Schlegelella thermodepolymerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria;

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3542 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5SZV2|A0A2S5SZV2_9BURK UPF0056 membrane protein OS=Schlegelella thermodepolymerans OX=215580 GN=C1702_17830 PE=3 SV=1
MM1 pKa = 8.13GYY3 pKa = 8.86QQSGSITQSYY13 pKa = 7.88ATGAVRR19 pKa = 11.84GSAYY23 pKa = 10.71VGGLVGYY30 pKa = 9.41RR31 pKa = 11.84WAGSIADD38 pKa = 4.49SFWDD42 pKa = 3.72MEE44 pKa = 4.39TTEE47 pKa = 3.94QDD49 pKa = 3.35EE50 pKa = 4.5AVGNIGSALGATGLTTAQARR70 pKa = 11.84QASSYY75 pKa = 10.89AGWDD79 pKa = 3.57FDD81 pKa = 4.3TVWYY85 pKa = 8.98QEE87 pKa = 3.76RR88 pKa = 11.84DD89 pKa = 3.59LRR91 pKa = 11.84PMLRR95 pKa = 11.84AFAAPDD101 pKa = 3.48RR102 pKa = 11.84DD103 pKa = 3.32GDD105 pKa = 4.07GVYY108 pKa = 10.23EE109 pKa = 4.34ISNLYY114 pKa = 9.41QLQLMDD120 pKa = 3.18AWRR123 pKa = 11.84DD124 pKa = 3.35RR125 pKa = 11.84NYY127 pKa = 11.0VLIRR131 pKa = 11.84DD132 pKa = 3.89IDD134 pKa = 3.77ASATDD139 pKa = 3.45ATLPGFDD146 pKa = 4.9AAGIWSAQGFAPVGRR161 pKa = 11.84TDD163 pKa = 3.41QANSFQGSLDD173 pKa = 3.68GQGHH177 pKa = 6.33VISGLTIDD185 pKa = 5.53RR186 pKa = 11.84SGEE189 pKa = 3.9DD190 pKa = 3.51YY191 pKa = 11.32AGLFGRR197 pKa = 11.84TFDD200 pKa = 3.47ATIANVGLEE209 pKa = 4.26GGSVTGRR216 pKa = 11.84TFVGGLVGVQSGGSIAQSYY235 pKa = 7.79ATGAVAGLNAVGGLVGAQNGGSIAQSYY262 pKa = 7.79ATGAVEE268 pKa = 4.04GTLDD272 pKa = 3.3VGGLVGYY279 pKa = 7.44QQSGSITQSYY289 pKa = 7.86ATGAVEE295 pKa = 4.04GTHH298 pKa = 6.17RR299 pKa = 11.84VGGLVGLQGGGSIAQSYY316 pKa = 7.79ATGAVEE322 pKa = 4.22GSNFVGGLVGLQDD335 pKa = 3.87GGSIEE340 pKa = 3.97QSYY343 pKa = 7.93ATGAVSGRR351 pKa = 11.84DD352 pKa = 3.53YY353 pKa = 11.63VGGLVGRR360 pKa = 11.84QLGGSITQSYY370 pKa = 7.84ATGAVSGRR378 pKa = 11.84DD379 pKa = 3.53YY380 pKa = 11.63VGGLVGYY387 pKa = 7.32QQSGSITQSYY397 pKa = 7.85ATGAVAGDD405 pKa = 3.93WYY407 pKa = 10.37IGGLVGYY414 pKa = 9.13QWGGSIADD422 pKa = 4.09SFWDD426 pKa = 4.4RR427 pKa = 11.84NTTQQDD433 pKa = 3.49EE434 pKa = 4.5AVGNIGSASGATGLTTAQMQDD455 pKa = 2.97PFTFIDD461 pKa = 3.9AGWDD465 pKa = 3.66FAAIWGKK472 pKa = 10.93SSLNEE477 pKa = 3.66NGGYY481 pKa = 9.4MMLRR485 pKa = 11.84GVGPGTLYY493 pKa = 11.04DD494 pKa = 3.94DD495 pKa = 4.51YY496 pKa = 11.86VRR498 pKa = 11.84VAAVDD503 pKa = 3.75LSRR506 pKa = 11.84VYY508 pKa = 10.88GSSNPALAPHH518 pKa = 5.36VTIAGVGAANVSVGWGSAVGPQANAGAYY546 pKa = 9.86AFTDD550 pKa = 3.63PGVLDD555 pKa = 4.48ISTGSPGGVYY565 pKa = 10.11VDD567 pKa = 4.27YY568 pKa = 11.39GSGTLTVTPRR578 pKa = 11.84AITVTASNLSRR589 pKa = 11.84LYY591 pKa = 11.26GEE593 pKa = 5.28ANPALTFGPIGGDD606 pKa = 3.28GLASFHH612 pKa = 7.27ADD614 pKa = 3.15LAAAGFGLVTAAGMQSDD631 pKa = 4.16VGSYY635 pKa = 10.76AIDD638 pKa = 3.31LTGANGNYY646 pKa = 10.19DD647 pKa = 3.51VTFTPGTLTVTPRR660 pKa = 11.84AITVTADD667 pKa = 3.86DD668 pKa = 4.09LRR670 pKa = 11.84KK671 pKa = 9.94LLGQADD677 pKa = 4.09PALTWSITGGSLASFDD693 pKa = 3.81TEE695 pKa = 3.97AGVFGGDD702 pKa = 3.6LMRR705 pKa = 11.84DD706 pKa = 3.4PGEE709 pKa = 4.31GVGTYY714 pKa = 9.98SIRR717 pKa = 11.84QGTLVANSNYY727 pKa = 10.57DD728 pKa = 3.27VTFVDD733 pKa = 4.21GTLTIAPVAVSVASLFEE750 pKa = 4.05RR751 pKa = 11.84ALPLSLQADD760 pKa = 3.98PTEE763 pKa = 4.6GGALVLCDD771 pKa = 3.29TGVLPEE777 pKa = 4.82AEE779 pKa = 4.42CAAYY783 pKa = 8.23PHH785 pKa = 6.89PANRR789 pKa = 11.84GLAHH793 pKa = 6.63VGFAQQ798 pKa = 4.03

Molecular weight: 81.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5T1T2|A0A2S5T1T2_9BURK Outer membrane lipid asymmetry maintenance protein MlaD OS=Schlegelella thermodepolymerans OX=215580 GN=mlaD PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.34TRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 9.24VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3542

0

3542

1154366

26

3724

325.9

35.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.976 ± 0.057

0.959 ± 0.014

5.123 ± 0.027

5.829 ± 0.036

3.322 ± 0.026

8.309 ± 0.038

2.396 ± 0.024

3.971 ± 0.032

2.718 ± 0.04

11.0 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.019

2.142 ± 0.022

5.717 ± 0.034

3.996 ± 0.025

8.018 ± 0.041

4.574 ± 0.027

4.813 ± 0.023

8.022 ± 0.04

1.542 ± 0.019

2.292 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski