Hubei tombus-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGS5|A0A1L3KGS5_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 3 OX=1923277 PE=4 SV=1
MM1 pKa = 7.27EE2 pKa = 5.94RR3 pKa = 11.84SLANAVQLVTATKK16 pKa = 10.45GRR18 pKa = 11.84TRR20 pKa = 11.84HH21 pKa = 5.63YY22 pKa = 9.75LPTMITLTDD31 pKa = 3.47NTEE34 pKa = 3.88LCFFAVCIVSVLYY47 pKa = 9.54TIYY50 pKa = 10.31QLPRR54 pKa = 11.84FVTWVCFVVWCWWNGVVQVSAQQEE78 pKa = 4.1RR79 pKa = 11.84MGARR83 pKa = 11.84LAFYY87 pKa = 10.66ADD89 pKa = 3.87EE90 pKa = 4.81EE91 pKa = 4.87DD92 pKa = 4.98PLDD95 pKa = 4.24LDD97 pKa = 4.8AVSEE101 pKa = 4.22LCEE104 pKa = 4.93DD105 pKa = 3.59DD106 pKa = 3.53VGRR109 pKa = 11.84STRR112 pKa = 11.84RR113 pKa = 11.84VRR115 pKa = 11.84RR116 pKa = 11.84SRR118 pKa = 11.84KK119 pKa = 9.64APFAAWLVEE128 pKa = 4.65EE129 pKa = 4.56IRR131 pKa = 11.84GAHH134 pKa = 6.99LSQCSRR140 pKa = 11.84TDD142 pKa = 3.26ANVLIFEE149 pKa = 4.75RR150 pKa = 11.84YY151 pKa = 8.84ARR153 pKa = 11.84SIMAEE158 pKa = 4.05HH159 pKa = 6.61NVRR162 pKa = 11.84PSDD165 pKa = 3.51AAKK168 pKa = 9.63VLPYY172 pKa = 9.99ATILFFEE179 pKa = 4.98HH180 pKa = 7.37RR181 pKa = 11.84SFDD184 pKa = 3.46QIAAVGTTQSSAFVSARR201 pKa = 11.84RR202 pKa = 11.84DD203 pKa = 3.41FAAKK207 pKa = 10.02YY208 pKa = 9.34VGRR211 pKa = 11.84SGWFSFGNASS221 pKa = 3.26

Molecular weight: 25.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGS5|A0A1L3KGS5_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 3 OX=1923277 PE=4 SV=1
MM1 pKa = 7.56APFMSALGIPFGSRR15 pKa = 11.84SVYY18 pKa = 10.59FLMSRR23 pKa = 11.84LLRR26 pKa = 11.84NASMRR31 pKa = 11.84AFLSVTVVTSLPKK44 pKa = 9.99GVWYY48 pKa = 10.71SVVLYY53 pKa = 10.79SCVGTLNSWSWDD65 pKa = 3.59DD66 pKa = 3.92LKK68 pKa = 11.06LTIGSTGLMAQNGGFALNTSVLNKK92 pKa = 9.64MPRR95 pKa = 11.84DD96 pKa = 3.83CTAPRR101 pKa = 11.84AWAWVQRR108 pKa = 11.84VPVDD112 pKa = 3.79VQSSSCGWIQYY123 pKa = 7.28TQHH126 pKa = 7.02KK127 pKa = 8.95MPKK130 pKa = 9.12KK131 pKa = 8.42NNKK134 pKa = 9.22SATSHH139 pKa = 5.06VFRR142 pKa = 11.84GCRR145 pKa = 11.84EE146 pKa = 3.86IFLVPAPRR154 pKa = 11.84AGVNNTVFSLVSASDD169 pKa = 3.67GSAAFAQVLCPLGLTSVKK187 pKa = 10.51LGTSTFTAGSYY198 pKa = 11.43GNVTGPPLRR207 pKa = 11.84GLFNRR212 pKa = 11.84ASDD215 pKa = 3.91FQWYY219 pKa = 9.34RR220 pKa = 11.84VTRR223 pKa = 11.84AKK225 pKa = 10.38FVFVGAVGSTATGVLTMNAYY245 pKa = 10.47SDD247 pKa = 4.28PYY249 pKa = 10.71DD250 pKa = 3.39IAIVGSAATMAGPSNRR266 pKa = 11.84TFDD269 pKa = 4.6LASSTNKK276 pKa = 9.71EE277 pKa = 3.6LSIPVPVDD285 pKa = 3.48STWKK289 pKa = 10.01KK290 pKa = 8.3VSSMLTVPGNSYY302 pKa = 10.36PFNAVDD308 pKa = 3.39ATSFATLNTIGDD320 pKa = 3.96LSFGGVGAYY329 pKa = 9.94LQGAPGSVTVGSFYY343 pKa = 11.02LDD345 pKa = 3.23YY346 pKa = 10.99DD347 pKa = 3.83IEE349 pKa = 4.6FKK351 pKa = 11.34SPIDD355 pKa = 3.57VQLNLL360 pKa = 3.94

Molecular weight: 38.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1080

221

499

360.0

40.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.426 ± 1.12

2.222 ± 0.347

4.63 ± 0.194

4.259 ± 1.452

5.556 ± 0.237

7.5 ± 0.958

1.574 ± 0.44

3.333 ± 0.232

4.167 ± 0.853

8.056 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.87 ± 0.342

3.241 ± 0.75

4.444 ± 0.563

2.778 ± 0.235

7.5 ± 1.387

9.074 ± 1.08

5.648 ± 1.072

9.259 ± 0.466

2.037 ± 0.253

3.426 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski