKa = 11.84 LRR2025 pKa = 11.84 SMLMNDD2031 pKa = 4.48 FEE2033 pKa = 4.43 YY2034 pKa = 10.98 QQISEE2039 pKa = 4.22 LAVRR2043 pKa = 11.84 KK2044 pKa = 7.85 TKK2046 pKa = 10.67 LFEE2049 pKa = 4.75 DD2050 pKa = 4.3 FCEE2053 pKa = 3.84 LSKK2056 pKa = 10.56 FLKK2059 pKa = 10.44 VSRR2062 pKa = 11.84 TLEE2065 pKa = 4.23 NKK2067 pKa = 9.87 VEE2069 pKa = 4.18 TASAAQIVEE2078 pKa = 4.21 YY2079 pKa = 10.86 KK2080 pKa = 10.41 EE2081 pKa = 4.95 LQDD2084 pKa = 3.54 CSRR2087 pKa = 11.84 VLRR2090 pKa = 11.84 KK2091 pKa = 9.75 RR2092 pKa = 11.84 KK2093 pKa = 9.82 SSTAKK2098 pKa = 10.32 RR2099 pKa = 11.84 ITRR2102 pKa = 11.84 EE2103 pKa = 3.85 QVDD2106 pKa = 4.07 EE2107 pKa = 4.75 GEE2109 pKa = 4.45 MASFSHH2115 pKa = 6.54 TSNPISACNEE2125 pKa = 4.2 LISKK2129 pKa = 10.35 LKK2131 pKa = 9.99 LDD2133 pKa = 4.36 KK2134 pKa = 10.86 PIEE2137 pKa = 4.3 TYY2139 pKa = 10.53 EE2140 pKa = 4.1 RR2141 pKa = 11.84 TGSDD2145 pKa = 3.22 HH2146 pKa = 7.11 EE2147 pKa = 4.3 PTYY2150 pKa = 11.3 SCTLRR2155 pKa = 11.84 YY2156 pKa = 8.15 TGRR2159 pKa = 11.84 EE2160 pKa = 3.83 FGGTGPSKK2168 pKa = 9.95 TSAKK2172 pKa = 8.29 TQAFGALRR2180 pKa = 11.84 VFLDD2184 pKa = 5.01 DD2185 pKa = 6.27 NIICNAACEE2194 pKa = 4.22 EE2195 pKa = 4.44 QVQSAHH2201 pKa = 5.64 EE2202 pKa = 4.56 KK2203 pKa = 9.58 IKK2205 pKa = 9.82 KK2206 pKa = 6.81 TAITAFCFTMEE2217 pKa = 4.12 AHH2219 pKa = 6.88 LEE2221 pKa = 4.09 AAKK2224 pKa = 10.7 SLSDD2228 pKa = 3.18 GKK2230 pKa = 10.92 KK2231 pKa = 9.14 YY2232 pKa = 10.98 VIIVASRR2239 pKa = 11.84 LGIDD2243 pKa = 3.27 NVEE2246 pKa = 4.31 EE2247 pKa = 3.62 IGGYY2251 pKa = 9.26 IRR2253 pKa = 11.84 FYY2255 pKa = 11.2 RR2256 pKa = 11.84 NGNCYY2261 pKa = 9.91 VLSPIIEE2268 pKa = 3.97 RR2269 pKa = 11.84 LGRR2272 pKa = 11.84 KK2273 pKa = 6.53 TAAACYY2279 pKa = 9.89 ARR2281 pKa = 11.84 YY2282 pKa = 9.11 PGVTYY2287 pKa = 7.96 DD2288 pKa = 3.49 TKK2290 pKa = 10.48 TKK2292 pKa = 9.93 EE2293 pKa = 3.75 VTYY2296 pKa = 10.12 DD2297 pKa = 3.01 VHH2299 pKa = 8.46 ASTSQYY2305 pKa = 9.84 VLRR2308 pKa = 11.84 DD2309 pKa = 3.39 IDD2311 pKa = 3.73 EE2312 pKa = 4.32 KK2313 pKa = 10.74 TYY2315 pKa = 11.14 SDD2317 pKa = 3.67 EE2318 pKa = 5.45 DD2319 pKa = 4.19 GEE2321 pKa = 4.53 LVANATRR2328 pKa = 11.84 DD2329 pKa = 3.32 IPIVGEE2335 pKa = 4.17 EE2336 pKa = 4.34 DD2337 pKa = 3.24 SGTPGKK2343 pKa = 10.04 MPVTSVMDD2351 pKa = 3.66 SDD2353 pKa = 3.46 KK2354 pKa = 11.66 AMYY2357 pKa = 9.71 QPIVMNRR2364 pKa = 11.84 SAPNNSWLKK2373 pKa = 10.93 AGGITFNISDD2383 pKa = 4.12 LAYY2386 pKa = 10.57 NQFVGCNKK2394 pKa = 9.4 QVQISDD2400 pKa = 3.32 GKK2402 pKa = 10.35 AAGSILAQIPYY2413 pKa = 10.32 DD2414 pKa = 3.55 PVSNEE2419 pKa = 3.32 FANAYY2424 pKa = 8.41 IQNLVLLHH2432 pKa = 6.3 GRR2434 pKa = 11.84 MTGDD2438 pKa = 2.61 WMVKK2442 pKa = 8.08 ITCPGNPGMQCSIGVAWVPSKK2463 pKa = 10.74 IAGEE2467 pKa = 4.43 TIQIDD2472 pKa = 3.74 QATKK2476 pKa = 9.87 YY2477 pKa = 10.97 AFFTTGVSSEE2487 pKa = 3.84 WSHH2490 pKa = 6.18 TIVMTDD2496 pKa = 2.84 ARR2498 pKa = 11.84 QSSFYY2503 pKa = 10.08 RR2504 pKa = 11.84 TIQRR2508 pKa = 11.84 DD2509 pKa = 3.78 SKK2511 pKa = 9.67 KK2512 pKa = 7.84 TTVTTDD2518 pKa = 3.25 DD2519 pKa = 3.67 MPHH2522 pKa = 5.71 VVVYY2526 pKa = 10.52 CEE2528 pKa = 4.15 TPATSVFTEE2537 pKa = 4.18 KK2538 pKa = 10.69 KK2539 pKa = 8.91 STFLTFFSKK2548 pKa = 10.94 LCDD2551 pKa = 3.71 EE2552 pKa = 5.15 KK2553 pKa = 11.54 DD2554 pKa = 3.5 LLFNPSIKK2562 pKa = 10.3 PFVLADD2568 pKa = 3.61 PQASVKK2574 pKa = 10.08 FDD2576 pKa = 3.2 ATKK2579 pKa = 10.85 GYY2581 pKa = 8.14 TLNFF2585 pKa = 3.77
Molecular weight: 293.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.49
IPC2_protein 6.707
IPC_protein 6.634
Toseland 6.634
ProMoST 7.146
Dawson 7.161
Bjellqvist 7.351
Wikipedia 7.117
Rodwell 7.176
Grimsley 6.722
Solomon 7.161
Lehninger 7.176
Nozaki 7.614
DTASelect 7.468
Thurlkill 7.512
EMBOSS 7.497
Sillero 7.629
Patrickios 4.19
IPC_peptide 7.176
IPC2_peptide 6.971
IPC2.peptide.svr19 6.86
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3042
457
2585
1521.0
172.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.95 ± 0.514
1.841 ± 0.472
4.865 ± 0.025
6.969 ± 0.946
4.931 ± 0.05
5.03 ± 0.322
1.874 ± 0.595
6.739 ± 0.513
7.068 ± 1.849
8.185 ± 0.15
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.564 ± 0.137
5.358 ± 0.052
4.306 ± 0.675
3.682 ± 1.942
4.767 ± 0.878
7.101 ± 0.262
7.002 ± 0.214
6.673 ± 0.374
0.723 ± 0.033
4.372 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here